Prupe.1G197600_v2.0.a1

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000912
Physical Location & Seq
Reverse (-)
18890620 .. 18894752
4133 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G197600.1

Sequence Viewer

Length: 3123 bp
ATGAGCTCCTCCAATTGTGGCTGCCATATCTTCGACCTCAACACCTTTCAATCAATGGACTTTGGAGCCTCTTCGTCTTCTAGGGTTCTGAACAGCAACCAGCTCTCGTGCTTCAGCTATGTACCACCCAAATGCCAAAGGCATCTGGAGCCCTCAAGCCTTTACTGCCTTAATCATCCCAGAAATGCTTCCTTGGCACTCTCTCACAGAGATTGTGCTCTACGAGCCACCATTAAAGACACCAATGTCACCACGGAGAAGGTGAATGGTGATTGTTCTGCTGAAATCGAGCATTCGATTTTGACGGCGTTGTCCCCGTTGGACGGTCGTTATTGGGGTAAAGTCAAGGAATTAGCTCCTTATATGAGTGAATATGGGCTAATTTACTACCGCGTTCTAGTTGAGATCAAATGGTTGTTGAAACTATCACAAATTCTTGAAGTCACAGAGGTGCCCGGTTTCAGTGATGGTGCTCAGTCTTACTTGCAAGGAATTATAGATAGATTTTGCATCAGTGATGCATTAGAAATCAAGAGGATTGAGAAAATGACTAACCATGATGTAAAAGCAGTGGAGTACTTCTTGAAACAGAAATGCAGATCACATCCAGAAATAGCTAAGGTGCTTGAATTTTTTCATTTTGCTTGCACATCTGAGGACATCAACAATCTTGCTCATGCATTGATGTTAAAAGAAGCTATCAACAATGTTATATTTCCTGTCATGGACGATTTGATTGAGGCAATATGTGACATGTCAAAGGATAATGCTTCCATTCCCATGCTCTCTCGCACACATGGTCAGACAGCTTCACCCACAACTTTGGGGAAGGAAATGGCAATTTTTGCTGTGCGGCTAGGCATACAAAGGCACAGAATTTCTGAGGTCAAGATAATGGGGAAGTTTGCAGGTGCTGTAGGAAATTACAATGCTCATCTAGCTGCATATCCTAATATTAATTGGCCCCAAATTGCTGAAGAGTTTGTAACATCTCTTGGAGTGAGTTTTAATCATTATGTTACTCAGATCGAGACTCATGATTATATGTCTAAAGTTTTTAACGCATTCAACCGATTCAATAATATATTAATTGACTTTGATAGTGATATATGGCGCTACATATCTTTGGGTTACTTCAAGCAGACAACTAGGGCTGGTGAAATTGGCTCATCAACAATGCCTCACAAAGTAAACCCTATTGATTTTGAAAATAGTGAAGGTAATCTTGGTGTGGCTAGTGGAAGTCTTTCCTACTTGAGTGAGAAGTTGCCTAAATCGCGTTTGCAGCGTGACCTGACTGATTCTACTGTTCTGAGGAACATGGGTGTTGGACTAGGACACTCTCTTCTTGCTTACAGAAGCACACTGCAGGGAATATCAAAGCTTCAGGTTAATGAAGCTCGCATAAGTGAGGAGTTGAACCAATCTTGGGAGGTGCTTGCTGAAGCAATACAAACTGTTATGCGAAGATATAGTGTTCCTGAGCCTTATGAGAAGCTGAAGGAACTAACAAGAGGAAGAACAGTTACCAAAGACAGAATAAGAGAGTTCATTAAAGGCTTGGAATTACCTGAGGAACCAAAGATCATTTTATCAAATCTGACGCCGCATAGCTATGTTGGAGCAGCAGTTAAACTGGCCAGGATGGTAGACACGGCTGTTAGATCCACCAGAAAAAACACCAATGTTTCCACTGAGAAGGTGAAGATGGTGTCTGGCAACTCTTCTAGTGAATCTGAGCTTGTGAATTTGATGGCTTTGTCACCATTGGATGGTCGGTATTGGGGTAAAGTCAAGGACTTGGCTCCTTACATGAGTGAGTATGGCCTGATCTACTTCCGTGTTCTAGTTGAGATCAAATGGTTGCTATGGCTTTCACAAATTCCTGAAGTCACAGAGGTACCCACCTTCAGTGAAAATGCTCGGTCTTATTTGCAAGAAATGATTGATGGATTTAGTTACAATAATGCATTAGAAATAAAGAAGATTGAGAAAGTGACAAATCATGATGTGAAAGCGGTGGAGTACTTCTTGAAACAGAGATTCCAATCACATCCAGAAATAGCCAAGGTGCTGGAATTTTTTCATTTTGCTTGCACATCCGAGGACATCAACAATCTTGCCCATGCATTGATGTTGAAAGAAGCCGTGAACAGTGTCATATATCCTGTCATGGATGATTTGGTTGAGGCAGTATGTAACATGGCCAAGGACAATGCTCATATTTCTATGCTCTCTCGTACTCATGGACAGCCTGCTTCACCCACAACTTTGGGAAAGGAAATGGCAAATTTTGCAGTCAGATTAAGCAGAGAAAGGCACGAGATCTCTCGGGTGGAAATAATGGGAAAATTTGCTGGTGCTGTTGGAAATTACAATGCTCATCTTGTCGCATATCCTGATATTAATTGGCCCCAAATTGCTGAAGAGTTTGTAACATCTCTTGGGTTGAGTTTTAATCCCTATGTTACACAGATTGAACCTCATGACTATATGGCAGAACTTTTTCATGCATTTAGCCAGTTCAACAATATATTGATTGACTTTGATAGAGATATATGGGACTACATATCTTTGGGATATTTTAAGCAGACAACAAAGGCTGGTGAGATTGGGTCTTCAACAATGCCTCACAAGGTAAACCCTATTGATTTTGAAAACAGTGAAGGTAACCTTGGTGTGGCTAATGGCAGTTTTTATCATCTGAGCATGAAGTTGCCTATTTCGCGTTGGCAGCGTGACTTGACGGATTCAACTGTTTTAAGGAACATGGGTTTAGGATTAGGGCATTCTCTTCTTGCCTACAAAAGCACACTGCAGGGAATATCAAAGCTTCAGGTCAATGAAGGTTGCATAAGTGAGGACTTGAACCTTACTTGGGAAGTTCTTGCTGAACCAATACAAACAGTTATGCGAAGATATGGTGTTCCAGAGCCCTATGAAAAGTTGAAGGAACTAACCAGAGGAAGAGCAGTAACCAAGGAAAGTATAATAGATTTTATGCAAGATTTGGAGTTACCTAACGAAGCAAAGTCCAATCTACTAAAGTTAACACCGCATAGTTATGTTGGAGCAGCTGTTGAATTGGCTAGGACGGTAGACATAGCCGTGAAGGTGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

1041

Amino Acids

117.25

Weight (kDa)

6.11

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 899
AasI GACNNNNNNGTC 2 cut(s) 245, 310
Acc36I ACCTGC 1 cut(s) 899
Acc65I GGTACC 1 cut(s) 1900
AccB1I GGYRCC 2 cut(s) 451, 1900
AccB7I CCANNNNNTGG 1 cut(s) 1772
AccI GTMKAC 2 cut(s) 1650, 3099
AccII CGCG 3 cut(s) 393, 1279, 2728
AciI CCGC 5 cut(s) 391, 853, 1607, 2018, 3056
AclWI GGATC 1 cut(s) 1659
AcoI YGGCCR 2 cut(s) 1638, 2205
AcsI RAATTY 8 cut(s) 432, 629, 876, 1747, 1881, 2078, 2290, 2351
AcuI CTGAAG 9 cut(s) 97, 996, 1370, 1464, 1520, 1894, 1908, 2445, 2819
AcyI GRCGYC 1 cut(s) 1604
AfaI GTAC 5 cut(s) 123, 578, 1902, 2027, 2242
AfiI CCNNNNNNNGG 5 cut(s) 323, 1429, 1772, 2680, 2878
AflIII ACRYGT 1 cut(s) 753
AjnI CCWGG 1 cut(s) 1640
AjuI GAANNNNNNNTTGG 4 cut(s) 1209, 1241, 2658, 2690
AleI CACNNNNGTG 1 cut(s) 449
Alw21I GWGCWC 3 cut(s) 8, 220, 475
Alw26I GTCTC 1 cut(s) 1025
AlwI GGATC 1 cut(s) 1659
AlwNI CAGNNNCTG 1 cut(s) 914
Ama87I CYCGRG 1 cut(s) 2331
AoxI GGCC 5 cut(s) 962, 1638, 1825, 2205, 2411
ApeKI GCWGC 6 cut(s) 21, 941, 1285, 1625, 2734, 3074
ApoI RAATTY 8 cut(s) 432, 629, 876, 1747, 1881, 2078, 2290, 2351
AseI ATTAAT 3 cut(s) 957, 1088, 2406
