Rorug07G0013500

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000912
Physical Location & Seq
Forward (+)
1043034 .. 1057106
14073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0013500.1

Sequence Viewer

Length: 1779 bp
ATGGGGGCTTCTCAGGCCGCCAGTCTATGGGTTCTGCTGGGCTTGGGCTTGGCCGGAATCCTGATAATGACTCGGAAGCTGAAGCAGGTCATCCGAGAAGACTTCGGCGCCTTCGTTGAGAAGCTTCAGCTCCTCCCTCCTCCGCAGCCGGCTCCGCCCAAAGCTCCCCACCCCCTCACCGGCCTCACCTTCGCCGTTTCCGACGTGTTTGATATAGAGGGATATGTGACTGGATTCGGTCATCCTGATTGGGCTAAAACACATGATCCAGCTTCTCGGACATCTCCTGTGGTTACCATGCTTGTTGAAGGAGGAGCCACATGCATTGGAAAAACTGTTGTGGATGAATTGGCATATAGCATTTCTGGAGAAAATGAGCATTACTTTACACCTACCAACCCTACAGCGCCATCAAAAACACCAGGTGGATCCTCTAGTGGAGCTGCGGTGGCTGTAGCGGCTAATCTTGCTGACTTTTCCCTGGGTATTGATACTGTTGGTGGTGTTAGAGTACCTGCGGCGTTTTGTGGCATTATGGGATTCCGACCTTCACATGGGGCAGTTTCTCACACTGGAATTATACCTGTTTCTACAAGTCTTGATACCGTTGGATGGTTTGCCAAGGATCCTAAGGTTCTAAGTAGGGTTGGTCACGTGTTGCTGCAACTTCCCCTTCCATTTTCACGCAGTCCAAGACAAATTGTGATGGCTGATGATTGCCTACAACAAGTAAAGGTTCCTACTGACAGGATTGCTCAAGTAGTTGTCAAATCCACTGAGAAGCTTTTTGGAAGACAAGTATTGAAGCATGAAAATCTTGAGGACTATTTCAATTTGAAAGTTCCAAGCTTGAGAAAGTTTCATGGTGAGAAGACAAATGGTGTTGTAAAGGCTTCATCAATAAAACTGCTTGCCAATGTTGCTCAGTTTCTTCAAAGATATGAGTTCAAACAAAATCATGACGAATGGATTAATTTGGTGAAGCCTACTTTGGATCCTGCTACTTCAGCTCAGATATGTGGGCCACCGGAGTCATCAGAAATGGAGGTTGAGATCTGCAAGTCCATTAAAAATGAATTGCATGCAGCTATCAACTCTCTATTGAAGGATGATGGGATTCTGGTGATCCCGACTGTGGCAGATCCTCCTTCAAAACTTGGTGGGAAGACTCTCCTCTCTGAGGAATTCCGTAGTCGTGCTTTTGGTTTGTTAAGTATTGCAAGCCTATCAGGATGCTGTCAGGTCACAATACCACTTGGACTTCATAACAAGAATCCTGTTTCAGTTTCTTTTATAGCCAGGCATGGGGGTGATCGCTTTTTACTAGATACATTGCAGACAATGTACAAATCTTTACAGGAGCAGGCTGATCTTGTTACAAACACCAAATTATCAAAAAATGCACTCACCAGAGAGCAATCTGCTGAAGTTTCCAAGGAAAAGGGAAACAAAGCATACAAAGACAAAGAGTATCAGAAAGCTATTGGGTTTTATTCAGAAGCCATAAAACTTGGTGGCAATAATGCAACATATTACAGTAACAGGGCCGCAGCATATCTGGAAATTGGAAGTTTCGTTCAAGCTGAGGCGGATTGTAGTCAGGCTATCAACCTCGACAAAAAGAATGTGAAGGCTTATTTTCGAAGAGGCACTGCAAGGGAGGTGCTTGGGTACTATAAGGAGGCAATTGAAGACTTTAAATATGCACATGTTCTTGAACCGACGAATAAAAGAGCAGCCGCTGCTGCTGATAGATTGAGGAAGTTATTTCTTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

592

Amino Acids

64.29

Weight (kDa)

8.83

Isoelectric Point (pI)

33.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 56 - 214 1.3e-44 Amidase
TPR_BSK1_C PF25575 446 - 542 1.4e-07 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 76, 523
