Rorug06G0435300

Methyltransferase-like protein 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0015762
Physical Location & Seq
Forward (+)
57005190 .. 57006788
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0435300.1

Sequence Viewer

Length: 1599 bp
ATGGCCGCCTTAACCAAATCAAACCTCCCTCTCCGCCGCTTTCTCTCTCCTCTCTCTCAACGAATTCTCAATCCCTCTTCTCTCTCTTCAGCTCCAGAGCCTCATCTCCAAGACGACGACGACACTTCCCAAACTGCCCCCAACTTAGCCACCGACGAAACCCTAATCGCCGACACATTCCACTCCCTAATCAAAGACCACCACCGCAACAACCCTAACGCTGACCCCAACCCTACTCCTCCAAACCCTACCTACACAATCCCTTCTCTCTCCTCCGACTTCTCCCAACTCTCCGCCGCCGCCTCCATTTCTCCGGCCGTCGTCCGCCGCGTCATCGAGAAATGCGGCGGCGTCCGCCACGGCATCCCCGTCCTCCAGGCCCTCGCCTTCTTCAACTGGGCCACGTCACGCGACGGCTTCGAGCACAGTCCGGGGCCGTACAACGAGATGGTCGACCTCGCCGGAAAGGTGAGGCAGTTCGATTTGGCTTGGCACGTGATCGACTTGATGAAAGCTCGGAATGTTGAAATTACGGTGGAGACATTCTCGATTCTTGTGCGCCGGTACGTGAGGGCCGGATTGGCTGCGGAGGCGGTGCACGCGTTTAACCGTATGGAGGAGTATGGCGTCAATCCTGATAGGATTGCTTTCTCGGTTGTGATTGGGATTCTCTGTAAGAAGAGAAGGGCAAGTGAAGCGCAGGCGTTTTTCGATAGCTTGAAGCATAAATTCGAGGCTGATGTGATTCTGTACACTAGCTTGGTTAATGGATGGTGTAGAGCTGGGAATATATCAGAGGCAGAGAGGGTGTTTAGGGAGATGAAGGCGGCGGGGATTGAGCCGAATGTATATAGTTACAGCATTGTGATAGATGCCTTGTGTAGGTGTGGACAGATTACTCGCGCTCATGATGTTTTCGCGGAGATGATTGATGCAGGTTGCAATCCCAACTCGATCACTTTTAACAATCTAATGAGAGTTCATGTGAAGGCTGGACGAACCGAAAAGGTGTTGCAGGTTTATAATCAGATGAAGAGGTTGGGATGCAATGCGGATTTGATTACTTACAATTTTCTTATAGAGTGTCATTGTAAGGATGAGAATGTTGAAGAGGCTGTTAAGGTGCTGAATTTGATGGTGAAGAAAGGCTGCAGCCCTAATGCATCGACTTTCAATCCCATATTTCGGTGCATTGCAAAGCTGAAGGATGTGAATGGTGCTCACAGGATGTACACTAAGATGAAGGATTTGGATTGCAAGGCGAACACAGTGACCTACAATGTGTTAATGCAGATGTTTGCGGAGTCAAAGTCGACTGATATGGTGCTCAAGCTGAAGAAGGAGATGGATGAGAATGAAGTGGAGCCTAATGTGAATACGTATAAGGTCTTAATCTCTATGTATTGTGCAATGGGGCATTGGAACAACGCTTACAGGTTCTGTAGGGAGATGATTGAGGAGAAATGCTTAAAACCAAGTATGCCGGTTTATGAGATGGTTCTGAAGCAGTTGAGGAATGCAGGACAGTTGAAGAAGCATGAGGAATTGGTGGAAAAAATGGTTGACAGGGGCTTCGTTACTCGCCCATTAGCAGTTTAG

Protein Analysis

532

Amino Acids

59.97

Weight (kDa)

8.25

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 147 - 189 9.6e-06 PPR repeat family
TPR_24 PF23276 151 - 241 3e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 166 - 224 5.7e-06 Pentatricopeptide repeat domain
PPR_long PF17177 235 - 339 5.4e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 243 - 292 7.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 245 - 275 9.2e-11 PPR repeat