Asp700I GAANNNNTTC 5 cut(s) 187, 633, 1246, 2082, 2505
Asp718I GGTACC 1 cut(s) 1900
AspLEI GCGC 1 cut(s) 1116
AspS9I GGNCC 2 cut(s) 963, 2412
AsuC2I CCSGG 1 cut(s) 456
AsuHPI GGTGA 9 cut(s) 241, 274, 281, 804, 1169, 1714, 1755, 2253, 2618
AvaI CYCGRG 1 cut(s) 2331
AxyI CCTNAGG 1 cut(s) 1572
BaeGI GKGCMC 1 cut(s) 456
BaeI ACNNNNGTAYC 2 cut(s) 1884, 1917
BalI TGGCCA 2 cut(s) 1640, 2207
BanI GGYRCC 2 cut(s) 451, 1900
BanII GRGCYC 3 cut(s) 8, 153, 2937
BarI GAAGNNNNNNTAC 2 cut(s) 1510, 1542
BauI CACGAG 2 cut(s) 106, 2321
BbsI GAAGAC 2 cut(s) 69, 2610
Bbv12I GWGCWC 3 cut(s) 8, 220, 475
BbvI GCAGC 6 cut(s) 8, 928, 1297, 1637, 2746, 3086
BccI CCATC 6 cut(s) 461, 1639, 1702, 1747, 1766, 1943
BceAI ACGGC 4 cut(s) 321, 1671, 2132, 3092
BciT130I CCWGG 1 cut(s) 1642
BcnI CCSGG 1 cut(s) 456
BcoDI GTCTC 1 cut(s) 1025
BfmI CTRYAG 3 cut(s) 915, 1367, 2816
BfoI RGCGCY 1 cut(s) 1117
BfuAI ACCTGC 1 cut(s) 899
BglII AGATCT 1 cut(s) 2325
BisI GCNGC 8 cut(s) 22, 854, 942, 1286, 1607, 1626, 2735, 3075
BlsI GCNGC 8 cut(s) 23, 855, 943, 1287, 1608, 1627, 2736, 3076
BmcAI AGTACT 2 cut(s) 578, 2027
Bme1390I CCNGG 2 cut(s) 456, 1642
BmeT110I CYCGRG 1 cut(s) 2331
BmgT120I GGNCC 2 cut(s) 963, 2412
BmiI GGNNCC 8 cut(s) 67, 150, 453, 965, 1578, 1806, 1902, 2414
BmrFI CCNGG 2 cut(s) 456, 1642
BmsI GCATC 3 cut(s) 151, 508, 519
BpiI GAAGAC 2 cut(s) 69, 2610
BpmI CTGGAG 1 cut(s) 167
Bpu10I CCTNAGC 2 cut(s) 618, 1482
BpuEI CTTGAG 2 cut(s) 139, 1276
BpuMI CCSGG 1 cut(s) 456
BsaBI GATNNNNATC 1 cut(s) 2047
BsaHI GRCGYC 1 cut(s) 1604
BsaJI CCNNGG 7 cut(s) 192, 252, 2067, 2103, 2208, 2674, 2979
BsaXI ACNNNNNCTCC 4 cut(s) 1614, 1644, 3063, 3093
Bsc4I CCNNNNNNNGG 5 cut(s) 323, 1429, 1772, 2680, 2878
Bse1I ACTGG 2 cut(s) 1641, 2521
Bse21I CCTNAGG 1 cut(s) 1572
Bse8I GATNNNNATC 1 cut(s) 2047
BseBI CCWGG 1 cut(s) 1642
BseDI CCNNGG 7 cut(s) 192, 252, 2067, 2103, 2208, 2674, 2979
BseGI GGATG 7 cut(s) 175, 604, 1650, 1777, 2053, 2099, 2182
BseJI GATNNNNATC 1 cut(s) 2047
BseLI CCNNNNNNNGG 5 cut(s) 323, 1429, 1772, 2680, 2878
BseNI ACTGG 2 cut(s) 1641, 2521
BseRI GAGGAG 1 cut(s) 1427
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 6 cut(s) 8, 928, 1297, 1637, 2746, 3086
Bsh1236I CGCG 3 cut(s) 393, 1279, 2728
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 5 cut(s) 964, 1640, 1827, 2207, 2413
BshNI GGYRCC 2 cut(s) 451, 1900
