AT1G36280

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000912
Physical Location & Seq
Forward (+)
13640508 .. 13643126
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G36280.1

Sequence Viewer

Length: 1584 bp
ATGGAGCTCAATTTATCTTCGATGGCCACCAAAATCCCTTGTTCCGGCCTTATACCCATAAGAAGTCTTTTAAACCCATCAAAATCTCTGTCTCATCTTCCAAGAGTTTCATTCTCTGTTTCTTCTCCTCATTCTCTCAAGCTAATGACTTCAACAAAGTTGATAGCAATGGCTAGTGCAAGTTCCAGAGACTTTGAGATGTCGAATTTAACAGCATTATCGCCTTTGGATGGACGTTATTGGGGAAAAGTTAAGGACTTGGCTTCTTCTATGAGCGAGTTTGGATTGATCTATTTCCGAGTACTTGTCGAGATCAAATGGCTTATTAAGCTTTCAAATATACCTCAAGTCACTGAAGTTCCAAGCTTTAGCAAAGAAGCTCATAAGTACTTGCAAGGTATAATCGATGGATTTAGTATGGATGATGCATTGCAAGTTAAGAAGATTGAGAGAGTGACAAATCACGATGTGAAAGCAGTGGAGTATTTTTTGAAACAAAAGTGTGAATCACATCCAGAGATTGCTAAGGTTCTAGAGTTTTTCCATTTCGCTTGTACGTCTGAGGACATCAACAATCTTTCCCATGCTTTGATGCTTCAAGAAGCACTTAGTTCGGTTATACTTCCTACCATGGATGAGTTGATCAAGTCTATCTCTCTGATGGCTAAGAGCTTTGCATATGTACCTATGCTTTCACGAACTCATGGGCAGCCAGCTTCACCTACAACATTGGGGAAGGAAATGGCGATTTTTGCTGTGAGGTTAAGTGTAGAAAGGAGATATCTTTCAGAAACTAAGATTAAGGGGAAATTTGCGGGTGCTGTTGGAAACTACAACGCGCATATTTCCGCGTATTCTAATATTGACTGGCCTCATGTTGCAGAGGAGTTTGTTACTTCTCTTGGATTAACATTTAACCCATACGTGACTCAGATTGAACCACATGACTATATGGCTAGACTTTTTAATACAATTAGCCAATTCAACAATGTCTTGATTGATTTTGACAGAGATATATGGAGCTACATATCTCTAGGTTACTTTAAGCAGATAACTAAAGCGGGTGAAATCGGATCATCGACAATGCCTCACAAAGTGAATCCTATTGACTTTGAGAACAGCGAAGGGAATCTAGGTAAAGCAAATGCAGAGCTTGCTTTTCTCAGCATGAAGCTTCCCATTTCACGAATGCAGCGTGATTTAACAGATTCAACTGTCTTGAGAAACATGGGTGGAGCTTTAGGACACTCTCTTCTGGCTTACAAGAGCGCGATACAGGGAATTGGGAAGCTTCAGGTTAATGAAGCTCGGTTAAAAGACGATTTGGATCATACTTGGGAAGTCCTCGCTGAACCGATACAAACTGTGATGAGGAGATATGGTGTTCCAGAGCCGTATGAGAAGCTGAAGGAGCTAACAAGAGGAAGAGCTGTGAATGAAGAAAGCATTAGAAAGTTTATTAAAAGTTTGGAATTGCCTGAAGAAGCAAAAGACCAACTTTTGAAGCTAACTCCACACACATATGTTGGCGCTGCTGCTGCATTGGCACTAGCCGTGGATGATGCTGTGCACTTGGGACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

527

Amino Acids

58.82

Weight (kDa)

