Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 259 |
| AccB1I | GGYRCC | 1 cut(s) | 400 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1721 |
| AccIII | TCCGGA | 1 cut(s) | 244 |
| AciI | CCGC | 8 cut(s) | 73, 201, 204, 207, 518, 1063, 1318, 1967 |
| AclWI | GGATC | 1 cut(s) | 1579 |
| AcoI | YGGCCR | 4 cut(s) | 274, 1285, 1605, 1723 |
| AcsI | RAATTY | 3 cut(s) | 406, 1830, 2027 |
| AcuI | CTGAAG | 7 cut(s) | 389, 408, 945, 1319, 1469, 1542, 1857 |
| AfaI | GTAC | 8 cut(s) | 14, 527, 742, 1067, 1455, 1730, 2149, 2165 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 272, 1721, 1732, 1860, 2123 |
| AjiI | CACGTC | 1 cut(s) | 600 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1158, 1190 |
| Alw21I | GWGCWC | 2 cut(s) | 8, 1873 |
| Alw26I | GTCTC | 3 cut(s) | 68, 267, 974 |
| AlwI | GGATC | 1 cut(s) | 1579 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 647, 1478 |
| Ama87I | CYCGRG | 1 cut(s) | 1685 |
| Aor13HI | TCCGGA | 1 cut(s) | 244 |
| AoxI | GGCC | 8 cut(s) | 198, 274, 325, 756, 911, 1285, 1605, 1723 |
| ApeKI | GCWGC | 3 cut(s) | 719, 1234, 1315 |
| ApoI | RAATTY | 3 cut(s) | 406, 1830, 2027 |
| AseI | ATTAAT | 1 cut(s) | 462 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 223, 547, 2031 |
| AspLEI | GCGC | 1 cut(s) | 191 |
| AspS9I | GGNCC | 2 cut(s) | 198, 912 |
| AsuHPI | GGTGA | 5 cut(s) | 753, 1118, 1663, 1704, 2138 |
| AvaI | CYCGRG | 1 cut(s) | 1685 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1720, 1753 |
| BanI | GGYRCC | 1 cut(s) | 400 |
| BanII | GRGCYC | 1 cut(s) | 8 |
| BbsI | GAAGAC | 3 cut(s) | 500, 1110, 1598 |
| Bbv12I | GWGCWC | 2 cut(s) | 8, 1873 |
| BbvI | GCAGC | 3 cut(s) | 706, 1246, 1302 |
| BccI | CCATC | 5 cut(s) | 32, 443, 1651, 1715, 1892 |
| BceAI | ACGGC | 4 cut(s) | 270, 289, 771, 1592 |
| BciVI | GTATCC | 1 cut(s) | 1073 |
| BcoDI | GTCTC | 3 cut(s) | 68, 267, 974 |
| BfaI | CTAG | 2 cut(s) | 1185, 1922 |
| BfmI | CTRYAG | 3 cut(s) | 864, 1303, 1479 |
| BfuI | GTATCC | 1 cut(s) | 1073 |
| BisI | GCNGC | 6 cut(s) | 201, 204, 207, 720, 1235, 1316 |
| BlsI | GCNGC | 6 cut(s) | 202, 205, 208, 721, 1236, 1317 |
| BmcAI | AGTACT | 2 cut(s) | 527, 2165 |
| BmeT110I | CYCGRG | 1 cut(s) | 1685 |
| BmgBI | CACGTC | 1 cut(s) | 600 |
| BmgT120I | GGNCC | 2 cut(s) | 198, 912 |
| BmiI | GGNNCC | 3 cut(s) | 306, 402, 1755 |
| BmrI | ACTGGG | 1 cut(s) | 1741 |
| BmsI | GCATC | 5 cut(s) | 250, 457, 1917, 1982, 2073 |