PPR_2 PF13041 247 - 295 5.4e-17 PPR repeat family
PPR PF01535 250 - 279 1.5e-07 PPR repeat
PPR_1 PF12854 278 - 309 2.7e-09 PPR repeat
PPR PF01535 284 - 314 2.7e-06 PPR repeat
PPR_2 PF13041 289 - 326 5.7e-09 PPR repeat family
PPR_2 PF13041 316 - 361 1.7e-12 PPR repeat family
PPR PF01535 319 - 349 2.2e-06 PPR repeat
TPR_24 PF23276 331 - 445 1.3e-12 Fungal tetratrico peptide repeats
PPR_long PF17177 336 - 414 5.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 340 - 398 1.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 348 - 380 1.4e-09 PPR repeat
PPR_2 PF13041 352 - 400 6.4e-14 PPR repeat family
PPR PF01535 354 - 384 2.4e-07 PPR repeat
PPR_2 PF13041 386 - 434 2.8e-09 PPR repeat family
PPR_long PF17177 399 - 523 6.9e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 412 - 468 3.8e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 422 - 469 1.2e-11 PPR repeat family
PPR_3 PF13812 444 - 502 7.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 453 - 484 3.3e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
Acc36I ACCTGC 2 cut(s) 926, 1006
AccI GTMKAC 2 cut(s) 453, 1313
AccII CGCG 5 cut(s) 330, 411, 602, 903, 920
AcoI YGGCCR 2 cut(s) 3, 315
AcsI RAATTY 3 cut(s) 63, 728, 1129
AcuI CTGAAG 4 cut(s) 72, 1223, 1355, 1523
AcvI CACGTG 1 cut(s) 496
AcyI GRCGYC 2 cut(s) 351, 627
AfaI GTAC 4 cut(s) 440, 566, 752, 1232
AfiI CCNNNNNNNGG 3 cut(s) 616, 882, 1185
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 6 cut(s) 394, 527, 721, 1109, 1174, 1531
AjiI CACGTC 1 cut(s) 405
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 7 cut(s) 92, 515, 717, 759, 782, 1201, 1333
AluI AGCT 7 cut(s) 92, 515, 717, 759, 782, 1201, 1333
Alw21I GWGCWC 4 cut(s) 426, 600, 1222, 1329
Alw26I GTCTC 1 cut(s) 533
Alw44I GTGCAC 1 cut(s) 596
AlwNI CAGNNNCTG 1 cut(s) 1440
AoxI GGCC 6 cut(s) 3, 315, 378, 399, 434, 573
ApaLI GTGCAC 1 cut(s) 596
ApeKI GCWGC 3 cut(s) 584, 1149, 1152
ApoI RAATTY 3 cut(s) 63, 728, 1129
ArsI GACNNNNNNTTYG 2 cut(s) 1556, 1588
AspLEI GCGC 3 cut(s) 561, 700, 905
AspS9I GGNCC 4 cut(s) 379, 399, 434, 573
AsuC2I CCSGG 1 cut(s) 432
AsuHPI GGTGA 2 cut(s) 481, 1150
BaeGI GKGCMC 1 cut(s) 600
BbrPI CACGTG 1 cut(s) 496
Bbv12I GWGCWC 4 cut(s) 426, 600, 1222, 1329
