Rw7G006860

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000912
Physical Location & Seq
Forward (+)
5871409 .. 5872937
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G006860.1

Sequence Viewer

Length: 1191 bp
ATGGTAGACAGGGGAGTTAAAGCAGCTATAAGAAACACCAAGATCAGTGCAACCCTGGGCCACAATTGGATGTTCTGTGTACGGATCAAATGGTTGTTACTGCTTTCAGAAATTCCTGAAGTGACAGAGGTACCTAGGTTTAGTAAATCTGCTCAATCTTATTTGCAAGAAATAATTGATGGATTTAACATCGATGATGCATTTGAAGTAAAGAACCTTGAGAAAGTGGCAAACGACGGTGTAGCTGCGGTGGAGCGGTTCATGAAACAGAGATGTGAATCACATCCAGAAATAGCTAAGGTGGTTGGATTTTTTAATTTTGCTTGCACATCTGAGGAGGTCAACAATCTTGCCCATGCACTAATCCTAAAAGGAACCATGGACAATGTCATATTTCCTGCCATGGATGATTTGATTCATGCAATATGTGGCATTGCTAAGAACACTGCTCATATTCCTATTCTCTCTTCCACTCATGGGCAGCCTGTTGCGTTTACAACTTTGGGGAAAGAAATGGCAAGTTTTGCTGTGAGATTAAGCAGAGAACGGAAAGAGATTTCGCAGCTGGAGCTAATGGGGAATCTCGGAGGTGAGGTTGGAAATTACAGTGCTTATCAAGTTGCATATCCTGGTATTAATTGGCCTCAAGTTGCTGAACAGTTTGTAACATCTCTGGGTTTGAGTTTTAATCCCTATGTTACCCGGATCAGAGCTTATGATTATGTGGCAGAATTTGTTCATGGCGTTAACCAGTTCAACAAGATATTGACGGACTTTGATGGAGACGTAGAAGGCTACATGAAGTTACCTATTTCAACTCTTTTGAGGAACATGGGGTCAAGATTAGAGCATTCTCTTGTCACCTACAAAAACACACTCGATCTAATATCAAGACTCGAGGTGGTCGATATAGATTGCCCGATTGAGGACTACTTGAAGCTCTATTCCGGAGCTCTCGTCAAAGAGTTAGAGACGGTAGTGCAAAGATATGGTGTCCCCAAGCCCTATGAAAAATTTAAGGTACTCACCGGAGGACGAGCAGTCGTTACCAAGGAAAGTATAGTGGATTTTATTCAACGCTTGGAAGTACCTAACGAAGCAAAAACCATTCTACTCAAGTTAACACGATGTAGCTACGTCGGAGCCGCTTCGGAATTGGCCAGAACAGTAGACATCGCCGTGAATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

396

Amino Acids

44.2

Weight (kDa)

5.97

Isoelectric Point (pI)

