Rw7G016320

Clp protease-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007687
Physical Location & Seq
Reverse (-)
16659155 .. 16663580
4426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016320.1

Sequence Viewer

Length: 705 bp
ATGGCCGCTCACTCCCTCTCCAAGCTTCCAGCTTTCGTCTATGCCTTCAATCCCAATCAAACAACCAAACCCGAATCGTGTTCTCTACCTCTGACCACGACTTTGAAGCCCACAAGTCTACAGAATCTATGGCTCGGAACCAACAGTCTCGGAGTCAGAGCCCAACTGCCAAAGCTCCGACCTTGCTCTTCCACAATCTGCCTCAGCCTCCCGACCGCAATCAAGGAGAGAGTTTGGTCCAATGAGGAAGTGCCCAAATGGTCTAGCAGGGCTATAAAGTCATTTGCCATGGGGGAATTGGAAGCCAGGAAGCTCAAGTACCCAACCACTGGGACTGAAGCAATTCTCATGGGTATCCTCATCGAGGGAACCACTTTGGCTTCAAAAATTTTGCGGGCAAATGGAGTTACATTGTTCAAGGTGCGTGAAGAAACCATCAAGTTACTTGGGAAAGCTGATATGTACTTTTTCAGTCCTGAGCATCCTGCATTGACTGACGAAGCTCAAAGAGTGCTCGACTGGGCTGTTGATAAGAAAATAAAATCTGGTGGTAGTGGTGAAATTACAACATCTCATCTGCTTCTTGGCATTTGGTATGAAGTGGATTCACCGGGTCACAAGATAATGGCCTCCCTTGGCTTCAATGAAGAGAAAGTGAAAGAGCTGGAGTCTTTAATTTCTGAACCTGGATTCATTGATGGATGA

Protein Analysis

234

Amino Acids

25.72

Weight (kDa)