BbvI GCAGC 3 cut(s) 571, 1136, 1164
BccI CCATC 5 cut(s) 442, 765, 1129, 1339, 1489
BceAI ACGGC 4 cut(s) 302, 376, 421, 430
BcgI CGANNNNNNTGC 2 cut(s) 538, 572
BciT130I CCWGG 1 cut(s) 377
BcnI CCSGG 1 cut(s) 432
BcoDI GTCTC 1 cut(s) 533
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 2 cut(s) 1150, 1441
BfuAI ACCTGC 2 cut(s) 926, 1006
BglI GCCNNNNNGGC 1 cut(s) 581
Bme1390I CCNGG 2 cut(s) 377, 432
BmgBI CACGTC 1 cut(s) 405
BmgT120I GGNCC 4 cut(s) 379, 399, 434, 573
BmiI GGNNCC 2 cut(s) 435, 1365
BmrFI CCNGG 2 cut(s) 377, 432
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 5 cut(s) 372, 862, 922, 1034, 1172
BmuI ACTGGG 1 cut(s) 406
BplI GAGNNNNNCTC 2 cut(s) 530, 562
BpmI CTGGAG 2 cut(s) 78, 359
BpuEI CTTGAG 1 cut(s) 1313
BpuMI CCSGG 1 cut(s) 432
BsaAI YACGTR 3 cut(s) 496, 568, 1380
BsaHI GRCGYC 2 cut(s) 351, 627
BsaJI CCNNGG 2 cut(s) 358, 431
BsaXI ACNNNNNCTCC 2 cut(s) 1295, 1325
Bsc4I CCNNNNNNNGG 3 cut(s) 616, 882, 1185
Bse118I RCCGGY 2 cut(s) 561, 1483
Bse1I ACTGG 1 cut(s) 401
Bse3DI GCAATG 3 cut(s) 1054, 1191, 1416
BseBI CCWGG 1 cut(s) 377
BseDI CCNNGG 2 cut(s) 358, 431
BseGI GGATG 7 cut(s) 363, 776, 1049, 1102, 1213, 1233, 1354
BseLI CCNNNNNNNGG 3 cut(s) 616, 882, 1185
BseMI GCAATG 3 cut(s) 1054, 1191, 1416
BseNI ACTGG 1 cut(s) 401
BseRI GAGGAG 5 cut(s) 39, 228, 262, 632, 1472
BseSI GKGCMC 1 cut(s) 600
BseX3I CGGCCG 1 cut(s) 315
BseXI GCAGC 3 cut(s) 571, 1136, 1164
BseYI CCCAGC 1 cut(s) 782
Bsh1236I CGCG 5 cut(s) 330, 411, 602, 903, 920
Bsh1285I CGRYCG 1 cut(s) 318
BshFI GGCC 6 cut(s) 5, 317, 380, 401, 436, 575
BsiEI CGRYCG 1 cut(s) 318
BsiHKAI GWGCWC 4 cut(s) 426, 600, 1222, 1329
BsiSI CCGG 6 cut(s) 314, 431, 462, 562, 576, 1484
BslI CCNNNNNNNGG 3 cut(s) 616, 882, 1185
BsmAI GTCTC 1 cut(s) 533
BsmI GAATGC 1 cut(s) 1522
BsnI GGCC 6 cut(s) 5, 317, 380, 401, 436, 575
Bsp1286I GDGCHC 4 cut(s) 426, 600, 1222, 1329
Bsp1407I TGTACA 2 cut(s) 750, 1230
Bsp143I GATC 2 cut(s) 498, 954
BspANI GGCC 6 cut(s) 5, 317, 380, 401, 436, 575
BspFNI CGCG 5 cut(s) 330, 411, 602, 903, 920
BspHI TCATGA 1 cut(s) 907
BspLI GGNNCC 2 cut(s) 435, 1365
BspMAI CTGCAG 1 cut(s) 1154
BspMI ACCTGC 2 cut(s) 926, 1006
BsrDI GCAATG 3 cut(s) 1054, 1191, 1416
BsrFI RCCGGY 2 cut(s) 561, 1483
BsrGI TGTACA 2 cut(s) 750, 1230