| BmuI | ACTGGG | 1 cut(s) | 1741 |
| BpiI | GAAGAC | 3 cut(s) | 500, 1110, 1598 |
| Bpu10I | CCTNAGC | 2 cut(s) | 567, 2157 |
| BpuEI | CTTGAG | 2 cut(s) | 596, 816 |
| BsaAI | YACGTR | 1 cut(s) | 740 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1368, 1996 |
| BsaI | GGTCTC | 1 cut(s) | 68 |
| BsaJI | CCNNGG | 2 cut(s) | 2052, 2151 |
| BsaWI | WCCGGW | 1 cut(s) | 244 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 272, 1721, 1732, 1860, 2123 |
| Bse118I | RCCGGY | 2 cut(s) | 276, 1725 |
| Bse1I | ACTGG | 1 cut(s) | 1736 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1368, 1996 |
| BseAI | TCCGGA | 1 cut(s) | 244 |
| BseDI | CCNNGG | 2 cut(s) | 2052, 2151 |
| BseGI | GGATG | 6 cut(s) | 24, 148, 241, 553, 1726, 2048 |
| BseJI | GATNNNNATC | 2 cut(s) | 1368, 1996 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 272, 1721, 1732, 1860, 2123 |
| BseMII | CTCAG | 8 cut(s) | 193, 208, 437, 594, 1196, 1253, 1635, 1886 |
| BseNI | ACTGG | 1 cut(s) | 1736 |
| BseRI | GAGGAG | 1 cut(s) | 81 |
| BseX3I | CGGCCG | 1 cut(s) | 274 |
| BseXI | GCAGC | 3 cut(s) | 706, 1246, 1302 |
| Bsh1285I | CGRYCG | 1 cut(s) | 277 |
| BshFI | GGCC | 8 cut(s) | 200, 276, 327, 758, 913, 1287, 1607, 1725 |
| BshNI | GGYRCC | 1 cut(s) | 400 |
| BsiEI | CGRYCG | 1 cut(s) | 277 |
| BsiHKAI | GWGCWC | 2 cut(s) | 8, 1873 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1685 |
| BsiSI | CCGG | 4 cut(s) | 228, 245, 277, 1726 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 272, 1721, 1732, 1860, 2123 |
| BsmAI | GTCTC | 3 cut(s) | 68, 267, 974 |
| BsnI | GGCC | 8 cut(s) | 200, 276, 327, 758, 913, 1287, 1607, 1725 |
| Bso31I | GGTCTC | 1 cut(s) | 68 |
| BsoBI | CYCGRG | 1 cut(s) | 1685 |
| Bsp1286I | GDGCHC | 2 cut(s) | 8, 1873 |
| Bsp13I | TCCGGA | 1 cut(s) | 244 |
| Bsp143I | GATC | 4 cut(s) | 975, 1038, 1571, 1803 |
| Bsp19I | CCATGG | 1 cut(s) | 2151 |
| BspACI | CCGC | 8 cut(s) | 73, 201, 204, 207, 518, 1063, 1318, 1967 |
| BspANI | GGCC | 8 cut(s) | 200, 276, 327, 758, 913, 1287, 1607, 1725 |
| BspCNI | CTCAG | 8 cut(s) | 192, 207, 436, 595, 1197, 1254, 1636, 1885 |
| BspEI | TCCGGA | 1 cut(s) | 244 |
| BspHI | TCATGA | 1 cut(s) | 51 |
| BspLI | GGNNCC | 3 cut(s) | 306, 402, 1755 |
| BspMAI | CTGCAG | 1 cut(s) | 1483 |
| BspPI | GGATC | 1 cut(s) | 1579 |
| BspT107I | GGYRCC | 1 cut(s) | 400 |
| BspTNI | GGTCTC | 1 cut(s) | 68 |
| BsrFI | RCCGGY | 2 cut(s) | 276, 1725 |
| BsrI | ACTGG | 1 cut(s) | 1736 |
| BssAI | RCCGGY | 2 cut(s) | 276, 1725 |
| BssECI | CCNNGG | 2 cut(s) | 2052, 2151 |
| BssMI | GATC | 4 cut(s) | 975, 1038, 1571, 1803 |
| BssT1I | CCWWGG | 1 cut(s) | 2151 |
| Bst4CI | ACNGT | 6 cut(s) | 341, 1052, 1258, 1408, 1474, 1597 |
| Bst6I | CTCTTC | 3 cut(s) | 73, 921, 1299 |
| BstBAI | YACGTR | 1 cut(s) | 740 |
| BstC8I | GCNNGC | 7 cut(s) | 595, 717, 855, 1313, 1389, 1704, 2044 |
| BstDSI | CCRYGG | 1 cut(s) | 2151 |
| BstEII | GGTNACC | 1 cut(s) | 1849 |
| BstF5I | GGATG | 6 cut(s) | 24, 148, 241, 553, 1726, 2048 |
| BstHHI | GCGC | 1 cut(s) | 191 |
| BstKTI | GATC | 4 cut(s) | 978, 1041, 1574, 1806 |
| BstMAI | GTCTC | 3 cut(s) | 68, 267, 974 |
| BstMBI | GATC | 4 cut(s) | 975, 1038, 1571, 1803 |
| BstMCI | CGRYCG | 1 cut(s) | 277 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 167, 197, 206, 282, 794, 1613 |
| BstNSI | RCATGY | 3 cut(s) | 719, 2046, 2078 |
| BstPI | GGTNACC | 1 cut(s) | 1849 |
| BstSFI | CTRYAG | 3 cut(s) | 864, 1303, 1479 |
| BstV1I | GCAGC | 3 cut(s) | 706, 1246, 1302 |
| BstV2I | GAAGAC | 3 cut(s) | 500, 1110, 1598 |
| BstX2I | RGATCY | 1 cut(s) | 1571 |
| BstYI | RGATCY | 1 cut(s) | 1571 |
| BstZI | CGGCCG | 1 cut(s) | 274 |
| BsuI | GTATCC | 1 cut(s) | 1073 |
| BsuRI | GGCC | 8 cut(s) | 200, 276, 327, 758, 913, 1287, 1607, 1725 |
| BtgI | CCRYGG | 1 cut(s) | 2151 |
| BtrI | CACGTC | 1 cut(s) | 600 |
| BtsCI | GGATG | 6 cut(s) | 24, 148, 241, 553, 1726, 2048 |
| BtsIMutI | CAGTG | 1 cut(s) | 418 |
| Cac8I | GCNNGC | 7 cut(s) | 595, 717, 855, 1313, 1389, 1704, 2044 |
| CaiI | CAGNNNCTG | 2 cut(s) | 647, 1478 |
| CciI | TCATGA | 1 cut(s) | 51 |
| CfoI | GCGC | 1 cut(s) | 191 |
| Cfr10I | RCCGGY | 2 cut(s) | 276, 1725 |
| Cfr13I | GGNCC | 2 cut(s) | 198, 912 |
| CseI | GACGC | 1 cut(s) | 1925 |
| Csp6I | GTAC | 8 cut(s) | 13, 526, 741, 1066, 1454, 1729, 2148, 2164 |
| CviQI | GTAC | 8 cut(s) | 13, 526, 741, 1066, 1454, 1729, 2148, 2164 |
| DpnI | GATC | 4 cut(s) | 977, 1040, 1573, 1805 |
| DpnII | GATC | 4 cut(s) | 975, 1038, 1571, 1803 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 259 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 259 |
| EaeI | YGGCCR | 4 cut(s) | 274, 1285, 1605, 1723 |
| EagI | CGGCCG | 1 cut(s) | 274 |
| Eam1104I | CTCTTC | 3 cut(s) | 73, 921, 1299 |
| EarI | CTCTTC | 3 cut(s) | 73, 921, 1299 |
| Ecl136II | GAGCTC | 1 cut(s) | 6 |
| EclXI | CGGCCG | 1 cut(s) | 274 |
