Rh4CG138300

DNA-damage-repair toleration protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016457
Physical Location & Seq
Forward (+)
27448155 .. 27448760
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG138300.1

Sequence Viewer

Length: 606 bp
ATGAACTCAACCAAAATTCACATCCCAGAAATTAAAAATCAAAATCAAATCCCAGCAGTGGCTCGCTCAACATCGACGTCGAGGACCTCGACACTTCCTCCTACTACTCCATCACCGTTGCGTCTCCTCCTCGCCGCCAACACCAAAACTCGGGGACTTATCGCCTGCAGCACCGGCGTCGACGTCGGCGTCTCGACCTCAGCCAACAAGTTCCCCGAAGTCTTCGTGGCCACGTGTCTCTCCCTGTCCGACGCCCTCAACGCCCAGCTCCAACGTCCTCCTATCTCTGGCATGTCCACGGCGCCGAAATCCGCCATCGAGATCGTCGACACGTGGCTCAACATTCCCTTCAAAGTGCCCTGTCCTGCTTTGAAGTCGAAACCTTGGCCGGAAATTGGAGCTGATTCTGATTTGAAGGAGAGCTCTTGTTCCATCTGCTGTTTGGCGAACAATCGGAATTTGAATCCGATTGATATAATTTCAGGTCATTCAATGAAGATATTGAGTTTTTTATCAAGTGGGATGGGAAATGGGATATGTTATTTGATGAAGATCTCAAGGCTGCGAACAAGGCTATATGATCAAGAAATTGGAGAATGGGGGTAA

Protein Analysis

201

Amino Acids

21.64

Weight (kDa)

