FvH4_5g14130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000912
Physical Location & Seq
Forward (+)
7994737 .. 7996153
1417 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14130.t1

Sequence Viewer

Length: 588 bp
ATGGTTATGAACCTGGATGCAGATTCTTTAGCTCCCCTCCTAATGATGTTATCCCTCTCTTGGAAGCTGATTCTTCCGGCTCTTCCAAGCCTAGATTGGTTGTGTTTTAAGTTTCTTGTCCTTGCCTTCGGGCTCCCATGCTCATCTTGGACATGGTCTTCCCCAATAACCTCACCGATCGCCAAGTACTATATGCACACTGCACAGAAGCTGTTTCTGTTAGCGTCTCTGTCTTCTGGCGAGCTGGAGCTCTCCTCCTGGTGCTTCGAGCAACAGGAGTCTTTGTGGAACCTCAGTCAATTCATGTCCCACCTCTTCACCAACAGTCTTCTTCGTCGGAGCACGCCTAGTGGTAGTCCTAGCCACAGCTGGTCCTCCGATGACCTTCCTTCTCTTCACTTGTGTAGATCGTCTTCATGGAGAAAGCTCCTCTCTCATCAAGTGGATGAAGCTCAACGTCGAGTGGAGGAAGACCAAGAGGAGAGTATCAACATCATCCAACAAATGAGCAAAGACACCACCATCACGTGTAGGAAGTCCACCATCCAATGGAGTAAGCTCAACACCGCGCGAGTGGATGAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

22.29

Weight (kDa)

5.93

Isoelectric Point (pI)

