RchiOBHm_Chr7g0205571

Methyltransferase-like protein 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0015762
Physical Location & Seq
Reverse (-)
23308831 .. 23313150
4320 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18396

Sequence Viewer

Length: 651 bp
ATGCCAGCTGTCTTCTCTGCTTGTCATAGAGTACTCAAGGAGGTTCGTACAAGGCTGCCAGTTTTTTCTCCTGCTAAAGTGTTGGATTTTGGTGCTGGCACGAGTTCGGCTTTCTATCCTATTGTGATGGTTCATGCATATTCGAGGAGTAACTGGCTAAAGCAGATTCTTCACTGTTTTTATGTTCTAATCTTAGGTCAGAAGAACTTAACACTTATTCACAGTTATGCTACCCTCCAATCAATGACTAAAAGCATCAACAATTCAGAGAGAGAACATGACCTTGTAATTGCTATAAGTTGTGTTTATCTAGAACATCTGTCAATTACAAACTTAATTTTTCTCTTGGCATTTTTGGTCCTAATAATATCTGTTGTTTGTTACTATGGGTTGAAGTCCTATGTGCTTGGAGAGATACCATCGCTGAAGGATCGAATTACTGTAGTACGCCAACTTAGGGATCTTACGCGGGAAGTCCTGAAATGCCGTAAATCCAAGGCTGCAATTGATGAAACTTCCAAGGACTTGGTAGTGGCTAGAAAAAGTGGTGCCTATATAGTTGCTCCTTGCTCTCACGATGGGGCATGTCCTTTGGAGAAAGCAGGTAAATATTGTCATTTTGTTCAACGTTTGGAGAGAACTTCTACATAG

Protein Analysis

216

Amino Acids

24.29

Weight (kDa)

