Rw4G012310

DNA-damage-repair toleration protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016457
Physical Location & Seq
Reverse (-)
28991039 .. 28991641
603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012310.1

Sequence Viewer

Length: 603 bp
ATGAACTCAACCAAAATTCACATCCCAGAAATTGAAAATCAAAATCAAATCCTAGCAGTGGCTCATTCAACATCGACGTCGAGGACCTCGACACTTCCTCCTACTTCTCCATCGCCGTTGCGTCTCCTCCTCGCCACCAATACCAAAACTCGCGGACTTATTGCCTACAGCACTGGCATCGGTGTCGTGACCTTAGCTAACAAGTTCCCCAAAGTCTTCACGGCCACGTGTCTCTCCCCGTCTGACGCCCTCAACGCCCAGCTCCAAAGTTCTCCTGTCTCCGACATGTCCACGGCGCCGGAATCCGCCATTAAGATCATCGACACATGGCTCAACACTCCCTTCAAAGTGCCATGTCCTGCGTCGAAGTTGAAACCTTGGCCGGAAATTAGAGCTGATTCTGATTTGAAGGAGTGCTCTTGTTCCATCTGTTGTTTGGTGAAGAATCAGAATTTGAATCCGATTGATATAATTTCGGGTCATTCAATGAAGATACTGAGTTCTTTATCAAGTGGGATAGGAAATGGGATATGTTATTTGATGAAGATCTCAAGGCTGCAAACAAGGCTATTGATCAAGAAATTGGAGAATGGGGGTAAGTGA

Protein Analysis

200

Amino Acids

21.53

Weight (kDa)

