AT4G18440

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000912
Physical Location & Seq
Reverse (-)
10186073 .. 10188981
2909 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18440.1

Sequence Viewer

Length: 1611 bp
ATGGAGCTCAATTTCTCTTCAATGGCTATAACACACCCCAAAATCCCTTCTTTCGGTTTTACACCCACCGGAATATTCCTAAACCCATCAAAATCAGTGTGTTTGGCTTCACATCATCGGCTTCCGAGAGTTTCATGCTCTGTTTCTACTACTACTGATTCTCCCAAGCTAGTGACTTCAACAAAGGTGACGGCAATGGATGGAGTGAGCTCTAGAGACTTGGAGATGTCGAATTTAACGGCGTTATCGCCTTTGGATGGACGTTATTGGAGTAAAGTTAAGGACTTGGCTTCTTCTTTGAGCGAGTTTGGATTGATCTATTTCCGAGTTTTTGTCGAGATCAAATGGCTTCTTAAGCTTTCGAATATTCCTGAAGTCACTGAAGTTCCAAGCTTTAGCAAAGAAGCTCAGAGTTTCTTGCAAGGCATAATCGATGGATTTAGCATAGACGATGCATTGGAAATTAAGAAGATTGAGAGAGTAACAAATCATGATGTGAAAGCAGTGGAGTATTTCTTGAAACAAAAGTGTGAATCACAACCAGAGATTGCTAAGGTTCTTGAGTTTTTCCATTTCGCTTGCACGTCTGAGGACATCAACAATCTTTCCCATGCTTTGATGCTTCAAGAAGCACTTAGTTCGGTTATACTTCCTACCATGGATGAGCTGATCAAGTCAATCTCTCTGATAGCTAAGAACTTTGCTTATGTCCCCATGCTTTCACGAACTCATGGGCAGCCAGCTACGCCAACAACTTTGGGGAAAGAAATGGCGAATTTTGCTGTGAGGTTAAGCGAAGAAAGGAGATATCTTTCAGAAACTAAGATTAAGGGGAAATTTGCAGGTGCTGTTGGGAACTACAACGCCCATATTTCCGCATATTCGAATATTGACTGGCCTCATGTTTCCGAGGAGTTTGTTACTTCTCTTGGATTAACATTCAACCCATACGTGACTCAGATTGAACCTCATGACTATATGGCTAGACTTTTTAATAATATCAGCCAGTTCAACACTATTTTAATTGATTTTGACAGAGATATATGGAGCTACATATCTCTAGGGTACTTTAAGCAGACAACTAAAGCGGGTGAAATTGGATCGTCGACAATGCCTCACAAAGTGAATCCTATTGACTTTGAGAACAGCGAAGGGAATCTAGGGAAAGCAAACGCAGAGCTTACTTTTCTCAGCATGAAGCTTCCCATTTCACGCATGCAGCGTGATTTAACTGATTCAACTGTCTTGAGAAACATGGGTGGAGCTTTAGGACACTCTCTTCTCGCTTACAAGAGTGCGATACAGGGAATCGGGAAGCTTCAGGTTAATGAAGCTCGGTTAAAAGAAGATTTGGATGATAATTGGGAAGTCCTTGCTGAACCAATACAAACTGTGATGAGGAGATACGGTGTCCCTGAGCCGTATGAGAAGCTGAAGGAGCTAACAAGAGGAAAAGCTGTGAATGAAGAAACCATAAGAACGTTTATCAAAGGTTTGGAATTGCCTTCAGAAGCAAAAGACCAACTTCTGGAGCTAACTCCACACACATATGTTGGTGCTGCTGCTGCATTGGCACTGGCCGTGGATGAAGCTCTGCACTTGGGACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

536

Amino Acids

59.76

Weight (kDa)