64.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 549
AccII CGCG 2 cut(s) 569, 571
AciI CCGC 1 cut(s) 567
AcvI CACGTG 1 cut(s) 528
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 2 cut(s) 60, 549
AflIII ACRYGT 1 cut(s) 527
AjnI CCWGG 2 cut(s) 12, 257
AluBI AGCT 9 cut(s) 32, 67, 211, 244, 250, 369, 427, 452, 559
AluI AGCT 9 cut(s) 32, 67, 211, 244, 250, 369, 427, 452, 559
Alw21I GWGCWC 2 cut(s) 252, 344
Alw26I GTCTC 1 cut(s) 231
AlwNI CAGNNNCTG 1 cut(s) 211
AspLEI GCGC 1 cut(s) 571
AspS9I GGNCC 1 cut(s) 372
AsuHPI GGTGA 2 cut(s) 165, 310
AvaII GGWCC 1 cut(s) 372
BanII GRGCYC 2 cut(s) 135, 252
BarI GAAGNNNNNNTAC 2 cut(s) 397, 429
BbrPI CACGTG 1 cut(s) 528
BbsI GAAGAC 5 cut(s) 150, 225, 320, 405, 477
Bbv12I GWGCWC 2 cut(s) 252, 344
BccI CCATC 2 cut(s) 530, 551
BciT130I CCWGG 2 cut(s) 14, 259
BcoDI GTCTC 1 cut(s) 231
BfaI CTAG 3 cut(s) 92, 348, 360
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 2 cut(s) 14, 259
Bme18I GGWCC 1 cut(s) 372
BmgT120I GGNCC 1 cut(s) 372
BmiI GGNNCC 2 cut(s) 134, 290
BmrFI CCNGG 2 cut(s) 14, 259
BmsI GCATC 1 cut(s) 7
BpiI GAAGAC 5 cut(s) 150, 225, 320, 405, 477
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpmI CTGGAG 1 cut(s) 266
BsaAI YACGTR 1 cut(s) 528
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 549
BseBI CCWGG 2 cut(s) 14, 259
BseGI GGATG 5 cut(s) 22, 451, 495, 543, 583
BseLI CCNNNNNNNGG 2 cut(s) 60, 549
BseMII CTCAG 1 cut(s) 307
BseRI GAGGAG 3 cut(s) 244, 419, 494
BsgI GTGCAG 1 cut(s) 186
Bsh1236I CGCG 2 cut(s) 569, 571
Bsh1285I CGRYCG 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 252, 344
BsiSI CCGG 1 cut(s) 77
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 2 cut(s) 60, 549
BsmAI GTCTC 1 cut(s) 231
BsmBI CGTCTC 1 cut(s) 231
BsmFI GGGAC 1 cut(s) 292
Bsp1286I GDGCHC 3 cut(s) 135, 252, 344
Bsp143I GATC 2 cut(s) 177, 407
BspACI CCGC 1 cut(s) 567
BspCNI CTCAG 1 cut(s) 306
BspFNI CGCG 2 cut(s) 569, 571
BspLI GGNNCC 2 cut(s) 134, 290
BspQI GCTCTTC 1 cut(s) 87
BssMI GATC 2 cut(s) 177, 407
Bst2UI CCWGG 2 cut(s) 14, 259
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 3 cut(s) 87, 320, 399
BstBAI YACGTR 1 cut(s) 528
BstC8I GCNNGC 2 cut(s) 242, 344
BstDEI CTNAG 1 cut(s) 293
BstF5I GGATG 5 cut(s) 22, 451, 495, 543, 583
BstFNI CGCG 2 cut(s) 569, 571
BstHHI GCGC 1 cut(s) 571
BstKTI GATC 2 cut(s) 180, 410
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 2 cut(s) 177, 407
BstMCI CGRYCG 1 cut(s) 180
BstNI CCWGG 2 cut(s) 14, 259
BstSCI CCNGG 2 cut(s) 12, 257
BstUI CGCG 2 cut(s) 569, 571
BstV2I GAAGAC 5 cut(s) 150, 225, 320, 405, 477
BtsCI GGATG 5 cut(s) 22, 451, 495, 543, 583
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 2 cut(s) 242, 344
CaiI CAGNNNCTG 1 cut(s) 211
CfoI GCGC 1 cut(s) 571
Cfr13I GGNCC 1 cut(s) 372
CseI GACGC 1 cut(s) 213
Csp6I GTAC 1 cut(s) 187
CviAII CATG 4 cut(s) 138, 153, 304, 417
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 1 cut(s) 293
DpnI GATC 2 cut(s) 179, 409
DpnII GATC 2 cut(s) 177, 407
Eam1104I CTCTTC 3 cut(s) 87, 320, 399
EarI CTCTTC 3 cut(s) 87, 320, 399
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 2 cut(s) 135, 252
Eco47I GGWCC 1 cut(s) 372
Eco53kI GAGCTC 1 cut(s) 250
Eco72I CACGTG 1 cut(s) 528
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 2 cut(s) 12, 257
EcoT38I GRGCYC 2 cut(s) 135, 252
