RchiOBHm_Chr7g0217791

DNA-damage-repair toleration protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0016457
Physical Location & Seq
Forward (+)
35713107 .. 35713667
561 bp
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UTR
Exon/CDS
Intron
PRQ19492

Sequence Viewer

Length: 561 bp
ATGAACTCAACCAAAATTCAAATCCCAGAAATTAAAAATCAAAATCAAATCCCAGCAGTGGCTCGCTCAACATCGACGTCGAGGACCTCGGCACTTCCTCCTACTACTTCATTGCCGTTGCGTCTCCTCCTCATCGCCAACACCAAAACTCGCGGACTTATCGCCTGCAGCACCGGCGTCGGCGTCACGACCTTAGCCAACAAGTTCCTCAAAGTCTTCGCGGCCACGTGTCTCTCCCTGTCTGGCGCCCTCAATGCCCAGCTCCAATGTCCTCCAGTCTCCTGCATGTCCACGGCGCCGGAGTCCACAATCGAGATCGTTGACACGTGGCTTAACACTCCCTTCAAAGTGCCATGTCCTGCGTTGAAGTTGAAACCTTGGCCGGAAATTGGAGCTGATTCTAATTTGAAGGAGAGCTCTTGTTCCATCTGCTGTTTGGTGAAGAATCAGAATTTGAATACGATTGATATAATTTCGGGTCATTCAATGAAGATACTGAGTTCTTTATCAAGTGGGATGGGAAATGGGATATGTTATTTGATGAAGATCTCAAGGCTGTGA

Protein Analysis

186

Amino Acids

19.79

Weight (kDa)

