RLG00000003721

Clp protease-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007687
Physical Location & Seq
Forward (+)
52043121 .. 52045206
2086 bp
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UTR
Exon/CDS
Intron
RLM00000003721

Sequence Viewer

Length: 564 bp
ATGGCCGCTCACTCCCTCTCCAAGCTTCCAGCTTTTGTCTCTGTCTTCAATCCCAATCAAACAACCAAACCCGAATCAATCAAGGAGAGAGTTTCGTCCAATGAGGAAGTGCCCAAATGGTCAAGCAGGGCTATAAAGTCGTTTGCCATGGGGGAATTGGAAGCCAGGAAGCTCAAGTACCCAACCACTGGGACTGAAGCAATTCTCATGGGTATCCTCATTGAGGGAACCACTTTGGCTTCAAAATTTTTGCGGGCAAATGGAGTTACGCTGTTCAAGGTGCGTGAAGAAACCATCAAGTTACTTGGGAAAGCTGATATGTACTTTTTCAGTCCTGAGCATCCTGCATTGACTGATGAAGCTCAAAGAGTGCTCGACTGGGCTGTTGATAAGAAAATAAAATCTGGTGGTAGTGGTGAAATTACAACTTCTCATCTGCTTCTTGGCATTTGGTATGAAGTGGATTCACCGGGTCACAAGATAATGGCCTCCCTTGGCTTCAATGAAGAGAAAGTGAAAGAGCTGGAGTCTTTAATTTCTGAACCTGGATTCATTGATGGGTGA

Protein Analysis

188

Amino Acids

20.63

Weight (kDa)

6.12

Isoelectric Point (pI)