Esp3I CGTCTC 1 cut(s) 231
FaeI CATG 4 cut(s) 141, 156, 307, 420
FaiI YATR 7 cut(s) 8, 139, 154, 192, 194, 305, 418
FaqI GGGAC 1 cut(s) 292
FatI CATG 4 cut(s) 137, 152, 303, 416
FokI GGATG 4 cut(s) 29, 458, 482, 530
FriOI GRGCYC 2 cut(s) 135, 252
FspBI CTAG 3 cut(s) 92, 348, 360
GlaI GCGC 1 cut(s) 570
GsuI CTGGAG 1 cut(s) 266
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 213
HhaI GCGC 1 cut(s) 571
Hin1II CATG 4 cut(s) 141, 156, 307, 420
Hin6I GCGC 1 cut(s) 569
HinP1I GCGC 1 cut(s) 569
HinfI GANTC 3 cut(s) 23, 70, 278
HpaII CCGG 1 cut(s) 77
HphI GGTGA 2 cut(s) 165, 310
Hpy166II GTNNAC 1 cut(s) 540
Hpy188I TCNGA 2 cut(s) 339, 379
Hpy8I GTNNAC 1 cut(s) 540
Hpy99I CGWCG 2 cut(s) 339, 462
HpyAV CCTTC 3 cut(s) 136, 395, 399
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 2 cut(s) 457, 527
HpyCH4V TGCA 3 cut(s) 20, 196, 203
HpyF3I CTNAG 1 cut(s) 293
HpySE526I ACGT 2 cut(s) 457, 527
Hsp92II CATG 4 cut(s) 141, 156, 307, 420
HspAI GCGC 1 cut(s) 569
Kzo9I GATC 2 cut(s) 177, 407
LguI GCTCTTC 1 cut(s) 87
LmnI GCTCC 5 cut(s) 37, 138, 247, 339, 432
LpnPI CCDG 8 cut(s) 26, 90, 222, 230, 244, 260, 271, 355
LweI GCATC 1 cut(s) 7
MaeI CTAG 3 cut(s) 92, 348, 360
MaeII ACGT 2 cut(s) 457, 527
MalI GATC 2 cut(s) 179, 409
MboI GATC 2 cut(s) 177, 407
MhlI GDGCHC 3 cut(s) 135, 252, 344
MluCI AATT 1 cut(s) 299
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 2 cut(s) 317, 523
MseI TTAA 1 cut(s) 108
MspA1I CMGCKG 1 cut(s) 369
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 2 cut(s) 14, 259
MvaI CCWGG 2 cut(s) 14, 259
MvnI CGCG 2 cut(s) 569, 571
NdeII GATC 2 cut(s) 177, 407
NlaIII CATG 4 cut(s) 141, 156, 307, 420
NlaIV GGNNCC 2 cut(s) 134, 290
PciSI GCTCTTC 1 cut(s) 87
PfeI GAWTC 2 cut(s) 23, 70
PflFI GACNNNGTC 1 cut(s) 154
PflMI CCANNNNNTGG 1 cut(s) 549
Ple19I CGATCG 1 cut(s) 180
PleI GAGTC 1 cut(s) 286
PmaCI CACGTG 1 cut(s) 528
PmlI CACGTG 1 cut(s) 528
PpsI GAGTC 1 cut(s) 286
Ppu21I YACGTR 1 cut(s) 528
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 12, 257
PspCI CACGTG 1 cut(s) 528
PspGI CCWGG 2 cut(s) 12, 257
PspN4I GGNNCC 2 cut(s) 134, 290
PspPI GGNCC 1 cut(s) 372
PstNI CAGNNNCTG 1 cut(s) 211
PsyI GACNNNGTC 1 cut(s) 154
PvuI CGATCG 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 369
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SacI GAGCTC 1 cut(s) 252
SapI GCTCTTC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 108
Sau3AI GATC 2 cut(s) 177, 407
Sau96I GGNCC 1 cut(s) 372
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 14, 259
SduI GDGCHC 3 cut(s) 135, 252, 344
SfaNI GCATC 1 cut(s) 7
SinI GGWCC 1 cut(s) 372
Sse9I AATT 1 cut(s) 299
SsiI CCGC 1 cut(s) 567
SspMI CTAG 3 cut(s) 92, 348, 360
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 12, 257
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 2 cut(s) 460, 530
TaqI TCGA 2 cut(s) 267, 460
TasI AATT 1 cut(s) 299
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 2 cut(s) 23, 70
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 1 cut(s) 205
TspDTI ATGAA 4 cut(s) 23, 292, 405, 462
TspRI CASTG 1 cut(s) 205
Tth111I GACNNNGTC 1 cut(s) 154
Van91I CCANNNNNTGG 1 cut(s) 549
VpaK11BI GGWCC 1 cut(s) 372
XcmI CCANNNNNNNNNTGG 2 cut(s) 93, 144
XspI CTAG 3 cut(s) 92, 348, 360
ZrmI AGTACT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.