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 3 cut(s) 8, 220, 475
BsiHKCI CYCGRG 1 cut(s) 2331
BsiSI CCGG 1 cut(s) 456
BslFI GGGAC 2 cut(s) 298, 2576
BslI CCNNNNNNNGG 5 cut(s) 323, 1429, 1772, 2680, 2878
BsmAI GTCTC 1 cut(s) 1025
BsmFI GGGAC 2 cut(s) 298, 2576
BsmI GAATGC 3 cut(s) 292, 1064, 2788
BsnI GGCC 5 cut(s) 964, 1640, 1827, 2207, 2413
BsoBI CYCGRG 1 cut(s) 2331
Bsp1286I GDGCHC 6 cut(s) 8, 153, 220, 456, 475, 2937
Bsp143I GATC 8 cut(s) 405, 599, 1026, 1584, 1664, 1830, 1854, 2325
BspACI CCGC 5 cut(s) 391, 853, 1607, 2018, 3056
BspANI GGCC 5 cut(s) 964, 1640, 1827, 2207, 2413
BspFNI CGCG 3 cut(s) 393, 1279, 2728
BspHI TCATGA 3 cut(s) 1036, 2005, 2485
BspLI GGNNCC 8 cut(s) 67, 150, 453, 965, 1578, 1806, 1902, 2414
BspMAI CTGCAG 2 cut(s) 1371, 2820
BspMI ACCTGC 1 cut(s) 899
BspPI GGATC 1 cut(s) 1659
BspQI GCTCTTC 1 cut(s) 2962
BspT107I GGYRCC 2 cut(s) 451, 1900
BsrI ACTGG 2 cut(s) 1641, 2521
BssECI CCNNGG 7 cut(s) 192, 252, 2067, 2103, 2208, 2674, 2979
BssMI GATC 8 cut(s) 405, 599, 1026, 1584, 1664, 1830, 1854, 2325
BssNI GRCGYC 1 cut(s) 1604
BssSI CACGAG 2 cut(s) 106, 2321
BssT1I CCWWGG 5 cut(s) 192, 2067, 2208, 2674, 2979
Bst2BI CACGAG 2 cut(s) 106, 2321
Bst2UI CCWGG 1 cut(s) 1642
Bst4CI ACNGT 9 cut(s) 326, 1309, 1459, 1525, 2156, 2663, 2758, 2908, 3097
Bst6I CTCTTC 7 cut(s) 76, 972, 1350, 1729, 2421, 2799, 2962
BstACI GRCGYC 1 cut(s) 1604
BstAPI GCANNNNNTGC 2 cut(s) 845, 2294
BstC8I GCNNGC 5 cut(s) 646, 1402, 1440, 2095, 2256
BstDSI CCRYGG 1 cut(s) 252
BstEII GGTNACC 1 cut(s) 2669
BstF5I GGATG 7 cut(s) 175, 604, 1650, 1777, 2053, 2099, 2182
BstFNI CGCG 3 cut(s) 393, 1279, 2728
BstH2I RGCGCY 1 cut(s) 1117
BstHHI GCGC 1 cut(s) 1116
BstKTI GATC 8 cut(s) 408, 602, 1029, 1587, 1667, 1833, 1857, 2328
BstMAI GTCTC 1 cut(s) 1025
BstMBI GATC 8 cut(s) 405, 599, 1026, 1584, 1664, 1830, 1854, 2325
BstMCI CGRYCG 1 cut(s) 328
BstNI CCWGG 1 cut(s) 1642
BstNSI RCATGY 1 cut(s) 757
BstPI GGTNACC 1 cut(s) 2669
BstSCI CCNGG 2 cut(s) 454, 1640
BstSFI CTRYAG 3 cut(s) 915, 1367, 2816
BstSLI GKGCMC 1 cut(s) 456
BstUI CGCG 3 cut(s) 393, 1279, 2728
BstV1I GCAGC 6 cut(s) 8, 928, 1297, 1637, 2746, 3086
BstV2I GAAGAC 2 cut(s) 69, 2610
BstX2I RGATCY 2 cut(s) 1664, 2325
BstXI CCANNNNNNTGG 3 cut(s) 823, 2074, 2272
BstYI RGATCY 2 cut(s) 1664, 2325
Bsu36I CCTNAGG 1 cut(s) 1572