9.35

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LacAB_rpiB PF02502 42 - 116 2.5e-07 Ribose/Galactose Isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 80
AccB1I GGYRCC 1 cut(s) 295
AccII CGCG 1 cut(s) 153
AciI CCGC 2 cut(s) 153, 306
AcoI YGGCCR 2 cut(s) 222, 380
AcsI RAATTY 2 cut(s) 15, 451
AcvI CACGTG 1 cut(s) 228
AcyI GRCGYC 3 cut(s) 77, 246, 296
AfiI CCNNNNNNNGG 1 cut(s) 58
AflIII ACRYGT 2 cut(s) 227, 285
AgsI TTSAA 7 cut(s) 35, 69, 346, 373, 409, 457, 486
AluBI AGCT 3 cut(s) 197, 262, 395
AluI AGCT 3 cut(s) 197, 262, 395
Alw21I GWGCWC 1 cut(s) 419
Alw26I GTCTC 3 cut(s) 128, 236, 283
AoxI GGCC 2 cut(s) 222, 380
ApeKI GCWGC 1 cut(s) 556
ApoI RAATTY 2 cut(s) 15, 451
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 451
AvaII GGWCC 1 cut(s) 84
BanI GGYRCC 1 cut(s) 295
BbrPI CACGTG 1 cut(s) 228
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 419
BbvI GCAGC 1 cut(s) 543
BccI CCATC 2 cut(s) 118, 434
BceAI ACGGC 3 cut(s) 100, 237, 309
BcgI CGANNNNNNTGC 4 cut(s) 160, 166, 194, 200
BclI TGATCA 1 cut(s) 573
BcoDI GTCTC 3 cut(s) 128, 236, 283
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 1 cut(s) 299
BglII AGATCT 1 cut(s) 546
BisI GCNGC 1 cut(s) 557
BlsI GCNGC 1 cut(s) 558
Bme18I GGWCC 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 297
BmsI GCATC 1 cut(s) 186
BpiI GAAGAC 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 193
BpuEI CTTGAG 1 cut(s) 535
BsaAI YACGTR 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 545
BsaHI GRCGYC 3 cut(s) 77, 246, 296
BsaJI CCNNGG 2 cut(s) 291, 377
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 178
Bse8I GATNNNNATC 1 cut(s) 545
BseDI CCNNGG 2 cut(s) 291, 377
BseGI GGATG 1 cut(s) 21
BseJI GATNNNNATC 1 cut(s) 545
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMII CTCAG 1 cut(s) 488
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 2 cut(s) 116, 119
BseXI GCAGC 1 cut(s) 543
BseYI CCCAGC 1 cut(s) 258
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 2 cut(s) 224, 382
BshNI GGYRCC 1 cut(s) 295
BsiHKAI GWGCWC 1 cut(s) 419
BsiSI CCGG 2 cut(s) 299, 383
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 3 cut(s) 128, 236, 283
BsmBI CGTCTC 1 cut(s) 128
BsnI GGCC 2 cut(s) 224, 382
Bsp1286I GDGCHC 1 cut(s) 419
Bsp143I GATC 3 cut(s) 315, 546, 573
BspACI CCGC 2 cut(s) 153, 306
BspANI GGCC 2 cut(s) 224, 382
BspCNI CTCAG 1 cut(s) 489
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 297
BspT107I GGYRCC 1 cut(s) 295
BsrI ACTGG 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 291, 377
BssMI GATC 3 cut(s) 315, 546, 573
BssNI GRCGYC 3 cut(s) 77, 246, 296
BssT1I CCWWGG 1 cut(s) 377
BstACI GRCGYC 3 cut(s) 77, 246, 296
BstBAI YACGTR 1 cut(s) 228
BstDEI CTNAG 2 cut(s) 193, 497
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 1 cut(s) 21
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 3 cut(s) 318, 549, 576
BstMAI GTCTC 3 cut(s) 128, 236, 283
BstMBI GATC 3 cut(s) 315, 546, 573
BstMWI GCNNNNNNNGC 2 cut(s) 254, 565
BstNSI RCATGY 1 cut(s) 289
BstSFI CTRYAG 1 cut(s) 166
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 1 cut(s) 543
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 546
BstYI RGATCY 1 cut(s) 546
BsuRI GGCC 2 cut(s) 224, 382
BtgI CCRYGG 1 cut(s) 291
BtgZI GCGATG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 21
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 2 cut(s) 63, 171
CfoI GCGC 1 cut(s) 298
Cfr13I GGNCC 1 cut(s) 84
CseI GACGC 3 cut(s) 110, 254, 351
CviAII CATG 3 cut(s) 286, 327, 354
CviJI RGCY 9 cut(s) 62, 197, 224, 262, 331, 382, 395, 556, 568
CviKI_1 RGCY 9 cut(s) 62, 197, 224, 262, 331, 382, 395, 556, 568
DdeI CTNAG 2 cut(s) 193, 497
DinI GGCGCC 1 cut(s) 297
DpnI GATC 3 cut(s) 317, 548, 575
DpnII GATC 3 cut(s) 315, 546, 573
EaeI YGGCCR 2 cut(s) 222, 380
EciI GGCGGA 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 377
Eco47I GGWCC 1 cut(s) 84
Eco72I CACGTG 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 377
