RLG00000004669

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011721
Physical Location & Seq
Forward (+)
61303618 .. 61309259
5642 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004669

Sequence Viewer

Length: 1182 bp
ATGGGCTGCTTCCAGGGAGGTCCCGGTCGCCTCCATATCCTTATTTGTCTCGATGATTGTGCCTTGCGGCAAATTGTTTTCATTATCAATGTGAGGGAAGGATTGACAGATTTTCAAGGGTGGGACTGGGCTGTTAGTATTGGCGCTGCAAAGCAGCAACCAGATCTTTTTTGGGTGGATTGTAGTGATCTTTTCAATGCCGGAGTTGATGGTGTGGCCGACAATGGTGGCCTTGATGCAAGTGGCGCCGGAGTAGTGAGCTCTTTGATCCCTCTTTCCATTTGCTATCTTCTTGATCTTGTGGGATCAATTCAAACTAACAAAGTGGATCATAGAAAGCATGGGAAGAGGGCCTTTCCTCGTGATGGATCGGAGGAGAAAGAGGAAGAGGGTGGTCAAGATCAAGTAGGCTTGTTCCCAGCGGCGCCGTTTTGCTCATCATCATCCTCAGCTCGATCTCAACAAGAGATGTCTGCCATGGTCTCAGCTCTATCACAAGTTATTAGTGGAAACAGTAGTACTGATACTAACCAAAACCCTGCACTTCAACTTCCCCAACAGTCCGGAGCAGCCGTATCGGAACATGATCCATCCCATCAACAAGCGCCAGATCAAGGGGCTGTGAGGAGGAGACACTATAGAGGAGTGAGGCAAAGACCATGGGGGAAGTGGGCGGCAGAGATTCGTGATCCAAAGAAGGCAGCCCGAGTGTGGCTTGGAACTTTTGAAACGGCGGAGGCTGCTGCACTTGCTTACGACGAAGCAGCTCTCAAATTCAAAGGAAGCAAGGCTAAGCTTAACTTCCCGGAAAGAGTTCAAGGGACGAGTGAATTAGGCGGTTACCTCACAACTAGCACAGTTGCTCATCAGCCTCTCATTTCTGATTATCATCATCAGCAACTGGTATACCCGAATAATATCCCTAATACTGGTCAATATTATCCACAATTTTATGGGAACTTAAACCATGGTCAGACAAATCAAAGATTTTATTACCAGCCTACTACGTCTTCATCTCCACCTTTTATACCAATACCTCAAGCACAAGAAGAAGATCAACAACAAAAGCCGGCCTTAAGCTTTCCTATGCCGGCGTTTGGTTCATCTTCTTCCAGTTTGGATCCTGAATATCCACAGAACAATACTACAAGAAATTTTGACAATAGTCATTCGAGAGGATAG

Protein Analysis

394

Amino Acids

42.96

Weight (kDa)

5.77

Isoelectric Point (pI)

