RLG00000034209

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000912
Physical Location & Seq
Forward (+)
38455800 .. 38458703
2904 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034209

Sequence Viewer

Length: 600 bp
ATGGGCATAACTGCTGGAATGAATAGCTGCAATTTGTTCGATTTAAGGTGGAATTCATCTGCATTTGAAGCGGGAATGGTGCTTGAATTTTTCCATTTTGCTTGCACATATGAGGATATCAACTATCTTGCCCGTGCATTAATGCTAGAAGGAGCCATGAACCAGGTACTCTGTAACATGGCTAAGGACAATGCTCATATTCCTACGGGGTCTCGTACTCATGGCCAGATAACTAAGGATGGTGAGGTTGGTTCTTCAACAATGCCTCAGAAAGTAAACCCTATTGGTTTTGAAAACAGCGTAGGCAATCATGGTGTGGCTAATGCCAATCTTTATCACCCGAGCATGAAGTTGCCTATTTCACGTTTGCAGGCTATGAAAAAATTTAACCTTCCTGTGGACGATTTGAAGCAGCTGATGAACAGAGGAATACCAGTTGGCTCGGAAACTATAACAGATTTTATGTGTCTGCTAGACTTACACTACGAACCAAAGATTGAACTTCTAAGGTATAGACCGTCCAACACTATTAGAGCAGCTGAAGAATTGGCAAGGAGATTAGACTCGGCCGTGAATGCAAATTTAAGGGAAAAGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

200

Amino Acids

22.23

Weight (kDa)

