Rorug07G0013600

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000912
Physical Location & Seq
Reverse (-)
1048905 .. 1060829
11925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0013600.1

Sequence Viewer

Length: 966 bp
ATGATGGAGTGGAGACCGATAATCTTGACGGAGCCGGAGGCCAATGACGTGTTAAGGCAAGTCCAATCCAAAGGGGTGACGATCGCTCTAGATGGATTATCTCGCGCAAAAGCCGTCGAGGCTATCGATTCGTTCCTCAAACAATACAAGGAATGCTTTTGGACAATAGTGATCAGCGAGATTACCTTTGCGATCAGAGAAGAGCTCCTAAAATCTAGGAATGTGATCGACGTAAAGTTGGTTAGAGAAGTTGTCGAGTATCTTTCAAACCGCCGCGGCGTGGAGAAGAAGAAGGAGGATGAGGAGGAGGAGAGCTCGGAGGGCTCTGGGGAGGAGTCTGAAGATGAAGAAGATTCGGATCAGGATTTGAAAAAGGAAGAGGAATCGGATGAGGAGATAGATGATTTAAAAGATGATCCAATAACGAGACAAGAATGCATCGAGGATTTGTTGGCACTGTCCAGGGAGAAGGGATTGAGTGTGAAAGAAGGCGAGGAGGATGAGATCCTGGATATGATGATTCCCTACCTCCGCGGATGCCTTATGTATACCAAGGGCATCATGTTTTTCCTGAAAAGGGTGAGAGAGCATATCGATAGGTGTAGCACCCTCAACGATTTGGAGACGGTGAAGGCCGCCCTCGAGAGTAGCATTTTTCGTGAAGAATATGAGGACTGGAAAAAGGACAAGAGGGAGGGGGATATGGCTGATGCTGAGGACCAAGTTAATCAACTGTTAAAAAAGAATATGGGACCAACTGAAGAAGAAGGGATTGCTGCCCCCGTGAACCAGATAGAAGAAGAAAAAAGGGTATGTAGAGATATAACGGAAGTTGAAGAAAAGGAGACTTTGACCCCCTTGAACCTGCCAGAACAAGCAAAAGAAAGTATGGGCAATGCTTATTCCTGCATCAAAGAAAGTTTGGAAAGGGGTGGAGCTAATAAGAGAAAGCTTGCTCAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

321

Amino Acids

36.97

Weight (kDa)

4.52

Isoelectric Point (pI)