9.28

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LacAB_rpiB PF02502 43 - 116 2.4e-08 Ribose/Galactose Isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 80
AccB1I GGYRCC 2 cut(s) 245, 295
AccII CGCG 2 cut(s) 153, 221
AciI CCGC 2 cut(s) 153, 221
AcoI YGGCCR 2 cut(s) 222, 380
AcsI RAATTY 2 cut(s) 15, 451
AcvI CACGTG 2 cut(s) 228, 327
AcyI GRCGYC 5 cut(s) 77, 177, 183, 246, 296
AfiI CCNNNNNNNGG 2 cut(s) 58, 389
AflIII ACRYGT 2 cut(s) 227, 324
AgsI TTSAA 7 cut(s) 20, 346, 367, 373, 409, 457, 486
AluBI AGCT 3 cut(s) 262, 395, 417
AluI AGCT 3 cut(s) 262, 395, 417
Alw21I GWGCWC 1 cut(s) 419
Alw26I GTCTC 3 cut(s) 128, 236, 283
AoxI GGCC 2 cut(s) 222, 380
ApeKI GCWGC 1 cut(s) 168
ApoI RAATTY 2 cut(s) 15, 451
AspLEI GCGC 2 cut(s) 248, 298
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 451
AvaII GGWCC 1 cut(s) 84
BanI GGYRCC 2 cut(s) 245, 295
BanII GRGCYC 1 cut(s) 419
BbrPI CACGTG 2 cut(s) 228, 327
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 419
BbvI GCAGC 1 cut(s) 180
BccI CCATC 2 cut(s) 434, 511
BceAI ACGGC 2 cut(s) 100, 309
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BcoDI GTCTC 3 cut(s) 128, 236, 283
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 2 cut(s) 249, 299
BglII AGATCT 1 cut(s) 546
BisI GCNGC 2 cut(s) 169, 222
BlsI GCNGC 2 cut(s) 170, 223
Bme18I GGWCC 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 2 cut(s) 247, 297
BpiI GAAGAC 1 cut(s) 208
BpmI CTGGAG 1 cut(s) 258
Bpu10I CCTNAGC 1 cut(s) 193
BpuEI CTTGAG 1 cut(s) 535
BsaAI YACGTR 2 cut(s) 228, 327
BsaBI GATNNNNATC 1 cut(s) 545
BsaHI GRCGYC 5 cut(s) 77, 177, 183, 246, 296
BsaJI CCNNGG 3 cut(s) 87, 291, 377
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 389
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 275
Bse3DI GCAATG 1 cut(s) 110
Bse8I GATNNNNATC 1 cut(s) 545
BseDI CCNNGG 3 cut(s) 87, 291, 377
BseGI GGATG 1 cut(s) 522
BseJI GATNNNNATC 1 cut(s) 545
BseLI CCNNNNNNNGG 2 cut(s) 58, 389
BseMI GCAATG 1 cut(s) 110
BseMII CTCAG 1 cut(s) 488
BseNI ACTGG 1 cut(s) 275
BseRI GAGGAG 2 cut(s) 116, 119
BseXI GCAGC 1 cut(s) 180
BseYI CCCAGC 2 cut(s) 52, 258
Bsh1236I CGCG 2 cut(s) 153, 221
BshFI GGCC 2 cut(s) 224, 382
BshNI GGYRCC 2 cut(s) 245, 295
BsiHKAI GWGCWC 1 cut(s) 419
BsiSI CCGG 3 cut(s) 174, 299, 383
BslI CCNNNNNNNGG 2 cut(s) 58, 389
BsmAI GTCTC 3 cut(s) 128, 236, 283
BsmBI CGTCTC 1 cut(s) 128
BsnI GGCC 2 cut(s) 224, 382
Bsp1286I GDGCHC 1 cut(s) 419
Bsp143I GATC 2 cut(s) 315, 546
BspACI CCGC 2 cut(s) 153, 221
BspANI GGCC 2 cut(s) 224, 382
BspCNI CTCAG 1 cut(s) 489
BspFNI CGCG 2 cut(s) 153, 221
BspLI GGNNCC 2 cut(s) 247, 297
BspMAI CTGCAG 1 cut(s) 170
BspT107I GGYRCC 2 cut(s) 245, 295
BsrDI GCAATG 1 cut(s) 110
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 1 cut(s) 275
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 3 cut(s) 87, 291, 377
BssMI GATC 2 cut(s) 315, 546
BssNI GRCGYC 5 cut(s) 77, 177, 183, 246, 296
BssT1I CCWWGG 1 cut(s) 377
BstACI GRCGYC 5 cut(s) 77, 177, 183, 246, 296
BstBAI YACGTR 2 cut(s) 228, 327
BstC8I GCNNGC 2 cut(s) 64, 166
BstDEI CTNAG 2 cut(s) 193, 497
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 1 cut(s) 522
BstFNI CGCG 2 cut(s) 153, 221
BstH2I RGCGCY 2 cut(s) 249, 299
BstHHI GCGC 2 cut(s) 248, 298
BstKTI GATC 2 cut(s) 318, 549
BstMAI GTCTC 3 cut(s) 128, 236, 283
BstMBI GATC 2 cut(s) 315, 546
BstMWI GCNNNNNNNGC 2 cut(s) 174, 254
BstNSI RCATGY 1 cut(s) 289
BstSFI CTRYAG 1 cut(s) 166
BstUI CGCG 2 cut(s) 153, 221
BstV1I GCAGC 1 cut(s) 180
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 546
BstYI RGATCY 1 cut(s) 546
BsuRI GGCC 2 cut(s) 224, 382
BtgI CCRYGG 1 cut(s) 291
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 1 cut(s) 522
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 1 cut(s) 63
Cac8I GCNNGC 2 cut(s) 64, 166
CfoI GCGC 2 cut(s) 248, 298
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 84
CseI GACGC 3 cut(s) 110, 166, 172
CviAII CATG 2 cut(s) 286, 354
CviJI RGCY 9 cut(s) 62, 197, 224, 262, 331, 382, 395, 417, 556
CviKI_1 RGCY 9 cut(s) 62, 197, 224, 262, 331, 382, 395, 417, 556
DdeI CTNAG 2 cut(s) 193, 497
DinI GGCGCC 2 cut(s) 247, 297
DpnI GATC 2 cut(s) 317, 548
DpnII GATC 2 cut(s) 315, 546
EaeI YGGCCR 2 cut(s) 222, 380