6.65

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 79 - 101 8.9e-06 Lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 71
AcoI YGGCCR 2 cut(s) 223, 567
AcsI RAATTY 4 cut(s) 52, 86, 383, 580
AcuI CTGAAG 1 cut(s) 561
AfaI GTAC 2 cut(s) 168, 217
AfiI CCNNNNNNNGG 1 cut(s) 397
AgsI TTSAA 6 cut(s) 68, 86, 258, 293, 409, 500
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 3 cut(s) 27, 415, 539
AluI AGCT 3 cut(s) 27, 415, 539
Alw26I GTCTC 1 cut(s) 216
Ama87I CYCGRG 1 cut(s) 340
AoxI GGCC 2 cut(s) 223, 567
ApeKI GCWGC 3 cut(s) 27, 412, 536
ApoI RAATTY 4 cut(s) 52, 86, 383, 580
AseI ATTAAT 1 cut(s) 140
AsuHPI GGTGA 2 cut(s) 254, 329
AvaI CYCGRG 1 cut(s) 340
BalI TGGCCA 1 cut(s) 225
BbvI GCAGC 3 cut(s) 14, 424, 548
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 554
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 216
BfaI CTAG 2 cut(s) 146, 473
BisI GCNGC 3 cut(s) 28, 413, 537
BlsI GCNGC 3 cut(s) 29, 414, 538
Bme1390I CCNGG 1 cut(s) 164
BmeT110I CYCGRG 1 cut(s) 340
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 1 cut(s) 164
Bpu10I CCTNAGC 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 216
Bsc4I CCNNNNNNNGG 1 cut(s) 397
Bse1I ACTGG 1 cut(s) 434
BseBI CCWGG 1 cut(s) 164
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 397
BseMII CTCAG 1 cut(s) 281
BseNI ACTGG 1 cut(s) 434
BseX3I CGGCCG 1 cut(s) 567
BseXI GCAGC 3 cut(s) 14, 424, 548
Bsh1285I CGRYCG 1 cut(s) 570
BshFI GGCC 2 cut(s) 225, 569
BsiEI CGRYCG 1 cut(s) 570
BsiHKCI CYCGRG 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 397
BsmAI GTCTC 1 cut(s) 216
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 2 cut(s) 225, 569
Bso31I GGTCTC 1 cut(s) 216
BsoBI CYCGRG 1 cut(s) 340
BspACI CCGC 1 cut(s) 71
BspANI GGCC 2 cut(s) 225, 569
BspCNI CTCAG 1 cut(s) 280
BspLI GGNNCC 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 216
BsrI ACTGG 1 cut(s) 434
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 1 cut(s) 519
BstC8I GCNNGC 2 cut(s) 103, 372
BstDEI CTNAG 4 cut(s) 183, 234, 267, 506
BstF5I GGATG 1 cut(s) 244
BstMAI GTCTC 1 cut(s) 216
BstMCI CGRYCG 1 cut(s) 570
BstMWI GCNNNNNNNGC 2 cut(s) 68, 575
BstNI CCWGG 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 162
BstV1I GCAGC 3 cut(s) 14, 424, 548
BstZI CGGCCG 1 cut(s) 567
BsuRI GGCC 2 cut(s) 225, 569
BtsCI GGATG 1 cut(s) 244
Cac8I GCNNGC 2 cut(s) 103, 372
CsiI ACCWGGT 1 cut(s) 162
Csp6I GTAC 2 cut(s) 167, 216
CviAII CATG 5 cut(s) 157, 178, 221, 311, 346
CviQI GTAC 2 cut(s) 167, 216
DdeI CTNAG 4 cut(s) 183, 234, 267, 506
EaeI YGGCCR 2 cut(s) 223, 567
EagI CGGCCG 1 cut(s) 567
EclXI CGGCCG 1 cut(s) 567
Eco31I GGTCTC 1 cut(s) 216
Eco32I GATATC 1 cut(s) 118
Eco52I CGGCCG 1 cut(s) 567
Eco57I CTGAAG 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 340
EcoRI GAATTC 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 162
EcoRV GATATC 1 cut(s) 118
FaeI CATG 5 cut(s) 160, 181, 224, 314, 349
FatI CATG 5 cut(s) 156, 177, 220, 310, 345
FauI CCCGC 1 cut(s) 64
FauNDI CATATG 1 cut(s) 109
Fnu4HI GCNGC 3 cut(s) 28, 413, 537
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 3 cut(s) 28, 413, 537
FspBI CTAG 2 cut(s) 146, 473
GluI GCNGC 3 cut(s) 28, 413, 537
HaeIII GGCC 2 cut(s) 225, 569
Hin1II CATG 5 cut(s) 160, 181, 224, 314, 349
HinfI GANTC 1 cut(s) 563
HphI GGTGA 2 cut(s) 254, 329
Hpy166II GTNNAC 2 cut(s) 277, 400
Hpy188I TCNGA 2 cut(s) 270, 445
Hpy8I GTNNAC 2 cut(s) 277, 400
HpyAV CCTTC 2 cut(s) 143, 401
HpyCH4III ACNGT 1 cut(s) 519
HpyCH4IV ACGT 1 cut(s) 364
HpyCH4V TGCA 6 cut(s) 30, 62, 105, 137, 370, 578
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 575
HpyF3I CTNAG 4 cut(s) 183, 234, 267, 506
HpySE526I ACGT 1 cut(s) 364
Hsp92II CATG 5 cut(s) 160, 181, 224, 314, 349
LmnI GCTCC 1 cut(s) 152
LpnPI CCDG 6 cut(s) 149, 176, 239, 356, 408, 447
Lsp1109I GCAGC 3 cut(s) 14, 424, 548
MabI ACCWGGT 1 cut(s) 162
MaeI CTAG 2 cut(s) 146, 473
MaeII ACGT 1 cut(s) 364
MaeIII GTNAC 1 cut(s) 173
MboII GAAGA 2 cut(s) 246, 554
MlsI TGGCCA 1 cut(s) 225
MluCI AATT 6 cut(s) 31, 52, 86, 383, 545, 580
MluNI TGGCCA 1 cut(s) 225
MlyI GAGTC 1 cut(s) 557
MmeI TCCRAC 1 cut(s) 546
MnlI CCTC 4 cut(s) 106, 238, 276, 419
Mox20I TGGCCA 1 cut(s) 225
MscI TGGCCA 1 cut(s) 225
MseI TTAA 4 cut(s) 44, 140, 387, 584
Msp20I TGGCCA 1 cut(s) 225
MspA1I CMGCKG 2 cut(s) 415, 539
MspR9I CCNGG 1 cut(s) 164
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 1 cut(s) 164
MwoI GCNNNNNNNGC 2 cut(s) 68, 575
NdeI CATATG 1 cut(s) 109
NlaIII CATG 5 cut(s) 160, 181, 224, 314, 349
NlaIV GGNNCC 1 cut(s) 154
NmeAIII GCCGAG 1 cut(s) 545
PctI GAATGC 1 cut(s) 580
PkrI GCNGC 3 cut(s) 29, 414, 538
PleI GAGTC 1 cut(s) 557
PpsI GAGTC 1 cut(s) 557
PshBI ATTAAT 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 1 cut(s) 154
PvuII CAGCTG 2 cut(s) 415, 539
RsaI GTAC 2 cut(s) 168, 217
RsaNI GTAC 2 cut(s) 167, 216
SaqAI TTAA 4 cut(s) 44, 140, 387, 584
SatI GCNGC 3 cut(s) 28, 413, 537
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 1 cut(s) 164
SetI ASST 9 cut(s) 29, 50, 168, 249, 367, 393, 417, 512, 541
SexAI ACCWGGT 1 cut(s) 162
Sse9I AATT 6 cut(s) 31, 52, 86, 383, 545, 580
SsiI CCGC 1 cut(s) 71
SspMI CTAG 2 cut(s) 146, 473
StyD4I CCNGG 1 cut(s) 162
TaaI ACNGT 1 cut(s) 519
TaiI ACGT 1 cut(s) 367
TaqI TCGA 1 cut(s) 39
TasI AATT 6 cut(s) 31, 52, 86, 383, 545, 580
Tru1I TTAA 4 cut(s) 44, 140, 387, 584
Tru9I TTAA 4 cut(s) 44, 140, 387, 584
TseI GCWGC 3 cut(s) 27, 412, 536
TspDTI ATGAA 6 cut(s) 35, 45, 173, 362, 392, 434
VspI ATTAAT 1 cut(s) 140
XapI RAATTY 4 cut(s) 52, 86, 383, 580
XspI CTAG 2 cut(s) 146, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.