29.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 41 - 162 1.4e-12 Clp repeat (R) N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 188
AccBSI CCGCTC 1 cut(s) 8
AciI CCGC 2 cut(s) 6, 253
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 216
AfaI GTAC 2 cut(s) 179, 323
AfiI CCNNNNNNNGG 2 cut(s) 188, 223
AgsI TTSAA 4 cut(s) 49, 243, 277, 502
AjnI CCWGG 2 cut(s) 164, 544
AluBI AGCT 6 cut(s) 25, 32, 172, 314, 362, 523
AluI AGCT 6 cut(s) 25, 32, 172, 314, 362, 523
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 1 cut(s) 43
AoxI GGCC 2 cut(s) 3, 486
ApoI RAATTY 1 cut(s) 245
Asp700I GAANNNNTTC 1 cut(s) 201
AsuC2I CCSGG 1 cut(s) 471
AsuHPI GGTGA 2 cut(s) 428, 459
BaeGI GKGCMC 1 cut(s) 114
BarI GAAGNNNNNNTAC 2 cut(s) 161, 193
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 375
BccI CCATC 2 cut(s) 302, 551
BciT130I CCWGG 2 cut(s) 166, 546
BciVI GTATCC 1 cut(s) 224
BcnI CCSGG 1 cut(s) 471
BcoDI GTCTC 1 cut(s) 43
BfuI GTATCC 1 cut(s) 224
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 3 cut(s) 166, 471, 546
BmiI GGNNCC 1 cut(s) 229
BmrFI CCNGG 3 cut(s) 166, 471, 546
BmrI ACTGGG 2 cut(s) 198, 388
BmsI GCATC 1 cut(s) 349
BmuI ACTGGG 2 cut(s) 198, 388
BpiI GAAGAC 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 545
Bpu10I CCTNAGC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 471
BsaJI CCNNGG 2 cut(s) 147, 493
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 223
Bse1I ACTGG 2 cut(s) 193, 383
BseBI CCWGG 2 cut(s) 166, 546
BseDI CCNNGG 2 cut(s) 147, 493
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 2 cut(s) 188, 223
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 2 cut(s) 193, 383
BseSI GKGCMC 1 cut(s) 114
BshFI GGCC 2 cut(s) 5, 488
BsiHKAI GWGCWC 1 cut(s) 375
BsiSI CCGG 1 cut(s) 470
BslFI GGGAC 1 cut(s) 205
BslI CCNNNNNNNGG 2 cut(s) 188, 223
BsmAI GTCTC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 205
BsnI GGCC 2 cut(s) 5, 488
Bsp1286I GDGCHC 2 cut(s) 114, 375
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 2 cut(s) 6, 253
BspANI GGCC 2 cut(s) 5, 488
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 229
BsrBI CCGCTC 1 cut(s) 8
BsrI ACTGG 2 cut(s) 193, 383
BssECI CCNNGG 2 cut(s) 147, 493
BssT1I CCWWGG 2 cut(s) 147, 493
Bst2UI CCWGG 2 cut(s) 166, 546
Bst6I CTCTTC 1 cut(s) 501
BstC8I GCNNGC 1 cut(s) 255
BstDEI CTNAG 1 cut(s) 336
BstDSI CCRYGG 1 cut(s) 147
BstENI CCTNNNNNAGG 1 cut(s) 221
BstF5I GGATG 1 cut(s) 340
BstMAI GTCTC 1 cut(s) 43
BstNI CCWGG 2 cut(s) 166, 546
BstSCI CCNGG 3 cut(s) 164, 469, 544
BstSLI GKGCMC 1 cut(s) 114
BstV2I GAAGAC 1 cut(s) 37
BsuI GTATCC 1 cut(s) 224
BsuRI GGCC 2 cut(s) 5, 488
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 255
Csp6I GTAC 2 cut(s) 178, 322
CviAII CATG 2 cut(s) 148, 208
CviQI GTAC 2 cut(s) 178, 322
DdeI CTNAG 1 cut(s) 336
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
Eco130I CCWWGG 2 cut(s) 147, 493
Eco57I CTGAAG 1 cut(s) 216
EcoNI CCTNNNNNAGG 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 164, 544
EcoT14I CCWWGG 2 cut(s) 147, 493
ErhI CCWWGG 2 cut(s) 147, 493
FaeI CATG 2 cut(s) 151, 211
FaiI YATR 5 cut(s) 134, 149, 209, 320, 456
FaqI GGGAC 1 cut(s) 205
FatI CATG 2 cut(s) 147, 207
FauI CCCGC 1 cut(s) 246
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 327
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 545
HaeIII GGCC 2 cut(s) 5, 488
HapII CCGG 1 cut(s) 470
Hin1II CATG 2 cut(s) 151, 211
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 4 cut(s) 74, 464, 527, 549
HpaII CCGG 1 cut(s) 470
HphI GGTGA 2 cut(s) 428, 459
Hpy188I TCNGA 1 cut(s) 541
Hpy188III TCNNGA 1 cut(s) 335
HpyCH4V TGCA 1 cut(s) 347
HpyF3I CTNAG 1 cut(s) 336
Hsp92II CATG 2 cut(s) 151, 211
LweI GCATC 1 cut(s) 349
MaeIII GTNAC 3 cut(s) 265, 300, 473
MbiI CCGCTC 1 cut(s) 8
MboII GAAGA 3 cut(s) 37, 299, 518
MhlI GDGCHC 2 cut(s) 114, 375
MluCI AATT 5 cut(s) 155, 201, 245, 420, 534
MlyI GAGTC 1 cut(s) 536
MnlI CCTC 5 cut(s) 26, 97, 217, 227, 499
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 1 cut(s) 533
MspI CCGG 1 cut(s) 470
MspR9I CCNGG 3 cut(s) 166, 471, 546
MvaI CCWGG 2 cut(s) 166, 546
NciI CCSGG 1 cut(s) 471
NcoI CCATGG 1 cut(s) 147
NlaIII CATG 2 cut(s) 151, 211
NlaIV GGNNCC 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 3 cut(s) 74, 464, 549
PflMI CCANNNNNTGG 1 cut(s) 188
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 535
PpsI GAGTC 1 cut(s) 535
Psp6I CCWGG 2 cut(s) 164, 544
PspGI CCWGG 2 cut(s) 164, 544
PspN4I GGNNCC 1 cut(s) 229
RsaI GTAC 2 cut(s) 179, 323
RsaNI GTAC 2 cut(s) 178, 322
SaqAI TTAA 1 cut(s) 533
SatI GCNGC 1 cut(s) 6
SchI GAGTC 1 cut(s) 536
ScrFI CCNGG 3 cut(s) 166, 471, 546
SduI GDGCHC 2 cut(s) 114, 375
SetI ASST 8 cut(s) 27, 34, 174, 282, 316, 364, 525, 547
SfaNI GCATC 1 cut(s) 349
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 5 cut(s) 155, 201, 245, 420, 534
SsiI CCGC 2 cut(s) 6, 253
StyD4I CCNGG 3 cut(s) 164, 469, 544
StyI CCWWGG 2 cut(s) 147, 493
TaqI TCGA 1 cut(s) 375
TasI AATT 5 cut(s) 155, 201, 245, 420, 534
TatI WGTACW 1 cut(s) 321
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 74, 464, 549
Tru1I TTAA 1 cut(s) 533
Tru9I TTAA 1 cut(s) 533
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 4 cut(s) 372, 471, 519, 541
TspRI CASTG 1 cut(s) 193
Van91I CCANNNNNTGG 1 cut(s) 188
XagI CCTNNNNNAGG 1 cut(s) 221
XapI RAATTY 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 154
XmnI GAANNNNTTC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.