pycom04g03780

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000912
Physical Location & Seq
Reverse (-)
3380969 .. 3382107
1139 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g03780.2

Sequence Viewer

Length: 858 bp
ATGGCCTCCCGATTCATCGACGTCGCAGCCTCTTCTCCTTCCTCTTCGTTTTCTTCTAGGGTTCCGAACTGCAACCAACTCTCCTGCTTCTTCAGTTCTATGCCATCCAAATCCCCAAGGTACCTTCATCGTCATCCTTCCAACACTTCTTCTTTCTCACTCCCTCGGAGCCGCATAGACTGTTCTCTAAGATCCGCCATCGAAGATACCAATGGTACATGCCACGGCAAAGGCGAAATCGAGCATTCGATTTTGACGGCTTTGTCACCGTTGGACGGCCGTTATTGGGGTAAAGTCAAGGAATTGGCTCCTTTTATGAGCGAATATGGCCTTATCTACTACAGAGTTTTCGTTGAGATCAAATGGCTGTTGAAACTTTCACAAATCCCTGAGGTCACAGAGGTGCCCAGTTTCAGTGGAGATGCTCGATCTTACTTGCAAGGAATCATCGATGGATTTAGCGTCGATGATGCAGCGGAAATAAAGAAAATTGAGAAAATTACTAATCATGATGTGAAAGCAGTAGAGTATTTTTTGAAACAGAAATGCGGTTTGCATCCAGAGATAGCTAAGGTGCTCGAGTTTTTCCATTTTGCTTGCACATCTGAGGACATCAACAATCTTGCTCATGCGTTGATGTTAAAAGATGCTATCAACAATGTTATGTTTCCTGTCATGGATGAGCTGGTTGAGGCAATATGTGACATGTCAAAGGATAATGCTTCCGTTCCCATGCTTTCTCGCACGCACGGTCAGACTGCGTCACCCACCACTTTGGGGAAGGAAATGGCAATTTTCGCTGTCAGGTTAAGCATACAAAGTAGGAAATTACAATGCTCATCTTGTTGCATATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

286

Amino Acids

31.69

Weight (kDa)

6.75

Isoelectric Point (pI)