BsuRI GGCC 5 cut(s) 964, 1640, 1827, 2207, 2413
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 7 cut(s) 175, 604, 1650, 1777, 2053, 2099, 2182
BtsI GCAGTG 3 cut(s) 576, 1364, 2813
BtsIMutI CAGTG 9 cut(s) 469, 520, 576, 1364, 1692, 1918, 2161, 2668, 2813
BveI ACCTGC 1 cut(s) 899
Cac8I GCNNGC 5 cut(s) 646, 1402, 1440, 2095, 2256
CaiI CAGNNNCTG 1 cut(s) 914
CciI TCATGA 3 cut(s) 1036, 2005, 2485
CfoI GCGC 1 cut(s) 1116
Cfr13I GGNCC 2 cut(s) 963, 2412
CseI GACGC 1 cut(s) 1612
Csp6I GTAC 5 cut(s) 122, 577, 1901, 2026, 2241
CviQI GTAC 5 cut(s) 122, 577, 1901, 2026, 2241
DpnI GATC 8 cut(s) 407, 601, 1028, 1586, 1666, 1832, 1856, 2327
DpnII GATC 8 cut(s) 405, 599, 1026, 1584, 1664, 1830, 1854, 2325
DrdI GACNNNNNNGTC 2 cut(s) 245, 310
DseDI GACNNNNNNGTC 2 cut(s) 245, 310
EaeI YGGCCR 2 cut(s) 1638, 2205
Eam1104I CTCTTC 7 cut(s) 76, 972, 1350, 1729, 2421, 2799, 2962
EarI CTCTTC 7 cut(s) 76, 972, 1350, 1729, 2421, 2799, 2962
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 5 cut(s) 192, 2067, 2208, 2674, 2979
Eco24I GRGCYC 3 cut(s) 8, 153, 2937
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 9 cut(s) 97, 996, 1370, 1464, 1520, 1894, 1908, 2445, 2819
Eco81I CCTNAGG 1 cut(s) 1572
Eco88I CYCGRG 1 cut(s) 2331
Eco91I GGTNACC 1 cut(s) 2669
EcoICRI GAGCTC 1 cut(s) 6
EcoO65I GGTNACC 1 cut(s) 2669
EcoRII CCWGG 1 cut(s) 1640
EcoT14I CCWWGG 5 cut(s) 192, 2067, 2208, 2674, 2979
EcoT22I ATGCAT 5 cut(s) 523, 682, 1972, 2131, 2515
EcoT38I GRGCYC 3 cut(s) 8, 153, 2937
ErhI CCWWGG 5 cut(s) 192, 2067, 2208, 2674, 2979
FalI AAGNNNNNCTT 4 cut(s) 1209, 1241, 2658, 2690
FaqI GGGAC 2 cut(s) 298, 2576
FblI GTMKAC 2 cut(s) 1650, 3099
Fnu4HI GCNGC 8 cut(s) 22, 854, 942, 1286, 1607, 1626, 2735, 3075
FokI GGATG 7 cut(s) 162, 591, 1657, 1784, 2040, 2086, 2189
FriOI GRGCYC 3 cut(s) 8, 153, 2937
Fsp4HI GCNGC 8 cut(s) 22, 854, 942, 1286, 1607, 1626, 2735, 3075
GlaI GCGC 1 cut(s) 1115
GluI GCNGC 8 cut(s) 22, 854, 942, 1286, 1607, 1626, 2735, 3075
GsuI CTGGAG 1 cut(s) 167
HaeII RGCGCY 1 cut(s) 1117
HaeIII GGCC 5 cut(s) 964, 1640, 1827, 2207, 2413
HapII CCGG 1 cut(s) 456
HgaI GACGC 1 cut(s) 1612
HhaI GCGC 1 cut(s) 1116
Hin1I GRCGYC 1 cut(s) 1604
Hin6I GCGC 1 cut(s) 1114
HinP1I GCGC 1 cut(s) 1114
HincII GTYRAC 1 cut(s) 3051
HindII GTYRAC 1 cut(s) 3051
HindIII AAGCTT 2 cut(s) 1382, 2831