62.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 873
AccI GTMKAC 1 cut(s) 548
AccII CGCG 3 cut(s) 105, 276, 534
AciI CCGC 6 cut(s) 271, 274, 276, 532, 534, 636
AclWI GGATC 3 cut(s) 366, 410, 499
AcuI CTGAAG 2 cut(s) 360, 780
AfiI CCNNNNNNNGG 2 cut(s) 280, 577
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 4 cut(s) 267, 370, 836, 862
AjiI CACGTC 1 cut(s) 49
AjnI CCWGG 2 cut(s) 461, 507
AluBI AGCT 4 cut(s) 205, 315, 938, 952
AluI AGCT 4 cut(s) 205, 315, 938, 952
Alw21I GWGCWC 2 cut(s) 207, 317
Alw26I GTCTC 4 cut(s) 7, 421, 617, 839
AlwI GGATC 3 cut(s) 366, 410, 499
Ama87I CYCGRG 1 cut(s) 641
AoxI GGCC 2 cut(s) 39, 633
ApeKI GCWGC 1 cut(s) 776
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 2 cut(s) 718, 752
AsuHPI GGTGA 3 cut(s) 88, 592, 640
AvaI CYCGRG 1 cut(s) 641
AvaII GGWCC 2 cut(s) 718, 752
BanII GRGCYC 3 cut(s) 207, 317, 326
Bbv12I GWGCWC 2 cut(s) 207, 317
BbvCI CCTCAGC 1 cut(s) 714
BbvI GCAGC 1 cut(s) 763
BccI CCATC 1 cut(s) 86
BceAI ACGGC 1 cut(s) 98
BciT130I CCWGG 2 cut(s) 463, 509
BclI TGATCA 1 cut(s) 171
BcoDI GTCTC 4 cut(s) 7, 421, 617, 839
BfaI CTAG 2 cut(s) 89, 216
BfuAI ACCTGC 1 cut(s) 873
BglI GCCNNNNNGGC 1 cut(s) 119
BglII AGATCT 1 cut(s) 960
BisI GCNGC 4 cut(s) 274, 277, 636, 777
BlsI GCNGC 4 cut(s) 275, 278, 637, 778
Bme1390I CCNGG 2 cut(s) 463, 509
Bme18I GGWCC 2 cut(s) 718, 752
BmeT110I CYCGRG 1 cut(s) 641
BmgBI CACGTC 1 cut(s) 49
BmgT120I GGNCC 2 cut(s) 718, 752
BmiI GGNNCC 2 cut(s) 33, 753
BmrFI CCNGG 2 cut(s) 463, 509
BmsI GCATC 5 cut(s) 447, 527, 567, 700, 918
BplI GAGNNNNNCTC 4 cut(s) 189, 221, 299, 331
Bpu10I CCTNAGC 1 cut(s) 714
Bsa29I ATCGAT 2 cut(s) 126, 594
BsaBI GATNNNNATC 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 7
BsaJI CCNNGG 4 cut(s) 274, 462, 532, 552
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 577
Bse1I ACTGG 1 cut(s) 680
Bse3DI GCAATG 1 cut(s) 901
Bse8I GATNNNNATC 1 cut(s) 357
BseBI CCWGG 2 cut(s) 463, 509
BseCI ATCGAT 2 cut(s) 126, 594
BseDI CCNNGG 4 cut(s) 274, 462, 532, 552
BseGI GGATG 4 cut(s) 304, 394, 505, 542
BseJI GATNNNNATC 1 cut(s) 357
BseLI CCNNNNNNNGG 2 cut(s) 280, 577
BseMI GCAATG 1 cut(s) 901
BseMII CTCAG 1 cut(s) 705
BseNI ACTGG 1 cut(s) 680
BseRI GAGGAG 6 cut(s) 317, 320, 323, 347, 407, 509
BseXI GCAGC 1 cut(s) 763
Bsh1236I CGCG 3 cut(s) 105, 276, 534
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 2 cut(s) 41, 635
BshVI ATCGAT 2 cut(s) 126, 594
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 2 cut(s) 207, 317
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 1 cut(s) 765
BslI CCNNNNNNNGG 2 cut(s) 280, 577
BsmAI GTCTC 4 cut(s) 7, 421, 617, 839
BsmBI CGTCTC 1 cut(s) 617
BsmFI GGGAC 1 cut(s) 765
BsmI GAATGC 2 cut(s) 158, 440
BsnI GGCC 2 cut(s) 41, 635
Bso31I GGTCTC 1 cut(s) 7
BsoBI CYCGRG 1 cut(s) 641
Bsp1286I GDGCHC 3 cut(s) 207, 317, 326
Bsp143I GATC 8 cut(s) 81, 171, 192, 225, 358, 415, 504, 960
BspACI CCGC 6 cut(s) 271, 274, 276, 532, 534, 636
BspANI GGCC 2 cut(s) 41, 635
BspCNI CTCAG 1 cut(s) 706
BspDI ATCGAT 2 cut(s) 126, 594
BspFNI CGCG 3 cut(s) 105, 276, 534
BspLI GGNNCC 2 cut(s) 33, 753
BspMI ACCTGC 1 cut(s) 873
BspPI GGATC 3 cut(s) 366, 410, 499
BspQI GCTCTTC 1 cut(s) 195
BspTNI GGTCTC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 901
