Rh5DG309400

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000912
Physical Location & Seq
Forward (+)
39509076 .. 39511722
2647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG309400.1

Sequence Viewer

Length: 867 bp
ATGAAAGCTGTTTTCAGCAAGGCAGGGGAGACAATTGGTATAAACCATATGACGATGGACGTGTCTGATCAGGTGCTTGAATTTTTCCATTTTGCTTGCACATCTGAGGATATCAACAATCTTGCCCATGCATTAATGCTAGAAGGAGCCATGAACAATGTCGTAATCCATTTCATGGATGATTTGATTCAGGCACTGTGTAACATGGCTAAGGACAATGCTCATATTCCTAGGGGGTCTTGTACTCATGGCCAGATCTCTTCGGTGGCGATAATGGGGAAATTTTCTGGTGCTGTTGGAAATTACAACACTCATCTTGTTGCATATCCTAATATTAATTGGCCCCAAATAAATAAGGACGGTCAGGTTGGTTCATCAACAATGCCTCAGAAAATAAACCCTATTGATTTTGAAAATAGCAAAGGCAATCTTAGTGTGGCTAATGCCAATCTTTATCACCCGAGCATGAAGTTGCCTATTTCACGTTTGCAGCTAAAAAGAACTTCAGTCATCCTTTCAAGTTGTTTCTGTTCCCTGCAACGAGACTTGATCGACTTATCTGTTTTGAGGAACATGGATTTAGGATTAGGGCATTCTCTTCTTGCCTACAAAAGCACACTTCAGGGAATATCAAAGGCTCAAAGCTTCAGGTATGCAGCACGTACAGTGAACGAAGAACTCATCAATGCAGAGTTGGACCTGTCTCACCCATCTTTTTCTGGACCACTGAAGGCTGCTATGAAAAATTTTAATCTTCCTGAGGACGATTTGAAGCAGCTGATGAACAAAGGAATACCAGTTGGCTCAGAAACTATAACAGATTTTTATGTGTCGACTGCTGGACTTACACTACGAACCAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

288

Amino Acids

31.58

Weight (kDa)