BsrI ACTGG 1 cut(s) 401
BssAI RCCGGY 2 cut(s) 561, 1483
BssECI CCNNGG 2 cut(s) 358, 431
BssMI GATC 2 cut(s) 498, 954
BssNI GRCGYC 2 cut(s) 351, 627
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 5 cut(s) 428, 535, 611, 1270, 1527
Bst6I CTCTTC 5 cut(s) 82, 91, 674, 1028, 1104
BstACI GRCGYC 2 cut(s) 351, 627
BstAUI TGTACA 2 cut(s) 750, 1230
BstBAI YACGTR 3 cut(s) 496, 568, 1380
BstC8I GCNNGC 2 cut(s) 600, 702
BstDEI CTNAG 2 cut(s) 145, 1236
BstDSI CCRYGG 1 cut(s) 358
BstENI CCTNNNNNAGG 1 cut(s) 880
BstF5I GGATG 7 cut(s) 363, 776, 1049, 1102, 1213, 1233, 1354
BstFNI CGCG 5 cut(s) 330, 411, 602, 903, 920
BstHHI GCGC 3 cut(s) 561, 700, 905
BstKTI GATC 2 cut(s) 501, 957
BstMAI GTCTC 1 cut(s) 533
BstMBI GATC 2 cut(s) 498, 954
BstMCI CGRYCG 1 cut(s) 318
BstMWI GCNNNNNNNGC 5 cut(s) 354, 581, 590, 599, 695
BstNI CCWGG 1 cut(s) 377
BstSCI CCNGG 2 cut(s) 375, 430
BstSFI CTRYAG 2 cut(s) 1150, 1441
BstSLI GKGCMC 1 cut(s) 600
BstSNI TACGTA 1 cut(s) 1380
BstUI CGCG 5 cut(s) 330, 411, 602, 903, 920
BstV1I GCAGC 3 cut(s) 571, 1136, 1164
BstZI CGGCCG 1 cut(s) 315
BsuRI GGCC 6 cut(s) 5, 317, 380, 401, 436, 575
BtgI CCRYGG 1 cut(s) 358
BtrI CACGTC 1 cut(s) 405
BtsCI GGATG 7 cut(s) 363, 776, 1049, 1102, 1213, 1233, 1354
BtsIMutI CAGTG 1 cut(s) 1275
BveI ACCTGC 2 cut(s) 926, 1006
Cac8I GCNNGC 2 cut(s) 600, 702
CaiI CAGNNNCTG 1 cut(s) 1440
CciI TCATGA 1 cut(s) 907
CfoI GCGC 3 cut(s) 561, 700, 905
Cfr10I RCCGGY 2 cut(s) 561, 1483
Cfr13I GGNCC 4 cut(s) 379, 399, 434, 573
CseI GACGC 3 cut(s) 319, 340, 616
Csp6I GTAC 4 cut(s) 439, 565, 751, 1231
CviAII CATG 3 cut(s) 908, 983, 1538
CviQI GTAC 4 cut(s) 439, 565, 751, 1231
DdeI CTNAG 2 cut(s) 145, 1236
DpnI GATC 2 cut(s) 500, 956
DpnII GATC 2 cut(s) 498, 954
EaeI YGGCCR 2 cut(s) 3, 315
EagI CGGCCG 1 cut(s) 315
Eam1104I CTCTTC 5 cut(s) 82, 91, 674, 1028, 1104
EarI CTCTTC 5 cut(s) 82, 91, 674, 1028, 1104
EciI GGCGGA 4 cut(s) 23, 283, 314, 344
EclXI CGGCCG 1 cut(s) 315
Eco105I TACGTA 1 cut(s) 1380
Eco52I CGGCCG 1 cut(s) 315
Eco57I CTGAAG 4 cut(s) 72, 1223, 1355, 1523
Eco72I CACGTG 1 cut(s) 496
EcoNI CCTNNNNNAGG 1 cut(s) 880
EcoO109I RGGNCCY 1 cut(s) 379
EcoRI GAATTC 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 375
EcoT22I ATGCAT 1 cut(s) 1165
FaeI CATG 3 cut(s) 911, 986, 1541
