Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 606 |
| Acc65I | GGTACC | 1 cut(s) | 1732 |
| AccB1I | GGYRCC | 1 cut(s) | 1732 |
| AccBSI | CCGCTC | 1 cut(s) | 1858 |
| AccI | GTMKAC | 4 cut(s) | 33, 680, 1539, 2859 |
| AccIII | TCCGGA | 1 cut(s) | 2636 |
| AciI | CCGC | 7 cut(s) | 235, 896, 1403, 1850, 1858, 2459, 2835 |
| AclWI | GGATC | 3 cut(s) | 76, 352, 2315 |
| AcoI | YGGCCR | 4 cut(s) | 768, 893, 2460, 2847 |
| AcuI | CTGAAG | 3 cut(s) | 210, 1350, 1740 |
| AcvI | CACGTG | 1 cut(s) | 134 |
| AfaI | GTAC | 9 cut(s) | 378, 1213, 1249, 1323, 1338, 1615, 1734, 2712, 2778 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 690, 2487, 2614 |
| AflII | CTTAAG | 1 cut(s) | 1253 |
| AflIII | ACRYGT | 1 cut(s) | 2400 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2729 |
| AhlI | ACTAGT | 1 cut(s) | 2435 |
| AjnI | CCWGG | 3 cut(s) | 838, 1588, 2230 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1095, 1127 |
| Alw21I | GWGCWC | 2 cut(s) | 558, 2644 |
| Alw26I | GTCTC | 6 cut(s) | 366, 745, 956, 1367, 2379, 2654 |
| AlwI | GGATC | 3 cut(s) | 76, 352, 2315 |
| Ama87I | CYCGRG | 1 cut(s) | 2585 |
| Aor13HI | TCCGGA | 1 cut(s) | 2636 |
| AoxI | GGCC | 7 cut(s) | 768, 893, 957, 1592, 2243, 2460, 2847 |
| ApeKI | GCWGC | 4 cut(s) | 1118, 1556, 1847, 2164 |
| AseI | ATTAAT | 2 cut(s) | 888, 2238 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2337 |
| Asp718I | GGTACC | 1 cut(s) | 1732 |
| AspA2I | CCTAGG | 1 cut(s) | 1736 |
| AspLEI | GCGC | 1 cut(s) | 607 |
| AspS9I | GGNCC | 1 cut(s) | 1592 |
| AsuC2I | CCSGG | 1 cut(s) | 2305 |
| AsuHPI | GGTGA | 6 cut(s) | 284, 305, 735, 2204, 2542, 2707 |
| AsuII | TTCGAA | 2 cut(s) | 1198, 1351 |
| AvaI | CYCGRG | 1 cut(s) | 2585 |
| AvrII | CCTAGG | 1 cut(s) | 1736 |
| AxyI | CCTNAGG | 1 cut(s) | 173 |
| BaeGI | GKGCMC | 1 cut(s) | 172 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1716, 1724, 1749, 1757 |
| BalI | TGGCCA | 2 cut(s) | 770, 2849 |
| BanI | GGYRCC | 1 cut(s) | 1732 |
| BanII | GRGCYC | 1 cut(s) | 2644 |
| BauI | CACGAG | 1 cut(s) | 1289 |
| BbrPI | CACGTG | 1 cut(s) | 134 |
| BbsI | GAAGAC | 2 cut(s) | 138, 1182 |
| Bbv12I | GWGCWC | 2 cut(s) | 558, 2644 |
| BbvCI | CCTCAGC | 1 cut(s) | 906 |
| BbvI | GCAGC | 4 cut(s) | 1130, 1568, 1834, 2176 |
| BccI | CCATC | 2 cut(s) | 1775, 2375 |
| BceAI | ACGGC | 1 cut(s) | 2852 |
| BciT130I | CCWGG | 3 cut(s) | 840, 1590, 2232 |
| BclI | TGATCA | 2 cut(s) | 487, 666 |
| BcnI | CCSGG | 1 cut(s) | 2305 |
