FvH4_7g08523

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000912
Physical Location & Seq
Reverse (-)
8366769 .. 8369673
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08523.t1

Sequence Viewer

Length: 2181 bp
ATGAGCTCCACTTCAATCCTCCTGGACTTCAACTCCTTCCGATTCACGCCTCTCCCTCCCAAATCCCACAAACCCATCGACCCTTCAACCCTCAATCTTCACAATGCTTCTTTCCCACTTTCTCGCAGAGCTTCCCCTCTCACCTCCGTCTCCGCCGCCGCCAAAGTCGCCGCCTTTCCCGGCGAAACCGAGCATTCCGACTTGACGGCGCTGTCTCCGCTCGACGGCCGTTACTGGGGTAAAGTCAACGAATTGGGTCCTTATTTGAGCGAGTATGGCCTCATCTACTACCGTGTTCTTGTTGAGCGAAGAAGCTCAGGCTTACTTGCAGGGATCATTGATGGGTTTGGCATTAATGATGCAAGAGAGATTAAGAGGATTGAGATGACTACTAACCATGATGTGAAAGCAGTGGAGTATTTCTTGAAGCAGAGGAGTAGTTCACATCCTGAAATAGCTAAGGTGCTTGAGTTTTATCATTTTGCTTGCACTTCTGAGGACATCAACAATATTGCACACGCGTTGATGTTGAAAGAATCTGTTAACAATGTTCTGCTTCCTGTGTTGGATGATTTGATTCGAGGGATATGTGACATGGCAAAGGAGCATGCTGATGTTCCCATGCTTTTGCATACTCATGGACAGACTGCCTCACCCACGACTTTGGGGAAGGAAATGGCTGTTTTCGCTGTCAAGTTAAGCACACAGAGGCAGCAACTTTCTCAAGTGGCAATACTAGGGAAGCTTGCTGGTACTGTAGGAAATTACAATGCTCATATGGTTGCATATCCTAATATTGATTGGCCCTTAGTTTCTCAAGAGTTTGTCACATCTCTTGGGGTTAGTTTCAATCCATATGTTACCCAGATCGAGACACATGACTATATGGCAAAACTTTTTCATGCATATACCAGATTCAATAATATCTTGATCGACTTTGATTGTGATATATGGCGCTACATATCTTTGGGCTACTTTAAGCAGACAACTAAAGCTGGTGAAATTGGGTCGTCAACAATGCCTCACAAACGTGACTTGACTGATTCTACTGTTCTAAGGAACATGGGTATTGGATTAGGCCGTTCTCTTCTGGCCTACAGAAGCACGCTGCAGGGAATATCCAAGCTTCAGGTTAATGAAGCTCGCATAAGTGAAGATTTAAATCACTCTTGGGAGGTGCTTGCAGAACCAATACAAACTGTTATGCGAAGATATGATGTTCCAGAGCCATATGAGAAGTTAAAAGTACCTACAAGAGGGAGAACAGTCACTGCAGAAAGTATAAAAGCGTTTATCAAAGGCTTGGAATTACCAGAAGAACCAAAGAACATTCTCTCAAACTTGACACCACATAGCTATGTTGGATCTGCTGTTATATTGGCCAAGATGGTAGATATGGCCGTTGGAGCCACCATACGAGACACCAATGTTCCTGCTGAGAAGGTGAAGGTTGTGGCTAATAGTTCTTCTTGTGATCTTGAGCTTTCAAGTTTGCTGGCTTTGTCGCCTTTGGATGGCCGGTATTGGGGTAAAGTGAAGGAATTGGCTCCTTATATGAGTGAATATGGTTTAATCTACTTCCGTGTTATAGTTGAGATCAAATGGCTGATATGGCTTTCACAAATTCCTGAAGTGATAGAGGTAACCGGCTTGGGTGAAACTGCTCATTCATTTTTGCAAGAAATAATCGATGGGTTTAGCATAGTTGACGCACTAGAGATTAAGGAGATTGAGAAAGTGACAAACCATGACGTAAAAGCGGTGGAGTATTTTTTGAAACAGAGATGCAAATATCACCCAGAGATAGCAAAGGTGCTTGAGTTTTTTCATTTTGCATGCACATCCGAGGACATCAACAATCTTGCACATGCATTGATGCTCAAGGGAGCTTTGAACAATGTCATGTTTCCTGTCATTGCTAAATTGATTCAGGCACTATGTAGCATGGCTAAGGAGTACTCTCATATTCCTATGTTGTCTCGCACTCATGGACAGCCCGCTTCACCTACGACTTTGGGGAAAGAAATGGCTATATTTGCTGTCAGATTGAGCAGAGAATGGAAAGAGATATCTGGTGTGGATATAATGGGGAAATTGGCTGGTGCTGTTGGAAATTACAATGCTCATGAAGTTGCATATCCTGATGTTAATTGGCCCCAAGTTAACAAAGAGTTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

