Rorug04G0088200

Methyltransferase-like protein 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0015762
Physical Location & Seq
Reverse (-)
13707239 .. 13707757
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0088200.1

Sequence Viewer

Length: 519 bp
ATGGATATGTTGGATGCATTGCATGTGGACCACCGTGACTTGTTTTTGGAGTGCATTTGGGCCATCTGGGCCGAGCGGAACAATGTAGTGTGGAAGAATGTCTCCTTCCAACCCATGAATTTGATTAGGTGGTCAGTCCGTGTCTTAGAGGAGTTTCAGAAATATCACCCGAAGGCAGTAAAAAAGAAGAAGAGGCCGCCGTCTAAGTGGCAGAATCCTCCCAGAGATAGGCTGAAAATAAATGTTGATGGTGTTTTCAGAGTTGATAGTGGATGTGGAGGCATTGGAGTAGTGGTCAGAAATGATTCTTGTACGGGTATTGCTGCGCTTGCTAGACCTTTTGTACATGCACACTCGGTTCTTAATATGGAAGTGGAGGCATGTAGAGCTGGTCTACTTTTTGGGATTCACCAAGGTTGGGCTGACATTGATATTGAAAGCAACTCTGCCATTCTGATTGCTGCTCTACAGAGTGAGGAGAAGAATTTCTCGGAGCCAGGTAACCTTGTTCGTAAATGA

Protein Analysis

172

Amino Acids

19.54

Weight (kDa)