| Eco130I | CCWWGG | 1 cut(s) | 2151 |
| Eco24I | GRGCYC | 1 cut(s) | 8 |
| Eco31I | GGTCTC | 1 cut(s) | 68 |
| Eco32I | GATATC | 1 cut(s) | 610 |
| Eco52I | CGGCCG | 1 cut(s) | 274 |
| Eco53kI | GAGCTC | 1 cut(s) | 6 |
| Eco57I | CTGAAG | 7 cut(s) | 389, 408, 945, 1319, 1469, 1542, 1857 |
| Eco88I | CYCGRG | 1 cut(s) | 1685 |
| Eco91I | GGTNACC | 1 cut(s) | 1849 |
| EcoICRI | GAGCTC | 1 cut(s) | 6 |
| EcoO65I | GGTNACC | 1 cut(s) | 1849 |
| EcoRV | GATATC | 1 cut(s) | 610 |
| EcoT14I | CCWWGG | 1 cut(s) | 2151 |
| EcoT22I | ATGCAT | 1 cut(s) | 2080 |
| EcoT38I | GRGCYC | 1 cut(s) | 8 |
| ErhI | CCWWGG | 1 cut(s) | 2151 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1158, 1190 |
| FauI | CCCGC | 2 cut(s) | 80, 1311 |
| FauNDI | CATATG | 3 cut(s) | 885, 964, 1438 |
| Fnu4HI | GCNGC | 6 cut(s) | 201, 204, 207, 720, 1235, 1316 |
| FokI | GGATG | 6 cut(s) | 11, 135, 228, 540, 1733, 2035 |
| FriOI | GRGCYC | 1 cut(s) | 8 |
| Fsp4HI | GCNGC | 6 cut(s) | 201, 204, 207, 720, 1235, 1316 |
| FspBI | CTAG | 2 cut(s) | 1185, 1922 |
| GlaI | GCGC | 1 cut(s) | 190 |
| GluI | GCNGC | 6 cut(s) | 201, 204, 207, 720, 1235, 1316 |
| HaeIII | GGCC | 8 cut(s) | 200, 276, 327, 758, 913, 1287, 1607, 1725 |
| HapII | CCGG | 4 cut(s) | 228, 245, 277, 1726 |
| HgaI | GACGC | 1 cut(s) | 1925 |
| HhaI | GCGC | 1 cut(s) | 191 |
| Hin6I | GCGC | 1 cut(s) | 189 |
| HinP1I | GCGC | 1 cut(s) | 189 |
| HincII | GTYRAC | 2 cut(s) | 639, 652 |
| HindII | GTYRAC | 2 cut(s) | 639, 652 |
| HindIII | AAGCTT | 4 cut(s) | 130, 371, 851, 1331 |
| HinfI | GANTC | 7 cut(s) | 48, 644, 685, 1023, 1250, 1281, 1369 |
| HpaII | CCGG | 4 cut(s) | 228, 245, 277, 1726 |
| HphI | GGTGA | 5 cut(s) | 753, 1118, 1663, 1704, 2138 |
| Hpy166II | GTNNAC | 6 cut(s) | 15, 551, 639, 652, 1141, 1682 |
| Hpy188I | TCNGA | 6 cut(s) | 182, 604, 1022, 1206, 1263, 2053 |
| Hpy188III | TCNNGA | 7 cut(s) | 52, 245, 557, 979, 1036, 1430, 1835 |
| Hpy8I | GTNNAC | 6 cut(s) | 15, 551, 639, 652, 1141, 1682 |
| HpyAV | CCTTC | 9 cut(s) | 86, 135, 155, 289, 772, 1160, 1340, 1444, 1642 |
| HpyCH4III | ACNGT | 6 cut(s) | 341, 1052, 1258, 1408, 1474, 1597 |
| HpyCH4IV | ACGT | 4 cut(s) | 599, 739, 1228, 1959 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 167, 197, 206, 282, 794, 1613 |
| HpySE526I | ACGT | 4 cut(s) | 599, 739, 1228, 1959 |
| HspAI | GCGC | 1 cut(s) | 189 |
| Kpn2I | TCCGGA | 1 cut(s) | 244 |
| Kzo9I | GATC | 4 cut(s) | 975, 1038, 1571, 1803 |