727

Amino Acids

81.04

Weight (kDa)

6.33

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 112 - 353 4.1e-47 Lyase
ASL_C PF08328 343 - 454 1.5e-43 Adenylosuccinate lyase C-terminal
Lyase_1 PF00206 535 - 716 6.2e-29 Lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 211
AccBSI CCGCTC 1 cut(s) 220
AccII CGCG 1 cut(s) 521
AciI CCGC 7 cut(s) 153, 156, 159, 171, 218, 1760, 1998
AclWI GGATC 2 cut(s) 341, 1372
AcoI YGGCCR 4 cut(s) 226, 1380, 1398, 1516
AcsI RAATTY 1 cut(s) 1623
AcuI CTGAAG 2 cut(s) 1112, 1650
AfaI GTAC 3 cut(s) 754, 1248, 1958
AfiI CCNNNNNNNGG 5 cut(s) 224, 235, 1256, 1514, 1525
AflIII ACRYGT 1 cut(s) 519
AjnI CCWGG 1 cut(s) 21
AleI CACNNNNGTG 1 cut(s) 1029
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 5 cut(s) 154, 219, 866, 1413, 1983
AlwI GGATC 2 cut(s) 341, 1372
AlwNI CAGNNNCTG 1 cut(s) 1271
AoxI GGCC 9 cut(s) 226, 277, 803, 1078, 1092, 1380, 1398, 1516, 2153
ApeKI GCWGC 2 cut(s) 712, 1108
ApoI RAATTY 1 cut(s) 1623
AseI ATTAAT 1 cut(s) 354
AspLEI GCGC 2 cut(s) 211, 957
AspS9I GGNCC 3 cut(s) 257, 804, 2154
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 7 cut(s) 133, 645, 1010, 1456, 1667, 1787, 1995
AvaII GGWCC 1 cut(s) 257
BalI TGGCCA 1 cut(s) 1382
BanII GRGCYC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 2 cut(s) 724, 1095
BccI CCATC 5 cut(s) 83, 335, 1381, 1508, 1685
BceAI ACGGC 5 cut(s) 213, 222, 241, 1065, 1385
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 5 cut(s) 154, 219, 866, 1413, 1983
BfaI CTAG 2 cut(s) 737, 1715
BfmI CTRYAG 4 cut(s) 756, 1096, 1109, 1272
BfoI RGCGCY 2 cut(s) 212, 958
BisI GCNGC 5 cut(s) 156, 159, 171, 713, 1109
BlsI GCNGC 5 cut(s) 157, 160, 172, 714, 1110
BmcAI AGTACT 1 cut(s) 1958
Bme1390I CCNGG 2 cut(s) 23, 180
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 3 cut(s) 257, 804, 2154
BmiI GGNNCC 4 cut(s) 258, 1408, 1548, 2156
BmrFI CCNGG 2 cut(s) 23, 180
BmrI ACTGGG 1 cut(s) 244
BmsI GCATC 3 cut(s) 349, 1775, 1866
BmuI ACTGGG 1 cut(s) 244
Bpu10I CCTNAGC 3 cut(s) 316, 459, 1950
BpuEI CTTGAG 6 cut(s) 488, 708, 801, 1499, 1838, 1865
BpuMI CCSGG 1 cut(s) 180
Bsa29I ATCGAT 1 cut(s) 1689
BsaBI GATNNNNATC 1 cut(s) 1161
BsaJI CCNNGG 1 cut(s) 1845
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 5 cut(s) 224, 235, 1256, 1514, 1525