EgeI GGCGCC 1 cut(s) 297
EheI GGCGCC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 377
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 3 cut(s) 289, 330, 357
FaiI YATR 5 cut(s) 287, 328, 355, 470, 532
FatI CATG 3 cut(s) 285, 326, 353
FbaI TGATCA 1 cut(s) 573
Fnu4HI GCNGC 1 cut(s) 557
FokI GGATG 1 cut(s) 8
Fsp4HI GCNGC 1 cut(s) 557
FspBI CTAG 1 cut(s) 53
GlaI GCGC 1 cut(s) 297
GluI GCNGC 1 cut(s) 557
GsaI CCCAGC 1 cut(s) 262
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 2 cut(s) 224, 382
HapII CCGG 2 cut(s) 299, 383
HgaI GACGC 3 cut(s) 110, 254, 351
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 3 cut(s) 77, 246, 296
Hin1II CATG 3 cut(s) 289, 330, 357
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HinfI GANTC 4 cut(s) 302, 398, 445, 457
HpaII CCGG 2 cut(s) 299, 383
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 1 cut(s) 291
Hpy188I TCNGA 5 cut(s) 244, 283, 403, 450, 462
Hpy188III TCNNGA 2 cut(s) 187, 577
Hpy8I GTNNAC 1 cut(s) 291
Hpy99I CGWCG 3 cut(s) 79, 82, 367
HpyAV CCTTC 2 cut(s) 352, 403
HpyCH4IV ACGT 2 cut(s) 77, 227
HpyCH4V TGCA 1 cut(s) 559
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 565
HpyF3I CTNAG 2 cut(s) 193, 497
HpySE526I ACGT 2 cut(s) 77, 227
Hsp92I GRCGYC 3 cut(s) 77, 246, 296
Hsp92II CATG 3 cut(s) 289, 330, 357
HspAI GCGC 1 cut(s) 296
KasI GGCGCC 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 573
Kzo9I GATC 3 cut(s) 315, 546, 573
LmnI GCTCC 1 cut(s) 267
LpnPI CCDG 7 cut(s) 39, 159, 272, 288, 312, 372, 396
Lsp1109I GCAGC 1 cut(s) 543
LweI GCATC 1 cut(s) 186
MaeI CTAG 1 cut(s) 53
MaeII ACGT 2 cut(s) 77, 227
MaeIII GTNAC 1 cut(s) 187
MalI GATC 3 cut(s) 317, 548, 575
MboI GATC 3 cut(s) 315, 546, 573
MboII GAAGA 4 cut(s) 208, 454, 502, 556
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 1 cut(s) 419
MluCI AATT 6 cut(s) 15, 30, 387, 451, 471, 581
Mly113I GGCGCC 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 306
MnlI CCTC 6 cut(s) 75, 97, 108, 137, 140, 260
MseI TTAA 1 cut(s) 312
MspI CCGG 2 cut(s) 299, 383
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 2 cut(s) 254, 565
NarI GGCGCC 1 cut(s) 296
NdeII GATC 3 cut(s) 315, 546, 573
NlaIII CATG 3 cut(s) 289, 330, 357
NlaIV GGNNCC 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 187
NspI RCATGY 1 cut(s) 289
PciI ACATGT 1 cut(s) 285
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PfeI GAWTC 4 cut(s) 302, 398, 445, 457
PkrI GCNGC 1 cut(s) 558
PluTI GGCGCC 1 cut(s) 299
PmaCI CACGTG 1 cut(s) 228
PmlI CACGTG 1 cut(s) 228
Ppu21I YACGTR 1 cut(s) 228
PpuMI RGGWCCY 1 cut(s) 84
PscI ACATGT 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 84
PspCI CACGTG 1 cut(s) 228
PspFI CCCAGC 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 1 cut(s) 84
PspPPI RGGWCCY 1 cut(s) 84
PsuI RGATCY 1 cut(s) 546
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 1 cut(s) 557
Sau3AI GATC 3 cut(s) 315, 546, 573
Sau96I GGNCC 1 cut(s) 84
SduI GDGCHC 1 cut(s) 419
SetI ASST 8 cut(s) 80, 89, 194, 199, 230, 264, 379, 397
SfaNI GCATC 1 cut(s) 186
SfcI CTRYAG 1 cut(s) 166
SfoI GGCGCC 1 cut(s) 297
SinI GGWCC 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
Sse9I AATT 6 cut(s) 15, 30, 387, 451, 471, 581
SsiI CCGC 2 cut(s) 153, 306
SspDI GGCGCC 1 cut(s) 295
SspMI CTAG 1 cut(s) 53
StyI CCWWGG 1 cut(s) 377
TaiI ACGT 2 cut(s) 80, 230
TaqI TCGA 5 cut(s) 74, 80, 89, 321, 365
TasI AATT 6 cut(s) 15, 30, 387, 451, 471, 581
TfiI GAWTC 4 cut(s) 302, 398, 445, 457
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 2 cut(s) 63, 178
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 1 cut(s) 556
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 3 cut(s) 17, 503, 557
TspRI CASTG 2 cut(s) 63, 178
VpaK11BI GGWCC 1 cut(s) 84
XapI RAATTY 2 cut(s) 15, 451
XceI RCATGY 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XspI CTAG 1 cut(s) 53
ZraI GACGTC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.