RchiOBHm_Chr5g0043781

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000912
Physical Location & Seq
Forward (+)
38821293 .. 38822607
1315 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32201

Sequence Viewer

Length: 651 bp
ATGCGAGTGCTTGAATTTTTCCATTTTGCTTGCACATCTGAGGATATCAACAATCTTGCCCATGCATTAATGCTAGAAGGAGCCATGAACAATGTCGTACTCCCTTTCATGGATGATTTGATTCAGGCTCTATGTAACATGGCCAAGGACAATGCTCATATTCCTAGGGGGTCTCGTACAATGCTCATATTTAGGCTTCTTGCATGTACTGAAATTCCTTTATCCTTGCTTGATGCACCAACTGTGGGGAAAGAAATGGCAACTTTTGCTGTGAGATTAGGAGGGAAAGTTTCTGGTGCTTTTGGAAATTACAACACTCATCTTATAACTAAGGACGGTGAGGTTGGTTCATCAACAATGCCTCAAAAAGTAAACCCTATTGATTTTGAAAACAGCAAAGGCAATCTTGGTGTGGCTAATGCCAATCTTTATCACCCGAGCATGAAGTTGCCTATTTCATGTTTGCAGCTAAAAAGAACTTCAGTTTTCCTTTCAACTTGTTTCTGTTCCCTACAAGTCCTTAAGCGAGACTTGATCGACTCATCTGTTTTGAGGAACATGGATTTAGGATTAAGGCATTCTATTCTTGCCTACAAAAGCACACTTCAGGGAATATCAAAGCTTCAGGTATGCAGCACATACAATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

216

Amino Acids

23.76

Weight (kDa)