7.01

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 99 - 379 1.6e-56 Lyase
ASL_C PF08328 395 - 509 1.7e-51 Adenylosuccinate lyase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 839, 851, 1271
AciI CCGC 3 cut(s) 815, 849, 1061
AclWI GGATC 2 cut(s) 1081, 1335
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 2 cut(s) 205, 809
AcuI CTGAAG 4 cut(s) 375, 1277, 1427, 1500
AdeI CACNNNGTG 2 cut(s) 469, 1096
AfaI GTAC 4 cut(s) 303, 389, 556, 684
AfiI CCNNNNNNNGG 2 cut(s) 44, 230
AgsI TTSAA 8 cut(s) 153, 336, 493, 599, 938, 985, 1212, 1504
Alw21I GWGCWC 2 cut(s) 9, 1572
Alw26I GTCTC 2 cut(s) 96, 183
Alw44I GTGCAC 1 cut(s) 1568
AlwI GGATC 2 cut(s) 1081, 1335
AoxI GGCC 3 cut(s) 24, 46, 869
ApaLI GTGCAC 1 cut(s) 1568
ApeKI GCWGC 5 cut(s) 709, 1192, 1532, 1535, 1538
ApoI RAATTY 2 cut(s) 205, 809
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AspLEI GCGC 3 cut(s) 841, 1271, 1532
AsuHPI GGTGA 2 cut(s) 711, 1076
BaeGI GKGCMC 1 cut(s) 1572
BalI TGGCCA 1 cut(s) 26
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 2 cut(s) 9, 1572
BbvI GCAGC 5 cut(s) 721, 1204, 1519, 1522, 1525
BccI CCATC 5 cut(s) 16, 85, 224, 401, 655
BceAI ACGGC 2 cut(s) 1378, 1538
BcgI CGANNNNNNTGC 2 cut(s) 594, 628
BclI TGATCA 1 cut(s) 642
BcoDI GTCTC 2 cut(s) 96, 183
BfaI CTAG 6 cut(s) 174, 533, 957, 1034, 1133, 1550
BfoI RGCGCY 1 cut(s) 1533
BisI GCNGC 5 cut(s) 710, 1193, 1533, 1536, 1539
BlsI GCNGC 5 cut(s) 711, 1194, 1534, 1537, 1540
BmcAI AGTACT 2 cut(s) 303, 389
BmsI GCATC 3 cut(s) 415, 582, 1552
Bpu10I CCTNAGC 1 cut(s) 525
BpuEI CTTGAG 3 cut(s) 122, 330, 1240
Bsa29I ATCGAT 1 cut(s) 405
BsaAI YACGTR 1 cut(s) 925
BsaBI GATNNNNATC 1 cut(s) 1326
BsaJI CCNNGG 2 cut(s) 630, 1554
BsaXI ACNNNNNCTCC 2 cut(s) 1366, 1396
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 230
Bse1I ACTGG 1 cut(s) 872
Bse3DI GCAATG 2 cut(s) 174, 428
Bse8I GATNNNNATC 1 cut(s) 1326
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 2 cut(s) 630, 1554
BseGI GGATG 5 cut(s) 235, 427, 511, 640, 1564
BseJI GATNNNNATC 1 cut(s) 1326
BseLI CCNNNNNNNGG 2 cut(s) 44, 230
BseMI GCAATG 2 cut(s) 174, 428
BseMII CTCAG 3 cut(s) 552, 944, 1177
BseNI ACTGG 1 cut(s) 872
BseRI GAGGAG 3 cut(s) 117, 899, 1387
BseSI GKGCMC 1 cut(s) 1572
BseXI GCAGC 5 cut(s) 721, 1204, 1519, 1522, 1525
Bsh1236I CGCG 3 cut(s) 839, 851, 1271