6.54

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 7 - 104 1e-08 Lyase
ASL_C PF08328 179 - 275 1.8e-09 Adenylosuccinate lyase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 175
AccI GTMKAC 1 cut(s) 833
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 3 cut(s) 80, 281, 745
AcuI CTGAAG 4 cut(s) 489, 605, 631, 749
AfaI GTAC 2 cut(s) 244, 664
AfiI CCNNNNNNNGG 1 cut(s) 175
AflIII ACRYGT 1 cut(s) 60
AgsI TTSAA 4 cut(s) 80, 413, 519, 772
AjiI CACGTC 1 cut(s) 61
AluBI AGCT 4 cut(s) 8, 493, 645, 778
AluI AGCT 4 cut(s) 8, 493, 645, 778
Alw26I GTCTC 3 cut(s) 23, 537, 708
AlwNI CAGNNNCTG 1 cut(s) 196
Ama87I CYCGRG 1 cut(s) 460
AoxI GGCC 2 cut(s) 250, 341
ApeKI GCWGC 4 cut(s) 490, 656, 734, 775
ApoI RAATTY 3 cut(s) 80, 281, 745
AseI ATTAAT 2 cut(s) 134, 336
AspA2I CCTAGG 1 cut(s) 230
AspS9I GGNCC 3 cut(s) 342, 697, 722
AsuHPI GGTGA 2 cut(s) 449, 698
AvaI CYCGRG 1 cut(s) 460
AvaII GGWCC 2 cut(s) 697, 722
AvrII CCTAGG 1 cut(s) 230
AxyI CCTNAGG 1 cut(s) 759
BalI TGGCCA 1 cut(s) 252
BbvI GCAGC 4 cut(s) 502, 668, 721, 787
BccI CCATC 2 cut(s) 49, 718
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 3 cut(s) 23, 537, 708
BfaI CTAG 2 cut(s) 140, 231
BglII AGATCT 1 cut(s) 255
BisI GCNGC 4 cut(s) 491, 657, 735, 776
BlnI CCTAGG 1 cut(s) 230
BlsI GCNGC 4 cut(s) 492, 658, 736, 777
Bme18I GGWCC 2 cut(s) 697, 722
BmeT110I CYCGRG 1 cut(s) 460
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 342, 697, 722
BmiI GGNNCC 2 cut(s) 148, 344
Bpu10I CCTNAGC 1 cut(s) 210
BsaAI YACGTR 1 cut(s) 662
BsaJI CCNNGG 1 cut(s) 230
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 797
Bse21I CCTNAGG 1 cut(s) 759
BseDI CCNNGG 1 cut(s) 230
BseGI GGATG 2 cut(s) 184, 510
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMII CTCAG 4 cut(s) 96, 401, 750, 819
BseNI ACTGG 1 cut(s) 797
BseXI GCAGC 4 cut(s) 502, 668, 721, 787
BshFI GGCC 2 cut(s) 252, 343
BsiHKCI CYCGRG 1 cut(s) 460
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 3 cut(s) 23, 537, 708
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 2 cut(s) 252, 343
BsoBI CYCGRG 1 cut(s) 460
Bsp143I GATC 3 cut(s) 67, 255, 549
BspANI GGCC 2 cut(s) 252, 343
BspCNI CTCAG 4 cut(s) 97, 400, 751, 818
BspLI GGNNCC 2 cut(s) 148, 344
BsrI ACTGG 1 cut(s) 797
BssECI CCNNGG 1 cut(s) 230
BssMI GATC 3 cut(s) 67, 255, 549
BssT1I CCWWGG 1 cut(s) 230
Bst4CI ACNGT 3 cut(s) 198, 362, 667
Bst6I CTCTTC 2 cut(s) 265, 603
BstBAI YACGTR 1 cut(s) 662
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 6 cut(s) 105, 210, 387, 431, 759, 805
BstF5I GGATG 2 cut(s) 184, 510
BstKTI GATC 3 cut(s) 70, 258, 552
BstMAI GTCTC 3 cut(s) 23, 537, 708
BstMBI GATC 3 cut(s) 67, 255, 549
BstV1I GCAGC 4 cut(s) 502, 668, 721, 787
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
Bsu36I CCTNAGG 1 cut(s) 759
BsuRI GGCC 2 cut(s) 252, 343
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 2 cut(s) 184, 510
BtsIMutI CAGTG 3 cut(s) 194, 672, 725
Cac8I GCNNGC 1 cut(s) 97
CaiI CAGNNNCTG 1 cut(s) 196
Cfr13I GGNCC 3 cut(s) 342, 697, 722
Csp6I GTAC 2 cut(s) 243, 663
CviAII CATG 7 cut(s) 128, 151, 175, 205, 248, 466, 574
CviQI GTAC 2 cut(s) 243, 663
DdeI CTNAG 6 cut(s) 105, 210, 387, 431, 759, 805
DpnI GATC 3 cut(s) 69, 257, 551
DpnII GATC 3 cut(s) 67, 255, 549
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 2 cut(s) 265, 603
EarI CTCTTC 2 cut(s) 265, 603
Eco130I CCWWGG 1 cut(s) 230
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 2 cut(s) 697, 722
Eco57I CTGAAG 4 cut(s) 489, 605, 631, 749
Eco81I CCTNAGG 1 cut(s) 759
Eco88I CYCGRG 1 cut(s) 460
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 230