FatI CATG 3 cut(s) 907, 982, 1537
FauI CCCGC 1 cut(s) 823
FblI GTMKAC 2 cut(s) 453, 1313
FokI GGATG 7 cut(s) 350, 783, 1056, 1109, 1220, 1240, 1361
FspBI CTAG 1 cut(s) 756
GlaI GCGC 3 cut(s) 560, 699, 904
GsaI CCCAGC 1 cut(s) 786
GsuI CTGGAG 2 cut(s) 78, 359
HaeIII GGCC 6 cut(s) 5, 317, 380, 401, 436, 575
HapII CCGG 6 cut(s) 314, 431, 462, 562, 576, 1484
HgaI GACGC 3 cut(s) 319, 340, 616
HhaI GCGC 3 cut(s) 561, 700, 905
Hin1I GRCGYC 2 cut(s) 351, 627
Hin1II CATG 3 cut(s) 911, 986, 1541
Hin6I GCGC 3 cut(s) 559, 698, 903
HinP1I GCGC 3 cut(s) 559, 698, 903
HincII GTYRAC 3 cut(s) 454, 1314, 1564
HindII GTYRAC 3 cut(s) 454, 1314, 1564
HinfI GANTC 4 cut(s) 550, 667, 745, 1304
HpaII CCGG 6 cut(s) 314, 431, 462, 562, 576, 1484
HphI GGTGA 2 cut(s) 481, 1150
Hpy166II GTNNAC 7 cut(s) 454, 598, 753, 890, 1233, 1314, 1564
Hpy188I TCNGA 5 cut(s) 277, 519, 796, 1029, 1503
Hpy188III TCNNGA 5 cut(s) 95, 337, 547, 635, 908
Hpy8I GTNNAC 7 cut(s) 454, 598, 753, 890, 1233, 1314, 1564
Hpy99I CGWCG 5 cut(s) 119, 122, 158, 323, 416
HpyAV CCTTC 8 cut(s) 273, 397, 678, 817, 982, 1198, 1237, 1333
HpyCH4III ACNGT 5 cut(s) 428, 535, 611, 1270, 1527
HpyCH4IV ACGT 4 cut(s) 404, 495, 567, 1379
HpyF10VI GCNNNNNNNGC 5 cut(s) 354, 581, 590, 599, 695
HpyF3I CTNAG 2 cut(s) 145, 1236
HpySE526I ACGT 4 cut(s) 404, 495, 567, 1379
Hsp92I GRCGYC 2 cut(s) 351, 627
Hsp92II CATG 3 cut(s) 911, 986, 1541
HspAI GCGC 3 cut(s) 559, 698, 903
Kzo9I GATC 2 cut(s) 498, 954
LmnI GCTCC 2 cut(s) 97, 1363
Lsp1109I GCAGC 3 cut(s) 571, 1136, 1164
LweI GCATC 5 cut(s) 372, 862, 922, 1034, 1172
MaeI CTAG 1 cut(s) 756
MaeII ACGT 4 cut(s) 404, 495, 567, 1379
MaeIII GTNAC 4 cut(s) 405, 854, 1270, 1576
MalI GATC 2 cut(s) 500, 956
MboI GATC 2 cut(s) 498, 954
MboII GAAGA 9 cut(s) 69, 78, 382, 691, 1045, 1121, 1153, 1348, 1543
MhlI GDGCHC 4 cut(s) 426, 600, 1222, 1329
MluCI AATT 6 cut(s) 63, 528, 728, 1069, 1129, 1544
MluI ACGCGT 1 cut(s) 600
MlyI GAGTC 1 cut(s) 1313
MmeI TCCRAC 1 cut(s) 300
Mph1103I ATGCAT 1 cut(s) 1165
MseI TTAA 8 cut(s) 11, 606, 765, 963, 1119, 1286, 1391, 1469
MslI CAYNNNNRTG 1 cut(s) 1238
MspI CCGG 6 cut(s) 314, 431, 462, 562, 576, 1484
MspR9I CCNGG 2 cut(s) 377, 432
Mva1269I GAATGC 1 cut(s) 1522
MvaI CCWGG 1 cut(s) 377
MvnI CGCG 5 cut(s) 330, 411, 602, 903, 920