BshFI GGCC 3 cut(s) 26, 48, 871
BshVI ATCGAT 1 cut(s) 405
BsiHKAI GWGCWC 2 cut(s) 9, 1572
BsiSI CCGG 1 cut(s) 45
BslI CCNNNNNNNGG 2 cut(s) 44, 230
BsmAI GTCTC 2 cut(s) 96, 183
BsmI GAATGC 1 cut(s) 1194
BsnI GGCC 3 cut(s) 26, 48, 871
Bsp1286I GDGCHC 2 cut(s) 9, 1572
Bsp143I GATC 5 cut(s) 288, 312, 642, 1073, 1327
Bsp19I CCATGG 1 cut(s) 630
BspACI CCGC 3 cut(s) 815, 849, 1061
BspANI GGCC 3 cut(s) 26, 48, 871
BspCNI CTCAG 3 cut(s) 553, 943, 1176
BspDI ATCGAT 1 cut(s) 405
BspFNI CGCG 3 cut(s) 839, 851, 1271
BspPI GGATC 2 cut(s) 1081, 1335
BspQI GCTCTTC 1 cut(s) 1420
BsrDI GCAATG 2 cut(s) 174, 428
BsrI ACTGG 1 cut(s) 872
BssECI CCNNGG 2 cut(s) 630, 1554
BssMI GATC 5 cut(s) 288, 312, 642, 1073, 1327
BssT1I CCWWGG 1 cut(s) 630
Bst4CI ACNGT 2 cut(s) 1216, 1366
Bst6I CTCTTC 2 cut(s) 1257, 1420
BstAPI GCANNNNNTGC 1 cut(s) 1154
BstBAI YACGTR 1 cut(s) 925
BstC8I GCNNGC 2 cut(s) 714, 1155
BstDEI CTNAG 7 cut(s) 525, 561, 608, 666, 795, 930, 1163
BstDSI CCRYGG 2 cut(s) 630, 1554
BstF5I GGATG 5 cut(s) 235, 427, 511, 640, 1564
BstFNI CGCG 3 cut(s) 839, 851, 1271
BstH2I RGCGCY 1 cut(s) 1533
BstHHI GCGC 3 cut(s) 841, 1271, 1532
BstKTI GATC 5 cut(s) 291, 315, 645, 1076, 1330
BstMAI GTCTC 2 cut(s) 96, 183
BstMBI GATC 5 cut(s) 288, 312, 642, 1073, 1327
BstMWI GCNNNNNNNGC 6 cut(s) 328, 752, 1154, 1411, 1538, 1544
BstSLI GKGCMC 1 cut(s) 1572
BstUI CGCG 3 cut(s) 839, 851, 1271
BstV1I GCAGC 5 cut(s) 721, 1204, 1519, 1522, 1525
Bsu15I ATCGAT 1 cut(s) 405
BsuRI GGCC 3 cut(s) 26, 48, 871
BsuTUI ATCGAT 1 cut(s) 405
BtgI CCRYGG 2 cut(s) 630, 1554
BtsCI GGATG 5 cut(s) 235, 427, 511, 640, 1564
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 2 cut(s) 351, 483
Cac8I GCNNGC 2 cut(s) 714, 1155
CfoI GCGC 3 cut(s) 841, 1271, 1532
ClaI ATCGAT 1 cut(s) 405
Csp6I GTAC 4 cut(s) 302, 388, 555, 683
CviAII CATG 7 cut(s) 584, 631, 704, 875, 944, 1168, 1228
CviQI GTAC 4 cut(s) 302, 388, 555, 683
DdeI CTNAG 7 cut(s) 525, 561, 608, 666, 795, 930, 1163
DpnI GATC 5 cut(s) 290, 314, 644, 1075, 1329
DpnII GATC 5 cut(s) 288, 312, 642, 1073, 1327
DraI TTTAAA 1 cut(s) 72
DraIII CACNNNGTG 2 cut(s) 469, 1096
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 2 cut(s) 1257, 1420
EarI CTCTTC 2 cut(s) 1257, 1420
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 630
Eco24I GRGCYC 1 cut(s) 9