26.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 45 - 271 1.8e-27 Lyase
ASL_C PF08328 272 - 380 9.1e-17 Adenylosuccinate lyase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 130
AccBSI CCGCTC 1 cut(s) 256
AccI GTMKAC 2 cut(s) 6, 1170
AccIII TCCGGA 1 cut(s) 947
AciI CCGC 3 cut(s) 248, 256, 1146
AclWI GGATC 2 cut(s) 92, 713
AcoI YGGCCR 1 cut(s) 1158
AcsI RAATTY 4 cut(s) 111, 731, 1013, 1183
AcuI CTGAAG 1 cut(s) 138
AfaI GTAC 4 cut(s) 81, 132, 1023, 1089
AfiI CCNNNNNNNGG 2 cut(s) 477, 925
AgsI TTSAA 5 cut(s) 206, 757, 816, 937, 1076
AhdI GACNNNNNGTC 1 cut(s) 1040
AjnI CCWGG 2 cut(s) 54, 628
AluBI AGCT 9 cut(s) 26, 245, 296, 565, 571, 713, 940, 953, 1134
AluI AGCT 9 cut(s) 26, 245, 296, 565, 571, 713, 940, 953, 1134
Alw21I GWGCWC 1 cut(s) 955
Alw26I GTCTC 2 cut(s) 777, 965
AlwI GGATC 2 cut(s) 92, 713
Ama87I CYCGRG 1 cut(s) 896
Aor13HI TCCGGA 1 cut(s) 947
AoxI GGCC 3 cut(s) 58, 641, 1158
ApeKI GCWGC 4 cut(s) 23, 245, 481, 562
ApoI RAATTY 4 cut(s) 111, 731, 1013, 1183
AseI ATTAAT 1 cut(s) 636
Asp700I GAANNNNTTC 1 cut(s) 735
Asp718I GGTACC 1 cut(s) 130
AspA2I CCTAGG 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 58
AsuC2I CCSGG 1 cut(s) 703
AsuHPI GGTGA 3 cut(s) 602, 853, 1018
AvaI CYCGRG 1 cut(s) 896
AvrII CCTAGG 1 cut(s) 134
BaeI ACNNNNGTAYC 4 cut(s) 114, 122, 147, 155
BalI TGGCCA 1 cut(s) 1160
BanI GGYRCC 1 cut(s) 130
BanII GRGCYC 1 cut(s) 955
Bbv12I GWGCWC 1 cut(s) 955
BbvI GCAGC 4 cut(s) 35, 232, 493, 574
BccI CCATC 2 cut(s) 173, 773
BceAI ACGGC 1 cut(s) 1163
BciT130I CCWGG 2 cut(s) 56, 630
BcnI CCSGG 1 cut(s) 703
BcoDI GTCTC 2 cut(s) 777, 965
BfaI CTAG 1 cut(s) 135
BisI GCNGC 5 cut(s) 24, 246, 482, 563, 1146
BlnI CCTAGG 1 cut(s) 134
BlsI GCNGC 5 cut(s) 25, 247, 483, 564, 1147
Bme1390I CCNGG 3 cut(s) 56, 630, 703
BmeRI GACNNNNNGTC 1 cut(s) 1040
BmeT110I CYCGRG 1 cut(s) 896
BmgT120I GGNCC 1 cut(s) 58
BmiI GGNNCC 3 cut(s) 132, 376, 1144
BmrFI CCNGG 3 cut(s) 56, 630, 703
BmsI GCATC 1 cut(s) 187
BpmI CTGGAG 1 cut(s) 587
Bpu10I CCTNAGC 1 cut(s) 297
BpuEI CTTGAG 3 cut(s) 239, 630, 1100
BpuMI CCSGG 1 cut(s) 703
Bsa29I ATCGAT 1 cut(s) 192
BsaBI GATNNNNATC 1 cut(s) 277
BsaJI CCNNGG 6 cut(s) 54, 55, 134, 378, 402, 1050
BsaWI WCCGGW 2 cut(s) 947, 1028
BsaXI ACNNNNNCTCC 4 cut(s) 579, 609, 942, 972
Bsc4I CCNNNNNNNGG 2 cut(s) 477, 925
Bse1I ACTGG 1 cut(s) 751
Bse3DI GCAATG 1 cut(s) 432
Bse8I GATNNNNATC 1 cut(s) 277
BseAI TCCGGA 1 cut(s) 947
BseBI CCWGG 2 cut(s) 56, 630
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 6 cut(s) 54, 55, 134, 378, 402, 1050
BseGI GGATG 3 cut(s) 75, 283, 412