AccB1I GGYRCC 1 cut(s) 107
AccB7I CCANNNNNTGG 1 cut(s) 27
AciI CCGC 9 cut(s) 18, 143, 155, 446, 458, 518, 1548, 1589, 1740
AclWI GGATC 9 cut(s) 260, 423, 436, 620, 633, 989, 1002, 1120, 1136
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 1 cut(s) 1184
AcuI CTGAAG 4 cut(s) 101, 110, 990, 1446
AcvI CACGTG 1 cut(s) 655
AcyI GRCGYC 1 cut(s) 108
AdeI CACNNNGTG 1 cut(s) 425
AfaI GTAC 3 cut(s) 513, 1346, 1673
AfiI CCNNNNNNNGG 7 cut(s) 27, 179, 554, 612, 1135, 1180, 1305
AflIII ACRYGT 3 cut(s) 204, 654, 1708
AjiI CACGTC 1 cut(s) 205
AjnI CCWGG 3 cut(s) 421, 480, 1298
AjuI GAANNNNNNNTTGG 2 cut(s) 974, 1006
AloI GAACNNNNNNTCC 2 cut(s) 1560, 1592
AlwI GGATC 9 cut(s) 260, 423, 436, 620, 633, 989, 1002, 1120, 1136
AlwNI CAGNNNCTG 1 cut(s) 1742
AoxI GGCC 5 cut(s) 15, 51, 181, 1022, 1545
ApeKI GCWGC 8 cut(s) 145, 443, 661, 1085, 1550, 1736, 1742, 1745
ApoI RAATTY 1 cut(s) 1184
AseI ATTAAT 1 cut(s) 972
AspLEI GCGC 2 cut(s) 110, 409
AspS9I GGNCC 2 cut(s) 1022, 1545
AsuHPI GGTGA 7 cut(s) 169, 178, 878, 991, 1135, 1322, 1399
AsuII TTCGAA 1 cut(s) 1642
AxyI CCTNAGG 1 cut(s) 630
BamHI GGATCC 3 cut(s) 428, 625, 994
BanI GGYRCC 1 cut(s) 107
BbrPI CACGTG 1 cut(s) 655
BbsI GAAGAC 5 cut(s) 105, 799, 878, 1172, 1698
BbvCI CCTCAGC 1 cut(s) 1584
BbvI GCAGC 8 cut(s) 157, 430, 648, 1097, 1562, 1729, 1732, 1748
BccI CCATC 4 cut(s) 418, 606, 700, 1106
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 3 cut(s) 423, 482, 1300
BfaI CTAG 2 cut(s) 435, 1325
BfmI CTRYAG 2 cut(s) 402, 453
BfoI RGCGCY 2 cut(s) 111, 410
BfuAI ACCTGC 2 cut(s) 76, 523
BglII AGATCT 1 cut(s) 1053
Bme1390I CCNGG 3 cut(s) 423, 482, 1300
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 2 cut(s) 1022, 1545
BmiI GGNNCC 7 cut(s) 109, 153, 316, 430, 627, 738, 996
BmrFI CCNGG 3 cut(s) 423, 482, 1300
BmsI GCATC 1 cut(s) 1223
BpiI GAAGAC 5 cut(s) 105, 799, 878, 1172, 1698
BpmI CTGGAG 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 1584
Bpu14I TTCGAA 1 cut(s) 1642
BpuEI CTTGAG 3 cut(s) 741, 839, 871
BsaAI YACGTR 1 cut(s) 655
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 4 cut(s) 480, 481, 621, 1434
BsaWI WCCGGW 1 cut(s) 1027
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 7 cut(s) 27, 179, 554, 612, 1135, 1180, 1305
Bse118I RCCGGY 2 cut(s) 148, 179
