Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 549 |
| Acc65I | GGTACC | 1 cut(s) | 1675 |
| AccB1I | GGYRCC | 1 cut(s) | 1675 |
| AccBSI | CCGCTC | 1 cut(s) | 1801 |
| AccI | GTMKAC | 3 cut(s) | 623, 1482, 2763 |
| AccIII | TCCGGA | 1 cut(s) | 2540 |
| AciI | CCGC | 7 cut(s) | 178, 839, 1346, 1793, 1801, 2363, 2739 |
| AclWI | GGATC | 3 cut(s) | 19, 295, 2219 |
| AcoI | YGGCCR | 4 cut(s) | 711, 836, 2364, 2751 |
| AcuI | CTGAAG | 3 cut(s) | 153, 1293, 1683 |
| AcvI | CACGTG | 1 cut(s) | 77 |
| AfaI | GTAC | 9 cut(s) | 321, 1156, 1192, 1266, 1281, 1558, 1677, 2616, 2682 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 633, 2391, 2518 |
| AflII | CTTAAG | 1 cut(s) | 1196 |
| AflIII | ACRYGT | 1 cut(s) | 2304 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2633 |
| AhlI | ACTAGT | 1 cut(s) | 2339 |
| AjnI | CCWGG | 3 cut(s) | 781, 1531, 2134 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1038, 1070 |
| Alw21I | GWGCWC | 2 cut(s) | 501, 2548 |
| Alw26I | GTCTC | 6 cut(s) | 309, 688, 899, 1310, 2283, 2558 |
| AlwI | GGATC | 3 cut(s) | 19, 295, 2219 |
| Ama87I | CYCGRG | 1 cut(s) | 2489 |
| Aor13HI | TCCGGA | 1 cut(s) | 2540 |
| AoxI | GGCC | 7 cut(s) | 711, 836, 900, 1535, 2147, 2364, 2751 |
| ApeKI | GCWGC | 4 cut(s) | 1061, 1499, 1790, 2068 |
| AseI | ATTAAT | 2 cut(s) | 831, 2142 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2241 |
| Asp718I | GGTACC | 1 cut(s) | 1675 |
| AspA2I | CCTAGG | 1 cut(s) | 1679 |
| AspLEI | GCGC | 1 cut(s) | 550 |
| AspS9I | GGNCC | 1 cut(s) | 1535 |
| AsuC2I | CCSGG | 1 cut(s) | 2209 |
| AsuHPI | GGTGA | 6 cut(s) | 227, 248, 678, 2108, 2446, 2611 |
| AsuII | TTCGAA | 2 cut(s) | 1141, 1294 |
| AvaI | CYCGRG | 1 cut(s) | 2489 |
| AvrII | CCTAGG | 1 cut(s) | 1679 |
| AxyI | CCTNAGG | 1 cut(s) | 116 |
| BaeGI | GKGCMC | 1 cut(s) | 115 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1659, 1667, 1692, 1700 |
| BalI | TGGCCA | 2 cut(s) | 713, 2753 |
| BanI | GGYRCC | 1 cut(s) | 1675 |
| BanII | GRGCYC | 1 cut(s) | 2548 |
| BauI | CACGAG | 1 cut(s) | 1232 |
| BbrPI | CACGTG | 1 cut(s) | 77 |
| BbsI | GAAGAC | 2 cut(s) | 81, 1125 |
| Bbv12I | GWGCWC | 2 cut(s) | 501, 2548 |
| BbvCI | CCTCAGC | 1 cut(s) | 849 |
| BbvI | GCAGC | 4 cut(s) | 1073, 1511, 1777, 2080 |
| BccI | CCATC | 2 cut(s) | 1718, 2279 |
| BceAI | ACGGC | 1 cut(s) | 2756 |
| BciT130I | CCWGG | 3 cut(s) | 783, 1533, 2136 |
| BclI | TGATCA | 2 cut(s) | 430, 609 |
| BcnI | CCSGG | 1 cut(s) | 2209 |