BseJI GATNNNNATC 1 cut(s) 277
BseLI CCNNNNNNNGG 2 cut(s) 477, 925
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 751
BseRI GAGGAG 1 cut(s) 350
BseXI GCAGC 4 cut(s) 35, 232, 493, 574
BshFI GGCC 3 cut(s) 60, 643, 1160
BshNI GGYRCC 1 cut(s) 130
BshVI ATCGAT 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 955
BsiHKCI CYCGRG 1 cut(s) 896
BsiSI CCGG 3 cut(s) 703, 948, 1029
BslFI GGGAC 1 cut(s) 980
BslI CCNNNNNNNGG 2 cut(s) 477, 925
BsmAI GTCTC 2 cut(s) 777, 965
BsmBI CGTCTC 2 cut(s) 777, 965
BsmFI GGGAC 1 cut(s) 980
BsmI GAATGC 1 cut(s) 850
BsnI GGCC 3 cut(s) 60, 643, 1160
BsoBI CYCGRG 1 cut(s) 896
Bsp1286I GDGCHC 1 cut(s) 955
Bsp13I TCCGGA 1 cut(s) 947
Bsp143I GATC 4 cut(s) 42, 84, 705, 880
Bsp19I CCATGG 2 cut(s) 378, 402
BspACI CCGC 3 cut(s) 248, 256, 1146
BspANI GGCC 3 cut(s) 60, 643, 1160
BspCNI CTCAG 1 cut(s) 325
BspDI ATCGAT 1 cut(s) 192
BspEI TCCGGA 1 cut(s) 947
BspHI TCATGA 1 cut(s) 261
BspLI GGNNCC 3 cut(s) 132, 376, 1144
BspPI GGATC 2 cut(s) 92, 713
BspT107I GGYRCC 1 cut(s) 130
BsrBI CCGCTC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 432
BsrI ACTGG 1 cut(s) 751
BssECI CCNNGG 6 cut(s) 54, 55, 134, 378, 402, 1050
BssMI GATC 4 cut(s) 42, 84, 705, 880
BssT1I CCWWGG 4 cut(s) 134, 378, 402, 1050
Bst2UI CCWGG 2 cut(s) 56, 630
Bst4CI ACNGT 5 cut(s) 239, 608, 660, 976, 1168
Bst6I CTCTTC 1 cut(s) 472
BstAPI GCANNNNNTGC 1 cut(s) 524
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 3 cut(s) 297, 333, 438
BstDSI CCRYGG 2 cut(s) 378, 402
BstF5I GGATG 3 cut(s) 75, 283, 412
BstKTI GATC 4 cut(s) 45, 87, 708, 883
BstMAI GTCTC 2 cut(s) 777, 965
BstMBI GATC 4 cut(s) 42, 84, 705, 880
BstMWI GCNNNNNNNGC 2 cut(s) 524, 568
BstNI CCWGG 2 cut(s) 56, 630
BstSCI CCNGG 3 cut(s) 54, 628, 701
BstV1I GCAGC 4 cut(s) 35, 232, 493, 574
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 3 cut(s) 60, 643, 1160
BsuTUI ATCGAT 1 cut(s) 192
BtgI CCRYGG 2 cut(s) 378, 402
BtgZI GCGATG 1 cut(s) 1159
BtsCI GGATG 3 cut(s) 75, 283, 412
BtsI GCAGTG 1 cut(s) 444
BtsIMutI CAGTG 3 cut(s) 52, 444, 613
Cac8I GCNNGC 1 cut(s) 325
CciI TCATGA 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 58
ClaI ATCGAT 1 cut(s) 192
Csp6I GTAC 4 cut(s) 80, 131, 1022, 1088
CviAII CATG 9 cut(s) 262, 356, 379, 403, 419, 476, 740, 799, 832
CviQI GTAC 4 cut(s) 80, 131, 1022, 1088
DdeI CTNAG 3 cut(s) 297, 333, 438
DpnI GATC 4 cut(s) 44, 86, 707, 882
DpnII GATC 4 cut(s) 42, 84, 705, 880
DriI GACNNNNNGTC 1 cut(s) 1040
EaeI YGGCCR 1 cut(s) 1158
Eam1104I CTCTTC 1 cut(s) 472
Eam1105I GACNNNNNGTC 1 cut(s) 1040
EarI CTCTTC 1 cut(s) 472
Ecl136II GAGCTC 1 cut(s) 953