EcoT22I ATGCAT 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 230
FaeI CATG 7 cut(s) 131, 154, 178, 208, 251, 469, 577
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FatI CATG 7 cut(s) 127, 150, 174, 204, 247, 465, 573
FauNDI CATATG 1 cut(s) 48
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 833
Fnu4HI GCNGC 4 cut(s) 491, 657, 735, 776
FokI GGATG 2 cut(s) 191, 497
Fsp4HI GCNGC 4 cut(s) 491, 657, 735, 776
FspBI CTAG 2 cut(s) 140, 231
GluI GCNGC 4 cut(s) 491, 657, 735, 776
HaeIII GGCC 2 cut(s) 252, 343
Hin1II CATG 7 cut(s) 131, 154, 178, 208, 251, 469, 577
HincII GTYRAC 1 cut(s) 834
HindII GTYRAC 1 cut(s) 834
HindIII AAGCTT 1 cut(s) 643
HinfI GANTC 1 cut(s) 187
HphI GGTGA 2 cut(s) 449, 698
Hpy166II GTNNAC 2 cut(s) 670, 834
Hpy188I TCNGA 4 cut(s) 67, 106, 390, 808
Hpy188III TCNNGA 2 cut(s) 720, 758
Hpy8I GTNNAC 2 cut(s) 670, 834
HpyAV CCTTC 2 cut(s) 137, 724
HpyCH4III ACNGT 3 cut(s) 198, 362, 667
HpyCH4IV ACGT 3 cut(s) 60, 484, 661
HpyCH4V TGCA 7 cut(s) 99, 131, 323, 490, 538, 656, 689
HpyF3I CTNAG 6 cut(s) 105, 210, 387, 431, 759, 805
HpySE526I ACGT 3 cut(s) 60, 484, 661
Hsp92II CATG 7 cut(s) 131, 154, 178, 208, 251, 469, 577
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 3 cut(s) 67, 255, 549
LmnI GCTCC 1 cut(s) 146
Lsp1109I GCAGC 4 cut(s) 502, 668, 721, 787
MaeI CTAG 2 cut(s) 140, 231
MaeII ACGT 3 cut(s) 60, 484, 661
MaeIII GTNAC 1 cut(s) 200
MalI GATC 3 cut(s) 69, 257, 551
MboI GATC 3 cut(s) 67, 255, 549
MboII GAAGA 4 cut(s) 252, 590, 686, 746
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 1 cut(s) 255
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 6 cut(s) 33, 80, 281, 301, 337, 745
MluNI TGGCCA 1 cut(s) 252
MmeI TCCRAC 2 cut(s) 277, 675
MnlI CCTC 4 cut(s) 100, 396, 561, 754
Mox20I TGGCCA 1 cut(s) 252
Mph1103I ATGCAT 1 cut(s) 133
MscI TGGCCA 1 cut(s) 252
MseI TTAA 3 cut(s) 134, 336, 750
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 778
MunI CAATTG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 592
NdeI CATATG 1 cut(s) 48
NdeII GATC 3 cut(s) 67, 255, 549
NlaIII CATG 7 cut(s) 131, 154, 178, 208, 251, 469, 577
NlaIV GGNNCC 2 cut(s) 148, 344
NsiI ATGCAT 1 cut(s) 133
PctI GAATGC 1 cut(s) 592
PfeI GAWTC 1 cut(s) 187
PflMI CCANNNNNTGG 1 cut(s) 175
PkrI GCNGC 4 cut(s) 492, 658, 736, 777
Ppu21I YACGTR 1 cut(s) 662
PshBI ATTAAT 2 cut(s) 134, 336
PspN4I GGNNCC 2 cut(s) 148, 344
PspPI GGNCC 3 cut(s) 342, 697, 722
PstNI CAGNNNCTG 1 cut(s) 196
PsuI RGATCY 1 cut(s) 255
PvuII CAGCTG 1 cut(s) 778
RsaI GTAC 2 cut(s) 244, 664
RsaNI GTAC 2 cut(s) 243, 663
SalI GTCGAC 1 cut(s) 832
SaqAI TTAA 3 cut(s) 134, 336, 750
SatI GCNGC 4 cut(s) 491, 657, 735, 776
Sau3AI GATC 3 cut(s) 67, 255, 549
Sau96I GGNCC 3 cut(s) 342, 697, 722
SinI GGWCC 2 cut(s) 697, 722
Sse9I AATT 6 cut(s) 33, 80, 281, 301, 337, 745
SspI AATATT 1 cut(s) 334
SspMI CTAG 2 cut(s) 140, 231
StyI CCWWGG 1 cut(s) 230
TaaI ACNGT 3 cut(s) 198, 362, 667
TaiI ACGT 3 cut(s) 63, 487, 664
TaqI TCGA 2 cut(s) 552, 833
TasI AATT 6 cut(s) 33, 80, 281, 301, 337, 745
TatI WGTACW 1 cut(s) 242
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 3 cut(s) 134, 336, 750
Tru9I TTAA 3 cut(s) 134, 336, 750
TscAI CASTG 3 cut(s) 201, 672, 732
TseI GCWGC 4 cut(s) 490, 656, 734, 775
TspDTI ATGAA 7 cut(s) 17, 163, 167, 363, 482, 755, 797
TspRI CASTG 3 cut(s) 201, 672, 732
Van91I CCANNNNNTGG 1 cut(s) 175
VpaK11BI GGWCC 2 cut(s) 697, 722
VspI ATTAAT 2 cut(s) 134, 336
XapI RAATTY 3 cut(s) 80, 281, 745
XmaJI CCTAGG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 833
XspI CTAG 2 cut(s) 140, 231
Zsp2I ATGCAT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.