| BcoDI | GTCTC | 6 cut(s) | 309, 688, 899, 1310, 2283, 2558 |
| BcuI | ACTAGT | 1 cut(s) | 2339 |
| BfmI | CTRYAG | 3 cut(s) | 256, 789, 1311 |
| BfrI | CTTAAG | 1 cut(s) | 1196 |
| BglII | AGATCT | 1 cut(s) | 1103 |
| BisI | GCNGC | 7 cut(s) | 839, 1062, 1500, 1791, 2069, 2364, 2739 |
| BlnI | CCTAGG | 1 cut(s) | 1679 |
| BlsI | GCNGC | 7 cut(s) | 840, 1063, 1501, 1792, 2070, 2365, 2740 |
| Bme1390I | CCNGG | 4 cut(s) | 783, 1533, 2136, 2209 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2633 |
| BmeT110I | CYCGRG | 1 cut(s) | 2489 |
| BmgT120I | GGNCC | 1 cut(s) | 1535 |
| BmiI | GGNNCC | 4 cut(s) | 1581, 1677, 1882, 2737 |
| BmrFI | CCNGG | 4 cut(s) | 783, 1533, 2136, 2209 |
| BmsI | GCATC | 6 cut(s) | 70, 118, 181, 458, 559, 1732 |
| BpiI | GAAGAC | 2 cut(s) | 81, 1125 |
| BpmI | CTGGAG | 1 cut(s) | 2093 |
| Bpu10I | CCTNAGC | 4 cut(s) | 492, 849, 1842, 2387 |
| Bpu14I | TTCGAA | 2 cut(s) | 1141, 1294 |
| BpuEI | CTTGAG | 5 cut(s) | 40, 741, 1784, 2136, 2693 |
| BpuMI | CCSGG | 1 cut(s) | 2209 |
| Bsa29I | ATCGAT | 2 cut(s) | 370, 1737 |
| BsaAI | YACGTR | 1 cut(s) | 77 |
| BsaBI | GATNNNNATC | 2 cut(s) | 472, 1822 |
| BsaI | GGTCTC | 2 cut(s) | 688, 1310 |
| BsaJI | CCNNGG | 7 cut(s) | 198, 1531, 1532, 1679, 1884, 1908, 2643 |
| BsaWI | WCCGGW | 2 cut(s) | 2540, 2621 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 2085, 2115, 2535, 2565 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 633, 2391, 2518 |
| Bse1I | ACTGG | 2 cut(s) | 2257, 2367 |
| Bse21I | CCTNAGG | 1 cut(s) | 116 |
| Bse3DI | GCAATG | 2 cut(s) | 624, 1938 |
| Bse8I | GATNNNNATC | 2 cut(s) | 472, 1822 |
| BseAI | TCCGGA | 1 cut(s) | 2540 |
| BseBI | CCWGG | 3 cut(s) | 783, 1533, 2136 |
| BseCI | ATCGAT | 2 cut(s) | 370, 1737 |
| BseDI | CCNNGG | 7 cut(s) | 198, 1531, 1532, 1679, 1884, 1908, 2643 |
| BseGI | GGATG | 3 cut(s) | 1552, 1828, 1918 |
| BseJI | GATNNNNATC | 2 cut(s) | 472, 1822 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 633, 2391, 2518 |
| BseMI | GCAATG | 2 cut(s) | 624, 1938 |
| BseMII | CTCAG | 5 cut(s) | 356, 455, 840, 888, 2378 |
| BseNI | ACTGG | 2 cut(s) | 2257, 2367 |
| BseRI | GAGGAG | 1 cut(s) | 1256 |
| BseSI | GKGCMC | 1 cut(s) | 115 |
| BseXI | GCAGC | 4 cut(s) | 1073, 1511, 1777, 2080 |
| BsgI | GTGCAG | 1 cut(s) | 1080 |
| Bsh1285I | CGRYCG | 1 cut(s) | 159 |
| BshFI | GGCC | 7 cut(s) | 713, 838, 902, 1537, 2149, 2366, 2753 |
| BshNI | GGYRCC | 1 cut(s) | 1675 |
| BshVI | ATCGAT | 2 cut(s) | 370, 1737 |
| BsiEI | CGRYCG | 1 cut(s) | 159 |