9.05

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LacAB_rpiB PF02502 42 - 118 3.3e-06 Ribose/Galactose Isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AatII GACGTC 2 cut(s) 80, 186
AccB1I GGYRCC 1 cut(s) 301
AccI GTMKAC 2 cut(s) 180, 327
AciI CCGC 2 cut(s) 135, 312
AcoI YGGCCR 2 cut(s) 228, 386
AcsI RAATTY 2 cut(s) 15, 457
AcvI CACGTG 2 cut(s) 234, 333
AcyI GRCGYC 6 cut(s) 77, 177, 183, 189, 252, 302
AfiI CCNNNNNNNGG 4 cut(s) 58, 150, 287, 395
AflIII ACRYGT 2 cut(s) 233, 330
AgsI TTSAA 5 cut(s) 352, 373, 415, 463, 492
AluBI AGCT 3 cut(s) 268, 401, 423
AluI AGCT 3 cut(s) 268, 401, 423
Alw21I GWGCWC 1 cut(s) 425
Alw26I GTCTC 3 cut(s) 128, 196, 242
Ama87I CYCGRG 1 cut(s) 150
AoxI GGCC 2 cut(s) 228, 386
ApeKI GCWGC 2 cut(s) 168, 562
ApoI RAATTY 2 cut(s) 15, 457
AspLEI GCGC 1 cut(s) 304
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 105
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 1 cut(s) 84
BaeGI GKGCMC 1 cut(s) 360
BalI TGGCCA 1 cut(s) 230
BanI GGYRCC 1 cut(s) 301
BanII GRGCYC 1 cut(s) 425
BbrPI CACGTG 2 cut(s) 234, 333
BbsI GAAGAC 1 cut(s) 214
Bbv12I GWGCWC 1 cut(s) 425
BbvCI CCTCAGC 1 cut(s) 199
BbvI GCAGC 2 cut(s) 180, 549
BccI CCATC 4 cut(s) 118, 323, 440, 517
BceAI ACGGC 1 cut(s) 315
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BclI TGATCA 1 cut(s) 580
BcoDI GTCTC 3 cut(s) 128, 196, 242
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 1 cut(s) 305
BglII AGATCT 1 cut(s) 552
BisI GCNGC 3 cut(s) 135, 169, 563
BlsI GCNGC 3 cut(s) 136, 170, 564
Bme18I GGWCC 1 cut(s) 84
BmeT110I CYCGRG 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 303
BpiI GAAGAC 1 cut(s) 214
Bpu10I CCTNAGC 1 cut(s) 199
BpuEI CTTGAG 1 cut(s) 541
BsaAI YACGTR 2 cut(s) 234, 333
BsaBI GATNNNNATC 1 cut(s) 551
BsaHI GRCGYC 6 cut(s) 77, 177, 183, 189, 252, 302
BsaJI CCNNGG 2 cut(s) 297, 383
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 150, 287, 395
Bse118I RCCGGY 1 cut(s) 173
Bse8I GATNNNNATC 1 cut(s) 551
BseDI CCNNGG 2 cut(s) 297, 383
BseGI GGATG 2 cut(s) 21, 528
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 4 cut(s) 58, 150, 287, 395
BseMII CTCAG 1 cut(s) 213
BseRI GAGGAG 2 cut(s) 116, 119
BseSI GKGCMC 1 cut(s) 360
BseXI GCAGC 2 cut(s) 180, 549
BseYI CCCAGC 2 cut(s) 52, 264
BshFI GGCC 2 cut(s) 230, 388
BshNI GGYRCC 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 425
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 2 cut(s) 174, 389
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 4 cut(s) 58, 150, 287, 395
BsmAI GTCTC 3 cut(s) 128, 196, 242
BsmBI CGTCTC 2 cut(s) 128, 196
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 2 cut(s) 230, 388
BsoBI CYCGRG 1 cut(s) 150
Bsp1286I GDGCHC 2 cut(s) 360, 425
Bsp143I GATC 3 cut(s) 321, 552, 580
BspACI CCGC 2 cut(s) 135, 312
BspANI GGCC 2 cut(s) 230, 388
BspCNI CTCAG 1 cut(s) 212
BspLI GGNNCC 1 cut(s) 303
BspMAI CTGCAG 1 cut(s) 170
BspT107I GGYRCC 1 cut(s) 301
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 297, 383
BssMI GATC 3 cut(s) 321, 552, 580
BssNI GRCGYC 6 cut(s) 77, 177, 183, 189, 252, 302
BssT1I CCWWGG 1 cut(s) 383
Bst4CI ACNGT 1 cut(s) 117
BstACI GRCGYC 6 cut(s) 77, 177, 183, 189, 252, 302
BstBAI YACGTR 2 cut(s) 234, 333
BstC8I GCNNGC 2 cut(s) 64, 166
BstDEI CTNAG 1 cut(s) 199
BstDSI CCRYGG 1 cut(s) 297
BstF5I GGATG 2 cut(s) 21, 528
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 3 cut(s) 324, 555, 583
BstMAI GTCTC 3 cut(s) 128, 196, 242
BstMBI GATC 3 cut(s) 321, 552, 580
BstMWI GCNNNNNNNGC 3 cut(s) 174, 260, 571
BstNSI RCATGY 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 166
BstSLI GKGCMC 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 180, 549
BstV2I GAAGAC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 552
BstYI RGATCY 1 cut(s) 552
BsuRI GGCC 2 cut(s) 230, 388
BtgI CCRYGG 1 cut(s) 297
BtsCI GGATG 2 cut(s) 21, 528
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 1 cut(s) 63
Cac8I GCNNGC 2 cut(s) 64, 166
CfoI GCGC 1 cut(s) 304
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 84
CseI GACGC 4 cut(s) 110, 166, 178, 260
CviAII CATG 1 cut(s) 292
DdeI CTNAG 1 cut(s) 199
DinI GGCGCC 1 cut(s) 303
DpnI GATC 3 cut(s) 323, 554, 582
DpnII GATC 3 cut(s) 321, 552, 580
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 2 cut(s) 228, 386
EciI GGCGGA 1 cut(s) 301
Ecl136II GAGCTC 1 cut(s) 423
Eco130I CCWWGG 1 cut(s) 383
Eco24I GRGCYC 1 cut(s) 425
Eco47I GGWCC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 423