| LmnI | GCTCC | 4 cut(s) | 11, 310, 1759, 2093 |
| Lsp1109I | GCAGC | 3 cut(s) | 706, 1246, 1302 |
| LweI | GCATC | 5 cut(s) | 250, 457, 1917, 1982, 2073 |
| MaeI | CTAG | 2 cut(s) | 1185, 1922 |
| MaeII | ACGT | 4 cut(s) | 599, 739, 1228, 1959 |
| MaeIII | GTNAC | 8 cut(s) | 698, 934, 967, 1238, 1474, 1710, 1849, 1945 |
| MalI | GATC | 4 cut(s) | 977, 1040, 1573, 1805 |
| MboI | GATC | 4 cut(s) | 975, 1038, 1571, 1803 |
| MflI | RGATCY | 1 cut(s) | 1571 |
| MhlI | GDGCHC | 2 cut(s) | 8, 1873 |
| MluCI | AATT | 9 cut(s) | 299, 406, 871, 1110, 1514, 1547, 1830, 1893, 2027 |
| MlyI | GAGTC | 1 cut(s) | 653 |
| MmeI | TCCRAC | 3 cut(s) | 249, 1258, 1549 |
| Mph1103I | ATGCAT | 1 cut(s) | 2080 |
| MroI | TCCGGA | 1 cut(s) | 244 |
| MroXI | GAANNNNTTC | 3 cut(s) | 223, 547, 2031 |
| MseI | TTAA | 6 cut(s) | 357, 462, 806, 1086, 1341, 1778 |
| MslI | CAYNNNNRTG | 2 cut(s) | 728, 1563 |
| MspI | CCGG | 4 cut(s) | 228, 245, 277, 1726 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 167, 197, 206, 282, 794, 1613 |
| NcoI | CCATGG | 1 cut(s) | 2151 |
| NdeI | CATATG | 3 cut(s) | 885, 964, 1438 |
| NdeII | GATC | 4 cut(s) | 975, 1038, 1571, 1803 |
| NlaIV | GGNNCC | 3 cut(s) | 306, 402, 1755 |
| NmuCI | GTSAC | 5 cut(s) | 698, 934, 1238, 1710, 1945 |
| NsiI | ATGCAT | 1 cut(s) | 2080 |
| NspI | RCATGY | 3 cut(s) | 719, 2046, 2078 |
| PaeI | GCATGC | 2 cut(s) | 719, 2046 |
| PaeR7I | CTCGAG | 1 cut(s) | 1685 |
| PagI | TCATGA | 1 cut(s) | 51 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1234 |
| PdmI | GAANNNNTTC | 3 cut(s) | 223, 547, 2031 |
| PfeI | GAWTC | 6 cut(s) | 48, 685, 1023, 1250, 1281, 1369 |
| PflFI | GACNNNGTC | 1 cut(s) | 643 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1721 |
| PkrI | GCNGC | 6 cut(s) | 202, 205, 208, 721, 1236, 1317 |
| PleI | GAGTC | 1 cut(s) | 652 |
| PpsI | GAGTC | 1 cut(s) | 652 |
| Ppu21I | YACGTR | 1 cut(s) | 740 |
| PshBI | ATTAAT | 1 cut(s) | 462 |
| Psp124BI | GAGCTC | 1 cut(s) | 8 |
| PspEI | GGTNACC | 1 cut(s) | 1849 |
| PspN4I | GGNNCC | 3 cut(s) | 306, 402, 1755 |
| PspPI | GGNCC | 2 cut(s) | 198, 912 |
| PspXI | VCTCGAGB | 1 cut(s) | 1685 |
| PstI | CTGCAG | 1 cut(s) | 1483 |
| PstNI | CAGNNNCTG | 2 cut(s) | 647, 1478 |
| PsuI | RGATCY | 1 cut(s) | 1571 |
| PsyI | GACNNNGTC | 1 cut(s) | 643 |
| RsaI | GTAC | 8 cut(s) | 14, 527, 742, 1067, 1455, 1730, 2149, 2165 |
| RsaNI | GTAC | 8 cut(s) | 13, 526, 741, 1066, 1454, 1729, 2148, 2164 |