Eco130I CCWWGG 4 cut(s) 134, 378, 402, 1050
Eco24I GRGCYC 1 cut(s) 955
Eco53kI GAGCTC 1 cut(s) 953
Eco57I CTGAAG 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 896
EcoICRI GAGCTC 1 cut(s) 953
EcoRI GAATTC 1 cut(s) 1183
EcoRII CCWGG 2 cut(s) 54, 628
EcoT14I CCWWGG 4 cut(s) 134, 378, 402, 1050
EcoT22I ATGCAT 1 cut(s) 202
EcoT38I GRGCYC 1 cut(s) 955
ErhI CCWWGG 4 cut(s) 134, 378, 402, 1050
Esp3I CGTCTC 2 cut(s) 777, 965
FaeI CATG 9 cut(s) 265, 359, 382, 406, 422, 479, 743, 802, 835
FaqI GGGAC 1 cut(s) 980
FatI CATG 9 cut(s) 261, 355, 378, 402, 418, 475, 739, 798, 831
FblI GTMKAC 2 cut(s) 6, 1170
Fnu4HI GCNGC 5 cut(s) 24, 246, 482, 563, 1146
FokI GGATG 3 cut(s) 82, 270, 419
FriOI GRGCYC 1 cut(s) 955
Fsp4HI GCNGC 5 cut(s) 24, 246, 482, 563, 1146
FspBI CTAG 1 cut(s) 135
GluI GCNGC 5 cut(s) 24, 246, 482, 563, 1146
GsuI CTGGAG 1 cut(s) 587
HaeIII GGCC 3 cut(s) 60, 643, 1160
HapII CCGG 3 cut(s) 703, 948, 1029
Hin1II CATG 9 cut(s) 265, 359, 382, 406, 422, 479, 743, 802, 835
HincII GTYRAC 3 cut(s) 343, 748, 1122
HindII GTYRAC 3 cut(s) 343, 748, 1122
HinfI GANTC 4 cut(s) 278, 415, 580, 894
HpaI GTTAAC 2 cut(s) 748, 1122
HpaII CCGG 3 cut(s) 703, 948, 1029
HphI GGTGA 3 cut(s) 602, 853, 1018
Hpy166II GTNNAC 7 cut(s) 7, 80, 343, 495, 748, 1122, 1171
Hpy188I TCNGA 6 cut(s) 109, 334, 587, 710, 1142, 1153
Hpy188III TCNNGA 6 cut(s) 116, 262, 287, 840, 891, 948
Hpy8I GTNNAC 7 cut(s) 7, 80, 343, 495, 748, 1122, 1171
Hpy99I CGWCG 2 cut(s) 239, 1142
HpyAV CCTTC 1 cut(s) 785
HpyCH4III ACNGT 5 cut(s) 239, 608, 660, 976, 1168
HpyCH4IV ACGT 2 cut(s) 786, 1137
HpyCH4V TGCA 8 cut(s) 50, 166, 200, 327, 359, 422, 623, 982
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 568
HpyF3I CTNAG 3 cut(s) 297, 333, 438
HpySE526I ACGT 2 cut(s) 786, 1137
Hsp92II CATG 9 cut(s) 265, 359, 382, 406, 422, 479, 743, 802, 835
Kpn2I TCCGGA 1 cut(s) 947
KpnI GGTACC 1 cut(s) 134
KspAI GTTAAC 2 cut(s) 748, 1122
Kzo9I GATC 4 cut(s) 42, 84, 705, 880
LmnI GCTCC 4 cut(s) 253, 568, 950, 1142
Lsp1109I GCAGC 4 cut(s) 35, 232, 493, 574
LweI GCATC 1 cut(s) 187
MaeI CTAG 1 cut(s) 135
MaeII ACGT 2 cut(s) 786, 1137
MaeIII GTNAC 7 cut(s) 96, 121, 664, 697, 804, 859, 1045
MalI GATC 4 cut(s) 44, 86, 707, 882
MbiI CCGCTC 1 cut(s) 256
MboI GATC 4 cut(s) 42, 84, 705, 880
MboII GAAGA 1 cut(s) 459
MfeI CAATTG 1 cut(s) 64
MhlI GDGCHC 1 cut(s) 955
MlsI TGGCCA 1 cut(s) 1160
MluNI TGGCCA 1 cut(s) 1160
MlyI GAGTC 1 cut(s) 888
MmeI TCCRAC 3 cut(s) 286, 577, 1120
Mox20I TGGCCA 1 cut(s) 1160
Mph1103I ATGCAT 1 cut(s) 202
MroI TCCGGA 1 cut(s) 947
MroXI GAANNNNTTC 1 cut(s) 735
