RchiOBHm_Chr7g0200901

Clp protease-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007687
Physical Location & Seq
Reverse (-)
18588354 .. 18591300
2947 bp
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UTR
Exon/CDS
Intron
PRQ17984

Sequence Viewer

Length: 480 bp
ATGGCTCGGAACCAACAGTACGTCTCGGAGTCAGAGCCCAACTGCCAAAGCTCCGACCTTTCTCTTCCACAATCTGCCTCAGCCTCCCGACCGCATGCTTCTGATTTTATCATTACAGAATCAAGGAGAGAGTTTCGTCCAATGAGGAAGTGCCCAAGAACCACTTTGGCTTCAAAATTTTTGCGGGCAAATGGACTTACAGTGTTCAAGGTGCGTGAAGAAACCATCAAGTTACTTGGGAAAGCTGATATGTACTTTTTCAGTCCTGAGCATCCTGCATTGACTGACGAAGCTCAAAGAGTGCTCGACTGGGCCGTTGATAAGAAAATAAAATCTGGTGGTAGTGGCGAAATTACAACATCTCATCTGCTTCTTGGCATTTGGTATGAAGTGGATTCACCGGGTCACAAGATAATGGCCTCCCTTGGCTTCAATGAAGAGAAAGTGAAAGAGCTGGAGTCTTTAATTTCTGATGGATGA

Protein Analysis

159

Amino Acids

17.82

Weight (kDa)