| BcoDI | GTCTC | 6 cut(s) | 366, 745, 956, 1367, 2379, 2654 |
| BcuI | ACTAGT | 1 cut(s) | 2435 |
| BfmI | CTRYAG | 3 cut(s) | 313, 846, 1368 |
| BfrI | CTTAAG | 1 cut(s) | 1253 |
| BglII | AGATCT | 1 cut(s) | 1160 |
| BisI | GCNGC | 7 cut(s) | 896, 1119, 1557, 1848, 2165, 2460, 2835 |
| BlnI | CCTAGG | 1 cut(s) | 1736 |
| BlsI | GCNGC | 7 cut(s) | 897, 1120, 1558, 1849, 2166, 2461, 2836 |
| Bme1390I | CCNGG | 4 cut(s) | 840, 1590, 2232, 2305 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2729 |
| BmeT110I | CYCGRG | 1 cut(s) | 2585 |
| BmgT120I | GGNCC | 1 cut(s) | 1592 |
| BmiI | GGNNCC | 4 cut(s) | 1638, 1734, 1978, 2833 |
| BmrFI | CCNGG | 4 cut(s) | 840, 1590, 2232, 2305 |
| BmsI | GCATC | 6 cut(s) | 127, 175, 238, 515, 616, 1789 |
| BpiI | GAAGAC | 2 cut(s) | 138, 1182 |
| BpmI | CTGGAG | 1 cut(s) | 2189 |
| Bpu10I | CCTNAGC | 4 cut(s) | 549, 906, 1899, 2483 |
| Bpu14I | TTCGAA | 2 cut(s) | 1198, 1351 |
| BpuEI | CTTGAG | 5 cut(s) | 97, 798, 1841, 2232, 2789 |
| BpuMI | CCSGG | 1 cut(s) | 2305 |
| Bsa29I | ATCGAT | 2 cut(s) | 427, 1794 |
| BsaAI | YACGTR | 1 cut(s) | 134 |
| BsaBI | GATNNNNATC | 2 cut(s) | 529, 1879 |
| BsaI | GGTCTC | 2 cut(s) | 745, 1367 |
| BsaJI | CCNNGG | 7 cut(s) | 255, 1588, 1589, 1736, 1980, 2004, 2739 |
| BsaWI | WCCGGW | 2 cut(s) | 2636, 2717 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 26, 56, 2181, 2211, 2631, 2661 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 690, 2487, 2614 |
| Bse1I | ACTGG | 2 cut(s) | 2353, 2463 |
| Bse21I | CCTNAGG | 1 cut(s) | 173 |
| Bse3DI | GCAATG | 2 cut(s) | 681, 2034 |
| Bse8I | GATNNNNATC | 2 cut(s) | 529, 1879 |
| BseAI | TCCGGA | 1 cut(s) | 2636 |
| BseBI | CCWGG | 3 cut(s) | 840, 1590, 2232 |
| BseCI | ATCGAT | 2 cut(s) | 427, 1794 |
| BseDI | CCNNGG | 7 cut(s) | 255, 1588, 1589, 1736, 1980, 2004, 2739 |
| BseGI | GGATG | 3 cut(s) | 1609, 1885, 2014 |
| BseJI | GATNNNNATC | 2 cut(s) | 529, 1879 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 690, 2487, 2614 |
| BseMI | GCAATG | 2 cut(s) | 681, 2034 |
| BseMII | CTCAG | 6 cut(s) | 413, 512, 897, 945, 1926, 2474 |
| BseNI | ACTGG | 2 cut(s) | 2353, 2463 |
| BseRI | GAGGAG | 2 cut(s) | 1313, 1952 |
| BseSI | GKGCMC | 1 cut(s) | 172 |
| BseXI | GCAGC | 4 cut(s) | 1130, 1568, 1834, 2176 |
| BsgI | GTGCAG | 1 cut(s) | 1137 |
| Bsh1285I | CGRYCG | 1 cut(s) | 216 |
| BshFI | GGCC | 7 cut(s) | 770, 895, 959, 1594, 2245, 2462, 2849 |
| BshNI | GGYRCC | 1 cut(s) | 1732 |