| BsiHKAI | GWGCWC | 2 cut(s) | 501, 2548 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2489 |
| BsiSI | CCGG | 3 cut(s) | 2209, 2541, 2622 |
| BslFI | GGGAC | 5 cut(s) | 134, 650, 683, 1001, 2573 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 633, 2391, 2518 |
| BsmAI | GTCTC | 6 cut(s) | 309, 688, 899, 1310, 2283, 2558 |
| BsmBI | CGTCTC | 3 cut(s) | 309, 2283, 2558 |
| BsmFI | GGGAC | 5 cut(s) | 134, 650, 683, 1001, 2573 |
| BsmI | GAATGC | 1 cut(s) | 2443 |
| BsnI | GGCC | 7 cut(s) | 713, 838, 902, 1537, 2149, 2366, 2753 |
| Bso31I | GGTCTC | 2 cut(s) | 688, 1310 |
| BsoBI | CYCGRG | 1 cut(s) | 2489 |
| Bsp119I | TTCGAA | 2 cut(s) | 1141, 1294 |
| Bsp1286I | GDGCHC | 3 cut(s) | 115, 501, 2548 |
| Bsp13I | TCCGGA | 1 cut(s) | 2540 |
| Bsp19I | CCATGG | 2 cut(s) | 1884, 1908 |
| BspACI | CCGC | 7 cut(s) | 178, 839, 1346, 1793, 1801, 2363, 2739 |
| BspANI | GGCC | 7 cut(s) | 713, 838, 902, 1537, 2149, 2366, 2753 |
| BspCNI | CTCAG | 5 cut(s) | 355, 456, 841, 889, 2379 |
| BspDI | ATCGAT | 2 cut(s) | 370, 1737 |
| BspEI | TCCGGA | 1 cut(s) | 2540 |
| BspHI | TCATGA | 2 cut(s) | 204, 1806 |
| BspLI | GGNNCC | 4 cut(s) | 1581, 1677, 1882, 2737 |
| BspMAI | CTGCAG | 1 cut(s) | 1315 |
| BspPI | GGATC | 3 cut(s) | 19, 295, 2219 |
| BspT104I | TTCGAA | 2 cut(s) | 1141, 1294 |
| BspT107I | GGYRCC | 1 cut(s) | 1675 |
| BspTI | CTTAAG | 1 cut(s) | 1196 |
| BspTNI | GGTCTC | 2 cut(s) | 688, 1310 |
| BsrBI | CCGCTC | 1 cut(s) | 1801 |
| BsrDI | GCAATG | 2 cut(s) | 624, 1938 |
| BsrI | ACTGG | 2 cut(s) | 2257, 2367 |
| BssECI | CCNNGG | 7 cut(s) | 198, 1531, 1532, 1679, 1884, 1908, 2643 |
| BssSI | CACGAG | 1 cut(s) | 1232 |
| BssT1I | CCWWGG | 5 cut(s) | 198, 1679, 1884, 1908, 2643 |
| Bst2BI | CACGAG | 1 cut(s) | 1232 |
| Bst2UI | CCWGG | 3 cut(s) | 783, 1533, 2136 |
| Bst4CI | ACNGT | 8 cut(s) | 542, 682, 956, 1784, 2114, 2166, 2569, 2761 |
| Bst6I | CTCTTC | 1 cut(s) | 1978 |
| BstAFI | CTTAAG | 1 cut(s) | 1196 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 194, 2030 |
| BstBAI | YACGTR | 1 cut(s) | 77 |
| BstBI | TTCGAA | 2 cut(s) | 1141, 1294 |
| BstC8I | GCNNGC | 2 cut(s) | 339, 520 |
| BstDEI | CTNAG | 9 cut(s) | 116, 342, 464, 492, 849, 897, 1842, 1944, 2387 |
| BstDSI | CCRYGG | 2 cut(s) | 1884, 1908 |
| BstEII | GGTNACC | 1 cut(s) | 1357 |
| BstF5I | GGATG | 3 cut(s) | 1552, 1828, 1918 |
| BstHHI | GCGC | 1 cut(s) | 550 |
| BstMAI | GTCTC | 6 cut(s) | 309, 688, 899, 1310, 2283, 2558 |
