Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 113 |
| AfaI | GTAC | 2 cut(s) | 27, 232 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 208 |
| AgsI | TTSAA | 2 cut(s) | 113, 224 |
| Alw26I | GTCTC | 1 cut(s) | 77 |
| ApoI | RAATTY | 1 cut(s) | 113 |
| AseI | ATTAAT | 1 cut(s) | 167 |
| BcoDI | GTCTC | 1 cut(s) | 77 |
| BfaI | CTAG | 1 cut(s) | 173 |
| BmiI | GGNNCC | 1 cut(s) | 181 |
| BmsI | GCATC | 1 cut(s) | 43 |
| BsaAI | YACGTR | 1 cut(s) | 230 |
| BsaI | GGTCTC | 1 cut(s) | 77 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 208 |
| BseGI | GGATG | 1 cut(s) | 217 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 208 |
| BseMII | CTCAG | 1 cut(s) | 129 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 208 |
| BsmAI | GTCTC | 1 cut(s) | 77 |
| Bso31I | GGTCTC | 1 cut(s) | 77 |
| BspCNI | CTCAG | 1 cut(s) | 130 |
| BspLI | GGNNCC | 1 cut(s) | 181 |
| BspTNI | GGTCTC | 1 cut(s) | 77 |
| BstBAI | YACGTR | 1 cut(s) | 230 |
| BstC8I | GCNNGC | 1 cut(s) | 130 |
| BstDEI | CTNAG | 1 cut(s) | 138 |
| BstF5I | GGATG | 1 cut(s) | 217 |
| BstMAI | GTCTC | 1 cut(s) | 77 |
| BtsCI | GGATG | 1 cut(s) | 217 |
| Cac8I | GCNNGC | 1 cut(s) | 130 |
| Csp6I | GTAC | 2 cut(s) | 26, 231 |
| CviAII | CATG | 3 cut(s) | 92, 184, 208 |
| CviJI | RGCY | 1 cut(s) | 182 |
| CviKI_1 | RGCY | 1 cut(s) | 182 |
| CviQI | GTAC | 2 cut(s) | 26, 231 |
| DdeI | CTNAG | 1 cut(s) | 138 |
| Eco31I | GGTCTC | 1 cut(s) | 77 |
| Eco32I | GATATC | 1 cut(s) | 145 |
| EcoRV | GATATC | 1 cut(s) | 145 |
| FaeI | CATG | 3 cut(s) | 95, 187, 211 |
| FaiI | YATR | 6 cut(s) | 12, 47, 93, 185, 209, 236 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 93, 125 |
| FatI | CATG | 3 cut(s) | 91, 183, 207 |
| FokI | GGATG | 1 cut(s) | 224 |
| FspBI | CTAG | 1 cut(s) | 173 |
| Hin1II | CATG | 3 cut(s) | 95, 187, 211 |
| HinfI | GANTC | 1 cut(s) | 220 |
| Hpy188I | TCNGA | 1 cut(s) | 139 |
| HpyAV | CCTTC | 1 cut(s) | 170 |
| HpyCH4IV | ACGT | 2 cut(s) | 24, 229 |
| HpyCH4V | TGCA | 3 cut(s) | 56, 132, 164 |
| HpyF3I | CTNAG | 1 cut(s) | 138 |
| HpySE526I | ACGT | 2 cut(s) | 24, 229 |
| Hsp92II | CATG | 3 cut(s) | 95, 187, 211 |
| LmnI | GCTCC | 1 cut(s) | 179 |
| LweI | GCATC | 1 cut(s) | 43 |
| MaeI | CTAG | 1 cut(s) | 173 |
| MaeII | ACGT | 2 cut(s) | 24, 229 |
| MboII | GAAGA | 1 cut(s) | 82 |
| MluCI | AATT | 1 cut(s) | 113 |
| MnlI | CCTC | 1 cut(s) | 133 |
| MseI | TTAA | 2 cut(s) | 99, 167 |
| NlaIII | CATG | 3 cut(s) | 95, 187, 211 |
| NlaIV | GGNNCC | 1 cut(s) | 181 |
| PfeI | GAWTC | 1 cut(s) | 220 |
| Ppu21I | YACGTR | 1 cut(s) | 230 |
| PshBI | ATTAAT | 1 cut(s) | 167 |
| PspN4I | GGNNCC | 1 cut(s) | 181 |
| RsaI | GTAC | 2 cut(s) | 27, 232 |
| RsaNI | GTAC | 2 cut(s) | 26, 231 |
| SaqAI | TTAA | 2 cut(s) | 99, 167 |
| SetI | ASST | 2 cut(s) | 27, 232 |
| SfaNI | GCATC | 1 cut(s) | 43 |
| Sse9I | AATT | 1 cut(s) | 113 |
| SspMI | CTAG | 1 cut(s) | 173 |
| TaiI | ACGT | 2 cut(s) | 27, 232 |
| TasI | AATT | 1 cut(s) | 113 |
| TfiI | GAWTC | 1 cut(s) | 220 |
| Tru1I | TTAA | 2 cut(s) | 99, 167 |
| Tru9I | TTAA | 2 cut(s) | 99, 167 |
| TspDTI | ATGAA | 2 cut(s) | 196, 200 |
| VspI | ATTAAT | 1 cut(s) | 167 |
| XapI | RAATTY | 1 cut(s) | 113 |
| XspI | CTAG | 1 cut(s) | 173 |