| BshVI | ATCGAT | 2 cut(s) | 427, 1794 |
| BsiEI | CGRYCG | 1 cut(s) | 216 |
| BsiHKAI | GWGCWC | 2 cut(s) | 558, 2644 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2585 |
| BsiSI | CCGG | 3 cut(s) | 2305, 2637, 2718 |
| BslFI | GGGAC | 6 cut(s) | 3, 191, 707, 740, 1058, 2669 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 690, 2487, 2614 |
| BsmAI | GTCTC | 6 cut(s) | 366, 745, 956, 1367, 2379, 2654 |
| BsmBI | CGTCTC | 3 cut(s) | 366, 2379, 2654 |
| BsmFI | GGGAC | 6 cut(s) | 3, 191, 707, 740, 1058, 2669 |
| BsmI | GAATGC | 1 cut(s) | 2539 |
| BsnI | GGCC | 7 cut(s) | 770, 895, 959, 1594, 2245, 2462, 2849 |
| Bso31I | GGTCTC | 2 cut(s) | 745, 1367 |
| BsoBI | CYCGRG | 1 cut(s) | 2585 |
| Bsp119I | TTCGAA | 2 cut(s) | 1198, 1351 |
| Bsp1286I | GDGCHC | 3 cut(s) | 172, 558, 2644 |
| Bsp13I | TCCGGA | 1 cut(s) | 2636 |
| Bsp19I | CCATGG | 2 cut(s) | 1980, 2004 |
| BspACI | CCGC | 7 cut(s) | 235, 896, 1403, 1850, 1858, 2459, 2835 |
| BspANI | GGCC | 7 cut(s) | 770, 895, 959, 1594, 2245, 2462, 2849 |
| BspCNI | CTCAG | 6 cut(s) | 412, 513, 898, 946, 1927, 2475 |
| BspDI | ATCGAT | 2 cut(s) | 427, 1794 |
| BspEI | TCCGGA | 1 cut(s) | 2636 |
| BspHI | TCATGA | 2 cut(s) | 261, 1863 |
| BspLI | GGNNCC | 4 cut(s) | 1638, 1734, 1978, 2833 |
| BspMAI | CTGCAG | 1 cut(s) | 1372 |
| BspPI | GGATC | 3 cut(s) | 76, 352, 2315 |
| BspT104I | TTCGAA | 2 cut(s) | 1198, 1351 |
| BspT107I | GGYRCC | 1 cut(s) | 1732 |
| BspTI | CTTAAG | 1 cut(s) | 1253 |
| BspTNI | GGTCTC | 2 cut(s) | 745, 1367 |
| BsrBI | CCGCTC | 1 cut(s) | 1858 |
| BsrDI | GCAATG | 2 cut(s) | 681, 2034 |
| BsrI | ACTGG | 2 cut(s) | 2353, 2463 |
| BssECI | CCNNGG | 7 cut(s) | 255, 1588, 1589, 1736, 1980, 2004, 2739 |
| BssSI | CACGAG | 1 cut(s) | 1289 |
| BssT1I | CCWWGG | 5 cut(s) | 255, 1736, 1980, 2004, 2739 |
| Bst2BI | CACGAG | 1 cut(s) | 1289 |
| Bst2UI | CCWGG | 3 cut(s) | 840, 1590, 2232 |
| Bst4CI | ACNGT | 8 cut(s) | 599, 739, 1013, 1841, 2210, 2262, 2665, 2857 |
| Bst6I | CTCTTC | 1 cut(s) | 2074 |
| BstAFI | CTTAAG | 1 cut(s) | 1253 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 251, 2126 |
| BstBAI | YACGTR | 1 cut(s) | 134 |
| BstBI | TTCGAA | 2 cut(s) | 1198, 1351 |
| BstC8I | GCNNGC | 3 cut(s) | 396, 577, 1927 |
| BstDSI | CCRYGG | 2 cut(s) | 1980, 2004 |
| BstEII | GGTNACC | 1 cut(s) | 1414 |
| BstF5I | GGATG | 3 cut(s) | 1609, 1885, 2014 |
| BstHHI | GCGC | 1 cut(s) | 607 |
| BstMAI | GTCTC | 6 cut(s) | 366, 745, 956, 1367, 2379, 2654 |