Eco32I GATATC 1 cut(s) 782
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 4 cut(s) 375, 1277, 1427, 1500
EcoICRI GAGCTC 1 cut(s) 7
EcoRV GATATC 1 cut(s) 782
EcoT14I CCWWGG 1 cut(s) 630
EcoT22I ATGCAT 1 cut(s) 430
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 630
FaeI CATG 7 cut(s) 587, 634, 707, 878, 947, 1171, 1231
FalI AAGNNNNNCTT 4 cut(s) 591, 623, 1482, 1514
FatI CATG 7 cut(s) 583, 630, 703, 874, 943, 1167, 1227
FauI CCCGC 2 cut(s) 808, 1054
FauNDI CATATG 2 cut(s) 679, 1522
FbaI TGATCA 1 cut(s) 642
Fnu4HI GCNGC 5 cut(s) 710, 1193, 1533, 1536, 1539
FokI GGATG 5 cut(s) 242, 434, 498, 647, 1571
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 5 cut(s) 710, 1193, 1533, 1536, 1539
FspBI CTAG 6 cut(s) 174, 533, 957, 1034, 1133, 1550
GlaI GCGC 3 cut(s) 840, 1270, 1531
GluI GCNGC 5 cut(s) 710, 1193, 1533, 1536, 1539
HaeII RGCGCY 1 cut(s) 1533
HaeIII GGCC 3 cut(s) 26, 48, 871
HapII CCGG 1 cut(s) 45
HhaI GCGC 3 cut(s) 841, 1271, 1532
Hin1II CATG 7 cut(s) 587, 634, 707, 878, 947, 1171, 1231
Hin6I GCGC 3 cut(s) 839, 1269, 1530
HinP1I GCGC 3 cut(s) 839, 1269, 1530
HindIII AAGCTT 4 cut(s) 329, 364, 1172, 1289
HinfI GANTC 5 cut(s) 506, 928, 1099, 1129, 1208
HpaII CCGG 1 cut(s) 45
HphI GGTGA 2 cut(s) 711, 1076
Hpy166II GTNNAC 1 cut(s) 1570
Hpy188I TCNGA 6 cut(s) 299, 562, 660, 790, 933, 1073
Hpy8I GTNNAC 1 cut(s) 1570
HpyAV CCTTC 3 cut(s) 730, 1118, 1402
HpyCH4III ACNGT 2 cut(s) 1216, 1366
HpyCH4IV ACGT 3 cut(s) 235, 557, 924
HpyF10VI GCNNNNNNNGC 6 cut(s) 328, 752, 1154, 1411, 1538, 1544
HpyF3I CTNAG 7 cut(s) 525, 561, 608, 666, 795, 930, 1163
HpySE526I ACGT 3 cut(s) 235, 557, 924
Hsp92II CATG 7 cut(s) 587, 634, 707, 878, 947, 1171, 1231
HspAI GCGC 3 cut(s) 839, 1269, 1530
Ksp22I TGATCA 1 cut(s) 642
Kzo9I GATC 5 cut(s) 288, 312, 642, 1073, 1327
LguI GCTCTTC 1 cut(s) 1420
LmnI GCTCC 4 cut(s) 4, 1020, 1235, 1411
Lsp1109I GCAGC 5 cut(s) 721, 1204, 1519, 1522, 1525
LweI GCATC 3 cut(s) 415, 582, 1552
MaeI CTAG 6 cut(s) 174, 533, 957, 1034, 1133, 1550
MaeII ACGT 3 cut(s) 235, 557, 924
MaeIII GTNAC 5 cut(s) 349, 454, 892, 925, 1037
MalI GATC 5 cut(s) 290, 314, 644, 1075, 1329
MboI GATC 5 cut(s) 288, 312, 642, 1073, 1327
MboII GAAGA 9 cut(s) 9, 89, 114, 258, 454, 1244, 1437, 1451, 1493
MhlI GDGCHC 2 cut(s) 9, 1572
MlsI TGGCCA 1 cut(s) 26