HinfI GANTC 6 cut(s) 1033, 1074, 1301, 1733, 2043, 2750
HpaI GTTAAC 1 cut(s) 3051
HpaII CCGG 1 cut(s) 456
HphI GGTGA 9 cut(s) 241, 274, 281, 804, 1169, 1714, 1755, 2253, 2618
Hpy166II GTNNAC 6 cut(s) 1192, 1651, 2152, 2641, 3051, 3100
Hpy8I GTNNAC 6 cut(s) 1192, 1651, 2152, 2641, 3051, 3100
HpyCH4III ACNGT 9 cut(s) 326, 1309, 1459, 1525, 2156, 2663, 2758, 2908, 3097
Hsp92I GRCGYC 1 cut(s) 1604
HspAI GCGC 1 cut(s) 1114
KpnI GGTACC 1 cut(s) 1904
KspAI GTTAAC 1 cut(s) 3051
Kzo9I GATC 8 cut(s) 405, 599, 1026, 1584, 1664, 1830, 1854, 2325
LguI GCTCTTC 1 cut(s) 2962
LmnI GCTCC 7 cut(s) 11, 65, 148, 361, 1622, 1810, 3071
Lsp1109I GCAGC 6 cut(s) 8, 928, 1297, 1637, 2746, 3086
LweI GCATC 3 cut(s) 151, 508, 519
MalI GATC 8 cut(s) 407, 601, 1028, 1586, 1666, 1832, 1856, 2327
MboI GATC 8 cut(s) 405, 599, 1026, 1584, 1664, 1830, 1854, 2325
MfeI CAATTG 1 cut(s) 13
MflI RGATCY 2 cut(s) 1664, 2325
MhlI GDGCHC 6 cut(s) 8, 153, 220, 456, 475, 2937
MlsI TGGCCA 2 cut(s) 1640, 2207
MluNI TGGCCA 2 cut(s) 1640, 2207
MlyI GAGTC 1 cut(s) 1027
MmeI TCCRAC 5 cut(s) 300, 1309, 1600, 2347, 3049
Mox20I TGGCCA 2 cut(s) 1640, 2207
Mph1103I ATGCAT 5 cut(s) 523, 682, 1972, 2131, 2515
MroXI GAANNNNTTC 5 cut(s) 187, 633, 1246, 2082, 2505
MscI TGGCCA 2 cut(s) 1640, 2207
MslI CAYNNNNRTG 6 cut(s) 130, 449, 779, 1614, 3063, 3107
Msp20I TGGCCA 2 cut(s) 1640, 2207
MspA1I CMGCKG 1 cut(s) 3077
MspI CCGG 1 cut(s) 456
MspR9I CCNGG 2 cut(s) 456, 1642
MunI CAATTG 1 cut(s) 13
Mva1269I GAATGC 3 cut(s) 292, 1064, 2788
MvaI CCWGG 1 cut(s) 1642
MvnI CGCG 3 cut(s) 393, 1279, 2728
NciI CCSGG 1 cut(s) 456
NdeII GATC 8 cut(s) 405, 599, 1026, 1584, 1664, 1830, 1854, 2325
NlaIV GGNNCC 8 cut(s) 67, 150, 453, 965, 1578, 1806, 1902, 2414
NmuCI GTSAC 8 cut(s) 247, 442, 749, 1289, 1761, 1891, 1996, 2738
NsiI ATGCAT 5 cut(s) 523, 682, 1972, 2131, 2515
NspI RCATGY 1 cut(s) 757
OliI CACNNNNGTG 1 cut(s) 449
PagI TCATGA 3 cut(s) 1036, 2005, 2485
PaqCI CACCTGC 1 cut(s) 899
PciI ACATGT 1 cut(s) 753
PciSI GCTCTTC 1 cut(s) 2962
PctI GAATGC 3 cut(s) 292, 1064, 2788
PdmI GAANNNNTTC 5 cut(s) 187, 633, 1246, 2082, 2505
PfeI GAWTC 5 cut(s) 1074, 1301, 1733, 2043, 2750
PflMI CCANNNNNTGG 1 cut(s) 1772
PkrI GCNGC 8 cut(s) 23, 855, 943, 1287, 1608, 1627, 2736, 3076
PleI GAGTC 1 cut(s) 1027
PpsI GAGTC 1 cut(s) 1027
PscI ACATGT 1 cut(s) 753