| BstMCI | CGRYCG | 1 cut(s) | 216 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 251, 776, 1127, 1340, 2126, 2170 |
| BstNI | CCWGG | 3 cut(s) | 840, 1590, 2232 |
| BstNSI | RCATGY | 3 cut(s) | 398, 979, 2404 |
| BstPI | GGTNACC | 1 cut(s) | 1414 |
| BstSCI | CCNGG | 4 cut(s) | 838, 1588, 2230, 2303 |
| BstSFI | CTRYAG | 3 cut(s) | 313, 846, 1368 |
| BstSLI | GKGCMC | 1 cut(s) | 172 |
| BstV1I | GCAGC | 4 cut(s) | 1130, 1568, 1834, 2176 |
| BstV2I | GAAGAC | 2 cut(s) | 138, 1182 |
| BstX2I | RGATCY | 2 cut(s) | 357, 1160 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 754 |
| BstYI | RGATCY | 2 cut(s) | 357, 1160 |
| Bsu15I | ATCGAT | 2 cut(s) | 427, 1794 |
| Bsu36I | CCTNAGG | 1 cut(s) | 173 |
| BsuRI | GGCC | 7 cut(s) | 770, 895, 959, 1594, 2245, 2462, 2849 |
| BsuTUI | ATCGAT | 2 cut(s) | 427, 1794 |
| BtgI | CCRYGG | 2 cut(s) | 1980, 2004 |
| BtgZI | GCGATG | 2 cut(s) | 317, 2848 |
| BtsCI | GGATG | 3 cut(s) | 1609, 1885, 2014 |
| BtsI | GCAGTG | 2 cut(s) | 507, 2046 |
| BtsIMutI | CAGTG | 8 cut(s) | 180, 394, 407, 507, 1585, 2046, 2215, 2470 |
| Cac8I | GCNNGC | 3 cut(s) | 396, 577, 1927 |
| CciI | TCATGA | 2 cut(s) | 261, 1863 |
| CfoI | GCGC | 1 cut(s) | 607 |
| Cfr13I | GGNCC | 1 cut(s) | 1592 |
| ClaI | ATCGAT | 2 cut(s) | 427, 1794 |
| CseI | GACGC | 1 cut(s) | 251 |
| Csp6I | GTAC | 9 cut(s) | 377, 1212, 1248, 1322, 1337, 1614, 1733, 2711, 2777 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2419, 2454 |
| CviQI | GTAC | 9 cut(s) | 377, 1212, 1248, 1322, 1337, 1614, 1733, 2711, 2777 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2729 |
| EaeI | YGGCCR | 4 cut(s) | 768, 893, 2460, 2847 |
| Eam1104I | CTCTTC | 1 cut(s) | 2074 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2729 |
| EarI | CTCTTC | 1 cut(s) | 2074 |
| Ecl136II | GAGCTC | 1 cut(s) | 2642 |
| Eco130I | CCWWGG | 5 cut(s) | 255, 1736, 1980, 2004, 2739 |
| Eco24I | GRGCYC | 1 cut(s) | 2644 |
| Eco31I | GGTCTC | 2 cut(s) | 745, 1367 |
| Eco53kI | GAGCTC | 1 cut(s) | 2642 |
| Eco57I | CTGAAG | 3 cut(s) | 210, 1350, 1740 |
| Eco72I | CACGTG | 1 cut(s) | 134 |
| Eco81I | CCTNAGG | 1 cut(s) | 173 |
| Eco88I | CYCGRG | 1 cut(s) | 2585 |
| Eco91I | GGTNACC | 1 cut(s) | 1414 |
| EcoICRI | GAGCTC | 1 cut(s) | 2642 |
| EcoO65I | GGTNACC | 1 cut(s) | 1414 |
| EcoRI | GAATTC | 4 cut(s) | 318, 365, 911, 2872 |
| EcoRII | CCWGG | 3 cut(s) | 838, 1588, 2230 |
| EcoT14I | CCWWGG | 5 cut(s) | 255, 1736, 1980, 2004, 2739 |