| BstMCI | CGRYCG | 1 cut(s) | 159 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 194, 719, 1070, 1283, 2030, 2074 |
| BstNI | CCWGG | 3 cut(s) | 783, 1533, 2136 |
| BstNSI | RCATGY | 3 cut(s) | 341, 922, 2308 |
| BstPI | GGTNACC | 1 cut(s) | 1357 |
| BstSCI | CCNGG | 4 cut(s) | 781, 1531, 2134, 2207 |
| BstSFI | CTRYAG | 3 cut(s) | 256, 789, 1311 |
| BstSLI | GKGCMC | 1 cut(s) | 115 |
| BstV1I | GCAGC | 4 cut(s) | 1073, 1511, 1777, 2080 |
| BstV2I | GAAGAC | 2 cut(s) | 81, 1125 |
| BstX2I | RGATCY | 2 cut(s) | 300, 1103 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 697 |
| BstYI | RGATCY | 2 cut(s) | 300, 1103 |
| Bsu15I | ATCGAT | 2 cut(s) | 370, 1737 |
| Bsu36I | CCTNAGG | 1 cut(s) | 116 |
| BsuRI | GGCC | 7 cut(s) | 713, 838, 902, 1537, 2149, 2366, 2753 |
| BsuTUI | ATCGAT | 2 cut(s) | 370, 1737 |
| BtgI | CCRYGG | 2 cut(s) | 1884, 1908 |
| BtgZI | GCGATG | 2 cut(s) | 260, 2752 |
| BtsCI | GGATG | 3 cut(s) | 1552, 1828, 1918 |
| BtsI | GCAGTG | 2 cut(s) | 450, 1950 |
| BtsIMutI | CAGTG | 8 cut(s) | 123, 337, 350, 450, 1528, 1950, 2119, 2374 |
| Cac8I | GCNNGC | 2 cut(s) | 339, 520 |
| CciI | TCATGA | 2 cut(s) | 204, 1806 |
| CfoI | GCGC | 1 cut(s) | 550 |
| Cfr13I | GGNCC | 1 cut(s) | 1535 |
| ClaI | ATCGAT | 2 cut(s) | 370, 1737 |
| CseI | GACGC | 1 cut(s) | 194 |
| Csp6I | GTAC | 9 cut(s) | 320, 1155, 1191, 1265, 1280, 1557, 1676, 2615, 2681 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2323, 2358 |
| CviQI | GTAC | 9 cut(s) | 320, 1155, 1191, 1265, 1280, 1557, 1676, 2615, 2681 |
| DdeI | CTNAG | 9 cut(s) | 116, 342, 464, 492, 849, 897, 1842, 1944, 2387 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2633 |
| EaeI | YGGCCR | 4 cut(s) | 711, 836, 2364, 2751 |
| Eam1104I | CTCTTC | 1 cut(s) | 1978 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2633 |
| EarI | CTCTTC | 1 cut(s) | 1978 |
| Ecl136II | GAGCTC | 1 cut(s) | 2546 |
| Eco130I | CCWWGG | 5 cut(s) | 198, 1679, 1884, 1908, 2643 |
| Eco24I | GRGCYC | 1 cut(s) | 2548 |
| Eco31I | GGTCTC | 2 cut(s) | 688, 1310 |
| Eco53kI | GAGCTC | 1 cut(s) | 2546 |
| Eco57I | CTGAAG | 3 cut(s) | 153, 1293, 1683 |
| Eco72I | CACGTG | 1 cut(s) | 77 |
| Eco81I | CCTNAGG | 1 cut(s) | 116 |
| Eco88I | CYCGRG | 1 cut(s) | 2489 |
| Eco91I | GGTNACC | 1 cut(s) | 1357 |
| EcoICRI | GAGCTC | 1 cut(s) | 2546 |
| EcoO65I | GGTNACC | 1 cut(s) | 1357 |
| EcoRI | GAATTC | 4 cut(s) | 261, 308, 854, 2776 |
| EcoRII | CCWGG | 3 cut(s) | 781, 1531, 2134 |
| EcoT14I | CCWWGG | 5 cut(s) | 198, 1679, 1884, 1908, 2643 |
| EcoT22I | ATGCAT | 2 cut(s) | 174, 1747 |