Bse1I ACTGG 3 cut(s) 21, 235, 577
Bse21I CCTNAGG 1 cut(s) 630
Bse3DI GCAATG 1 cut(s) 1331
BseBI CCWGG 3 cut(s) 423, 482, 1300
BseDI CCNNGG 4 cut(s) 480, 481, 621, 1434
BseGI GGATG 6 cut(s) 90, 241, 349, 617, 1114, 1238
BseLI CCNNNNNNNGG 7 cut(s) 27, 179, 554, 612, 1135, 1180, 1305
BseMI GCAATG 1 cut(s) 1331
BseMII CTCAG 6 cut(s) 26, 768, 938, 1025, 1170, 1575
BseNI ACTGG 3 cut(s) 21, 235, 577
BseRI GAGGAG 4 cut(s) 122, 129, 327, 1163
BseXI GCAGC 8 cut(s) 157, 430, 648, 1097, 1562, 1729, 1732, 1748
BseYI CCCAGC 1 cut(s) 37
BshFI GGCC 5 cut(s) 17, 53, 183, 1024, 1547
BshNI GGYRCC 1 cut(s) 107
BsiSI CCGG 4 cut(s) 54, 149, 180, 1028
BslI CCNNNNNNNGG 7 cut(s) 27, 179, 554, 612, 1135, 1180, 1305
BsnI GGCC 5 cut(s) 17, 53, 183, 1024, 1547
Bsp119I TTCGAA 1 cut(s) 1642
Bsp1407I TGTACA 1 cut(s) 1344
Bsp143I GATC 9 cut(s) 265, 428, 625, 994, 1053, 1125, 1141, 1312, 1369
BspACI CCGC 9 cut(s) 18, 143, 155, 446, 458, 518, 1548, 1589, 1740
BspANI GGCC 5 cut(s) 17, 53, 183, 1024, 1547
BspCNI CTCAG 6 cut(s) 25, 769, 937, 1024, 1171, 1576
BspHI TCATGA 1 cut(s) 958
BspLI GGNNCC 7 cut(s) 109, 153, 316, 430, 627, 738, 996
BspMI ACCTGC 2 cut(s) 76, 523
BspPI GGATC 9 cut(s) 260, 423, 436, 620, 633, 989, 1002, 1120, 1136
BspT104I TTCGAA 1 cut(s) 1642
BspT107I GGYRCC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 1331
BsrFI RCCGGY 2 cut(s) 148, 179
BsrGI TGTACA 1 cut(s) 1344
BsrI ACTGG 3 cut(s) 21, 235, 577
BssAI RCCGGY 2 cut(s) 148, 179
BssECI CCNNGG 4 cut(s) 480, 481, 621, 1434
BssMI GATC 9 cut(s) 265, 428, 625, 994, 1053, 1125, 1141, 1312, 1369
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 2 cut(s) 621, 1434
Bst2UI CCWGG 3 cut(s) 423, 482, 1300
Bst4CI ACNGT 5 cut(s) 337, 496, 607, 1135, 1538
Bst6I CTCTTC 1 cut(s) 1639
BstACI GRCGYC 1 cut(s) 108
BstAPI GCANNNNNTGC 1 cut(s) 1742
BstAUI TGTACA 1 cut(s) 1344
BstBAI YACGTR 1 cut(s) 655
BstBI TTCGAA 1 cut(s) 1642
BstC8I GCNNGC 5 cut(s) 150, 912, 1083, 1222, 1365
BstDEI CTNAG 8 cut(s) 12, 630, 638, 777, 924, 1011, 1179, 1584
BstEII GGTNACC 1 cut(s) 292
BstENI CCTNNNNNAGG 1 cut(s) 1178
BstF5I GGATG 6 cut(s) 90, 241, 349, 617, 1114, 1238
BstH2I RGCGCY 2 cut(s) 111, 410
BstHHI GCGC 2 cut(s) 110, 409
BstKTI GATC 9 cut(s) 268, 431, 628, 997, 1056, 1128, 1144, 1315, 1372
BstMBI GATC 9 cut(s) 265, 428, 625, 994, 1053, 1125, 1141, 1312, 1369