| EcoT22I | ATGCAT | 2 cut(s) | 231, 1804 |
| EcoT38I | GRGCYC | 1 cut(s) | 2644 |
| ErhI | CCWWGG | 5 cut(s) | 255, 1736, 1980, 2004, 2739 |
| Esp3I | CGTCTC | 3 cut(s) | 366, 2379, 2654 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1095, 1127 |
| FaqI | GGGAC | 6 cut(s) | 3, 191, 707, 740, 1058, 2669 |
| FauI | CCCGC | 1 cut(s) | 1396 |
| FbaI | TGATCA | 2 cut(s) | 487, 666 |
| FblI | GTMKAC | 4 cut(s) | 33, 680, 1539, 2859 |
| Fnu4HI | GCNGC | 7 cut(s) | 896, 1119, 1557, 1848, 2165, 2460, 2835 |
| FokI | GGATG | 3 cut(s) | 1616, 1872, 2021 |
| FriOI | GRGCYC | 1 cut(s) | 2644 |
| Fsp4HI | GCNGC | 7 cut(s) | 896, 1119, 1557, 1848, 2165, 2460, 2835 |
| FspI | TGCGCA | 1 cut(s) | 606 |
| GlaI | GCGC | 1 cut(s) | 606 |
| GluI | GCNGC | 7 cut(s) | 896, 1119, 1557, 1848, 2165, 2460, 2835 |
| GsuI | CTGGAG | 1 cut(s) | 2189 |
| HaeIII | GGCC | 7 cut(s) | 770, 895, 959, 1594, 2245, 2462, 2849 |
| HapII | CCGG | 3 cut(s) | 2305, 2637, 2718 |
| HgaI | GACGC | 1 cut(s) | 251 |
| HhaI | GCGC | 1 cut(s) | 607 |
| Hin6I | GCGC | 1 cut(s) | 605 |
| HinP1I | GCGC | 1 cut(s) | 605 |
| HincII | GTYRAC | 4 cut(s) | 34, 1945, 2350, 2811 |
| HindII | GTYRAC | 4 cut(s) | 34, 1945, 2350, 2811 |
| HindIII | AAGCTT | 2 cut(s) | 115, 1385 |
| HinfI | GANTC | 8 cut(s) | 1005, 1187, 1270, 1306, 1880, 2017, 2182, 2583 |
| HpaI | GTTAAC | 2 cut(s) | 2350, 2811 |
| HpaII | CCGG | 3 cut(s) | 2305, 2637, 2718 |
| HphI | GGTGA | 6 cut(s) | 284, 305, 735, 2204, 2542, 2707 |
| Hpy99I | CGWCG | 2 cut(s) | 1841, 2831 |
| HpyAV | CCTTC | 5 cut(s) | 754, 1194, 1404, 1439, 2387 |
| HpyCH4III | ACNGT | 8 cut(s) | 599, 739, 1013, 1841, 2210, 2262, 2665, 2857 |
| HpyCH4IV | ACGT | 5 cut(s) | 133, 375, 611, 2388, 2826 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 251, 776, 1127, 1340, 2126, 2170 |
| HpySE526I | ACGT | 5 cut(s) | 133, 375, 611, 2388, 2826 |
| HspAI | GCGC | 1 cut(s) | 605 |
| Kpn2I | TCCGGA | 1 cut(s) | 2636 |
| KpnI | GGTACC | 1 cut(s) | 1736 |
| Ksp22I | TGATCA | 2 cut(s) | 487, 666 |
| KspAI | GTTAAC | 2 cut(s) | 2350, 2811 |
| LmnI | GCTCC | 5 cut(s) | 1642, 1855, 2170, 2639, 2831 |
| Lsp1109I | GCAGC | 4 cut(s) | 1130, 1568, 1834, 2176 |
| LweI | GCATC | 6 cut(s) | 127, 175, 238, 515, 616, 1789 |
| MaeII | ACGT | 5 cut(s) | 133, 375, 611, 2388, 2826 |
| MbiI | CCGCTC | 1 cut(s) | 1858 |
| MfeI | CAATTG | 1 cut(s) | 1598 |
| MflI | RGATCY | 2 cut(s) | 357, 1160 |
| MhlI | GDGCHC | 3 cut(s) | 172, 558, 2644 |
| MlsI | TGGCCA | 2 cut(s) | 770, 2849 |