9.35

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rsm22 PF09243 133 - 215 2.3e-10 Mitochondrial small ribosomal subunit Rsm22
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 593
AccB1I GGYRCC 1 cut(s) 548
AccII CGCG 1 cut(s) 469
AciI CCGC 1 cut(s) 469
AclI AACGTT 1 cut(s) 628
AclWI GGATC 2 cut(s) 438, 468
AcuI CTGAAG 1 cut(s) 446
AfaI GTAC 3 cut(s) 33, 49, 447
AfiI CCNNNNNNNGG 1 cut(s) 457
AgsI TTSAA 2 cut(s) 394, 626
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
AlwI GGATC 2 cut(s) 438, 468
ApeKI GCWGC 2 cut(s) 55, 500
AspS9I GGNCC 1 cut(s) 358
AvaII GGWCC 1 cut(s) 358
BanI GGYRCC 1 cut(s) 548
BauI CACGAG 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 4
BbvI GCAGC 2 cut(s) 42, 487
BccI CCATC 3 cut(s) 121, 427, 572
BceAI ACGGC 1 cut(s) 471
BfaI CTAG 2 cut(s) 311, 537
BfmI CTRYAG 1 cut(s) 441
BfuAI ACCTGC 1 cut(s) 593
BisI GCNGC 2 cut(s) 56, 501
BlsI GCNGC 2 cut(s) 57, 502
BmcAI AGTACT 1 cut(s) 33
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 1 cut(s) 550
BmsI GCATC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 4
BpuEI CTTGAG 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 495, 519
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse1I ACTGG 2 cut(s) 59, 158
BseDI CCNNGG 2 cut(s) 495, 519
BseLI CCNNNNNNNGG 1 cut(s) 457
BseNI ACTGG 2 cut(s) 59, 158
BseRI GAGGAG 1 cut(s) 160
BseXI GCAGC 2 cut(s) 42, 487
Bsh1236I CGCG 1 cut(s) 469
BshNI GGYRCC 1 cut(s) 548
BslI CCNNNNNNNGG 1 cut(s) 457
Bsp143I GATC 2 cut(s) 430, 460
BspACI CCGC 1 cut(s) 469
BspFNI CGCG 1 cut(s) 469
BspLI GGNNCC 1 cut(s) 550
BspMI ACCTGC 1 cut(s) 593
BspPI GGATC 2 cut(s) 438, 468
BspT107I GGYRCC 1 cut(s) 548
BsrI ACTGG 2 cut(s) 59, 158
BssECI CCNNGG 2 cut(s) 495, 519
BssMI GATC 2 cut(s) 430, 460
BssSI CACGAG 1 cut(s) 100
BssT1I CCWWGG 2 cut(s) 495, 519
Bst2BI CACGAG 1 cut(s) 100
Bst4CI ACNGT 3 cut(s) 176, 224, 442
BstC8I GCNNGC 2 cut(s) 6, 97
BstDEI CTNAG 2 cut(s) 193, 455
BstFNI CGCG 1 cut(s) 469
BstKTI GATC 2 cut(s) 433, 463
BstMBI GATC 2 cut(s) 430, 460
BstNSI RCATGY 1 cut(s) 588
BstSFI CTRYAG 1 cut(s) 441
BstUI CGCG 1 cut(s) 469
BstV1I GCAGC 2 cut(s) 42, 487
BstV2I GAAGAC 1 cut(s) 4
BstX2I RGATCY 1 cut(s) 460
BstXI CCANNNNNNTGG 1 cut(s) 526
BstYI RGATCY 1 cut(s) 460
BtgZI GCGATG 1 cut(s) 405
BtsIMutI CAGTG 1 cut(s) 172
BveI ACCTGC 1 cut(s) 593
Cac8I GCNNGC 2 cut(s) 6, 97
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 3 cut(s) 32, 48, 446
CviAII CATG 3 cut(s) 134, 278, 585
CviJI RGCY 6 cut(s) 8, 55, 110, 157, 500, 536
CviKI_1 RGCY 6 cut(s) 8, 55, 110, 157, 500, 536
CviQI GTAC 3 cut(s) 32, 48, 446
DdeI CTNAG 2 cut(s) 193, 455
DpnI GATC 2 cut(s) 432, 462
DpnII GATC 2 cut(s) 430, 460
Eco130I CCWWGG 2 cut(s) 495, 519
Eco47I GGWCC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 446
EcoT14I CCWWGG 2 cut(s) 495, 519
EcoT22I ATGCAT 1 cut(s) 139
ErhI CCWWGG 2 cut(s) 495, 519
FaeI CATG 3 cut(s) 137, 281, 588
FatI CATG 3 cut(s) 133, 277, 584
FauI CCCGC 1 cut(s) 462
Fnu4HI GCNGC 2 cut(s) 56, 501
Fsp4HI GCNGC 2 cut(s) 56, 501
FspBI CTAG 2 cut(s) 311, 537
GluI GCNGC 2 cut(s) 56, 501
Hin1II CATG 3 cut(s) 137, 281, 588
HinfI GANTC 1 cut(s) 166
Hpy188I TCNGA 2 cut(s) 201, 268
Hpy188III TCNNGA 3 cut(s) 311, 478, 575
HpyAV CCTTC 1 cut(s) 421
HpyCH4III ACNGT 3 cut(s) 176, 224, 442
HpyCH4IV ACGT 1 cut(s) 628
HpyCH4V TGCA 2 cut(s) 137, 503
HpyF3I CTNAG 2 cut(s) 193, 455
HpySE526I ACGT 1 cut(s) 628
Hsp92II CATG 3 cut(s) 137, 281, 588
Kzo9I GATC 2 cut(s) 430, 460
LmnI GCTCC 1 cut(s) 568
LpnPI CCDG 7 cut(s) 18, 72, 81, 84, 139, 491, 588
Lsp1109I GCAGC 2 cut(s) 42, 487
LweI GCATC 1 cut(s) 264
MaeI CTAG 2 cut(s) 311, 537
MaeII ACGT 1 cut(s) 628
MaeIII GTNAC 2 cut(s) 149, 380
MalI GATC 2 cut(s) 432, 462
MboI GATC 2 cut(s) 430, 460
MboII GAAGA 3 cut(s) 4, 161, 214
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 1 cut(s) 460
MluCI AATT 6 cut(s) 262, 288, 324, 336, 435, 504
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 3 cut(s) 34, 138, 245
Mph1103I ATGCAT 1 cut(s) 139
MseI TTAA 2 cut(s) 209, 335
MslI CAYNNNNRTG 1 cut(s) 225
MspA1I CMGCKG 1 cut(s) 8
MunI CAATTG 1 cut(s) 504
MvnI CGCG 1 cut(s) 469
NdeII GATC 2 cut(s) 430, 460
NlaIII CATG 3 cut(s) 137, 281, 588
NlaIV GGNNCC 1 cut(s) 550
NsiI ATGCAT 1 cut(s) 139
NspI RCATGY 1 cut(s) 588
PfeI GAWTC 1 cut(s) 166
PkrI GCNGC 2 cut(s) 57, 502
Psp1406I AACGTT 1 cut(s) 628
PspN4I GGNNCC 1 cut(s) 550
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 3 cut(s) 33, 49, 447
RsaNI GTAC 3 cut(s) 32, 48, 446
RseI CAYNNNNRTG 1 cut(s) 225
SaqAI TTAA 2 cut(s) 209, 335
SatI GCNGC 2 cut(s) 56, 501
Sau3AI GATC 2 cut(s) 430, 460
Sau96I GGNCC 1 cut(s) 358
ScaI AGTACT 1 cut(s) 33
SetI ASST 6 cut(s) 10, 45, 199, 285, 607, 631
SfaNI GCATC 1 cut(s) 264
SfcI CTRYAG 1 cut(s) 441
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
Sse9I AATT 6 cut(s) 262, 288, 324, 336, 435, 504
SsiI CCGC 1 cut(s) 469
SspI AATATT 1 cut(s) 611
SspMI CTAG 2 cut(s) 311, 537
StyI CCWWGG 2 cut(s) 495, 519
TaaI ACNGT 3 cut(s) 176, 224, 442
TaiI ACGT 1 cut(s) 631
TaqI TCGA 2 cut(s) 143, 433
TasI AATT 6 cut(s) 262, 288, 324, 336, 435, 504
TatI WGTACW 1 cut(s) 31
TfiI GAWTC 1 cut(s) 166
Tru1I TTAA 2 cut(s) 209, 335
Tru9I TTAA 2 cut(s) 209, 335
TscAI CASTG 1 cut(s) 179
TseI GCWGC 2 cut(s) 55, 500
TspDTI ATGAA 2 cut(s) 122, 525
TspRI CASTG 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 358
XbaI TCTAGA 1 cut(s) 310
XceI RCATGY 1 cut(s) 588
XspI CTAG 2 cut(s) 311, 537
ZrmI AGTACT 1 cut(s) 33
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.