| EcoT38I | GRGCYC | 1 cut(s) | 2548 |
| ErhI | CCWWGG | 5 cut(s) | 198, 1679, 1884, 1908, 2643 |
| Esp3I | CGTCTC | 3 cut(s) | 309, 2283, 2558 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1038, 1070 |
| FaqI | GGGAC | 5 cut(s) | 134, 650, 683, 1001, 2573 |
| FauI | CCCGC | 1 cut(s) | 1339 |
| FbaI | TGATCA | 2 cut(s) | 430, 609 |
| FblI | GTMKAC | 3 cut(s) | 623, 1482, 2763 |
| Fnu4HI | GCNGC | 7 cut(s) | 839, 1062, 1500, 1791, 2069, 2364, 2739 |
| FokI | GGATG | 3 cut(s) | 1559, 1815, 1925 |
| FriOI | GRGCYC | 1 cut(s) | 2548 |
| Fsp4HI | GCNGC | 7 cut(s) | 839, 1062, 1500, 1791, 2069, 2364, 2739 |
| FspI | TGCGCA | 1 cut(s) | 549 |
| GlaI | GCGC | 1 cut(s) | 549 |
| GluI | GCNGC | 7 cut(s) | 839, 1062, 1500, 1791, 2069, 2364, 2739 |
| GsuI | CTGGAG | 1 cut(s) | 2093 |
| HaeIII | GGCC | 7 cut(s) | 713, 838, 902, 1537, 2149, 2366, 2753 |
| HapII | CCGG | 3 cut(s) | 2209, 2541, 2622 |
| HgaI | GACGC | 1 cut(s) | 194 |
| HhaI | GCGC | 1 cut(s) | 550 |
| Hin6I | GCGC | 1 cut(s) | 548 |
| HinP1I | GCGC | 1 cut(s) | 548 |
| HincII | GTYRAC | 3 cut(s) | 1849, 2254, 2715 |
| HindII | GTYRAC | 3 cut(s) | 1849, 2254, 2715 |
| HindIII | AAGCTT | 2 cut(s) | 58, 1328 |
| HinfI | GANTC | 8 cut(s) | 948, 1130, 1213, 1249, 1823, 1921, 2086, 2487 |
| HpaI | GTTAAC | 2 cut(s) | 2254, 2715 |
| HpaII | CCGG | 3 cut(s) | 2209, 2541, 2622 |
| HphI | GGTGA | 6 cut(s) | 227, 248, 678, 2108, 2446, 2611 |
| Hpy99I | CGWCG | 2 cut(s) | 1784, 2735 |
| HpyAV | CCTTC | 5 cut(s) | 697, 1137, 1347, 1382, 2291 |
| HpyCH4III | ACNGT | 8 cut(s) | 542, 682, 956, 1784, 2114, 2166, 2569, 2761 |
| HpyCH4IV | ACGT | 5 cut(s) | 76, 318, 554, 2292, 2730 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 194, 719, 1070, 1283, 2030, 2074 |
| HpyF3I | CTNAG | 9 cut(s) | 116, 342, 464, 492, 849, 897, 1842, 1944, 2387 |
| HpySE526I | ACGT | 5 cut(s) | 76, 318, 554, 2292, 2730 |
| HspAI | GCGC | 1 cut(s) | 548 |
| Kpn2I | TCCGGA | 1 cut(s) | 2540 |
| KpnI | GGTACC | 1 cut(s) | 1679 |
| Ksp22I | TGATCA | 2 cut(s) | 430, 609 |
| KspAI | GTTAAC | 2 cut(s) | 2254, 2715 |
| LmnI | GCTCC | 5 cut(s) | 1585, 1798, 2074, 2543, 2735 |
| Lsp1109I | GCAGC | 4 cut(s) | 1073, 1511, 1777, 2080 |
| LweI | GCATC | 6 cut(s) | 70, 118, 181, 458, 559, 1732 |
| MaeII | ACGT | 5 cut(s) | 76, 318, 554, 2292, 2730 |
| MbiI | CCGCTC | 1 cut(s) | 1801 |
| MfeI | CAATTG | 1 cut(s) | 1541 |
| MflI | RGATCY | 2 cut(s) | 300, 1103 |
| MhlI | GDGCHC | 3 cut(s) | 115, 501, 2548 |