55.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 262
AatII GACGTC 1 cut(s) 24
Acc65I GGTACC 1 cut(s) 120
AccB1I GGYRCC 2 cut(s) 120, 403
AciI CCGC 4 cut(s) 172, 195, 476, 549
AclWI GGATC 1 cut(s) 186
AcoI YGGCCR 1 cut(s) 277
AcuI CTGAAG 1 cut(s) 76
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 2 cut(s) 122, 217
AfiI CCNNNNNNNGG 3 cut(s) 275, 286, 777
AflIII ACRYGT 1 cut(s) 705
AgsI TTSAA 2 cut(s) 373, 538
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 2 cut(s) 569, 685
AluI AGCT 2 cut(s) 569, 685
Alw21I GWGCWC 1 cut(s) 579
AlwI GGATC 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 3 cut(s) 3, 277, 328
ApeKI GCWGC 2 cut(s) 26, 473
Asp718I GGTACC 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 258, 756
AvaI CYCGRG 1 cut(s) 578
AxyI CCTNAGG 1 cut(s) 390
BaeGI GKGCMC 1 cut(s) 408
BanI GGYRCC 2 cut(s) 120, 403
Bbv12I GWGCWC 1 cut(s) 579
BbvI GCAGC 2 cut(s) 38, 485
BccI CCATC 3 cut(s) 112, 206, 446
BceAI ACGGC 4 cut(s) 241, 264, 273, 292
BfaI CTAG 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 340
BisI GCNGC 3 cut(s) 27, 172, 474
BlsI GCNGC 3 cut(s) 28, 173, 475
BmeT110I CYCGRG 1 cut(s) 578
BmiI GGNNCC 5 cut(s) 63, 122, 170, 309, 405
BmrI ACTGGG 1 cut(s) 402
BmsI GCATC 4 cut(s) 412, 460, 565, 637
BmuI ACTGGG 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 570
Bsa29I ATCGAT 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 3 cut(s) 116, 164, 223
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 3 cut(s) 275, 286, 777
Bse1I ACTGG 1 cut(s) 408
Bse21I CCTNAGG 1 cut(s) 390
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 3 cut(s) 116, 164, 223
BseGI GGATG 4 cut(s) 104, 133, 556, 685
BseLI CCNNNNNNNGG 3 cut(s) 275, 286, 777
BseMII CTCAG 2 cut(s) 381, 597
BseNI ACTGG 1 cut(s) 408
BseSI GKGCMC 1 cut(s) 408
BseX3I CGGCCG 1 cut(s) 277
BseXI GCAGC 2 cut(s) 38, 485
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 3 cut(s) 5, 279, 330
BshNI GGYRCC 2 cut(s) 120, 403
BshVI ATCGAT 1 cut(s) 450
BsiEI CGRYCG 1 cut(s) 280
BsiHKAI GWGCWC 1 cut(s) 579
BsiHKCI CYCGRG 1 cut(s) 578
BslI CCNNNNNNNGG 3 cut(s) 275, 286, 777
BsmI GAATGC 1 cut(s) 244
BsnI GGCC 3 cut(s) 5, 279, 330
BsoBI CYCGRG 1 cut(s) 578
Bsp1286I GDGCHC 2 cut(s) 408, 579
Bsp143I GATC 3 cut(s) 191, 357, 428
BspACI CCGC 4 cut(s) 172, 195, 476, 549
BspANI GGCC 3 cut(s) 5, 279, 330
BspCNI CTCAG 2 cut(s) 382, 598
BspDI ATCGAT 1 cut(s) 450
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 5 cut(s) 63, 122, 170, 309, 405
BspPI GGATC 1 cut(s) 186
BspT107I GGYRCC 2 cut(s) 120, 403
BsrI ACTGG 1 cut(s) 408
BssECI CCNNGG 3 cut(s) 116, 164, 223
BssMI GATC 3 cut(s) 191, 357, 428
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 116
Bst4CI ACNGT 3 cut(s) 182, 270, 752
Bst6I CTCTTC 2 cut(s) 37, 49
BstACI GRCGYC 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 598, 746
BstDEI CTNAG 4 cut(s) 188, 390, 570, 606
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 4 cut(s) 104, 133, 556, 685
BstKTI GATC 3 cut(s) 194, 360, 431
BstMBI GATC 3 cut(s) 191, 357, 428
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 2 cut(s) 327, 797
BstNSI RCATGY 2 cut(s) 222, 709
BstSFI CTRYAG 1 cut(s) 340
BstSLI GKGCMC 1 cut(s) 408
BstV1I GCAGC 2 cut(s) 38, 485
BstX2I RGATCY 1 cut(s) 191
BstXI CCANNNNNNTGG 1 cut(s) 775
BstYI RGATCY 1 cut(s) 191
BstZI CGGCCG 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 450
Bsu36I CCTNAGG 1 cut(s) 390
BsuRI GGCC 3 cut(s) 5, 279, 330
BsuTUI ATCGAT 1 cut(s) 450
BtgI CCRYGG 1 cut(s) 223
BtsCI GGATG 4 cut(s) 104, 133, 556, 685
BtsIMutI CAGTG 1 cut(s) 421
Cac8I GCNNGC 2 cut(s) 598, 746
CciI TCATGA 1 cut(s) 508
ClaI ATCGAT 1 cut(s) 450
CseI GACGC 2 cut(s) 451, 750
Csp6I GTAC 2 cut(s) 121, 216
CviAII CATG 6 cut(s) 219, 509, 629, 676, 706, 733
CviQI GTAC 2 cut(s) 121, 216
DdeI CTNAG 4 cut(s) 188, 390, 570, 606
DpnI GATC 3 cut(s) 193, 359, 430
DpnII GATC 3 cut(s) 191, 357, 428
DrdI GACNNNNNNGTC 1 cut(s) 262
DseDI GACNNNNNNGTC 1 cut(s) 262
EaeI YGGCCR 1 cut(s) 277