| MluNI | TGGCCA | 2 cut(s) | 770, 2849 |
| MlyI | GAGTC | 1 cut(s) | 2577 |
| MmeI | TCCRAC | 4 cut(s) | 447, 1888, 2179, 2809 |
| Mox20I | TGGCCA | 2 cut(s) | 770, 2849 |
| Mph1103I | ATGCAT | 2 cut(s) | 231, 1804 |
| MroI | TCCGGA | 1 cut(s) | 2636 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2337 |
| MscI | TGGCCA | 2 cut(s) | 770, 2849 |
| MslI | CAYNNNNRTG | 8 cut(s) | 62, 132, 452, 489, 1098, 1503, 2405, 2867 |
| Msp20I | TGGCCA | 2 cut(s) | 770, 2849 |
| MspA1I | CMGCKG | 2 cut(s) | 1403, 2167 |
| MspCI | CTTAAG | 1 cut(s) | 1253 |
| MspI | CCGG | 3 cut(s) | 2305, 2637, 2718 |
| MspR9I | CCNGG | 4 cut(s) | 840, 1590, 2232, 2305 |
| MunI | CAATTG | 1 cut(s) | 1598 |
| Mva1269I | GAATGC | 1 cut(s) | 2539 |
| MvaI | CCWGG | 3 cut(s) | 840, 1590, 2232 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 251, 776, 1127, 1340, 2126, 2170 |
| NciI | CCSGG | 1 cut(s) | 2305 |
| NcoI | CCATGG | 2 cut(s) | 1980, 2004 |
| NlaIV | GGNNCC | 4 cut(s) | 1638, 1734, 1978, 2833 |
| NmeAIII | GCCGAG | 1 cut(s) | 985 |
| NmuCI | GTSAC | 3 cut(s) | 916, 1723, 2548 |
| NsbI | TGCGCA | 1 cut(s) | 606 |
| NsiI | ATGCAT | 2 cut(s) | 231, 1804 |
| NspI | RCATGY | 3 cut(s) | 398, 979, 2404 |
| NspV | TTCGAA | 2 cut(s) | 1198, 1351 |
| PaeI | GCATGC | 1 cut(s) | 398 |
| PaeR7I | CTCGAG | 1 cut(s) | 2585 |
| PagI | TCATGA | 2 cut(s) | 261, 1863 |
| PasI | CCCWGGG | 1 cut(s) | 1589 |
| PciI | ACATGT | 1 cut(s) | 2400 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2592 |
| PctI | GAATGC | 1 cut(s) | 2539 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2337 |
| PfeI | GAWTC | 7 cut(s) | 1005, 1187, 1270, 1306, 1880, 2017, 2182 |
| PflFI | GACNNNGTC | 1 cut(s) | 1988 |
| PkrI | GCNGC | 7 cut(s) | 897, 1120, 1558, 1849, 2166, 2461, 2836 |
| Ple19I | CGATCG | 1 cut(s) | 216 |
| PleI | GAGTC | 1 cut(s) | 2577 |
| PmaCI | CACGTG | 1 cut(s) | 134 |
| PmlI | CACGTG | 1 cut(s) | 134 |
| PpsI | GAGTC | 1 cut(s) | 2577 |
| Ppu21I | YACGTR | 1 cut(s) | 134 |
| PscI | ACATGT | 1 cut(s) | 2400 |
| PshBI | ATTAAT | 2 cut(s) | 888, 2238 |
| Psp124BI | GAGCTC | 1 cut(s) | 2644 |
| Psp6I | CCWGG | 3 cut(s) | 838, 1588, 2230 |
| PspCI | CACGTG | 1 cut(s) | 134 |
| PspEI | GGTNACC | 1 cut(s) | 1414 |
| PspGI | CCWGG | 3 cut(s) | 838, 1588, 2230 |
| PspN4I | GGNNCC | 4 cut(s) | 1638, 1734, 1978, 2833 |
| PspPI | GGNCC | 1 cut(s) | 1592 |
| PspXI | VCTCGAGB | 1 cut(s) | 2585 |
| PstI | CTGCAG | 1 cut(s) | 1372 |
| PsuI | RGATCY | 2 cut(s) | 357, 1160 |