BstMWI GCNNNNNNNGC 9 cut(s) 14, 154, 449, 458, 467, 920, 1007, 1742, 1745
BstNI CCWGG 3 cut(s) 423, 482, 1300
BstNSI RCATGY 3 cut(s) 324, 1085, 1712
BstPI GGTNACC 1 cut(s) 292
BstSCI CCNGG 3 cut(s) 421, 480, 1298
BstSFI CTRYAG 2 cut(s) 402, 453
BstV1I GCAGC 8 cut(s) 157, 430, 648, 1097, 1562, 1729, 1732, 1748
BstV2I GAAGAC 5 cut(s) 105, 799, 878, 1172, 1698
BstX2I RGATCY 5 cut(s) 428, 625, 994, 1053, 1141
BstYI RGATCY 5 cut(s) 428, 625, 994, 1053, 1141
Bsu36I CCTNAGG 1 cut(s) 630
BsuRI GGCC 5 cut(s) 17, 53, 183, 1024, 1547
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 6 cut(s) 90, 241, 349, 617, 1114, 1238
BtsI GCAGTG 1 cut(s) 1650
BtsIMutI CAGTG 3 cut(s) 570, 774, 1650
BveI ACCTGC 2 cut(s) 76, 523
Cac8I GCNNGC 5 cut(s) 150, 912, 1083, 1222, 1365
CaiI CAGNNNCTG 1 cut(s) 1742
CciI TCATGA 1 cut(s) 958
CfoI GCGC 2 cut(s) 110, 409
Cfr10I RCCGGY 2 cut(s) 148, 179
Cfr13I GGNCC 2 cut(s) 1022, 1545
CsiI ACCWGGT 1 cut(s) 421
Csp6I GTAC 3 cut(s) 512, 1345, 1672
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CviQI GTAC 3 cut(s) 512, 1345, 1672
DdeI CTNAG 8 cut(s) 12, 630, 638, 777, 924, 1011, 1179, 1584
DinI GGCGCC 1 cut(s) 109
DpnI GATC 9 cut(s) 267, 430, 627, 996, 1055, 1127, 1143, 1314, 1371
DpnII GATC 9 cut(s) 265, 428, 625, 994, 1053, 1125, 1141, 1312, 1369
DraI TTTAAA 1 cut(s) 1699
DraIII CACNNNGTG 1 cut(s) 425
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 1639
EarI CTCTTC 1 cut(s) 1639
EciI GGCGGA 2 cut(s) 144, 1604
Eco130I CCWWGG 2 cut(s) 621, 1434
Eco57I CTGAAG 4 cut(s) 101, 110, 990, 1446
Eco72I CACGTG 1 cut(s) 655
Eco81I CCTNAGG 1 cut(s) 630
Eco91I GGTNACC 1 cut(s) 292
EcoNI CCTNNNNNAGG 1 cut(s) 1178
EcoO65I GGTNACC 1 cut(s) 292
EcoRI GAATTC 1 cut(s) 1184
EcoRII CCWGG 3 cut(s) 421, 480, 1298
EcoT14I CCWWGG 2 cut(s) 621, 1434
EcoT22I ATGCAT 1 cut(s) 326
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
ErhI CCWWGG 2 cut(s) 621, 1434
FokI GGATG 6 cut(s) 77, 228, 356, 624, 1121, 1245
FspBI CTAG 2 cut(s) 435, 1325
GlaI GCGC 2 cut(s) 109, 408
GsaI CCCAGC 1 cut(s) 41
GsuI CTGGAG 1 cut(s) 387
HaeII RGCGCY 2 cut(s) 111, 410
HaeIII GGCC 5 cut(s) 17, 53, 183, 1024, 1547
HapII CCGG 4 cut(s) 54, 149, 180, 1028
HhaI GCGC 2 cut(s) 110, 409
Hin1I GRCGYC 1 cut(s) 108
Hin6I GCGC 2 cut(s) 108, 407