MscI TGGCCA 1 cut(s) 1160
MslI CAYNNNNRTG 1 cut(s) 1178
Msp20I TGGCCA 1 cut(s) 1160
MspA1I CMGCKG 1 cut(s) 565
MspI CCGG 3 cut(s) 703, 948, 1029
MspR9I CCNGG 3 cut(s) 56, 630, 703
MunI CAATTG 1 cut(s) 64
Mva1269I GAATGC 1 cut(s) 850
MvaI CCWGG 2 cut(s) 56, 630
MwoI GCNNNNNNNGC 2 cut(s) 524, 568
NciI CCSGG 1 cut(s) 703
NcoI CCATGG 2 cut(s) 378, 402
NdeII GATC 4 cut(s) 42, 84, 705, 880
NlaIII CATG 9 cut(s) 265, 359, 382, 406, 422, 479, 743, 802, 835
NlaIV GGNNCC 3 cut(s) 132, 376, 1144
NmuCI GTSAC 2 cut(s) 121, 859
NsiI ATGCAT 1 cut(s) 202
PaeR7I CTCGAG 1 cut(s) 896
PagI TCATGA 1 cut(s) 261
PasI CCCWGGG 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 903
PctI GAATGC 1 cut(s) 850
PdmI GAANNNNTTC 1 cut(s) 735
PfeI GAWTC 3 cut(s) 278, 415, 580
PflFI GACNNNGTC 1 cut(s) 386
PkrI GCNGC 5 cut(s) 25, 247, 483, 564, 1147
PleI GAGTC 1 cut(s) 888
PpsI GAGTC 1 cut(s) 888
PshBI ATTAAT 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 955
Psp6I CCWGG 2 cut(s) 54, 628
PspGI CCWGG 2 cut(s) 54, 628
PspN4I GGNNCC 3 cut(s) 132, 376, 1144
PspPI GGNCC 1 cut(s) 58
PspXI VCTCGAGB 1 cut(s) 896
PsyI GACNNNGTC 1 cut(s) 386
PvuII CAGCTG 1 cut(s) 565
RsaI GTAC 4 cut(s) 81, 132, 1023, 1089
RsaNI GTAC 4 cut(s) 80, 131, 1022, 1088
RseI CAYNNNNRTG 1 cut(s) 1178
SacI GAGCTC 1 cut(s) 955
SatI GCNGC 5 cut(s) 24, 246, 482, 563, 1146
Sau3AI GATC 4 cut(s) 42, 84, 705, 880
Sau96I GGNCC 1 cut(s) 58
SchI GAGTC 1 cut(s) 888
ScrFI CCNGG 3 cut(s) 56, 630, 703
SduI GDGCHC 1 cut(s) 955
SfaNI GCATC 1 cut(s) 187
Sfr274I CTCGAG 1 cut(s) 896
SlaI CTCGAG 1 cut(s) 896
SmiMI CAYNNNNRTG 1 cut(s) 1178
SmlI CTYRAG 4 cut(s) 218, 645, 896, 1115
SmoI CTYRAG 4 cut(s) 218, 645, 896, 1115
SsiI CCGC 3 cut(s) 248, 256, 1146
SspMI CTAG 1 cut(s) 135
SstI GAGCTC 1 cut(s) 955
StyD4I CCNGG 3 cut(s) 54, 628, 701
StyI CCWWGG 4 cut(s) 134, 378, 402, 1050
TaaI ACNGT 5 cut(s) 239, 608, 660, 976, 1168
TaiI ACGT 2 cut(s) 789, 1140
TaqI TCGA 4 cut(s) 192, 879, 897, 906
TauI GCSGC 1 cut(s) 1148
TfiI GAWTC 3 cut(s) 278, 415, 580
TscAI CASTG 3 cut(s) 52, 451, 613
TseFI GTSAC 2 cut(s) 121, 859
TseI GCWGC 4 cut(s) 23, 245, 481, 562
Tsp45I GTSAC 2 cut(s) 121, 859
TspDTI ATGAA 6 cut(s) 250, 278, 407, 728, 815, 1023
TspGWI ACGGA 3 cut(s) 97, 562, 785
TspRI CASTG 3 cut(s) 52, 451, 613
Tth111I GACNNNGTC 1 cut(s) 386
VspI ATTAAT 1 cut(s) 636
XapI RAATTY 4 cut(s) 111, 731, 1013, 1183
XhoI CTCGAG 1 cut(s) 896
XmaJI CCTAGG 1 cut(s) 134
XmiI GTMKAC 2 cut(s) 6, 1170
XmnI GAANNNNTTC 1 cut(s) 735
XspI CTAG 1 cut(s) 135
Zsp2I ATGCAT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.