| PsyI | GACNNNGTC | 1 cut(s) | 1988 |
| PvuI | CGATCG | 1 cut(s) | 216 |
| PvuII | CAGCTG | 1 cut(s) | 2167 |
| RsaI | GTAC | 9 cut(s) | 378, 1213, 1249, 1323, 1338, 1615, 1734, 2712, 2778 |
| RsaNI | GTAC | 9 cut(s) | 377, 1212, 1248, 1322, 1337, 1614, 1733, 2711, 2777 |
| RseI | CAYNNNNRTG | 8 cut(s) | 62, 132, 452, 489, 1098, 1503, 2405, 2867 |
| SacI | GAGCTC | 1 cut(s) | 2644 |
| SalI | GTCGAC | 1 cut(s) | 32 |
| SatI | GCNGC | 7 cut(s) | 896, 1119, 1557, 1848, 2165, 2460, 2835 |
| Sau96I | GGNCC | 1 cut(s) | 1592 |
| SchI | GAGTC | 1 cut(s) | 2577 |
| ScrFI | CCNGG | 4 cut(s) | 840, 1590, 2232, 2305 |
| SduI | GDGCHC | 3 cut(s) | 172, 558, 2644 |
| SfaNI | GCATC | 6 cut(s) | 127, 175, 238, 515, 616, 1789 |
| SfcI | CTRYAG | 3 cut(s) | 313, 846, 1368 |
| Sfr274I | CTCGAG | 1 cut(s) | 2585 |
| SfuI | TTCGAA | 2 cut(s) | 1198, 1351 |
| SlaI | CTCGAG | 1 cut(s) | 2585 |
| SmiMI | CAYNNNNRTG | 8 cut(s) | 62, 132, 452, 489, 1098, 1503, 2405, 2867 |
| SmlI | CTYRAG | 7 cut(s) | 112, 813, 1253, 1820, 2247, 2585, 2804 |
| SmoI | CTYRAG | 7 cut(s) | 112, 813, 1253, 1820, 2247, 2585, 2804 |
| SpeI | ACTAGT | 1 cut(s) | 2435 |
| SphI | GCATGC | 1 cut(s) | 398 |
| SsiI | CCGC | 7 cut(s) | 235, 896, 1403, 1850, 1858, 2459, 2835 |
| SstI | GAGCTC | 1 cut(s) | 2644 |
| StyD4I | CCNGG | 4 cut(s) | 838, 1588, 2230, 2303 |
| StyI | CCWWGG | 5 cut(s) | 255, 1736, 1980, 2004, 2739 |
| TaaI | ACNGT | 8 cut(s) | 599, 739, 1013, 1841, 2210, 2262, 2665, 2857 |
| TaiI | ACGT | 5 cut(s) | 136, 378, 614, 2391, 2829 |
| TatI | WGTACW | 3 cut(s) | 1211, 1247, 1336 |
| TauI | GCSGC | 3 cut(s) | 898, 2462, 2837 |
| TfiI | GAWTC | 7 cut(s) | 1005, 1187, 1270, 1306, 1880, 2017, 2182 |
| TscAI | CASTG | 8 cut(s) | 187, 394, 407, 507, 1585, 2053, 2215, 2470 |
| TseFI | GTSAC | 3 cut(s) | 916, 1723, 2548 |
| TseI | GCWGC | 4 cut(s) | 1118, 1556, 1847, 2164 |
| Tsp45I | GTSAC | 3 cut(s) | 916, 1723, 2548 |
| TspGWI | ACGGA | 4 cut(s) | 744, 1661, 2164, 2387 |
| TspRI | CASTG | 8 cut(s) | 187, 394, 407, 507, 1585, 2053, 2215, 2470 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1988 |
| Vha464I | CTTAAG | 1 cut(s) | 1253 |
| VspI | ATTAAT | 2 cut(s) | 888, 2238 |
| XceI | RCATGY | 3 cut(s) | 398, 979, 2404 |
| XhoI | CTCGAG | 1 cut(s) | 2585 |
| XmaJI | CCTAGG | 1 cut(s) | 1736 |
| XmiI | GTMKAC | 4 cut(s) | 33, 680, 1539, 2859 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2337 |
| Zsp2I | ATGCAT | 2 cut(s) | 231, 1804 |