5.89

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 130 - 388 8.8e-57 Lyase
ASL_C PF08328 404 - 518 1.8e-51 Adenylosuccinate lyase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 833
Acc36I ACCTGC 1 cut(s) 833
AccB7I CCANNNNNTGG 1 cut(s) 1528
AccI GTMKAC 1 cut(s) 1106
AciI CCGC 2 cut(s) 876, 1088
AclI AACGTT 1 cut(s) 1481
AclWI GGATC 1 cut(s) 1108
AcoI YGGCCR 1 cut(s) 1578
AcsI RAATTY 3 cut(s) 232, 775, 836
AcuI CTGAAG 5 cut(s) 393, 402, 1304, 1454, 1491
AdeI CACNNNGTG 1 cut(s) 1123
AfaI GTAC 1 cut(s) 1067
AfiI CCNNNNNNNGG 3 cut(s) 53, 257, 1528
AflII CTTAAG 1 cut(s) 353
AgsI TTSAA 8 cut(s) 21, 180, 520, 626, 943, 965, 1012, 1239
AjiI CACGTC 1 cut(s) 585
Alw21I GWGCWC 2 cut(s) 9, 212
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 1 cut(s) 1108
AlwNI CAGNNNCTG 1 cut(s) 848
AoxI GGCC 2 cut(s) 896, 1578
ApeKI GCWGC 5 cut(s) 736, 1219, 1559, 1562, 1565
ApoI RAATTY 3 cut(s) 232, 775, 836
AsuHPI GGTGA 2 cut(s) 199, 1103
AsuII TTCGAA 2 cut(s) 362, 884
BanII GRGCYC 2 cut(s) 9, 212
Bbv12I GWGCWC 2 cut(s) 9, 212
BbvI GCAGC 5 cut(s) 748, 1231, 1546, 1549, 1552
BccI CCATC 4 cut(s) 94, 194, 251, 428
BceAI ACGGC 4 cut(s) 207, 255, 1405, 1565
BcgI CGANNNNNNTGC 2 cut(s) 621, 655
BclI TGATCA 1 cut(s) 669
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 5 cut(s) 170, 213, 984, 1061, 1160
BfrI CTTAAG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 833
BisI GCNGC 5 cut(s) 737, 1220, 1560, 1563, 1566
BlsI GCNGC 5 cut(s) 738, 1221, 1561, 1564, 1567
BmgBI CACGTC 1 cut(s) 585
BmsI GCATC 2 cut(s) 442, 609
BpmI CTGGAG 1 cut(s) 1550
Bpu10I CCTNAGC 2 cut(s) 552, 1416
Bpu14I TTCGAA 2 cut(s) 362, 884
BpuEI CTTGAG 2 cut(s) 581, 1267
Bsa29I ATCGAT 1 cut(s) 432
BsaAI YACGTR 1 cut(s) 952
BsaJI CCNNGG 3 cut(s) 657, 909, 1581
BsaWI WCCGGW 1 cut(s) 68
BsaXI ACNNNNNCTCC 4 cut(s) 145, 175, 1393, 1423
Bsc4I CCNNNNNNNGG 3 cut(s) 53, 257, 1528
Bse1I ACTGG 3 cut(s) 899, 1006, 1581
Bse3DI GCAATG 1 cut(s) 201
BseCI ATCGAT 1 cut(s) 432
BseDI CCNNGG 3 cut(s) 657, 909, 1581
BseGI GGATG 5 cut(s) 205, 262, 667, 1360, 1591
BseLI CCNNNNNNNGG 3 cut(s) 53, 257, 1528
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 5 cut(s) 422, 579, 971, 1204, 1407
BseNI ACTGG 3 cut(s) 899, 1006, 1581
BseRI GAGGAG 2 cut(s) 926, 1414
BseXI GCAGC 5 cut(s) 748, 1231, 1546, 1549, 1552