8.35

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 88 - 209 6.3e-13 Clp repeat (R) N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 329
AccBSI CCGCTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 118
AciI CCGC 3 cut(s) 6, 216, 394
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 1 cut(s) 357
AfaI GTAC 2 cut(s) 320, 464
AfiI CCNNNNNNNGG 2 cut(s) 329, 364
AgsI TTSAA 5 cut(s) 49, 106, 384, 418, 643
AjnI CCWGG 2 cut(s) 305, 685
AluBI AGCT 7 cut(s) 25, 32, 175, 313, 455, 503, 664
AluI AGCT 7 cut(s) 25, 32, 175, 313, 455, 503, 664
Alw21I GWGCWC 1 cut(s) 516
Alw26I GTCTC 1 cut(s) 152
AoxI GGCC 2 cut(s) 3, 627
ApoI RAATTY 1 cut(s) 387
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
Asp700I GAANNNNTTC 1 cut(s) 342
AspS9I GGNCC 1 cut(s) 237
AsuC2I CCSGG 1 cut(s) 612
AsuHPI GGTGA 2 cut(s) 569, 600
AvaII GGWCC 1 cut(s) 237
BaeGI GKGCMC 1 cut(s) 255
BanII GRGCYC 1 cut(s) 163
BarI GAAGNNNNNNTAC 2 cut(s) 302, 334
Bbv12I GWGCWC 1 cut(s) 516
BbvCI CCTCAGC 1 cut(s) 203
BccI CCATC 2 cut(s) 443, 692
BciT130I CCWGG 2 cut(s) 307, 687
BciVI GTATCC 1 cut(s) 365
BcnI CCSGG 1 cut(s) 612
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 264
BfmI CTRYAG 1 cut(s) 119
BfuI GTATCC 1 cut(s) 365
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 3 cut(s) 307, 612, 687
Bme18I GGWCC 1 cut(s) 237
BmgT120I GGNCC 1 cut(s) 237
BmiI GGNNCC 2 cut(s) 139, 370
BmrFI CCNGG 3 cut(s) 307, 612, 687
BmrI ACTGGG 2 cut(s) 339, 529
BmsI GCATC 1 cut(s) 490
BmuI ACTGGG 2 cut(s) 339, 529
BpmI CTGGAG 1 cut(s) 686
Bpu10I CCTNAGC 2 cut(s) 203, 477
BpuEI CTTGAG 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 612
BsaJI CCNNGG 2 cut(s) 288, 634
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 364
Bse1I ACTGG 2 cut(s) 334, 524
BseBI CCWGG 2 cut(s) 307, 687
BseDI CCNNGG 2 cut(s) 288, 634
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 2 cut(s) 329, 364
BseMII CTCAG 2 cut(s) 217, 468
BseNI ACTGG 2 cut(s) 334, 524
BseSI GKGCMC 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 2 cut(s) 5, 629
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 516
BsiSI CCGG 1 cut(s) 611
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 2 cut(s) 329, 364
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 346
BsnI GGCC 2 cut(s) 5, 629
Bsp1286I GDGCHC 3 cut(s) 163, 255, 516
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 3 cut(s) 6, 216, 394
BspANI GGCC 2 cut(s) 5, 629
BspCNI CTCAG 2 cut(s) 216, 469
BspLI GGNNCC 2 cut(s) 139, 370
BspQI GCTCTTC 1 cut(s) 193
BsrBI CCGCTC 1 cut(s) 8
BsrI ACTGG 2 cut(s) 334, 524
BssECI CCNNGG 2 cut(s) 288, 634
BssT1I CCWWGG 2 cut(s) 288, 634
Bst2UI CCWGG 2 cut(s) 307, 687
Bst4CI ACNGT 1 cut(s) 146
Bst6I CTCTTC 2 cut(s) 193, 642
BstC8I GCNNGC 1 cut(s) 396
BstDEI CTNAG 2 cut(s) 203, 477
BstDSI CCRYGG 1 cut(s) 288
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 1 cut(s) 481
BstMAI GTCTC 1 cut(s) 152
BstMCI CGRYCG 1 cut(s) 216
BstNI CCWGG 2 cut(s) 307, 687
BstSCI CCNGG 3 cut(s) 305, 610, 685
BstSFI CTRYAG 1 cut(s) 119
BstSLI GKGCMC 1 cut(s) 255
BsuI GTATCC 1 cut(s) 365
BsuRI GGCC 2 cut(s) 5, 629
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 1 cut(s) 481
BtsIMutI CAGTG 1 cut(s) 327
Cac8I GCNNGC 1 cut(s) 396
Cfr13I GGNCC 1 cut(s) 237
Csp6I GTAC 2 cut(s) 319, 463
CviAII CATG 2 cut(s) 289, 349
CviQI GTAC 2 cut(s) 319, 463
DdeI CTNAG 2 cut(s) 203, 477
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 193, 642
EarI CTCTTC 2 cut(s) 193, 642
Eco130I CCWWGG 2 cut(s) 288, 634
Eco24I GRGCYC 1 cut(s) 163
Eco47I GGWCC 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 357
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 305, 685
EcoT14I CCWWGG 2 cut(s) 288, 634
EcoT38I GRGCYC 1 cut(s) 163
ErhI CCWWGG 2 cut(s) 288, 634
FaeI CATG 2 cut(s) 292, 352
FaiI YATR 7 cut(s) 42, 130, 275, 290, 350, 461, 597
FaqI GGGAC 1 cut(s) 346
FatI CATG 2 cut(s) 288, 348
FauI CCCGC 1 cut(s) 387
FblI GTMKAC 1 cut(s) 118
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 468
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 264
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 686
HaeIII GGCC 2 cut(s) 5, 629
HapII CCGG 1 cut(s) 611
Hin1II CATG 2 cut(s) 292, 352
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 6 cut(s) 74, 124, 153, 605, 668, 690
HpaII CCGG 1 cut(s) 611
HphI GGTGA 2 cut(s) 569, 600
Hpy166II GTNNAC 1 cut(s) 119
Hpy188I TCNGA 6 cut(s) 93, 137, 152, 158, 179, 682
Hpy188III TCNNGA 2 cut(s) 211, 476
Hpy8I GTNNAC 1 cut(s) 119
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4V TGCA 1 cut(s) 488
HpyF3I CTNAG 2 cut(s) 203, 477
Hsp92II CATG 2 cut(s) 292, 352
LguI GCTCTTC 1 cut(s) 193
LmnI GCTCC 1 cut(s) 180
LweI GCATC 1 cut(s) 490
MaeI CTAG 1 cut(s) 264
MaeIII GTNAC 3 cut(s) 406, 441, 614
MbiI CCGCTC 1 cut(s) 8
MboII GAAGA 3 cut(s) 180, 440, 659
MhlI GDGCHC 3 cut(s) 163, 255, 516
MluCI AATT 5 cut(s) 296, 342, 387, 561, 675
MlyI GAGTC 2 cut(s) 162, 677
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 8 cut(s) 26, 99, 212, 218, 238, 358, 368, 640
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 1 cut(s) 674
MspI CCGG 1 cut(s) 611
MspR9I CCNGG 3 cut(s) 307, 612, 687
MvaI CCWGG 2 cut(s) 307, 687
NciI CCSGG 1 cut(s) 612
NcoI CCATGG 1 cut(s) 288
NlaIII CATG 2 cut(s) 292, 352
NlaIV GGNNCC 2 cut(s) 139, 370
NmuCI GTSAC 1 cut(s) 614
PciSI GCTCTTC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 4 cut(s) 74, 124, 605, 690
PflMI CCANNNNNTGG 1 cut(s) 329
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 2 cut(s) 161, 676
PpsI GAGTC 2 cut(s) 161, 676
Psp6I CCWGG 2 cut(s) 305, 685
PspGI CCWGG 2 cut(s) 305, 685
PspN4I GGNNCC 2 cut(s) 139, 370
PspPI GGNCC 1 cut(s) 237
RsaI GTAC 2 cut(s) 320, 464
RsaNI GTAC 2 cut(s) 319, 463
SapI GCTCTTC 1 cut(s) 193
SaqAI TTAA 1 cut(s) 674
SatI GCNGC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 237
SchI GAGTC 2 cut(s) 162, 677
ScrFI CCNGG 3 cut(s) 307, 612, 687
SduI GDGCHC 3 cut(s) 163, 255, 516
SfaNI GCATC 1 cut(s) 490
SfcI CTRYAG 1 cut(s) 119
SinI GGWCC 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 5 cut(s) 296, 342, 387, 561, 675
SsiI CCGC 3 cut(s) 6, 216, 394
SspMI CTAG 1 cut(s) 264
StyD4I CCNGG 3 cut(s) 305, 610, 685
StyI CCWWGG 2 cut(s) 288, 634
TaaI ACNGT 1 cut(s) 146
TaqI TCGA 2 cut(s) 363, 516
TasI AATT 5 cut(s) 296, 342, 387, 561, 675
TatI WGTACW 1 cut(s) 462
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 4 cut(s) 74, 124, 605, 690
Tru1I TTAA 1 cut(s) 674
Tru9I TTAA 1 cut(s) 674
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 614
Tsp45I GTSAC 1 cut(s) 614
TspDTI ATGAA 3 cut(s) 612, 660, 682
TspRI CASTG 1 cut(s) 334
Van91I CCANNNNNTGG 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 237
XagI CCTNNNNNAGG 1 cut(s) 362
XapI RAATTY 1 cut(s) 387
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XmiI GTMKAC 1 cut(s) 118
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.