Bse118I RCCGGY 2 cut(s) 1518, 1646
Bse1I ACTGG 1 cut(s) 239
Bse3DI GCAATG 1 cut(s) 1914
Bse8I GATNNNNATC 1 cut(s) 1161
BseBI CCWGG 1 cut(s) 23
BseCI ATCGAT 1 cut(s) 1689
BseDI CCNNGG 1 cut(s) 1845
BseGI GGATG 4 cut(s) 445, 574, 1519, 1841
BseJI GATNNNNATC 1 cut(s) 1161
BseLI CCNNNNNNNGG 5 cut(s) 224, 235, 1256, 1514, 1525
BseMI GCAATG 1 cut(s) 1914
BseMII CTCAG 3 cut(s) 330, 486, 1428
BseNI ACTGG 1 cut(s) 239
BseRI GAGGAG 1 cut(s) 448
BseX3I CGGCCG 1 cut(s) 226
BseXI GCAGC 2 cut(s) 724, 1095
Bsh1236I CGCG 1 cut(s) 521
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 9 cut(s) 228, 279, 805, 1080, 1094, 1382, 1400, 1518, 2155
BshVI ATCGAT 1 cut(s) 1689
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 3 cut(s) 180, 1519, 1647
BslI CCNNNNNNNGG 5 cut(s) 224, 235, 1256, 1514, 1525
BsmAI GTCTC 5 cut(s) 154, 219, 866, 1413, 1983
BsmBI CGTCTC 1 cut(s) 154
BsmI GAATGC 1 cut(s) 193
BsnI GGCC 9 cut(s) 228, 279, 805, 1080, 1094, 1382, 1400, 1518, 2155
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 6 cut(s) 333, 867, 930, 1364, 1474, 1596
BspACI CCGC 7 cut(s) 153, 156, 159, 171, 218, 1760, 1998
BspANI GGCC 9 cut(s) 228, 279, 805, 1080, 1094, 1382, 1400, 1518, 2155
BspCNI CTCAG 3 cut(s) 329, 487, 1429
BspDI ATCGAT 1 cut(s) 1689
BspFNI CGCG 1 cut(s) 521
BspHI TCATGA 1 cut(s) 2125
BspLI GGNNCC 4 cut(s) 258, 1408, 1548, 2156
BspMAI CTGCAG 2 cut(s) 1113, 1276
BspPI GGATC 2 cut(s) 341, 1372
BsrBI CCGCTC 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 1914
BsrFI RCCGGY 2 cut(s) 1518, 1646
BsrI ACTGG 1 cut(s) 239
BssAI RCCGGY 2 cut(s) 1518, 1646
BssECI CCNNGG 1 cut(s) 1845
BssMI GATC 6 cut(s) 333, 867, 930, 1364, 1474, 1596
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 5 cut(s) 293, 757, 1051, 1201, 1267
Bst6I CTCTTC 1 cut(s) 1092
BstC8I GCNNGC 9 cut(s) 487, 609, 747, 1106, 1144, 1182, 1497, 1837, 1998
BstDEI CTNAG 7 cut(s) 316, 459, 495, 808, 1055, 1437, 1950
BstEII GGTNACC 1 cut(s) 1642
BstENI CCTNNNNNAGG 1 cut(s) 1254
BstF5I GGATG 4 cut(s) 445, 574, 1519, 1841
BstFNI CGCG 1 cut(s) 521
BstH2I RGCGCY 2 cut(s) 212, 958
BstHHI GCGC 2 cut(s) 211, 957
BstKTI GATC 6 cut(s) 336, 870, 933, 1367, 1477, 1599
BstMAI GTCTC 5 cut(s) 154, 219, 866, 1413, 1983
BstMBI GATC 6 cut(s) 333, 867, 930, 1364, 1474, 1596
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 7 cut(s) 167, 217, 276, 686, 1406, 1612, 2036