Eco72I CACGTG 2 cut(s) 234, 333
Eco88I CYCGRG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 423
EcoO109I RGGNCCY 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 383
EcoT38I GRGCYC 1 cut(s) 425
EgeI GGCGCC 1 cut(s) 303
EheI GGCGCC 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 383
Esp3I CGTCTC 2 cut(s) 128, 196
FaeI CATG 1 cut(s) 295
FaiI YATR 5 cut(s) 293, 476, 538, 577, 579
FaqI GGGAC 1 cut(s) 168
FatI CATG 1 cut(s) 291
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 2 cut(s) 180, 327
Fnu4HI GCNGC 3 cut(s) 135, 169, 563
FokI GGATG 2 cut(s) 8, 535
FriOI GRGCYC 1 cut(s) 425
Fsp4HI GCNGC 3 cut(s) 135, 169, 563
GlaI GCGC 1 cut(s) 303
GluI GCNGC 3 cut(s) 135, 169, 563
GsaI CCCAGC 2 cut(s) 56, 268
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 2 cut(s) 230, 388
HapII CCGG 2 cut(s) 174, 389
HgaI GACGC 4 cut(s) 110, 166, 178, 260
HhaI GCGC 1 cut(s) 304
Hin1I GRCGYC 6 cut(s) 77, 177, 183, 189, 252, 302
Hin1II CATG 1 cut(s) 295
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HincII GTYRAC 2 cut(s) 181, 328
HindII GTYRAC 2 cut(s) 181, 328
HinfI GANTC 2 cut(s) 404, 463
HpaII CCGG 2 cut(s) 174, 389
HphI GGTGA 1 cut(s) 105
Hpy166II GTNNAC 3 cut(s) 181, 297, 328
Hpy188I TCNGA 4 cut(s) 250, 409, 456, 468
Hpy188III TCNNGA 3 cut(s) 193, 319, 584
Hpy8I GTNNAC 3 cut(s) 181, 297, 328
Hpy99I CGWCG 7 cut(s) 79, 82, 182, 185, 188, 254, 329
HpyAV CCTTC 2 cut(s) 358, 409
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4IV ACGT 5 cut(s) 77, 183, 233, 274, 332
HpyCH4V TGCA 1 cut(s) 168
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 260, 571
HpyF3I CTNAG 1 cut(s) 199
HpySE526I ACGT 5 cut(s) 77, 183, 233, 274, 332
Hsp92I GRCGYC 6 cut(s) 77, 177, 183, 189, 252, 302
Hsp92II CATG 1 cut(s) 295
HspAI GCGC 1 cut(s) 302
KasI GGCGCC 1 cut(s) 301
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 3 cut(s) 321, 552, 580
LmnI GCTCC 2 cut(s) 273, 398
Lsp1109I GCAGC 2 cut(s) 180, 549
MaeII ACGT 5 cut(s) 77, 183, 233, 274, 332
MalI GATC 3 cut(s) 323, 554, 582
MboI GATC 3 cut(s) 321, 552, 580
MboII GAAGA 3 cut(s) 214, 508, 562
MflI RGATCY 1 cut(s) 552
MhlI GDGCHC 2 cut(s) 360, 425
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 6 cut(s) 15, 30, 393, 457, 477, 588
MluNI TGGCCA 1 cut(s) 230
Mly113I GGCGCC 1 cut(s) 302
MmeI TCCRAC 2 cut(s) 273, 295
MnlI CCTC 8 cut(s) 75, 97, 108, 137, 140, 208, 266, 288
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
MseI TTAA 1 cut(s) 33
Msp20I TGGCCA 1 cut(s) 230
MspI CCGG 2 cut(s) 174, 389
MwoI GCNNNNNNNGC 3 cut(s) 174, 260, 571
NarI GGCGCC 1 cut(s) 302
NdeII GATC 3 cut(s) 321, 552, 580
NlaIII CATG 1 cut(s) 295
NlaIV GGNNCC 1 cut(s) 303
NspI RCATGY 1 cut(s) 295
PcsI WCGNNNNNNNCGW 3 cut(s) 86, 186, 324
PfeI GAWTC 2 cut(s) 404, 463
PkrI GCNGC 3 cut(s) 136, 170, 564
PluTI GGCGCC 1 cut(s) 305
PmaCI CACGTG 2 cut(s) 234, 333
PmlI CACGTG 2 cut(s) 234, 333
Ppu21I YACGTR 2 cut(s) 234, 333
PpuMI RGGWCCY 1 cut(s) 84
Psp124BI GAGCTC 1 cut(s) 425
Psp5II RGGWCCY 1 cut(s) 84
PspCI CACGTG 2 cut(s) 234, 333
PspFI CCCAGC 2 cut(s) 52, 264
PspN4I GGNNCC 1 cut(s) 303
PspPI GGNCC 1 cut(s) 84
PspPPI RGGWCCY 1 cut(s) 84
PstI CTGCAG 1 cut(s) 170
PsuI RGATCY 1 cut(s) 552
SacI GAGCTC 1 cut(s) 425
SalI GTCGAC 2 cut(s) 179, 326
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 3 cut(s) 135, 169, 563
Sau3AI GATC 3 cut(s) 321, 552, 580
Sau96I GGNCC 1 cut(s) 84
SduI GDGCHC 2 cut(s) 360, 425
SfcI CTRYAG 1 cut(s) 166
SfoI GGCGCC 1 cut(s) 303
SgrAI CRCCGGYG 1 cut(s) 173
SgrDI CGTCGACG 1 cut(s) 179
SinI GGWCC 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 556
SmoI CTYRAG 1 cut(s) 556
Sse9I AATT 6 cut(s) 15, 30, 393, 457, 477, 588
SsiI CCGC 2 cut(s) 135, 312
SspDI GGCGCC 1 cut(s) 301
SstI GAGCTC 1 cut(s) 425
StyI CCWWGG 1 cut(s) 383
TaaI ACNGT 1 cut(s) 117
TaiI ACGT 5 cut(s) 80, 186, 236, 277, 335
TaqI TCGA 8 cut(s) 74, 80, 89, 180, 194, 318, 327, 377
TasI AATT 6 cut(s) 15, 30, 393, 457, 477, 588
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 2 cut(s) 404, 463
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TscAI CASTG 1 cut(s) 63
TseI GCWGC 2 cut(s) 168, 562
TspDTI ATGAA 3 cut(s) 17, 509, 563
TspRI CASTG 1 cut(s) 63
VpaK11BI GGWCC 1 cut(s) 84
XapI RAATTY 2 cut(s) 15, 457
XceI RCATGY 1 cut(s) 295
XcmI CCANNNNNNNNNTGG 1 cut(s) 439
XmiI GTMKAC 2 cut(s) 180, 327
ZraI GACGTC 2 cut(s) 78, 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.