| MlsI | TGGCCA | 2 cut(s) | 713, 2753 |
| MluNI | TGGCCA | 2 cut(s) | 713, 2753 |
| MlyI | GAGTC | 1 cut(s) | 2481 |
| MmeI | TCCRAC | 3 cut(s) | 390, 2083, 2713 |
| Mox20I | TGGCCA | 2 cut(s) | 713, 2753 |
| Mph1103I | ATGCAT | 2 cut(s) | 174, 1747 |
| MroI | TCCGGA | 1 cut(s) | 2540 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2241 |
| MscI | TGGCCA | 2 cut(s) | 713, 2753 |
| MslI | CAYNNNNRTG | 7 cut(s) | 75, 395, 432, 1041, 1446, 2309, 2771 |
| Msp20I | TGGCCA | 2 cut(s) | 713, 2753 |
| MspA1I | CMGCKG | 2 cut(s) | 1346, 2071 |
| MspCI | CTTAAG | 1 cut(s) | 1196 |
| MspI | CCGG | 3 cut(s) | 2209, 2541, 2622 |
| MspR9I | CCNGG | 4 cut(s) | 783, 1533, 2136, 2209 |
| MunI | CAATTG | 1 cut(s) | 1541 |
| Mva1269I | GAATGC | 1 cut(s) | 2443 |
| MvaI | CCWGG | 3 cut(s) | 783, 1533, 2136 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 194, 719, 1070, 1283, 2030, 2074 |
| NciI | CCSGG | 1 cut(s) | 2209 |
| NcoI | CCATGG | 2 cut(s) | 1884, 1908 |
| NlaIV | GGNNCC | 4 cut(s) | 1581, 1677, 1882, 2737 |
| NmeAIII | GCCGAG | 1 cut(s) | 928 |
| NmuCI | GTSAC | 3 cut(s) | 859, 1666, 2452 |
| NsbI | TGCGCA | 1 cut(s) | 549 |
| NsiI | ATGCAT | 2 cut(s) | 174, 1747 |
| NspI | RCATGY | 3 cut(s) | 341, 922, 2308 |
| NspV | TTCGAA | 2 cut(s) | 1141, 1294 |
| PaeI | GCATGC | 1 cut(s) | 341 |
| PaeR7I | CTCGAG | 1 cut(s) | 2489 |
| PagI | TCATGA | 2 cut(s) | 204, 1806 |
| PasI | CCCWGGG | 1 cut(s) | 1532 |
| PciI | ACATGT | 1 cut(s) | 2304 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2496 |
| PctI | GAATGC | 1 cut(s) | 2443 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2241 |
| PfeI | GAWTC | 7 cut(s) | 948, 1130, 1213, 1249, 1823, 1921, 2086 |
| PflFI | GACNNNGTC | 1 cut(s) | 1892 |
| PkrI | GCNGC | 7 cut(s) | 840, 1063, 1501, 1792, 2070, 2365, 2740 |
| Ple19I | CGATCG | 1 cut(s) | 159 |
| PleI | GAGTC | 1 cut(s) | 2481 |
| PmaCI | CACGTG | 1 cut(s) | 77 |
| PmlI | CACGTG | 1 cut(s) | 77 |
| PpsI | GAGTC | 1 cut(s) | 2481 |
| Ppu21I | YACGTR | 1 cut(s) | 77 |
| PscI | ACATGT | 1 cut(s) | 2304 |
| PshBI | ATTAAT | 2 cut(s) | 831, 2142 |
| Psp124BI | GAGCTC | 1 cut(s) | 2548 |
| Psp6I | CCWGG | 3 cut(s) | 781, 1531, 2134 |
| PspCI | CACGTG | 1 cut(s) | 77 |
| PspEI | GGTNACC | 1 cut(s) | 1357 |
| PspGI | CCWGG | 3 cut(s) | 781, 1531, 2134 |
| PspN4I | GGNNCC | 4 cut(s) | 1581, 1677, 1882, 2737 |
| PspPI | GGNCC | 1 cut(s) | 1535 |
| PspXI | VCTCGAGB | 1 cut(s) | 2489 |
| PstI | CTGCAG | 1 cut(s) | 1315 |
| PsuI | RGATCY | 2 cut(s) | 300, 1103 |