BsrI ACTGG 1 cut(s) 680
BssECI CCNNGG 4 cut(s) 274, 462, 532, 552
BssMI GATC 8 cut(s) 81, 171, 192, 225, 358, 415, 504, 960
BssNAI GTATAC 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 552
Bst1107I GTATAC 1 cut(s) 549
Bst2UI CCWGG 2 cut(s) 463, 509
Bst4CI ACNGT 3 cut(s) 459, 628, 735
Bst6I CTCTTC 2 cut(s) 195, 372
BstC8I GCNNGC 1 cut(s) 954
BstDEI CTNAG 2 cut(s) 714, 957
BstDSI CCRYGG 2 cut(s) 274, 532
BstF5I GGATG 4 cut(s) 304, 394, 505, 542
BstFNI CGCG 3 cut(s) 105, 276, 534
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 8 cut(s) 84, 174, 195, 228, 361, 418, 507, 963
BstMAI GTCTC 4 cut(s) 7, 421, 617, 839
BstMBI GATC 8 cut(s) 81, 171, 192, 225, 358, 415, 504, 960
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 2 cut(s) 119, 321
BstNI CCWGG 2 cut(s) 463, 509
BstSCI CCNGG 2 cut(s) 461, 507
BstUI CGCG 3 cut(s) 105, 276, 534
BstV1I GCAGC 1 cut(s) 763
BstX2I RGATCY 2 cut(s) 504, 960
BstYI RGATCY 2 cut(s) 504, 960
BstZ17I GTATAC 1 cut(s) 549
Bsu15I ATCGAT 2 cut(s) 126, 594
BsuRI GGCC 2 cut(s) 41, 635
BsuTUI ATCGAT 2 cut(s) 126, 594
BtgI CCRYGG 2 cut(s) 274, 532
BtrI CACGTC 1 cut(s) 49
BtsCI GGATG 4 cut(s) 304, 394, 505, 542
BtsIMutI CAGTG 1 cut(s) 455
BveI ACCTGC 1 cut(s) 873
Cac8I GCNNGC 1 cut(s) 954
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 718, 752
Cfr42I CCGCGG 2 cut(s) 277, 535
ClaI ATCGAT 2 cut(s) 126, 594
CviAII CATG 1 cut(s) 562
DdeI CTNAG 2 cut(s) 714, 957
DpnI GATC 8 cut(s) 83, 173, 194, 227, 360, 417, 506, 962
DpnII GATC 8 cut(s) 81, 171, 192, 225, 358, 415, 504, 960
DraI TTTAAA 1 cut(s) 408
Eam1104I CTCTTC 2 cut(s) 195, 372
EarI CTCTTC 2 cut(s) 195, 372
Ecl136II GAGCTC 2 cut(s) 205, 315
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 3 cut(s) 207, 317, 326
Eco31I GGTCTC 1 cut(s) 7
Eco47I GGWCC 2 cut(s) 718, 752
Eco53kI GAGCTC 2 cut(s) 205, 315
Eco57I CTGAAG 2 cut(s) 360, 780
Eco88I CYCGRG 1 cut(s) 641
EcoICRI GAGCTC 2 cut(s) 205, 315
EcoRII CCWGG 2 cut(s) 461, 507
EcoT14I CCWWGG 1 cut(s) 552
EcoT22I ATGCAT 1 cut(s) 440
EcoT38I GRGCYC 3 cut(s) 207, 317, 326
ErhI CCWWGG 1 cut(s) 552
Esp3I CGTCTC 1 cut(s) 617
FaeI CATG 1 cut(s) 565
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 1 cut(s) 765
FatI CATG 1 cut(s) 561
FbaI TGATCA 1 cut(s) 171
FblI GTMKAC 1 cut(s) 548
Fnu4HI GCNGC 4 cut(s) 274, 277, 636, 777
FokI GGATG 4 cut(s) 311, 401, 512, 549
FriOI GRGCYC 3 cut(s) 207, 317, 326
Fsp4HI GCNGC 4 cut(s) 274, 277, 636, 777
FspBI CTAG 2 cut(s) 89, 216
GlaI GCGC 1 cut(s) 106
GluI GCNGC 4 cut(s) 274, 277, 636, 777
HaeIII GGCC 2 cut(s) 41, 635
HapII CCGG 1 cut(s) 35
HhaI GCGC 1 cut(s) 107
Hin1II CATG 1 cut(s) 565
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HindIII AAGCTT 1 cut(s) 950
HinfI GANTC 5 cut(s) 128, 335, 353, 383, 520
HpaII CCGG 1 cut(s) 35
HphI GGTGA 3 cut(s) 88, 592, 640
Hpy166II GTNNAC 2 cut(s) 549, 787
Hpy188I TCNGA 6 cut(s) 197, 319, 340, 358, 388, 960
Hpy188III TCNNGA 6 cut(s) 25, 89, 362, 571, 643, 659
Hpy8I GTNNAC 2 cut(s) 549, 787
Hpy99I CGWCG 2 cut(s) 119, 233
HpyAV CCTTC 5 cut(s) 286, 463, 482, 625, 761
HpyCH4III ACNGT 3 cut(s) 459, 628, 735
HpyCH4IV ACGT 2 cut(s) 48, 231
HpyCH4V TGCA 2 cut(s) 438, 909