HinP1I GCGC 2 cut(s) 108, 407
HindIII AAGCTT 3 cut(s) 122, 782, 847
HinfI GANTC 8 cut(s) 57, 70, 234, 540, 1031, 1117, 1168, 1273
HpaII CCGG 4 cut(s) 54, 149, 180, 1028
HphI GGTGA 7 cut(s) 169, 178, 878, 991, 1135, 1322, 1399
Hpy99I CGWCG 2 cut(s) 206, 1726
HpyAV CCTTC 8 cut(s) 121, 199, 302, 558, 683, 1099, 1158, 1624
HpyCH4III ACNGT 5 cut(s) 337, 496, 607, 1135, 1538
HpyCH4IV ACGT 2 cut(s) 204, 654
HpyF10VI GCNNNNNNNGC 9 cut(s) 14, 154, 449, 458, 467, 920, 1007, 1742, 1745
HpyF3I CTNAG 8 cut(s) 12, 630, 638, 777, 924, 1011, 1179, 1584
HpySE526I ACGT 2 cut(s) 204, 654
Hsp92I GRCGYC 1 cut(s) 108
HspAI GCGC 2 cut(s) 108, 407
KasI GGCGCC 1 cut(s) 107
KroI GCCGGC 1 cut(s) 148
KroNI GCCGGC 1 cut(s) 150
Kzo9I GATC 9 cut(s) 265, 428, 625, 994, 1053, 1125, 1141, 1312, 1369
LmnI GCTCC 6 cut(s) 135, 157, 169, 314, 440, 1360
Lsp1109I GCAGC 8 cut(s) 157, 430, 648, 1097, 1562, 1729, 1732, 1748
LweI GCATC 1 cut(s) 1223
MabI ACCWGGT 1 cut(s) 421
MaeI CTAG 2 cut(s) 435, 1325
MaeII ACGT 2 cut(s) 204, 654
MaeIII GTNAC 6 cut(s) 226, 292, 650, 1243, 1375, 1538
MalI GATC 9 cut(s) 267, 430, 627, 996, 1055, 1127, 1143, 1314, 1371
MboI GATC 9 cut(s) 265, 428, 625, 994, 1053, 1125, 1141, 1312, 1369
MboII GAAGA 8 cut(s) 110, 804, 883, 923, 1177, 1656, 1703, 1763
MfeI CAATTG 1 cut(s) 1686
MflI RGATCY 5 cut(s) 428, 625, 994, 1053, 1141
Mly113I GGCGCC 1 cut(s) 108
MlyI GAGTC 3 cut(s) 64, 1040, 1162
MmeI TCCRAC 3 cut(s) 225, 568, 589
Mph1103I ATGCAT 1 cut(s) 326
MroNI GCCGGC 1 cut(s) 148
MseI TTAA 4 cut(s) 972, 1068, 1211, 1698
MslI CAYNNNNRTG 1 cut(s) 1308
MspA1I CMGCKG 1 cut(s) 1742
MspI CCGG 4 cut(s) 54, 149, 180, 1028
MspR9I CCNGG 3 cut(s) 423, 482, 1300
MunI CAATTG 1 cut(s) 1686
MvaI CCWGG 3 cut(s) 423, 482, 1300
MwoI GCNNNNNNNGC 9 cut(s) 14, 154, 449, 458, 467, 920, 1007, 1742, 1745
NaeI GCCGGC 1 cut(s) 150
NarI GGCGCC 1 cut(s) 108
NdeII GATC 9 cut(s) 265, 428, 625, 994, 1053, 1125, 1141, 1312, 1369
NgoMIV GCCGGC 1 cut(s) 148
NlaIV GGNNCC 7 cut(s) 109, 153, 316, 430, 627, 738, 996
NmuCI GTSAC 3 cut(s) 226, 650, 1243
NsiI ATGCAT 1 cut(s) 326
NspI RCATGY 3 cut(s) 324, 1085, 1712
NspV TTCGAA 1 cut(s) 1642
PaeI GCATGC 1 cut(s) 1085
PagI TCATGA 1 cut(s) 958
PasI CCCWGGG 1 cut(s) 481
PciI ACATGT 1 cut(s) 1708
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 198