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 3 cut(s) 611, 1839, 1871
BstPI GGTNACC 1 cut(s) 1642
BstSCI CCNGG 2 cut(s) 21, 178
BstSFI CTRYAG 4 cut(s) 756, 1096, 1109, 1272
BstUI CGCG 1 cut(s) 521
BstV1I GCAGC 2 cut(s) 724, 1095
BstX2I RGATCY 1 cut(s) 1364
BstXI CCANNNNNNTGG 1 cut(s) 664
BstYI RGATCY 1 cut(s) 1364
BstZI CGGCCG 1 cut(s) 226
Bsu15I ATCGAT 1 cut(s) 1689
BsuRI GGCC 9 cut(s) 228, 279, 805, 1080, 1094, 1382, 1400, 1518, 2155
BsuTUI ATCGAT 1 cut(s) 1689
BtsCI GGATG 4 cut(s) 445, 574, 1519, 1841
BtsI GCAGTG 2 cut(s) 417, 1269
BtsIMutI CAGTG 2 cut(s) 417, 1269
Cac8I GCNNGC 9 cut(s) 487, 609, 747, 1106, 1144, 1182, 1497, 1837, 1998
CaiI CAGNNNCTG 1 cut(s) 1271
CciI TCATGA 1 cut(s) 2125
CfoI GCGC 2 cut(s) 211, 957
Cfr10I RCCGGY 2 cut(s) 1518, 1646
Cfr13I GGNCC 3 cut(s) 257, 804, 2154
ClaI ATCGAT 1 cut(s) 1689
CseI GACGC 1 cut(s) 1718
Csp6I GTAC 3 cut(s) 753, 1247, 1957
CviQI GTAC 3 cut(s) 753, 1247, 1957
DdeI CTNAG 7 cut(s) 316, 459, 495, 808, 1055, 1437, 1950
DpnI GATC 6 cut(s) 335, 869, 932, 1366, 1476, 1598
DpnII GATC 6 cut(s) 333, 867, 930, 1364, 1474, 1596
DraI TTTAAA 1 cut(s) 1161
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
EaeI YGGCCR 4 cut(s) 226, 1380, 1398, 1516
EagI CGGCCG 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 1092
EarI CTCTTC 1 cut(s) 1092
EciI GGCGGA 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 6
EclXI CGGCCG 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 8
Eco32I GATATC 1 cut(s) 2070
Eco47I GGWCC 1 cut(s) 257
Eco52I CGGCCG 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 2 cut(s) 1112, 1650
Eco91I GGTNACC 1 cut(s) 1642
EcoICRI GAGCTC 1 cut(s) 6
EcoNI CCTNNNNNAGG 1 cut(s) 1254
EcoO109I RGGNCCY 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 1642
EcoRII CCWGG 1 cut(s) 21
EcoRV GATATC 1 cut(s) 2070
EcoT22I ATGCAT 2 cut(s) 907, 1873
EcoT38I GRGCYC 1 cut(s) 8
Esp3I CGTCTC 1 cut(s) 154
FauI CCCGC 1 cut(s) 2005
FauNDI CATATG 3 cut(s) 777, 856, 1231
Fnu4HI GCNGC 5 cut(s) 156, 159, 171, 713, 1109
FokI GGATG 4 cut(s) 432, 581, 1526, 1828
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 5 cut(s) 156, 159, 171, 713, 1109
FspBI CTAG 2 cut(s) 737, 1715
GlaI GCGC 2 cut(s) 210, 956
GluI GCNGC 5 cut(s) 156, 159, 171, 713, 1109
HaeII RGCGCY 2 cut(s) 212, 958