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 321
HpyF3I CTNAG 2 cut(s) 714, 957
HpySE526I ACGT 2 cut(s) 48, 231
Hsp92II CATG 1 cut(s) 565
HspAI GCGC 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 171
KspI CCGCGG 2 cut(s) 277, 535
Kzo9I GATC 8 cut(s) 81, 171, 192, 225, 358, 415, 504, 960
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 3 cut(s) 31, 210, 935
Lsp1109I GCAGC 1 cut(s) 763
LweI GCATC 5 cut(s) 447, 527, 567, 700, 918
MaeI CTAG 2 cut(s) 89, 216
MaeII ACGT 2 cut(s) 48, 231
MaeIII GTNAC 1 cut(s) 76
MalI GATC 8 cut(s) 83, 173, 194, 227, 360, 417, 506, 962
MboI GATC 8 cut(s) 81, 171, 192, 225, 358, 415, 504, 960
MflI RGATCY 2 cut(s) 504, 960
MhlI GDGCHC 3 cut(s) 207, 317, 326
MlyI GAGTC 1 cut(s) 344
Mph1103I ATGCAT 1 cut(s) 440
MseI TTAA 4 cut(s) 53, 407, 726, 737
MspA1I CMGCKG 2 cut(s) 276, 534
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 2 cut(s) 463, 509
Mva1269I GAATGC 2 cut(s) 158, 440
MvaI CCWGG 2 cut(s) 463, 509
MvnI CGCG 3 cut(s) 105, 276, 534
MwoI GCNNNNNNNGC 2 cut(s) 119, 321
NdeII GATC 8 cut(s) 81, 171, 192, 225, 358, 415, 504, 960
NlaIII CATG 1 cut(s) 565
NlaIV GGNNCC 2 cut(s) 33, 753
NmuCI GTSAC 1 cut(s) 76
NsiI ATGCAT 1 cut(s) 440
PaeR7I CTCGAG 1 cut(s) 641
PciSI GCTCTTC 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 2 cut(s) 158, 440
PfeI GAWTC 4 cut(s) 128, 353, 383, 520
PfoI TCCNGGA 1 cut(s) 507
PkrI GCNGC 4 cut(s) 275, 278, 637, 778
Ple19I CGATCG 1 cut(s) 84
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
Psp124BI GAGCTC 2 cut(s) 207, 317
Psp6I CCWGG 2 cut(s) 461, 507
PspGI CCWGG 2 cut(s) 461, 507
PspN4I GGNNCC 2 cut(s) 33, 753
PspPI GGNCC 2 cut(s) 718, 752
PsuI RGATCY 2 cut(s) 504, 960
PvuI CGATCG 1 cut(s) 84
SacI GAGCTC 2 cut(s) 207, 317
SacII CCGCGG 2 cut(s) 277, 535
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 4 cut(s) 53, 407, 726, 737
SatI GCNGC 4 cut(s) 274, 277, 636, 777
Sau3AI GATC 8 cut(s) 81, 171, 192, 225, 358, 415, 504, 960
Sau96I GGNCC 2 cut(s) 718, 752
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 2 cut(s) 463, 509
SduI GDGCHC 3 cut(s) 207, 317, 326
SfaNI GCATC 5 cut(s) 447, 527, 567, 700, 918
Sfr274I CTCGAG 1 cut(s) 641
Sfr303I CCGCGG 2 cut(s) 277, 535
SgrBI CCGCGG 2 cut(s) 277, 535
SinI GGWCC 2 cut(s) 718, 752
SlaI CTCGAG 1 cut(s) 641
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
SsiI CCGC 6 cut(s) 271, 274, 276, 532, 534, 636
SspMI CTAG 2 cut(s) 89, 216
SstI GAGCTC 2 cut(s) 207, 317
StyD4I CCNGG 2 cut(s) 461, 507
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 3 cut(s) 459, 628, 735
TaiI ACGT 2 cut(s) 51, 234
TaqI TCGA 7 cut(s) 117, 126, 228, 255, 441, 594, 642
TaqII GACCGA 1 cut(s) 31
TauI GCSGC 3 cut(s) 276, 279, 638
TfiI GAWTC 4 cut(s) 128, 353, 383, 520
Tru1I TTAA 4 cut(s) 53, 407, 726, 737
Tru9I TTAA 4 cut(s) 53, 407, 726, 737
TscAI CASTG 1 cut(s) 462
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 1 cut(s) 776
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 1 cut(s) 360
TspGWI ACGGA 2 cut(s) 44, 842
TspRI CASTG 1 cut(s) 462
VpaK11BI GGWCC 2 cut(s) 718, 752
XbaI TCTAGA 1 cut(s) 88
XhoI CTCGAG 1 cut(s) 641
XmiI GTMKAC 1 cut(s) 548
XspI CTAG 2 cut(s) 89, 216
Zsp2I ATGCAT 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.