Rh5BG300400

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000912
Physical Location & Seq
Forward (+)
38744261 .. 38749542
5282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG300400.1

Sequence Viewer

Length: 873 bp
ATGAAAGCTGTTTTCAGCAAGGCAGGGGAGACAATTGGTATAAACCATATGACGATGGACGTGTCTGATCAGGTGCTTGAATTTTTCCATTTTGCTTGCACATCTGAGGATATCAACAATCTTGCCCATGCATTAATGCTAGAAGGAGCCATGAACAATGTCGTAATCCATTTCATGGATGATTTGATTCAGGCACTGTGTAACATGGCTAAGGACAATGCTCATATTCCTAGGGGGTCTTGTACTCATGGCCAGATCTCTTCGGTGGCGATAATGGGGAAATTTTCTGGTGCTGTTGGAAATTACAACACTCATCTTGTTGCATATCCTAATATTAATTGGCCCCAAATAAATAAGGACGGTCAGGTTGGTTCATCAACAATGCCTCAGAAAATAAACCCTATTGATTTTGAAAATAGCAAAGGCAATCTTAGTGTGGCTAATGCCAATCTTTATCACCCGAGCATGAAGTTGCCTATTTCACGTTTGCAGCTAAAAAGAACTTCAGTCATCCTTTCAAGTTGTTTCTGTTCCCTGCAACGAGACTTGATCGACTTATCTGTTTTGAGGAACATGGATTTAGGATTAGGGCATTCTCTTCTTGCCTACAAAAGCACACTTCAGGGAATATCAAAGGCTCAAAGCTTCAGGTATGCAGCACGTACAGTGAACGAAGAACTCATCAATGCAGAGTTGGACCTGTCTCACCCATCTTTTTCTGGACCACTGAAGGCTGTTGGGTTGGAGGAGAAAATCAGTCGATTAGTAGTCAATTCGTCTACTTTGGAATCTAAAGAGGCAAGCTTACACGAAAAGATTAAACATCTACAGAGTGATAGAGATAACCTGGACTCTAAAGGAGGATTCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

290

Amino Acids

31.8

Weight (kDa)