HaeIII GGCC 9 cut(s) 228, 279, 805, 1080, 1094, 1382, 1400, 1518, 2155
HapII CCGG 3 cut(s) 180, 1519, 1647
HgaI GACGC 1 cut(s) 1718
HhaI GCGC 2 cut(s) 211, 957
Hin6I GCGC 2 cut(s) 209, 955
HinP1I GCGC 2 cut(s) 209, 955
HincII GTYRAC 5 cut(s) 247, 544, 1014, 1708, 2164
HindII GTYRAC 5 cut(s) 247, 544, 1014, 1708, 2164
HindIII AAGCTT 2 cut(s) 743, 1124
HinfI GANTC 6 cut(s) 42, 536, 577, 915, 1043, 1927
HpaI GTTAAC 2 cut(s) 544, 2164
HpaII CCGG 3 cut(s) 180, 1519, 1647
HphI GGTGA 7 cut(s) 133, 645, 1010, 1456, 1667, 1787, 1995
Hpy166II GTNNAC 6 cut(s) 247, 443, 544, 1014, 1708, 2164
Hpy188I TCNGA 5 cut(s) 41, 199, 496, 1846, 2045
Hpy8I GTNNAC 6 cut(s) 247, 443, 544, 1014, 1708, 2164
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 6 cut(s) 46, 93, 664, 1435, 1441, 1531
HpyCH4III ACNGT 5 cut(s) 293, 757, 1051, 1201, 1267
HpyCH4IV ACGT 2 cut(s) 1030, 1752
HpyF10VI GCNNNNNNNGC 7 cut(s) 167, 217, 276, 686, 1406, 1612, 2036
HpyF3I CTNAG 7 cut(s) 316, 459, 495, 808, 1055, 1437, 1950
HpySE526I ACGT 2 cut(s) 1030, 1752
HspAI GCGC 2 cut(s) 209, 955
KspAI GTTAAC 2 cut(s) 544, 2164
Kzo9I GATC 6 cut(s) 333, 867, 930, 1364, 1474, 1596
LmnI GCTCC 5 cut(s) 11, 604, 1406, 1552, 1886
Lsp1109I GCAGC 2 cut(s) 724, 1095
LweI GCATC 3 cut(s) 349, 1775, 1866
MaeI CTAG 2 cut(s) 737, 1715
MaeII ACGT 2 cut(s) 1030, 1752
MaeIII GTNAC 8 cut(s) 230, 590, 826, 859, 1031, 1267, 1642, 1738
MalI GATC 6 cut(s) 335, 869, 932, 1366, 1476, 1598
MbiI CCGCTC 1 cut(s) 220
MboI GATC 6 cut(s) 333, 867, 930, 1364, 1474, 1596
MboII GAAGA 7 cut(s) 89, 321, 1079, 1166, 1221, 1328, 1458
MflI RGATCY 1 cut(s) 1364
MhlI GDGCHC 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 1382
MluI ACGCGT 1 cut(s) 519
MluNI TGGCCA 1 cut(s) 1382
MmeI TCCRAC 5 cut(s) 222, 546, 1342, 1384, 2089
Mox20I TGGCCA 1 cut(s) 1382
Mph1103I ATGCAT 2 cut(s) 907, 1873
MscI TGGCCA 1 cut(s) 1382
MslI CAYNNNNRTG 4 cut(s) 612, 636, 1029, 1356
Msp20I TGGCCA 1 cut(s) 1382
MspI CCGG 3 cut(s) 180, 1519, 1647
MspR9I CCNGG 2 cut(s) 23, 180
Mva1269I GAATGC 1 cut(s) 193
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 1 cut(s) 521
MwoI GCNNNNNNNGC 7 cut(s) 167, 217, 276, 686, 1406, 1612, 2036
NciI CCSGG 1 cut(s) 180
NdeI CATATG 3 cut(s) 777, 856, 1231
NdeII GATC 6 cut(s) 333, 867, 930, 1364, 1474, 1596
NlaIV GGNNCC 4 cut(s) 258, 1408, 1548, 2156
NmuCI GTSAC 5 cut(s) 590, 826, 1031, 1267, 1738