MwoI GCNNNNNNNGC 5 cut(s) 354, 581, 590, 599, 695
NciI CCSGG 1 cut(s) 432
NdeII GATC 2 cut(s) 498, 954
NlaIII CATG 3 cut(s) 911, 986, 1541
NlaIV GGNNCC 2 cut(s) 435, 1365
NmuCI GTSAC 2 cut(s) 405, 1270
NsiI ATGCAT 1 cut(s) 1165
PagI TCATGA 1 cut(s) 907
PcsI WCGNNNNNNNCGW 3 cut(s) 327, 366, 450
PctI GAATGC 1 cut(s) 1522
PfeI GAWTC 3 cut(s) 550, 667, 745
PleI GAGTC 1 cut(s) 1312
PmaCI CACGTG 1 cut(s) 496
PmlI CACGTG 1 cut(s) 496
PpsI GAGTC 1 cut(s) 1312
Ppu21I YACGTR 3 cut(s) 496, 568, 1380
PsiI TTATAA 1 cut(s) 1023
Psp6I CCWGG 1 cut(s) 375
PspCI CACGTG 1 cut(s) 496
PspFI CCCAGC 1 cut(s) 782
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 2 cut(s) 435, 1365
PspPI GGNCC 4 cut(s) 379, 399, 434, 573
PsrI GAACNNNNNNTAC 2 cut(s) 1415, 1447
PstI CTGCAG 1 cut(s) 1154
PstNI CAGNNNCTG 1 cut(s) 1440
RsaI GTAC 4 cut(s) 440, 566, 752, 1232
RsaNI GTAC 4 cut(s) 439, 565, 751, 1231
RseI CAYNNNNRTG 1 cut(s) 1238
SalI GTCGAC 2 cut(s) 452, 1312
SaqAI TTAA 8 cut(s) 11, 606, 765, 963, 1119, 1286, 1391, 1469
Sau3AI GATC 2 cut(s) 498, 954
Sau96I GGNCC 4 cut(s) 379, 399, 434, 573
SchI GAGTC 1 cut(s) 1313
ScrFI CCNGG 2 cut(s) 377, 432
SduI GDGCHC 4 cut(s) 426, 600, 1222, 1329
SfaNI GCATC 5 cut(s) 372, 862, 922, 1034, 1172
SfcI CTRYAG 2 cut(s) 1150, 1441
SmiMI CAYNNNNRTG 1 cut(s) 1238
SmlI CTYRAG 1 cut(s) 1328
SmoI CTYRAG 1 cut(s) 1328
SnaBI TACGTA 1 cut(s) 1380
Sse9I AATT 6 cut(s) 63, 528, 728, 1069, 1129, 1544
SspMI CTAG 1 cut(s) 756
StyD4I CCNGG 2 cut(s) 375, 430
TaaI ACNGT 5 cut(s) 428, 535, 611, 1270, 1527
TaiI ACGT 4 cut(s) 407, 498, 570, 1382
TasI AATT 6 cut(s) 63, 528, 728, 1069, 1129, 1544
TatI WGTACW 2 cut(s) 750, 1230
TauI GCSGC 8 cut(s) 8, 39, 299, 302, 330, 348, 351, 830
TfiI GAWTC 3 cut(s) 550, 667, 745
Tru1I TTAA 8 cut(s) 11, 606, 765, 963, 1119, 1286, 1391, 1469
Tru9I TTAA 8 cut(s) 11, 606, 765, 963, 1119, 1286, 1391, 1469
TscAI CASTG 1 cut(s) 1275
TseFI GTSAC 2 cut(s) 405, 1270
TseI GCWGC 3 cut(s) 584, 1149, 1152
Tsp45I GTSAC 2 cut(s) 405, 1270
TspDTI ATGAA 6 cut(s) 524, 836, 971, 1046, 1256, 1371
TspRI CASTG 1 cut(s) 1275
VneI GTGCAC 1 cut(s) 596
XagI CCTNNNNNAGG 1 cut(s) 880
XapI RAATTY 3 cut(s) 63, 728, 1129
XmiI GTMKAC 2 cut(s) 453, 1313
XspI CTAG 1 cut(s) 756
Zsp2I ATGCAT 1 cut(s) 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.