6.63

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 7 - 104 1e-08 Lyase
ASL_C PF08328 179 - 215 6.3e-08 Adenylosuccinate lyase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 175
AccI GTMKAC 1 cut(s) 779
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 2 cut(s) 80, 281
AcuI CTGAAG 4 cut(s) 489, 605, 631, 749
AfaI GTAC 2 cut(s) 244, 664
AfiI CCNNNNNNNGG 1 cut(s) 175
AflIII ACRYGT 1 cut(s) 60
AgsI TTSAA 3 cut(s) 80, 413, 519
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 846
AluBI AGCT 4 cut(s) 8, 493, 645, 804
AluI AGCT 4 cut(s) 8, 493, 645, 804
Alw26I GTCTC 3 cut(s) 23, 537, 708
AlwNI CAGNNNCTG 1 cut(s) 196
Ama87I CYCGRG 1 cut(s) 460
AoxI GGCC 2 cut(s) 250, 341
ApeKI GCWGC 2 cut(s) 490, 656
ApoI RAATTY 2 cut(s) 80, 281
AseI ATTAAT 2 cut(s) 134, 336
AspA2I CCTAGG 1 cut(s) 230
AspS9I GGNCC 3 cut(s) 342, 697, 722
AsuHPI GGTGA 2 cut(s) 449, 698
AvaI CYCGRG 1 cut(s) 460
AvaII GGWCC 2 cut(s) 697, 722
AvrII CCTAGG 1 cut(s) 230
BalI TGGCCA 1 cut(s) 252
BbvI GCAGC 2 cut(s) 502, 668
BccI CCATC 2 cut(s) 49, 718
BciT130I CCWGG 1 cut(s) 848
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 3 cut(s) 23, 537, 708
BfaI CTAG 2 cut(s) 140, 231
BfmI CTRYAG 1 cut(s) 827
BglII AGATCT 1 cut(s) 255
BisI GCNGC 2 cut(s) 491, 657
BlnI CCTAGG 1 cut(s) 230
BlsI GCNGC 2 cut(s) 492, 658
Bme1390I CCNGG 1 cut(s) 848
Bme18I GGWCC 2 cut(s) 697, 722
BmeT110I CYCGRG 1 cut(s) 460
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 342, 697, 722
BmiI GGNNCC 2 cut(s) 148, 344
BmrFI CCNGG 1 cut(s) 848
Bpu10I CCTNAGC 1 cut(s) 210
BsaAI YACGTR 1 cut(s) 662
BsaJI CCNNGG 1 cut(s) 230
BsaXI ACNNNNNCTCC 1 cut(s) 852
Bsc4I CCNNNNNNNGG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 848
BseDI CCNNGG 1 cut(s) 230
BseGI GGATG 2 cut(s) 184, 510
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMII CTCAG 2 cut(s) 96, 401
BseRI GAGGAG 1 cut(s) 761
BseXI GCAGC 2 cut(s) 502, 668
BshFI GGCC 2 cut(s) 252, 343
BsiHKCI CYCGRG 1 cut(s) 460
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 3 cut(s) 23, 537, 708
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 2 cut(s) 252, 343
BsoBI CYCGRG 1 cut(s) 460
Bsp143I GATC 3 cut(s) 67, 255, 549
BspANI GGCC 2 cut(s) 252, 343
BspCNI CTCAG 2 cut(s) 97, 400
BspLI GGNNCC 2 cut(s) 148, 344
BssECI CCNNGG 1 cut(s) 230
BssMI GATC 3 cut(s) 67, 255, 549
BssT1I CCWWGG 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 848
Bst4CI ACNGT 3 cut(s) 198, 362, 667
Bst6I CTCTTC 2 cut(s) 265, 603
BstBAI YACGTR 1 cut(s) 662
BstC8I GCNNGC 2 cut(s) 97, 802
BstDEI CTNAG 4 cut(s) 105, 210, 387, 431
BstF5I GGATG 2 cut(s) 184, 510
BstKTI GATC 3 cut(s) 70, 258, 552
BstMAI GTCTC 3 cut(s) 23, 537, 708
BstMBI GATC 3 cut(s) 67, 255, 549
BstNI CCWGG 1 cut(s) 848
BstSCI CCNGG 1 cut(s) 846
BstSFI CTRYAG 1 cut(s) 827
BstV1I GCAGC 2 cut(s) 502, 668
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BsuRI GGCC 2 cut(s) 252, 343
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 2 cut(s) 184, 510
BtsIMutI CAGTG 3 cut(s) 194, 672, 725
Cac8I GCNNGC 2 cut(s) 97, 802
CaiI CAGNNNCTG 1 cut(s) 196
Cfr13I GGNCC 3 cut(s) 342, 697, 722
Csp6I GTAC 2 cut(s) 243, 663
CviAII CATG 7 cut(s) 128, 151, 175, 205, 248, 466, 574
CviQI GTAC 2 cut(s) 243, 663
DdeI CTNAG 4 cut(s) 105, 210, 387, 431
DpnI GATC 3 cut(s) 69, 257, 551
DpnII GATC 3 cut(s) 67, 255, 549
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 2 cut(s) 265, 603
EarI CTCTTC 2 cut(s) 265, 603
Eco130I CCWWGG 1 cut(s) 230
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 2 cut(s) 697, 722
Eco57I CTGAAG 4 cut(s) 489, 605, 631, 749
Eco88I CYCGRG 1 cut(s) 460
EcoRII CCWGG 1 cut(s) 846
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 230