EagI CGGCCG 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 37, 49
EarI CTCTTC 2 cut(s) 37, 49
EciI GGCGGA 1 cut(s) 184
EclXI CGGCCG 1 cut(s) 277
Eco130I CCWWGG 1 cut(s) 116
Eco52I CGGCCG 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 76
Eco81I CCTNAGG 1 cut(s) 390
Eco88I CYCGRG 1 cut(s) 578
EcoT14I CCWWGG 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 116
FaeI CATG 6 cut(s) 222, 512, 632, 679, 709, 736
FatI CATG 6 cut(s) 218, 508, 628, 675, 705, 732
Fnu4HI GCNGC 3 cut(s) 27, 172, 474
FokI GGATG 4 cut(s) 91, 120, 543, 692
Fsp4HI GCNGC 3 cut(s) 27, 172, 474
FspBI CTAG 1 cut(s) 57
GluI GCNGC 3 cut(s) 27, 172, 474
HaeIII GGCC 3 cut(s) 5, 279, 330
HgaI GACGC 2 cut(s) 451, 750
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 6 cut(s) 222, 512, 632, 679, 709, 736
HinfI GANTC 2 cut(s) 12, 444
HphI GGTGA 2 cut(s) 258, 756
Hpy188I TCNGA 4 cut(s) 66, 168, 607, 756
Hpy188III TCNNGA 4 cut(s) 9, 509, 560, 855
Hpy99I CGWCG 3 cut(s) 23, 26, 467
HpyAV CCTTC 4 cut(s) 48, 134, 147, 775
HpyCH4III ACNGT 3 cut(s) 182, 270, 752
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 6 cut(s) 72, 439, 473, 556, 600, 849
HpyF10VI GCNNNNNNNGC 2 cut(s) 327, 797
HpyF3I CTNAG 4 cut(s) 188, 390, 570, 606
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 6 cut(s) 222, 512, 632, 679, 709, 736
KpnI GGTACC 1 cut(s) 124
Kzo9I GATC 3 cut(s) 191, 357, 428
LmnI GCTCC 2 cut(s) 168, 313
LpnPI CCDG 7 cut(s) 97, 402, 421, 573, 671, 684, 790
Lsp1109I GCAGC 2 cut(s) 38, 485
LweI GCATC 4 cut(s) 412, 460, 565, 637
MaeI CTAG 1 cut(s) 57
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 4 cut(s) 264, 394, 701, 762
MalI GATC 3 cut(s) 193, 359, 430
MboI GATC 3 cut(s) 191, 357, 428
MboII GAAGA 6 cut(s) 24, 36, 45, 82, 141, 215
MflI RGATCY 1 cut(s) 191
MhlI GDGCHC 2 cut(s) 408, 579
MluCI AATT 5 cut(s) 302, 489, 498, 792, 827
MmeI TCCRAC 2 cut(s) 165, 252
MnlI CCTC 8 cut(s) 16, 40, 52, 174, 385, 394, 601, 685
MseI TTAA 2 cut(s) 641, 809
MslI CAYNNNNRTG 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 476
Mva1269I GAATGC 1 cut(s) 244
MwoI GCNNNNNNNGC 2 cut(s) 327, 797
NdeII GATC 3 cut(s) 191, 357, 428
NlaIII CATG 6 cut(s) 222, 512, 632, 679, 709, 736
NlaIV GGNNCC 5 cut(s) 63, 122, 170, 309, 405
NmuCI GTSAC 4 cut(s) 264, 394, 701, 762
NspI RCATGY 2 cut(s) 222, 709
OliI CACNNNNGTG 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 578
PagI TCATGA 1 cut(s) 508
PciI ACATGT 1 cut(s) 705
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 244
PfeI GAWTC 2 cut(s) 12, 444
PflFI GACNNNGTC 1 cut(s) 760
PkrI GCNGC 3 cut(s) 28, 173, 475
PscI ACATGT 1 cut(s) 705
PspN4I GGNNCC 5 cut(s) 63, 122, 170, 309, 405
PspXI VCTCGAGB 1 cut(s) 578
PsuI RGATCY 1 cut(s) 191
PsyI GACNNNGTC 1 cut(s) 760
RsaI GTAC 2 cut(s) 122, 217
RsaNI GTAC 2 cut(s) 121, 216
RseI CAYNNNNRTG 1 cut(s) 401
SaqAI TTAA 2 cut(s) 641, 809
SatI GCNGC 3 cut(s) 27, 172, 474
Sau3AI GATC 3 cut(s) 191, 357, 428
SduI GDGCHC 2 cut(s) 408, 579
SetI ASST 9 cut(s) 24, 122, 126, 396, 405, 571, 576, 687, 809
SfaNI GCATC 4 cut(s) 412, 460, 565, 637
SfcI CTRYAG 1 cut(s) 340
Sfr274I CTCGAG 1 cut(s) 578
SlaI CTCGAG 1 cut(s) 578
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
Sse9I AATT 5 cut(s) 302, 489, 498, 792, 827
SsiI CCGC 4 cut(s) 172, 195, 476, 549
SspMI CTAG 1 cut(s) 57
StyI CCWWGG 1 cut(s) 116
TaaI ACNGT 3 cut(s) 182, 270, 752
TaiI ACGT 1 cut(s) 24
TaqI TCGA 8 cut(s) 18, 201, 240, 248, 427, 450, 465, 579
TasI AATT 5 cut(s) 302, 489, 498, 792, 827
TauI GCSGC 1 cut(s) 174
TfiI GAWTC 2 cut(s) 12, 444
Tru1I TTAA 2 cut(s) 641, 809
Tru9I TTAA 2 cut(s) 641, 809
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 4 cut(s) 264, 394, 701, 762
TseI GCWGC 2 cut(s) 26, 473
Tsp45I GTSAC 4 cut(s) 264, 394, 701, 762
TspDTI ATGAA 2 cut(s) 4, 116
TspGWI ACGGA 1 cut(s) 715
TspRI CASTG 1 cut(s) 421
Tth111I GACNNNGTC 1 cut(s) 760
XceI RCATGY 2 cut(s) 222, 709
XhoI CTCGAG 1 cut(s) 578
XspI CTAG 1 cut(s) 57
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.