BsgI GTGCAG 1 cut(s) 1580
BshFI GGCC 2 cut(s) 898, 1580
BshVI ATCGAT 1 cut(s) 432
BsiHKAI GWGCWC 2 cut(s) 9, 212
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 2 cut(s) 695, 1397
BslI CCNNNNNNNGG 3 cut(s) 53, 257, 1528
BsmAI GTCTC 1 cut(s) 210
BsmFI GGGAC 2 cut(s) 695, 1397
BsnI GGCC 2 cut(s) 898, 1580
Bsp119I TTCGAA 2 cut(s) 362, 884
Bsp1286I GDGCHC 2 cut(s) 9, 212
Bsp143I GATC 4 cut(s) 315, 339, 669, 1100
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 2 cut(s) 876, 1088
BspANI GGCC 2 cut(s) 898, 1580
BspCNI CTCAG 5 cut(s) 421, 580, 970, 1203, 1408
BspDI ATCGAT 1 cut(s) 432
BspHI TCATGA 2 cut(s) 490, 970
BspMI ACCTGC 1 cut(s) 833
BspPI GGATC 1 cut(s) 1108
BspT104I TTCGAA 2 cut(s) 362, 884
BspTI CTTAAG 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 201
BsrI ACTGG 3 cut(s) 899, 1006, 1581
BssECI CCNNGG 3 cut(s) 657, 909, 1581
BssMI GATC 4 cut(s) 315, 339, 669, 1100
BssT1I CCWWGG 1 cut(s) 657
Bst4CI ACNGT 3 cut(s) 1243, 1393, 1409
Bst6I CTCTTC 2 cut(s) 22, 1284
BstAFI CTTAAG 1 cut(s) 353
BstBAI YACGTR 1 cut(s) 952
BstBI TTCGAA 2 cut(s) 362, 884
BstC8I GCNNGC 3 cut(s) 580, 741, 1217
BstDEI CTNAG 9 cut(s) 408, 552, 588, 635, 693, 822, 957, 1190, 1416
BstDSI CCRYGG 2 cut(s) 657, 1581
BstF5I GGATG 5 cut(s) 205, 262, 667, 1360, 1591
BstKTI GATC 4 cut(s) 318, 342, 672, 1103
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 4 cut(s) 315, 339, 669, 1100
BstMWI GCNNNNNNNGC 6 cut(s) 355, 745, 779, 1438, 1565, 1571
BstNSI RCATGY 1 cut(s) 1219
BstV1I GCAGC 5 cut(s) 748, 1231, 1546, 1549, 1552
Bsu15I ATCGAT 1 cut(s) 432
BsuRI GGCC 2 cut(s) 898, 1580
BsuTUI ATCGAT 1 cut(s) 432
BtgI CCRYGG 2 cut(s) 657, 1581
BtrI CACGTC 1 cut(s) 585
BtsCI GGATG 5 cut(s) 205, 262, 667, 1360, 1591
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 4 cut(s) 102, 378, 510, 1574
BveI ACCTGC 1 cut(s) 833
Cac8I GCNNGC 3 cut(s) 580, 741, 1217
CaiI CAGNNNCTG 1 cut(s) 848
CciI TCATGA 2 cut(s) 490, 970
ClaI ATCGAT 1 cut(s) 432
Csp6I GTAC 1 cut(s) 1066
CviQI GTAC 1 cut(s) 1066
DdeI CTNAG 9 cut(s) 408, 552, 588, 635, 693, 822, 957, 1190, 1416
DpnI GATC 4 cut(s) 317, 341, 671, 1102
DpnII GATC 4 cut(s) 315, 339, 669, 1100
DraIII CACNNNGTG 1 cut(s) 1123
EaeI YGGCCR 1 cut(s) 1578
Eam1104I CTCTTC 2 cut(s) 22, 1284
EarI CTCTTC 2 cut(s) 22, 1284