NsiI ATGCAT 2 cut(s) 907, 1873
NspI RCATGY 3 cut(s) 611, 1839, 1871
OliI CACNNNNGTG 1 cut(s) 1029
PaeI GCATGC 2 cut(s) 611, 1839
PagI TCATGA 1 cut(s) 2125
PctI GAATGC 1 cut(s) 193
PfeI GAWTC 6 cut(s) 42, 536, 577, 915, 1043, 1927
PfoI TCCNGGA 1 cut(s) 21
PkrI GCNGC 5 cut(s) 157, 160, 172, 714, 1110
PpuMI RGGWCCY 1 cut(s) 257
PshBI ATTAAT 1 cut(s) 354
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 21
PspEI GGTNACC 1 cut(s) 1642
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 4 cut(s) 258, 1408, 1548, 2156
PspPI GGNCC 3 cut(s) 257, 804, 2154
PspPPI RGGWCCY 1 cut(s) 257
PstI CTGCAG 2 cut(s) 1113, 1276
PstNI CAGNNNCTG 1 cut(s) 1271
PsuI RGATCY 1 cut(s) 1364
RsaI GTAC 3 cut(s) 754, 1248, 1958
RsaNI GTAC 3 cut(s) 753, 1247, 1957
RseI CAYNNNNRTG 4 cut(s) 612, 636, 1029, 1356
SacI GAGCTC 1 cut(s) 8
SatI GCNGC 5 cut(s) 156, 159, 171, 713, 1109
Sau3AI GATC 6 cut(s) 333, 867, 930, 1364, 1474, 1596
Sau96I GGNCC 3 cut(s) 257, 804, 2154
ScaI AGTACT 1 cut(s) 1958
ScrFI CCNGG 2 cut(s) 23, 180
SduI GDGCHC 1 cut(s) 8
SfaNI GCATC 3 cut(s) 349, 1775, 1866
SfcI CTRYAG 4 cut(s) 756, 1096, 1109, 1272
SinI GGWCC 1 cut(s) 257
SmiI ATTTAAAT 1 cut(s) 1161
SmiMI CAYNNNNRTG 4 cut(s) 612, 636, 1029, 1356
SmlI CTYRAG 6 cut(s) 467, 723, 816, 1478, 1817, 1880
SmoI CTYRAG 6 cut(s) 467, 723, 816, 1478, 1817, 1880
SphI GCATGC 2 cut(s) 611, 1839
SsiI CCGC 7 cut(s) 153, 156, 159, 171, 218, 1760, 1998
SspI AATATT 2 cut(s) 511, 796
SspMI CTAG 2 cut(s) 737, 1715
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 21, 178
SwaI ATTTAAAT 1 cut(s) 1161
TaaI ACNGT 5 cut(s) 293, 757, 1051, 1201, 1267
TaiI ACGT 2 cut(s) 1033, 1755
TaqI TCGA 6 cut(s) 78, 222, 580, 870, 933, 1689
TatI WGTACW 1 cut(s) 1956
TauI GCSGC 3 cut(s) 158, 161, 173
TfiI GAWTC 6 cut(s) 42, 536, 577, 915, 1043, 1927
TscAI CASTG 2 cut(s) 417, 1276
TseFI GTSAC 5 cut(s) 590, 826, 1031, 1267, 1738
TseI GCWGC 2 cut(s) 712, 1108
Tsp45I GTSAC 5 cut(s) 590, 826, 1031, 1267, 1738
TspDTI ATGAA 5 cut(s) 890, 1152, 1659, 1817, 2142
TspGWI ACGGA 2 cut(s) 136, 1571
TspRI CASTG 2 cut(s) 417, 1276
VpaK11BI GGWCC 1 cut(s) 257
VspI ATTAAT 1 cut(s) 354
XagI CCTNNNNNAGG 1 cut(s) 1254
XapI RAATTY 1 cut(s) 1623
XceI RCATGY 3 cut(s) 611, 1839, 1871
XspI CTAG 2 cut(s) 737, 1715
ZrmI AGTACT 1 cut(s) 1958
Zsp2I ATGCAT 2 cut(s) 907, 1873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.