6.43

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 55 - 139 3.9e-07 Clp repeat (R) N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 92, 184
AcsI RAATTY 1 cut(s) 176
AfaI GTAC 2 cut(s) 20, 254
AgsI TTSAA 3 cut(s) 174, 208, 433
AluBI AGCT 4 cut(s) 51, 245, 293, 454
AluI AGCT 4 cut(s) 51, 245, 293, 454
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 28
AoxI GGCC 2 cut(s) 312, 417
ApoI RAATTY 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 312
AsuC2I CCSGG 1 cut(s) 402
AsuHPI GGTGA 1 cut(s) 390
BaeGI GKGCMC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 79
BccI CCATC 2 cut(s) 233, 467
BceAI ACGGC 1 cut(s) 299
BcnI CCSGG 1 cut(s) 402
BcoDI GTCTC 1 cut(s) 28
Bme1390I CCNGG 1 cut(s) 402
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 1 cut(s) 11
BmrFI CCNGG 1 cut(s) 402
BmrI ACTGGG 1 cut(s) 319
BmsI GCATC 1 cut(s) 280
BmuI ACTGGG 1 cut(s) 319
BpmI CTGGAG 1 cut(s) 476
Bpu10I CCTNAGC 2 cut(s) 79, 267
BpuMI CCSGG 1 cut(s) 402
BsaJI CCNNGG 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 314
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 1 cut(s) 271
BseMII CTCAG 2 cut(s) 93, 258
BseNI ACTGG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 155
Bsh1285I CGRYCG 1 cut(s) 92
BshFI GGCC 2 cut(s) 314, 419
BsiEI CGRYCG 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 28
BsmBI CGTCTC 1 cut(s) 28
BsnI GGCC 2 cut(s) 314, 419
Bsp1286I GDGCHC 3 cut(s) 39, 155, 306
BspACI CCGC 2 cut(s) 92, 184
BspANI GGCC 2 cut(s) 314, 419
BspCNI CTCAG 2 cut(s) 92, 259
BspLI GGNNCC 1 cut(s) 11
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 1 cut(s) 424
BssT1I CCWWGG 1 cut(s) 424
Bst4CI ACNGT 2 cut(s) 18, 202
Bst6I CTCTTC 2 cut(s) 69, 432
BstC8I GCNNGC 2 cut(s) 96, 186
BstDEI CTNAG 2 cut(s) 79, 267
BstF5I GGATG 1 cut(s) 271
BstMAI GTCTC 1 cut(s) 28
BstMCI CGRYCG 1 cut(s) 92
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 400
BstSLI GKGCMC 1 cut(s) 155
BsuRI GGCC 2 cut(s) 314, 419
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 2 cut(s) 96, 186
Cfr13I GGNCC 1 cut(s) 312
Csp6I GTAC 2 cut(s) 19, 253
CviAII CATG 1 cut(s) 95
CviQI GTAC 2 cut(s) 19, 253
DdeI CTNAG 2 cut(s) 79, 267
Eam1104I CTCTTC 2 cut(s) 69, 432
EarI CTCTTC 2 cut(s) 69, 432
Eco130I CCWWGG 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 424
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 1 cut(s) 98
FaiI YATR 3 cut(s) 96, 251, 387
FalI AAGNNNNNCTT 2 cut(s) 148, 180
FatI CATG 1 cut(s) 94
FauI CCCGC 1 cut(s) 177
FokI GGATG 1 cut(s) 258
FriOI GRGCYC 1 cut(s) 39
GsuI CTGGAG 1 cut(s) 476
HaeIII GGCC 2 cut(s) 314, 419
HapII CCGG 1 cut(s) 401
Hin1II CATG 1 cut(s) 98
HinfI GANTC 4 cut(s) 29, 119, 395, 458
HpaII CCGG 1 cut(s) 401
HphI GGTGA 1 cut(s) 390
Hpy188I TCNGA 6 cut(s) 9, 28, 34, 55, 103, 472
Hpy188III TCNNGA 2 cut(s) 87, 266
HpyCH4III ACNGT 2 cut(s) 18, 202
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 1 cut(s) 278
HpyF3I CTNAG 2 cut(s) 79, 267
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 1 cut(s) 98
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 6 cut(s) 279, 288, 295, 321, 414, 440
LweI GCATC 1 cut(s) 280
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 2 cut(s) 231, 404
MboII GAAGA 3 cut(s) 56, 230, 449
MhlI GDGCHC 3 cut(s) 39, 155, 306
MluCI AATT 3 cut(s) 176, 351, 465
MlyI GAGTC 2 cut(s) 38, 467
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 4 cut(s) 88, 94, 138, 430
MseI TTAA 1 cut(s) 464
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 1 cut(s) 402
NciI CCSGG 1 cut(s) 402
NlaIII CATG 1 cut(s) 98
NlaIV GGNNCC 1 cut(s) 11
NmuCI GTSAC 1 cut(s) 404
NspI RCATGY 1 cut(s) 98
PaeI GCATGC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PfeI GAWTC 2 cut(s) 119, 395
PleI GAGTC 2 cut(s) 37, 466
PpsI GAGTC 2 cut(s) 37, 466
PspN4I GGNNCC 1 cut(s) 11
PspPI GGNCC 1 cut(s) 312
PsrI GAACNNNNNNTAC 1 cut(s) 34
RsaI GTAC 2 cut(s) 20, 254
RsaNI GTAC 2 cut(s) 19, 253
SaqAI TTAA 1 cut(s) 464
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 2 cut(s) 38, 467
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 3 cut(s) 39, 155, 306
SetI ASST 7 cut(s) 24, 53, 60, 213, 247, 295, 456
SfaNI GCATC 1 cut(s) 280
SphI GCATGC 1 cut(s) 98
Sse9I AATT 3 cut(s) 176, 351, 465
SsiI CCGC 2 cut(s) 92, 184
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 2 cut(s) 18, 202
TaiI ACGT 1 cut(s) 24
TaqI TCGA 1 cut(s) 306
TasI AATT 3 cut(s) 176, 351, 465
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 2 cut(s) 119, 395
Tru1I TTAA 1 cut(s) 464
Tru9I TTAA 1 cut(s) 464
TscAI CASTG 1 cut(s) 207
TseFI GTSAC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 2 cut(s) 402, 450
TspRI CASTG 1 cut(s) 207
XapI RAATTY 1 cut(s) 176
XceI RCATGY 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.