PdiI GCCGGC 1 cut(s) 150
PfeI GAWTC 5 cut(s) 57, 234, 540, 1117, 1273
PflMI CCANNNNNTGG 1 cut(s) 27
PleI GAGTC 3 cut(s) 64, 1039, 1162
PluTI GGCGCC 1 cut(s) 111
PmaCI CACGTG 1 cut(s) 655
PmlI CACGTG 1 cut(s) 655
PpsI GAGTC 3 cut(s) 64, 1039, 1162
Ppu21I YACGTR 1 cut(s) 655
PscI ACATGT 1 cut(s) 1708
PshBI ATTAAT 1 cut(s) 972
Psp6I CCWGG 3 cut(s) 421, 480, 1298
PspCI CACGTG 1 cut(s) 655
PspEI GGTNACC 1 cut(s) 292
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 3 cut(s) 421, 480, 1298
PspN4I GGNNCC 7 cut(s) 109, 153, 316, 430, 627, 738, 996
PspPI GGNCC 2 cut(s) 1022, 1545
PstNI CAGNNNCTG 1 cut(s) 1742
PsuI RGATCY 5 cut(s) 428, 625, 994, 1053, 1141
RsaI GTAC 3 cut(s) 513, 1346, 1673
RsaNI GTAC 3 cut(s) 512, 1345, 1672
RseI CAYNNNNRTG 1 cut(s) 1308
SaqAI TTAA 4 cut(s) 972, 1068, 1211, 1698
Sau3AI GATC 9 cut(s) 265, 428, 625, 994, 1053, 1125, 1141, 1312, 1369
Sau96I GGNCC 2 cut(s) 1022, 1545
SchI GAGTC 3 cut(s) 64, 1040, 1162
ScrFI CCNGG 3 cut(s) 423, 482, 1300
SexAI ACCWGGT 1 cut(s) 421
SfaNI GCATC 1 cut(s) 1223
SfcI CTRYAG 2 cut(s) 402, 453
SfoI GGCGCC 1 cut(s) 109
SfuI TTCGAA 1 cut(s) 1642
SmiMI CAYNNNNRTG 1 cut(s) 1308
SmlI CTYRAG 3 cut(s) 756, 818, 850
SmoI CTYRAG 3 cut(s) 756, 818, 850
SphI GCATGC 1 cut(s) 1085
SsiI CCGC 9 cut(s) 18, 143, 155, 446, 458, 518, 1548, 1589, 1740
SspDI GGCGCC 1 cut(s) 107
SspMI CTAG 2 cut(s) 435, 1325
StyD4I CCNGG 3 cut(s) 421, 480, 1298
StyI CCWWGG 2 cut(s) 621, 1434
TaaI ACNGT 5 cut(s) 337, 496, 607, 1135, 1538
TaiI ACGT 2 cut(s) 207, 657
TaqI TCGA 2 cut(s) 1614, 1642
TaqII GACCGA 1 cut(s) 227
TatI WGTACW 1 cut(s) 1344
TauI GCSGC 5 cut(s) 20, 461, 521, 1550, 1742
TfiI GAWTC 5 cut(s) 57, 234, 540, 1117, 1273
Tru1I TTAA 4 cut(s) 972, 1068, 1211, 1698
Tru9I TTAA 4 cut(s) 972, 1068, 1211, 1698
TscAI CASTG 3 cut(s) 577, 781, 1657
TseFI GTSAC 3 cut(s) 226, 650, 1243
TseI GCWGC 8 cut(s) 145, 443, 661, 1085, 1550, 1736, 1742, 1745
Tsp45I GTSAC 3 cut(s) 226, 650, 1243
TspDTI ATGAA 6 cut(s) 360, 825, 851, 885, 1089, 1253
TspGWI ACGGA 1 cut(s) 1178
TspRI CASTG 3 cut(s) 577, 781, 1657
Van91I CCANNNNNTGG 1 cut(s) 27
VspI ATTAAT 1 cut(s) 972
XagI CCTNNNNNAGG 1 cut(s) 1178
XapI RAATTY 1 cut(s) 1184
XceI RCATGY 3 cut(s) 324, 1085, 1712
XspI CTAG 2 cut(s) 435, 1325
Zsp2I ATGCAT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.