8.48

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 5 - 62 4.4e-07 Lyase
Lyase_1 PF00206 95 - 135 7.1e-07 Lyase
ASL_C PF08328 174 - 211 3.2e-08 Adenylosuccinate lyase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 2 cut(s) 14, 213
AcuI CTGAAG 3 cut(s) 465, 590, 608
AfaI GTAC 3 cut(s) 99, 178, 208
AfiI CCNNNNNNNGG 2 cut(s) 109, 245
AflII CTTAAG 1 cut(s) 521
AgsI TTSAA 3 cut(s) 14, 389, 495
AluBI AGCT 2 cut(s) 469, 622
AluI AGCT 2 cut(s) 469, 622
Alw26I GTCTC 2 cut(s) 177, 522
Ama87I CYCGRG 1 cut(s) 436
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 466, 633
ApoI RAATTY 2 cut(s) 14, 213
AseI ATTAAT 1 cut(s) 68
AspA2I CCTAGG 1 cut(s) 164
AsuHPI GGTGA 2 cut(s) 350, 425
AvaI CYCGRG 1 cut(s) 436
AvrII CCTAGG 1 cut(s) 164
BalI TGGCCA 1 cut(s) 143
BbvI GCAGC 2 cut(s) 478, 645
BcoDI GTCTC 2 cut(s) 177, 522
BfaI CTAG 2 cut(s) 74, 165
BfrI CTTAAG 1 cut(s) 521
BisI GCNGC 2 cut(s) 467, 634
BlnI CCTAGG 1 cut(s) 164
BlsI GCNGC 2 cut(s) 468, 635
BmeT110I CYCGRG 1 cut(s) 436
BmiI GGNNCC 1 cut(s) 82
BmsI GCATC 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 144, 164
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 245
BseDI CCNNGG 2 cut(s) 144, 164
BseGI GGATG 1 cut(s) 118
BseLI CCNNNNNNNGG 2 cut(s) 109, 245
BseMII CTCAG 1 cut(s) 30
BseXI GCAGC 2 cut(s) 478, 645
BshFI GGCC 1 cut(s) 143
BsiHKCI CYCGRG 1 cut(s) 436
BslI CCNNNNNNNGG 2 cut(s) 109, 245
BsmAI GTCTC 2 cut(s) 177, 522
BsmI GAATGC 1 cut(s) 577
BsnI GGCC 1 cut(s) 143
Bso31I GGTCTC 1 cut(s) 177
BsoBI CYCGRG 1 cut(s) 436
Bsp143I GATC 1 cut(s) 534
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 1 cut(s) 31
BspLI GGNNCC 1 cut(s) 82
BspTI CTTAAG 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 144, 164
BssMI GATC 1 cut(s) 534
BssT1I CCWWGG 2 cut(s) 144, 164
Bst4CI ACNGT 2 cut(s) 244, 338
BstAFI CTTAAG 1 cut(s) 521
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 2 cut(s) 39, 330
BstF5I GGATG 1 cut(s) 118
BstKTI GATC 1 cut(s) 537
BstMAI GTCTC 2 cut(s) 177, 522
BstMBI GATC 1 cut(s) 534
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstNSI RCATGY 1 cut(s) 207
BstV1I GCAGC 2 cut(s) 478, 645
BsuRI GGCC 1 cut(s) 143
BtsCI GGATG 1 cut(s) 118
Cac8I GCNNGC 1 cut(s) 31
Csp6I GTAC 3 cut(s) 98, 177, 207
CviAII CATG 8 cut(s) 62, 85, 109, 139, 204, 442, 459, 559
CviJI RGCY 7 cut(s) 83, 128, 143, 196, 416, 469, 622
CviKI_1 RGCY 7 cut(s) 83, 128, 143, 196, 416, 469, 622
CviQI GTAC 3 cut(s) 98, 177, 207
DdeI CTNAG 2 cut(s) 39, 330
DpnI GATC 1 cut(s) 536
DpnII GATC 1 cut(s) 534
EaeI YGGCCR 1 cut(s) 141
Eco130I CCWWGG 2 cut(s) 144, 164
Eco31I GGTCTC 1 cut(s) 177
Eco32I GATATC 1 cut(s) 46
Eco57I CTGAAG 3 cut(s) 465, 590, 608
Eco88I CYCGRG 1 cut(s) 436
EcoRV GATATC 1 cut(s) 46
EcoT14I CCWWGG 2 cut(s) 144, 164
EcoT22I ATGCAT 1 cut(s) 67
ErhI CCWWGG 2 cut(s) 144, 164
FaeI CATG 8 cut(s) 65, 88, 112, 142, 207, 445, 462, 562
FalI AAGNNNNNCTT 4 cut(s) 390, 422, 515, 547
FatI CATG 8 cut(s) 61, 84, 108, 138, 203, 441, 458, 558
Fnu4HI GCNGC 2 cut(s) 467, 634
FokI GGATG 1 cut(s) 125
Fsp4HI GCNGC 2 cut(s) 467, 634
FspBI CTAG 2 cut(s) 74, 165
GluI GCNGC 2 cut(s) 467, 634
HaeIII GGCC 1 cut(s) 143
Hin1II CATG 8 cut(s) 65, 88, 112, 142, 207, 445, 462, 562
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 2 cut(s) 121, 539
HphI GGTGA 2 cut(s) 350, 425
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 1 cut(s) 40
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 2 cut(s) 244, 338
HpyCH4V TGCA 6 cut(s) 33, 65, 203, 236, 466, 633
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 2 cut(s) 39, 330
Hsp92II CATG 8 cut(s) 65, 88, 112, 142, 207, 445, 462, 562
Kzo9I GATC 1 cut(s) 534
LmnI GCTCC 1 cut(s) 80
LpnPI CCDG 4 cut(s) 110, 279, 593, 611
Lsp1109I GCAGC 2 cut(s) 478, 645
LweI GCATC 1 cut(s) 223
MaeI CTAG 2 cut(s) 74, 165
MaeIII GTNAC 1 cut(s) 134
MalI GATC 1 cut(s) 536
MboI GATC 1 cut(s) 534
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 3 cut(s) 14, 213, 307
MluNI TGGCCA 1 cut(s) 143
MlyI GAGTC 1 cut(s) 533
MnlI CCTC 5 cut(s) 34, 275, 334, 372, 546
Mox20I TGGCCA 1 cut(s) 143
Mph1103I ATGCAT 1 cut(s) 67
MscI TGGCCA 1 cut(s) 143
MseI TTAA 3 cut(s) 68, 522, 572
Msp20I TGGCCA 1 cut(s) 143
MspCI CTTAAG 1 cut(s) 521
Mva1269I GAATGC 1 cut(s) 577
MwoI GCNNNNNNNGC 1 cut(s) 266
NdeII GATC 1 cut(s) 534
NlaIII CATG 8 cut(s) 65, 88, 112, 142, 207, 445, 462, 562
NlaIV GGNNCC 1 cut(s) 82
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 1 cut(s) 207
PctI GAATGC 1 cut(s) 577
PfeI GAWTC 1 cut(s) 121
PkrI GCNGC 2 cut(s) 468, 635
PleI GAGTC 1 cut(s) 533
PpsI GAGTC 1 cut(s) 533
PshBI ATTAAT 1 cut(s) 68
PsiI TTATAA 1 cut(s) 326
PspN4I GGNNCC 1 cut(s) 82
RsaI GTAC 3 cut(s) 99, 178, 208
RsaNI GTAC 3 cut(s) 98, 177, 207
SaqAI TTAA 3 cut(s) 68, 522, 572
SatI GCNGC 2 cut(s) 467, 634
Sau3AI GATC 1 cut(s) 534
SchI GAGTC 1 cut(s) 533
SetI ASST 4 cut(s) 345, 471, 624, 630
SfaNI GCATC 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 3 cut(s) 14, 213, 307
SspMI CTAG 2 cut(s) 74, 165
StyI CCWWGG 2 cut(s) 144, 164
TaaI ACNGT 2 cut(s) 244, 338
TaqI TCGA 1 cut(s) 537
TasI AATT 3 cut(s) 14, 213, 307
TatI WGTACW 1 cut(s) 206
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 3 cut(s) 68, 522, 572
Tru9I TTAA 3 cut(s) 68, 522, 572
TseI GCWGC 2 cut(s) 466, 633
TspDTI ATGAA 5 cut(s) 97, 101, 339, 447, 458
Vha464I CTTAAG 1 cut(s) 521
VspI ATTAAT 1 cut(s) 68
XapI RAATTY 2 cut(s) 14, 213
XceI RCATGY 1 cut(s) 207
XmaJI CCTAGG 1 cut(s) 164
XspI CTAG 2 cut(s) 74, 165
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.