| PsyI | GACNNNGTC | 1 cut(s) | 1892 |
| PvuI | CGATCG | 1 cut(s) | 159 |
| PvuII | CAGCTG | 1 cut(s) | 2071 |
| RsaI | GTAC | 9 cut(s) | 321, 1156, 1192, 1266, 1281, 1558, 1677, 2616, 2682 |
| RsaNI | GTAC | 9 cut(s) | 320, 1155, 1191, 1265, 1280, 1557, 1676, 2615, 2681 |
| RseI | CAYNNNNRTG | 7 cut(s) | 75, 395, 432, 1041, 1446, 2309, 2771 |
| SacI | GAGCTC | 1 cut(s) | 2548 |
| SatI | GCNGC | 7 cut(s) | 839, 1062, 1500, 1791, 2069, 2364, 2739 |
| Sau96I | GGNCC | 1 cut(s) | 1535 |
| SchI | GAGTC | 1 cut(s) | 2481 |
| ScrFI | CCNGG | 4 cut(s) | 783, 1533, 2136, 2209 |
| SduI | GDGCHC | 3 cut(s) | 115, 501, 2548 |
| SfaNI | GCATC | 6 cut(s) | 70, 118, 181, 458, 559, 1732 |
| SfcI | CTRYAG | 3 cut(s) | 256, 789, 1311 |
| Sfr274I | CTCGAG | 1 cut(s) | 2489 |
| SfuI | TTCGAA | 2 cut(s) | 1141, 1294 |
| SlaI | CTCGAG | 1 cut(s) | 2489 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 75, 395, 432, 1041, 1446, 2309, 2771 |
| SmlI | CTYRAG | 7 cut(s) | 55, 756, 1196, 1763, 2151, 2489, 2708 |
| SmoI | CTYRAG | 7 cut(s) | 55, 756, 1196, 1763, 2151, 2489, 2708 |
| SpeI | ACTAGT | 1 cut(s) | 2339 |
| SphI | GCATGC | 1 cut(s) | 341 |
| SsiI | CCGC | 7 cut(s) | 178, 839, 1346, 1793, 1801, 2363, 2739 |
| SstI | GAGCTC | 1 cut(s) | 2548 |
| StyD4I | CCNGG | 4 cut(s) | 781, 1531, 2134, 2207 |
| StyI | CCWWGG | 5 cut(s) | 198, 1679, 1884, 1908, 2643 |
| TaaI | ACNGT | 8 cut(s) | 542, 682, 956, 1784, 2114, 2166, 2569, 2761 |
| TaiI | ACGT | 5 cut(s) | 79, 321, 557, 2295, 2733 |
| TaqI | TCGA | 9 cut(s) | 370, 501, 966, 1141, 1294, 1737, 2472, 2490, 2499 |
| TatI | WGTACW | 3 cut(s) | 1154, 1190, 1279 |
| TauI | GCSGC | 3 cut(s) | 841, 2366, 2741 |
| TfiI | GAWTC | 7 cut(s) | 948, 1130, 1213, 1249, 1823, 1921, 2086 |
| TscAI | CASTG | 8 cut(s) | 130, 337, 350, 450, 1528, 1957, 2119, 2374 |
| TseFI | GTSAC | 3 cut(s) | 859, 1666, 2452 |
| TseI | GCWGC | 4 cut(s) | 1061, 1499, 1790, 2068 |
| Tsp45I | GTSAC | 3 cut(s) | 859, 1666, 2452 |
| TspGWI | ACGGA | 4 cut(s) | 687, 1604, 2068, 2291 |
| TspRI | CASTG | 8 cut(s) | 130, 337, 350, 450, 1528, 1957, 2119, 2374 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1892 |
| Vha464I | CTTAAG | 1 cut(s) | 1196 |
| VspI | ATTAAT | 2 cut(s) | 831, 2142 |
| XceI | RCATGY | 3 cut(s) | 341, 922, 2308 |
| XhoI | CTCGAG | 1 cut(s) | 2489 |
| XmaJI | CCTAGG | 1 cut(s) | 1679 |
| XmiI | GTMKAC | 3 cut(s) | 623, 1482, 2763 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2241 |
| Zsp2I | ATGCAT | 2 cut(s) | 174, 1747 |