Ecl136II GAGCTC 2 cut(s) 7, 210
Eco130I CCWWGG 1 cut(s) 657
Eco24I GRGCYC 2 cut(s) 9, 212
Eco32I GATATC 1 cut(s) 809
Eco53kI GAGCTC 2 cut(s) 7, 210
Eco57I CTGAAG 5 cut(s) 393, 402, 1304, 1454, 1491
EcoICRI GAGCTC 2 cut(s) 7, 210
EcoRV GATATC 1 cut(s) 809
EcoT14I CCWWGG 1 cut(s) 657
EcoT22I ATGCAT 1 cut(s) 457
EcoT38I GRGCYC 2 cut(s) 9, 212
ErhI CCWWGG 1 cut(s) 657
FalI AAGNNNNNCTT 4 cut(s) 618, 650, 1509, 1541
FaqI GGGAC 2 cut(s) 695, 1397
FauI CCCGC 1 cut(s) 1081
FauNDI CATATG 1 cut(s) 1549
FbaI TGATCA 1 cut(s) 669
FblI GTMKAC 1 cut(s) 1106
Fnu4HI GCNGC 5 cut(s) 737, 1220, 1560, 1563, 1566
FokI GGATG 5 cut(s) 212, 269, 674, 1367, 1598
FriOI GRGCYC 2 cut(s) 9, 212
Fsp4HI GCNGC 5 cut(s) 737, 1220, 1560, 1563, 1566
FspBI CTAG 5 cut(s) 170, 213, 984, 1061, 1160
GluI GCNGC 5 cut(s) 737, 1220, 1560, 1563, 1566
GsuI CTGGAG 1 cut(s) 1550
HaeIII GGCC 2 cut(s) 898, 1580
HapII CCGG 1 cut(s) 69
HincII GTYRAC 1 cut(s) 1107
HindII GTYRAC 1 cut(s) 1107
HindIII AAGCTT 4 cut(s) 356, 391, 1199, 1316
HinfI GANTC 7 cut(s) 158, 533, 955, 1126, 1156, 1235, 1308
HpaII CCGG 1 cut(s) 69
HphI GGTGA 2 cut(s) 199, 1103
Hpy166II GTNNAC 1 cut(s) 1107
Hpy188I TCNGA 9 cut(s) 126, 326, 411, 589, 687, 817, 910, 960, 1510
Hpy8I GTNNAC 1 cut(s) 1107
Hpy99I CGWCG 1 cut(s) 1108
HpyAV CCTTC 4 cut(s) 57, 1145, 1429, 1515
HpyCH4III ACNGT 3 cut(s) 1243, 1393, 1409
HpyCH4IV ACGT 4 cut(s) 262, 584, 951, 1481
HpyCH4V TGCA 7 cut(s) 421, 455, 582, 842, 1219, 1568, 1597
HpyF10VI GCNNNNNNNGC 6 cut(s) 355, 745, 779, 1438, 1565, 1571
HpyF3I CTNAG 9 cut(s) 408, 552, 588, 635, 693, 822, 957, 1190, 1416
HpySE526I ACGT 4 cut(s) 262, 584, 951, 1481
Ksp22I TGATCA 1 cut(s) 669
Kzo9I GATC 4 cut(s) 315, 339, 669, 1100
LmnI GCTCC 5 cut(s) 4, 1047, 1262, 1438, 1531
Lsp1109I GCAGC 5 cut(s) 748, 1231, 1546, 1549, 1552
LweI GCATC 2 cut(s) 442, 609
MaeI CTAG 5 cut(s) 170, 213, 984, 1061, 1160
MaeII ACGT 4 cut(s) 262, 584, 951, 1481
MaeIII GTNAC 6 cut(s) 172, 187, 376, 481, 919, 952
MalI GATC 4 cut(s) 317, 341, 671, 1102
MboI GATC 4 cut(s) 315, 339, 669, 1100
MboII GAAGA 7 cut(s) 9, 285, 481, 809, 1271, 1358, 1478
MhlI GDGCHC 2 cut(s) 9, 212
MluCI AATT 9 cut(s) 10, 232, 462, 775, 836, 1023, 1095, 1360, 1499
MlyI GAGTC 1 cut(s) 949
MnlI CCTC 8 cut(s) 583, 780, 904, 909, 978, 1125, 1392, 1442
Mph1103I ATGCAT 1 cut(s) 457