| RseI | CAYNNNNRTG | 2 cut(s) | 728, 1563 |
| SacI | GAGCTC | 1 cut(s) | 8 |
| SaqAI | TTAA | 6 cut(s) | 357, 462, 806, 1086, 1341, 1778 |
| SatI | GCNGC | 6 cut(s) | 201, 204, 207, 720, 1235, 1316 |
| Sau3AI | GATC | 4 cut(s) | 975, 1038, 1571, 1803 |
| Sau96I | GGNCC | 2 cut(s) | 198, 912 |
| ScaI | AGTACT | 2 cut(s) | 527, 2165 |
| SchI | GAGTC | 1 cut(s) | 653 |
| SduI | GDGCHC | 2 cut(s) | 8, 1873 |
| SfaNI | GCATC | 5 cut(s) | 250, 457, 1917, 1982, 2073 |
| SfcI | CTRYAG | 3 cut(s) | 864, 1303, 1479 |
| Sfr274I | CTCGAG | 1 cut(s) | 1685 |
| SlaI | CTCGAG | 1 cut(s) | 1685 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 728, 1563 |
| SmlI | CTYRAG | 3 cut(s) | 575, 831, 1685 |
| SmoI | CTYRAG | 3 cut(s) | 575, 831, 1685 |
| SphI | GCATGC | 2 cut(s) | 719, 2046 |
| Sse9I | AATT | 9 cut(s) | 299, 406, 871, 1110, 1514, 1547, 1830, 1893, 2027 |
| SsiI | CCGC | 8 cut(s) | 73, 201, 204, 207, 518, 1063, 1318, 1967 |
| SspI | AATATT | 2 cut(s) | 619, 904 |
| SspMI | CTAG | 2 cut(s) | 1185, 1922 |
| SstI | GAGCTC | 1 cut(s) | 8 |
| StyI | CCWWGG | 1 cut(s) | 2151 |
| TaaI | ACNGT | 6 cut(s) | 341, 1052, 1258, 1408, 1474, 1597 |
| TaiI | ACGT | 4 cut(s) | 602, 742, 1231, 1962 |
| TaqI | TCGA | 6 cut(s) | 30, 46, 688, 978, 1041, 1686 |
| TasI | AATT | 9 cut(s) | 299, 406, 871, 1110, 1514, 1547, 1830, 1893, 2027 |
| TatI | WGTACW | 3 cut(s) | 12, 525, 2163 |
| TauI | GCSGC | 3 cut(s) | 203, 206, 209 |
| TfiI | GAWTC | 6 cut(s) | 48, 685, 1023, 1250, 1281, 1369 |
| Tru1I | TTAA | 6 cut(s) | 357, 462, 806, 1086, 1341, 1778 |
| Tru9I | TTAA | 6 cut(s) | 357, 462, 806, 1086, 1341, 1778 |
| TscAI | CASTG | 1 cut(s) | 418 |
| TseFI | GTSAC | 5 cut(s) | 698, 934, 1238, 1710, 1945 |
| TseI | GCWGC | 3 cut(s) | 719, 1234, 1315 |
| Tsp45I | GTSAC | 5 cut(s) | 698, 934, 1238, 1710, 1945 |
| TspDTI | ATGAA | 6 cut(s) | 40, 68, 137, 998, 1359, 2114 |
| TspGWI | ACGGA | 3 cut(s) | 231, 255, 1778 |
| TspRI | CASTG | 1 cut(s) | 418 |
| Tth111I | GACNNNGTC | 1 cut(s) | 643 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1721 |
| VspI | ATTAAT | 1 cut(s) | 462 |
| XapI | RAATTY | 3 cut(s) | 406, 1830, 2027 |
| XceI | RCATGY | 3 cut(s) | 719, 2046, 2078 |
| XhoI | CTCGAG | 1 cut(s) | 1685 |
| XmnI | GAANNNNTTC | 3 cut(s) | 223, 547, 2031 |
| XspI | CTAG | 2 cut(s) | 1185, 1922 |
| ZrmI | AGTACT | 2 cut(s) | 527, 2165 |
| Zsp2I | ATGCAT | 1 cut(s) | 2080 |