EcoT22I ATGCAT 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 230
FaeI CATG 7 cut(s) 131, 154, 178, 208, 251, 469, 577
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FatI CATG 7 cut(s) 127, 150, 174, 204, 247, 465, 573
FauNDI CATATG 1 cut(s) 48
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 779
Fnu4HI GCNGC 2 cut(s) 491, 657
FokI GGATG 2 cut(s) 191, 497
Fsp4HI GCNGC 2 cut(s) 491, 657
FspBI CTAG 2 cut(s) 140, 231
GluI GCNGC 2 cut(s) 491, 657
HaeIII GGCC 2 cut(s) 252, 343
Hin1II CATG 7 cut(s) 131, 154, 178, 208, 251, 469, 577
HindIII AAGCTT 2 cut(s) 643, 802
HinfI GANTC 4 cut(s) 187, 788, 851, 864
HphI GGTGA 2 cut(s) 449, 698
Hpy166II GTNNAC 2 cut(s) 670, 780
Hpy188I TCNGA 3 cut(s) 67, 106, 390
Hpy188III TCNNGA 1 cut(s) 720
Hpy8I GTNNAC 2 cut(s) 670, 780
HpyAV CCTTC 2 cut(s) 137, 724
HpyCH4III ACNGT 3 cut(s) 198, 362, 667
HpyCH4IV ACGT 3 cut(s) 60, 484, 661
HpyCH4V TGCA 7 cut(s) 99, 131, 323, 490, 538, 656, 689
HpyF3I CTNAG 4 cut(s) 105, 210, 387, 431
HpySE526I ACGT 3 cut(s) 60, 484, 661
Hsp92II CATG 7 cut(s) 131, 154, 178, 208, 251, 469, 577
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 3 cut(s) 67, 255, 549
LmnI GCTCC 1 cut(s) 146
Lsp1109I GCAGC 2 cut(s) 502, 668
MaeI CTAG 2 cut(s) 140, 231
MaeII ACGT 3 cut(s) 60, 484, 661
MaeIII GTNAC 1 cut(s) 200
MalI GATC 3 cut(s) 69, 257, 551
MboI GATC 3 cut(s) 67, 255, 549
MboII GAAGA 3 cut(s) 252, 590, 686
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 1 cut(s) 255
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 6 cut(s) 33, 80, 281, 301, 337, 772
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 845
MmeI TCCRAC 3 cut(s) 277, 675, 723
MnlI CCTC 6 cut(s) 100, 396, 561, 739, 790, 854
Mox20I TGGCCA 1 cut(s) 252
Mph1103I ATGCAT 1 cut(s) 133
MscI TGGCCA 1 cut(s) 252
MseI TTAA 4 cut(s) 134, 336, 819, 871
Msp20I TGGCCA 1 cut(s) 252
MspR9I CCNGG 1 cut(s) 848
MunI CAATTG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 1 cut(s) 848
NdeI CATATG 1 cut(s) 48
NdeII GATC 3 cut(s) 67, 255, 549
NlaIII CATG 7 cut(s) 131, 154, 178, 208, 251, 469, 577
NlaIV GGNNCC 2 cut(s) 148, 344
NsiI ATGCAT 1 cut(s) 133
PctI GAATGC 1 cut(s) 592
PfeI GAWTC 3 cut(s) 187, 788, 864
PflMI CCANNNNNTGG 1 cut(s) 175
PkrI GCNGC 2 cut(s) 492, 658
PleI GAGTC 1 cut(s) 845
PpsI GAGTC 1 cut(s) 845
Ppu21I YACGTR 1 cut(s) 662
PshBI ATTAAT 2 cut(s) 134, 336
Psp6I CCWGG 1 cut(s) 846
PspGI CCWGG 1 cut(s) 846
PspN4I GGNNCC 2 cut(s) 148, 344
PspPI GGNCC 3 cut(s) 342, 697, 722
PstNI CAGNNNCTG 1 cut(s) 196
PsuI RGATCY 1 cut(s) 255
RsaI GTAC 2 cut(s) 244, 664
RsaNI GTAC 2 cut(s) 243, 663
SaqAI TTAA 4 cut(s) 134, 336, 819, 871
SatI GCNGC 2 cut(s) 491, 657
Sau3AI GATC 3 cut(s) 67, 255, 549
Sau96I GGNCC 3 cut(s) 342, 697, 722
SchI GAGTC 1 cut(s) 845
ScrFI CCNGG 1 cut(s) 848
SfcI CTRYAG 1 cut(s) 827
SinI GGWCC 2 cut(s) 697, 722
Sse9I AATT 6 cut(s) 33, 80, 281, 301, 337, 772
SspI AATATT 1 cut(s) 334
SspMI CTAG 2 cut(s) 140, 231
StyD4I CCNGG 1 cut(s) 846
StyI CCWWGG 1 cut(s) 230
TaaI ACNGT 3 cut(s) 198, 362, 667
TaiI ACGT 3 cut(s) 63, 487, 664
TaqI TCGA 2 cut(s) 552, 760
TasI AATT 6 cut(s) 33, 80, 281, 301, 337, 772
TatI WGTACW 1 cut(s) 242
TfiI GAWTC 3 cut(s) 187, 788, 864
Tru1I TTAA 4 cut(s) 134, 336, 819, 871
Tru9I TTAA 4 cut(s) 134, 336, 819, 871
TscAI CASTG 3 cut(s) 201, 672, 732
TseI GCWGC 2 cut(s) 490, 656
TspDTI ATGAA 5 cut(s) 17, 163, 167, 363, 482
TspRI CASTG 3 cut(s) 201, 672, 732
Van91I CCANNNNNTGG 1 cut(s) 175
VpaK11BI GGWCC 2 cut(s) 697, 722
VspI ATTAAT 2 cut(s) 134, 336
XapI RAATTY 2 cut(s) 80, 281
XmaJI CCTAGG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 779
XspI CTAG 2 cut(s) 140, 231
Zsp2I ATGCAT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.