Ecl136II GAGCTC 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 377
Eco24I GRGCYC 1 cut(s) 419
Eco47I GGWCC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 417
Eco72I CACGTG 2 cut(s) 228, 327
EcoICRI GAGCTC 1 cut(s) 417
EcoO109I RGGNCCY 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 377
EcoT38I GRGCYC 1 cut(s) 419
EgeI GGCGCC 2 cut(s) 247, 297
EheI GGCGCC 2 cut(s) 247, 297
ErhI CCWWGG 1 cut(s) 377
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 2 cut(s) 289, 357
FaiI YATR 4 cut(s) 287, 355, 470, 532
FatI CATG 2 cut(s) 285, 353
Fnu4HI GCNGC 2 cut(s) 169, 222
FokI GGATG 1 cut(s) 529
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 2 cut(s) 169, 222
GlaI GCGC 2 cut(s) 247, 297
GluI GCNGC 2 cut(s) 169, 222
GsaI CCCAGC 2 cut(s) 56, 262
GsuI CTGGAG 1 cut(s) 258
HaeII RGCGCY 2 cut(s) 249, 299
HaeIII GGCC 2 cut(s) 224, 382
HapII CCGG 3 cut(s) 174, 299, 383
HgaI GACGC 3 cut(s) 110, 166, 172
HhaI GCGC 2 cut(s) 248, 298
Hin1I GRCGYC 5 cut(s) 77, 177, 183, 246, 296
Hin1II CATG 2 cut(s) 289, 357
Hin6I GCGC 2 cut(s) 246, 296
HinP1I GCGC 2 cut(s) 246, 296
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 3 cut(s) 302, 398, 445
HpaII CCGG 3 cut(s) 174, 299, 383
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 3 cut(s) 291, 306, 322
Hpy188I TCNGA 1 cut(s) 450
Hpy188III TCNNGA 2 cut(s) 187, 313
Hpy8I GTNNAC 3 cut(s) 291, 306, 322
Hpy99I CGWCG 3 cut(s) 79, 82, 182
HpyAV CCTTC 2 cut(s) 352, 403
HpyCH4IV ACGT 3 cut(s) 77, 227, 326
HpyCH4V TGCA 2 cut(s) 168, 285
HpyF10VI GCNNNNNNNGC 2 cut(s) 174, 254
HpyF3I CTNAG 2 cut(s) 193, 497
HpySE526I ACGT 3 cut(s) 77, 227, 326
Hsp92I GRCGYC 5 cut(s) 77, 177, 183, 246, 296
Hsp92II CATG 2 cut(s) 289, 357
HspAI GCGC 2 cut(s) 246, 296
KasI GGCGCC 2 cut(s) 245, 295
Kzo9I GATC 2 cut(s) 315, 546
LmnI GCTCC 2 cut(s) 267, 392
Lsp1109I GCAGC 1 cut(s) 180
MaeII ACGT 3 cut(s) 77, 227, 326
MaeIII GTNAC 1 cut(s) 184
MalI GATC 2 cut(s) 317, 548
MboI GATC 2 cut(s) 315, 546
MboII GAAGA 4 cut(s) 208, 454, 502, 556
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 1 cut(s) 419
MluCI AATT 6 cut(s) 15, 30, 387, 403, 451, 471
Mly113I GGCGCC 2 cut(s) 246, 296
MlyI GAGTC 1 cut(s) 311
MnlI CCTC 8 cut(s) 75, 97, 108, 137, 140, 218, 260, 282
MseI TTAA 2 cut(s) 33, 333
MspI CCGG 3 cut(s) 174, 299, 383
MvnI CGCG 2 cut(s) 153, 221
MwoI GCNNNNNNNGC 2 cut(s) 174, 254
NarI GGCGCC 2 cut(s) 246, 296
NdeII GATC 2 cut(s) 315, 546
NlaIII CATG 2 cut(s) 289, 357
NlaIV GGNNCC 2 cut(s) 247, 297
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 1 cut(s) 184
NspI RCATGY 1 cut(s) 289
PfeI GAWTC 2 cut(s) 398, 445
PkrI GCNGC 2 cut(s) 170, 223
PleI GAGTC 1 cut(s) 310
PluTI GGCGCC 2 cut(s) 249, 299
PmaCI CACGTG 2 cut(s) 228, 327
PmlI CACGTG 2 cut(s) 228, 327
PpsI GAGTC 1 cut(s) 310
Ppu21I YACGTR 2 cut(s) 228, 327
PpuMI RGGWCCY 1 cut(s) 84
Psp124BI GAGCTC 1 cut(s) 419
Psp5II RGGWCCY 1 cut(s) 84
PspCI CACGTG 2 cut(s) 228, 327
PspFI CCCAGC 2 cut(s) 52, 258
PspN4I GGNNCC 2 cut(s) 247, 297
PspPI GGNCC 1 cut(s) 84
PspPPI RGGWCCY 1 cut(s) 84
PstI CTGCAG 1 cut(s) 170
PsuI RGATCY 1 cut(s) 546
SacI GAGCTC 1 cut(s) 419
SaqAI TTAA 2 cut(s) 33, 333
SatI GCNGC 2 cut(s) 169, 222
Sau3AI GATC 2 cut(s) 315, 546
Sau96I GGNCC 1 cut(s) 84
SchI GAGTC 1 cut(s) 311
SduI GDGCHC 1 cut(s) 419
SetI ASST 9 cut(s) 80, 89, 194, 230, 264, 329, 379, 397, 419
SfcI CTRYAG 1 cut(s) 166
SfoI GGCGCC 2 cut(s) 247, 297
SgrAI CRCCGGYG 1 cut(s) 173
SinI GGWCC 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
Sse9I AATT 6 cut(s) 15, 30, 387, 403, 451, 471
SsiI CCGC 2 cut(s) 153, 221
SspDI GGCGCC 2 cut(s) 245, 295
SstI GAGCTC 1 cut(s) 419
StyI CCWWGG 1 cut(s) 377
TaiI ACGT 3 cut(s) 80, 230, 329
TaqI TCGA 3 cut(s) 74, 80, 312
TasI AATT 6 cut(s) 15, 30, 387, 403, 451, 471
TauI GCSGC 1 cut(s) 224
TfiI GAWTC 2 cut(s) 398, 445
Tru1I TTAA 2 cut(s) 33, 333
Tru9I TTAA 2 cut(s) 33, 333
TscAI CASTG 1 cut(s) 63
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 1 cut(s) 168
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 4 cut(s) 17, 99, 503, 557
TspRI CASTG 1 cut(s) 63
VpaK11BI GGWCC 1 cut(s) 84
XapI RAATTY 2 cut(s) 15, 451
XceI RCATGY 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
ZraI GACGTC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.