MslI CAYNNNNRTG 1 cut(s) 1548
MspCI CTTAAG 1 cut(s) 353
MspI CCGG 1 cut(s) 69
MwoI GCNNNNNNNGC 6 cut(s) 355, 745, 779, 1438, 1565, 1571
NcoI CCATGG 1 cut(s) 657
NdeI CATATG 1 cut(s) 1549
NdeII GATC 4 cut(s) 315, 339, 669, 1100
NmuCI GTSAC 4 cut(s) 172, 187, 376, 952
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 1 cut(s) 1219
NspV TTCGAA 2 cut(s) 362, 884
PaeI GCATGC 1 cut(s) 1219
PagI TCATGA 2 cut(s) 490, 970
PaqCI CACCTGC 1 cut(s) 833
PcsI WCGNNNNNNNCGW 1 cut(s) 1219
PfeI GAWTC 6 cut(s) 158, 533, 1126, 1156, 1235, 1308
PflMI CCANNNNNTGG 1 cut(s) 1528
PkrI GCNGC 5 cut(s) 738, 1221, 1561, 1564, 1567
PleI GAGTC 1 cut(s) 949
PpsI GAGTC 1 cut(s) 949
Ppu21I YACGTR 1 cut(s) 952
Psp124BI GAGCTC 2 cut(s) 9, 212
Psp1406I AACGTT 1 cut(s) 1481
PstNI CAGNNNCTG 1 cut(s) 848
RsaI GTAC 1 cut(s) 1067
RsaNI GTAC 1 cut(s) 1066
RseI CAYNNNNRTG 1 cut(s) 1548
SacI GAGCTC 2 cut(s) 9, 212
SalI GTCGAC 1 cut(s) 1105
SatI GCNGC 5 cut(s) 737, 1220, 1560, 1563, 1566
Sau3AI GATC 4 cut(s) 315, 339, 669, 1100
SchI GAGTC 1 cut(s) 949
SduI GDGCHC 2 cut(s) 9, 212
SfaNI GCATC 2 cut(s) 442, 609
SfuI TTCGAA 2 cut(s) 362, 884
SmiMI CAYNNNNRTG 1 cut(s) 1548
SmlI CTYRAG 3 cut(s) 353, 560, 1246
SmoI CTYRAG 3 cut(s) 353, 560, 1246
SphI GCATGC 1 cut(s) 1219
Sse9I AATT 9 cut(s) 10, 232, 462, 775, 836, 1023, 1095, 1360, 1499
SsiI CCGC 2 cut(s) 876, 1088
SspI AATATT 3 cut(s) 75, 367, 889
SspMI CTAG 5 cut(s) 170, 213, 984, 1061, 1160
SstI GAGCTC 2 cut(s) 9, 212
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 3 cut(s) 1243, 1393, 1409
TaiI ACGT 4 cut(s) 265, 587, 954, 1484
TaqI TCGA 6 cut(s) 230, 336, 362, 432, 884, 1106
TasI AATT 9 cut(s) 10, 232, 462, 775, 836, 1023, 1095, 1360, 1499
TfiI GAWTC 6 cut(s) 158, 533, 1126, 1156, 1235, 1308
TscAI CASTG 4 cut(s) 102, 385, 510, 1581
TseFI GTSAC 4 cut(s) 172, 187, 376, 952
TseI GCWGC 5 cut(s) 736, 1219, 1559, 1562, 1565
Tsp45I GTSAC 4 cut(s) 172, 187, 376, 952
TspDTI ATGAA 5 cut(s) 123, 1211, 1344, 1479, 1602
TspRI CASTG 4 cut(s) 102, 385, 510, 1581
Van91I CCANNNNNTGG 1 cut(s) 1528
Vha464I CTTAAG 1 cut(s) 353
XapI RAATTY 3 cut(s) 232, 775, 836
XbaI TCTAGA 1 cut(s) 212
XceI RCATGY 1 cut(s) 1219
XmiI GTMKAC 1 cut(s) 1106
XspI CTAG 5 cut(s) 170, 213, 984, 1061, 1160
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.