PshBI ATTAAT 3 cut(s) 957, 1088, 2406
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 1640
PspEI GGTNACC 1 cut(s) 2669
PspGI CCWGG 1 cut(s) 1640
PspN4I GGNNCC 8 cut(s) 67, 150, 453, 965, 1578, 1806, 1902, 2414
PspPI GGNCC 2 cut(s) 963, 2412
PstI CTGCAG 2 cut(s) 1371, 2820
PstNI CAGNNNCTG 1 cut(s) 914
PsuI RGATCY 2 cut(s) 1664, 2325
PvuII CAGCTG 1 cut(s) 3077
RsaI GTAC 5 cut(s) 123, 578, 1902, 2027, 2242
RsaNI GTAC 5 cut(s) 122, 577, 1901, 2026, 2241
RseI CAYNNNNRTG 6 cut(s) 130, 449, 779, 1614, 3063, 3107
SacI GAGCTC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 2962
SatI GCNGC 8 cut(s) 22, 854, 942, 1286, 1607, 1626, 2735, 3075
Sau3AI GATC 8 cut(s) 405, 599, 1026, 1584, 1664, 1830, 1854, 2325
Sau96I GGNCC 2 cut(s) 963, 2412
ScaI AGTACT 2 cut(s) 578, 2027
SchI GAGTC 1 cut(s) 1027
ScrFI CCNGG 2 cut(s) 456, 1642
SduI GDGCHC 6 cut(s) 8, 153, 220, 456, 475, 2937
SfaNI GCATC 3 cut(s) 151, 508, 519
SfcI CTRYAG 3 cut(s) 915, 1367, 2816
SmiMI CAYNNNNRTG 6 cut(s) 130, 449, 779, 1614, 3063, 3107
SmlI CTYRAG 2 cut(s) 154, 1255
SmoI CTYRAG 2 cut(s) 154, 1255
SsiI CCGC 5 cut(s) 391, 853, 1607, 2018, 3056
SspI AATATT 1 cut(s) 955
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 454, 1640
StyI CCWWGG 5 cut(s) 192, 2067, 2208, 2674, 2979
TaaI ACNGT 9 cut(s) 326, 1309, 1459, 1525, 2156, 2663, 2758, 2908, 3097
TaqI TCGA 4 cut(s) 33, 288, 296, 1029
TaqII GACCGA 1 cut(s) 1914
TatI WGTACW 2 cut(s) 576, 2025
TauI GCSGC 2 cut(s) 856, 1609
TfiI GAWTC 5 cut(s) 1074, 1301, 1733, 2043, 2750
TscAI CASTG 9 cut(s) 469, 520, 576, 1371, 1699, 1918, 2161, 2668, 2820
TseFI GTSAC 8 cut(s) 247, 442, 749, 1289, 1761, 1891, 1996, 2738
TseI GCWGC 6 cut(s) 21, 941, 1285, 1625, 2734, 3074
Tsp45I GTSAC 8 cut(s) 247, 442, 749, 1289, 1761, 1891, 1996, 2738
TspDTI ATGAA 8 cut(s) 626, 1410, 1540, 2075, 2498, 2726, 2859, 2955
TspGWI ACGGA 3 cut(s) 269, 1829, 2762
TspRI CASTG 9 cut(s) 469, 520, 576, 1371, 1699, 1918, 2161, 2668, 2820
Van91I CCANNNNNTGG 1 cut(s) 1772
VspI ATTAAT 3 cut(s) 957, 1088, 2406
XapI RAATTY 8 cut(s) 432, 629, 876, 1747, 1881, 2078, 2290, 2351
XceI RCATGY 1 cut(s) 757
XmiI GTMKAC 2 cut(s) 1650, 3099
XmnI GAANNNNTTC 5 cut(s) 187, 633, 1246, 2082, 2505
ZrmI AGTACT 2 cut(s) 578, 2027
Zsp2I ATGCAT 5 cut(s) 523, 682, 1972, 2131, 2515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.