8.73

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 81 - 171 1.1e-10 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 76
AccI GTMKAC 1 cut(s) 394
AciI CCGC 2 cut(s) 76, 197
AcsI RAATTY 2 cut(s) 118, 484
AfaI GTAC 2 cut(s) 313, 345
AfiI CCNNNNNNNGG 2 cut(s) 228, 418
AgsI TTSAA 1 cut(s) 437
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 1 cut(s) 389
AluI AGCT 1 cut(s) 389
Alw26I GTCTC 1 cut(s) 106
AoxI GGCC 3 cut(s) 60, 69, 194
ApeKI GCWGC 2 cut(s) 323, 461
ApoI RAATTY 2 cut(s) 118, 484
Asp700I GAANNNNTTC 2 cut(s) 304, 485
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 3 cut(s) 28, 60, 69
AsuHPI GGTGA 2 cut(s) 158, 401
AvaII GGWCC 1 cut(s) 28
BbvI GCAGC 2 cut(s) 310, 448
BccI CCATC 2 cut(s) 71, 242
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 1 cut(s) 498
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 1 cut(s) 467
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 3 cut(s) 197, 324, 462
BlsI GCNGC 3 cut(s) 198, 325, 463
Bme1390I CCNGG 1 cut(s) 498
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 3 cut(s) 28, 60, 69
BmiI GGNNCC 1 cut(s) 495
BmrFI CCNGG 1 cut(s) 498
BmsI GCATC 1 cut(s) 4
BsaJI CCNNGG 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 418
Bse3DI GCAATG 1 cut(s) 17
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 1 cut(s) 412
BseGI GGATG 2 cut(s) 19, 278
BseLI CCNNNNNNNGG 2 cut(s) 228, 418
BseMI GCAATG 1 cut(s) 17
BseRI GAGGAG 2 cut(s) 164, 491
BseXI GCAGC 2 cut(s) 310, 448
BshFI GGCC 3 cut(s) 62, 71, 196
BslI CCNNNNNNNGG 2 cut(s) 228, 418
BsmAI GTCTC 1 cut(s) 106
BsnI GGCC 3 cut(s) 62, 71, 196
Bsp1407I TGTACA 1 cut(s) 343
BspACI CCGC 2 cut(s) 76, 197
BspANI GGCC 3 cut(s) 62, 71, 196
BspLI GGNNCC 1 cut(s) 495
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 17
BsrGI TGTACA 1 cut(s) 343
BssECI CCNNGG 1 cut(s) 412
BssT1I CCWWGG 1 cut(s) 412
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 1 cut(s) 35
Bst6I CTCTTC 1 cut(s) 185
BstAUI TGTACA 1 cut(s) 343
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 2 cut(s) 145, 204
BstEII GGTNACC 1 cut(s) 500
BstF5I GGATG 2 cut(s) 19, 278
BstHHI GCGC 1 cut(s) 328
BstMAI GTCTC 1 cut(s) 106
BstMWI GCNNNNNNNGC 3 cut(s) 68, 329, 386
BstNI CCWGG 1 cut(s) 498
BstNSI RCATGY 3 cut(s) 26, 350, 384
BstPI GGTNACC 1 cut(s) 500
BstSCI CCNGG 1 cut(s) 496
BstSFI CTRYAG 1 cut(s) 467
BstV1I GCAGC 2 cut(s) 310, 448
BsuRI GGCC 3 cut(s) 62, 71, 196
BtsCI GGATG 2 cut(s) 19, 278
Cac8I GCNNGC 1 cut(s) 330
CfoI GCGC 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 28, 60, 69
Csp6I GTAC 2 cut(s) 312, 344
CviAII CATG 4 cut(s) 23, 115, 347, 381
CviJI RGCY 7 cut(s) 62, 71, 196, 232, 389, 422, 496
CviKI_1 RGCY 7 cut(s) 62, 71, 196, 232, 389, 422, 496
CviQI GTAC 2 cut(s) 312, 344
DdeI CTNAG 2 cut(s) 145, 204
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Eco130I CCWWGG 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 28
Eco91I GGTNACC 1 cut(s) 500
EcoO65I GGTNACC 1 cut(s) 500
EcoRII CCWGG 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 412
EcoT22I ATGCAT 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 412
FaeI CATG 4 cut(s) 26, 118, 350, 384
FaiI YATR 6 cut(s) 8, 24, 116, 348, 368, 382
FatI CATG 4 cut(s) 22, 114, 346, 380
FblI GTMKAC 1 cut(s) 394
Fnu4HI GCNGC 3 cut(s) 197, 324, 462
FokI GGATG 2 cut(s) 26, 285
Fsp4HI GCNGC 3 cut(s) 197, 324, 462
FspBI CTAG 1 cut(s) 333
GlaI GCGC 1 cut(s) 327
GluI GCNGC 3 cut(s) 197, 324, 462
HaeIII GGCC 3 cut(s) 62, 71, 196
HhaI GCGC 1 cut(s) 328
Hin1II CATG 4 cut(s) 26, 118, 350, 384
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HinfI GANTC 3 cut(s) 214, 305, 406
HphI GGTGA 2 cut(s) 158, 401
Hpy166II GTNNAC 2 cut(s) 28, 395
Hpy188I TCNGA 5 cut(s) 159, 260, 299, 456, 493
Hpy8I GTNNAC 2 cut(s) 28, 395
HpyAV CCTTC 2 cut(s) 115, 166
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4V TGCA 4 cut(s) 17, 22, 54, 350
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 329, 386
HpyF3I CTNAG 2 cut(s) 145, 204
Hsp92II CATG 4 cut(s) 26, 118, 350, 384
HspAI GCGC 1 cut(s) 326
LmnI GCTCC 1 cut(s) 493
LpnPI CCDG 5 cut(s) 52, 235, 375, 483, 510
Lsp1109I GCAGC 2 cut(s) 310, 448
LweI GCATC 1 cut(s) 4
MaeI CTAG 1 cut(s) 333
MaeIII GTNAC 2 cut(s) 35, 500
MbiI CCGCTC 1 cut(s) 76
MboII GAAGA 4 cut(s) 106, 199, 202, 493
MluCI AATT 2 cut(s) 118, 484
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 6 cut(s) 142, 186, 228, 272, 370, 469
Mph1103I ATGCAT 1 cut(s) 19
MroXI GAANNNNTTC 2 cut(s) 304, 485
MseI TTAA 1 cut(s) 363
MspR9I CCNGG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 498
MwoI GCNNNNNNNGC 3 cut(s) 68, 329, 386
NlaIII CATG 4 cut(s) 26, 118, 350, 384
NlaIV GGNNCC 1 cut(s) 495
NmeAIII GCCGAG 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 19
NspI RCATGY 3 cut(s) 26, 350, 384
PdmI GAANNNNTTC 2 cut(s) 304, 485
PfeI GAWTC 3 cut(s) 214, 305, 406
PkrI GCNGC 3 cut(s) 198, 325, 463
Psp6I CCWGG 1 cut(s) 496
PspEI GGTNACC 1 cut(s) 500
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 1 cut(s) 495
PspPI GGNCC 3 cut(s) 28, 60, 69
RsaI GTAC 2 cut(s) 313, 345
RsaNI GTAC 2 cut(s) 312, 344
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 3 cut(s) 197, 324, 462
Sau96I GGNCC 3 cut(s) 28, 60, 69
ScrFI CCNGG 1 cut(s) 498
SetI ASST 6 cut(s) 131, 340, 391, 418, 502, 507
SfaNI GCATC 1 cut(s) 4
SfcI CTRYAG 1 cut(s) 467
SfiI GGCCNNNNNGGCC 1 cut(s) 68
SinI GGWCC 1 cut(s) 28
Sse9I AATT 2 cut(s) 118, 484
SsiI CCGC 2 cut(s) 76, 197
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 496
StyI CCWWGG 1 cut(s) 412
TaaI ACNGT 1 cut(s) 35
TasI AATT 2 cut(s) 118, 484
TatI WGTACW 1 cut(s) 343
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 3 cut(s) 214, 305, 406
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 2 cut(s) 323, 461
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 1 cut(s) 131
TspGWI ACGGA 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 28
XapI RAATTY 2 cut(s) 118, 484
XceI RCATGY 3 cut(s) 26, 350, 384
XmiI GTMKAC 1 cut(s) 394
XmnI GAANNNNTTC 2 cut(s) 304, 485
XspI CTAG 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.