MluCI AATT 7 cut(s) 10, 205, 809, 972, 980, 1281, 1472
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 1 cut(s) 922
MmeI TCCRAC 1 cut(s) 805
Mox20I TGGCCA 1 cut(s) 26
Mph1103I ATGCAT 1 cut(s) 430
MscI TGGCCA 1 cut(s) 26
MslI CAYNNNNRTG 1 cut(s) 1521
Msp20I TGGCCA 1 cut(s) 26
MspI CCGG 1 cut(s) 45
Mva1269I GAATGC 1 cut(s) 1194
MvnI CGCG 3 cut(s) 839, 851, 1271
MwoI GCNNNNNNNGC 6 cut(s) 328, 752, 1154, 1411, 1538, 1544
NcoI CCATGG 1 cut(s) 630
NdeI CATATG 2 cut(s) 679, 1522
NdeII GATC 5 cut(s) 288, 312, 642, 1073, 1327
NlaIII CATG 7 cut(s) 587, 634, 707, 878, 947, 1171, 1231
NmuCI GTSAC 3 cut(s) 349, 454, 925
NsiI ATGCAT 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 1420
PcsI WCGNNNNNNNCGW 1 cut(s) 1192
PctI GAATGC 1 cut(s) 1194
PfeI GAWTC 4 cut(s) 506, 1099, 1129, 1208
PkrI GCNGC 5 cut(s) 711, 1194, 1534, 1537, 1540
PleI GAGTC 1 cut(s) 922
PpsI GAGTC 1 cut(s) 922
Ppu21I YACGTR 1 cut(s) 925
Psp124BI GAGCTC 1 cut(s) 9
RsaI GTAC 4 cut(s) 303, 389, 556, 684
RsaNI GTAC 4 cut(s) 302, 388, 555, 683
RseI CAYNNNNRTG 1 cut(s) 1521
SacI GAGCTC 1 cut(s) 9
SapI GCTCTTC 1 cut(s) 1420
SatI GCNGC 5 cut(s) 710, 1193, 1533, 1536, 1539
Sau3AI GATC 5 cut(s) 288, 312, 642, 1073, 1327
ScaI AGTACT 2 cut(s) 303, 389
SchI GAGTC 1 cut(s) 922
SduI GDGCHC 2 cut(s) 9, 1572
SfaNI GCATC 3 cut(s) 415, 582, 1552
SmiMI CAYNNNNRTG 1 cut(s) 1521
SmlI CTYRAG 3 cut(s) 137, 345, 1219
SmoI CTYRAG 3 cut(s) 137, 345, 1219
Sse9I AATT 7 cut(s) 10, 205, 809, 972, 980, 1281, 1472
SsiI CCGC 3 cut(s) 815, 849, 1061
SspI AATATT 1 cut(s) 862
SspMI CTAG 6 cut(s) 174, 533, 957, 1034, 1133, 1550
SstI GAGCTC 1 cut(s) 9
StyI CCWWGG 1 cut(s) 630
TaaI ACNGT 2 cut(s) 1216, 1366
TaiI ACGT 3 cut(s) 238, 560, 927
TaqI TCGA 5 cut(s) 20, 203, 309, 405, 1079
TasI AATT 7 cut(s) 10, 205, 809, 972, 980, 1281, 1472
TatI WGTACW 2 cut(s) 301, 387
TfiI GAWTC 4 cut(s) 506, 1099, 1129, 1208
TscAI CASTG 2 cut(s) 358, 483
TseFI GTSAC 3 cut(s) 349, 454, 925
TseI GCWGC 5 cut(s) 709, 1192, 1532, 1535, 1538
Tsp45I GTSAC 3 cut(s) 349, 454, 925
TspDTI ATGAA 4 cut(s) 99, 1184, 1317, 1452
TspRI CASTG 2 cut(s) 358, 483
VneI GTGCAC 1 cut(s) 1568
XapI RAATTY 2 cut(s) 205, 809
XbaI TCTAGA 1 cut(s) 532
XspI CTAG 6 cut(s) 174, 533, 957, 1034, 1133, 1550
ZrmI AGTACT 2 cut(s) 303, 389
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.