49.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 212 - 261 2.2e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011721)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43160
fragaria_vesca FvH4_5g09511 FvH4_5g09511
malus_domestica MD06G1130400.v1.1 MD14G1147200.v1.1
prunus_persica Prupe.5G141300_v2.0.a1
pyrus_communis pycom06g12220 pycom14g12180 pycom14g12190
rosa_chinensis RchiOBHm_Chr7g0188691
rosa_laevigata RLG00000004669
rosa_multiflora Rmu_sc0001576.1_g000029
rosa_roxburghii Rroxscaffold_3G00266130
rosa_rugosa Rorug06G0492300
rosa_samantha Rh7AG097200 Rh7BG099000 Rh7CG100800 Rh7DG099500
rosa_wichuraiana Rw7G008410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 245, 424
AccI GTMKAC 1 cut(s) 906
AccIII TCCGGA 1 cut(s) 563
AciI CCGC 5 cut(s) 67, 422, 674, 734, 837
AclWI GGATC 8 cut(s) 262, 313, 336, 376, 581, 683, 1115, 1128
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 2 cut(s) 773, 1153
AcyI GRCGYC 2 cut(s) 246, 425
AfaI GTAC 1 cut(s) 520
AfiI CCNNNNNNNGG 5 cut(s) 365, 614, 711, 929, 1097
AflII CTTAAG 1 cut(s) 1075
AgsI TTSAA 7 cut(s) 116, 196, 314, 548, 728, 778, 818
AjnI CCWGG 1 cut(s) 12
AluBI AGCT 6 cut(s) 261, 452, 488, 767, 796, 1080
AluI AGCT 6 cut(s) 261, 452, 488, 767, 796, 1080
Alw21I GWGCWC 1 cut(s) 263
Alw26I GTCTC 3 cut(s) 53, 487, 625
AlwI GGATC 8 cut(s) 262, 313, 336, 376, 581, 683, 1115, 1128
AlwNI CAGNNNCTG 1 cut(s) 901
Ama87I CYCGRG 1 cut(s) 705
Aor13HI TCCGGA 1 cut(s) 563
AoxI GGCC 4 cut(s) 216, 229, 351, 1071
ApeKI GCWGC 8 cut(s) 6, 146, 154, 569, 701, 740, 743, 764
ApoI RAATTY 2 cut(s) 773, 1153
Asp700I GAANNNNTTC 1 cut(s) 813
AspLEI GCGC 4 cut(s) 146, 248, 427, 607
AspS9I GGNCC 2 cut(s) 20, 351
AsuC2I CCSGG 2 cut(s) 24, 806
AvaI CYCGRG 1 cut(s) 705
AvaII GGWCC 1 cut(s) 20
BamHI GGATCC 1 cut(s) 1120
BanI GGYRCC 2 cut(s) 245, 424
BanII GRGCYC 1 cut(s) 263
BauI CACGAG 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 1002
Bbv12I GWGCWC 1 cut(s) 263
BbvCI CCTCAGC 1 cut(s) 448
BbvI GCAGC 7 cut(s) 133, 166, 581, 713, 727, 730, 776
BccI CCATC 4 cut(s) 203, 359, 598, 603
BceAI ACGGC 3 cut(s) 412, 557, 747
BcgI CGANNNNNNTGC 4 cut(s) 41, 75, 558, 592
BciT130I CCWGG 1 cut(s) 14
BcnI CCSGG 2 cut(s) 24, 806
BcoDI GTCTC 3 cut(s) 53, 487, 625
BfaI CTAG 1 cut(s) 852
BfmI CTRYAG 1 cut(s) 637
BfoI RGCGCY 4 cut(s) 147, 249, 428, 608
BfrI CTTAAG 1 cut(s) 1075
BglII AGATCT 1 cut(s) 163
BlpI GCTNAGC 1 cut(s) 792
BmcAI AGTACT 1 cut(s) 520
Bme1390I CCNGG 3 cut(s) 14, 24, 806
Bme18I GGWCC 1 cut(s) 20
BmeT110I CYCGRG 1 cut(s) 705
BmgT120I GGNCC 2 cut(s) 20, 351
BmiI GGNNCC 4 cut(s) 22, 247, 426, 1122
BmrFI CCNGG 3 cut(s) 14, 24, 806
BmrI ACTGGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 226
BmuI ACTGGG 1 cut(s) 136
BoxI GACNNNNGTC 1 cut(s) 1164
BpiI GAAGAC 1 cut(s) 1002
Bpu10I CCTNAGC 1 cut(s) 448
Bpu1102I GCTNAGC 1 cut(s) 792
BpuEI CTTGAG 1 cut(s) 1023
BpuMI CCSGG 2 cut(s) 24, 806
BsaBI GATNNNNATC 1 cut(s) 888
BsaHI GRCGYC 2 cut(s) 246, 425
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 4 cut(s) 13, 477, 659, 967
BsaWI WCCGGW 1 cut(s) 563
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 5 cut(s) 365, 614, 711, 929, 1097
Bse118I RCCGGY 2 cut(s) 1069, 1090
Bse1I ACTGG 4 cut(s) 131, 906, 934, 1113
Bse8I GATNNNNATC 1 cut(s) 888
BseAI TCCGGA 1 cut(s) 563
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 4 cut(s) 13, 477, 659, 967
BseGI GGATG 2 cut(s) 443, 590
BseJI GATNNNNATC 1 cut(s) 888
BseLI CCNNNNNNNGG 5 cut(s) 365, 614, 711, 929, 1097
BseMII CTCAG 2 cut(s) 462, 498
BseNI ACTGG 4 cut(s) 131, 906, 934, 1113
BseRI GAGGAG 4 cut(s) 389, 640, 643, 657
BseXI GCAGC 7 cut(s) 133, 166, 581, 713, 727, 730, 776
BseYI CCCAGC 1 cut(s) 418
BsgI GTGCAG 2 cut(s) 525, 729
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 4 cut(s) 218, 231, 353, 1073
BshNI GGYRCC 2 cut(s) 245, 424
BsiEI CGRYCG 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 263
BsiHKCI CYCGRG 1 cut(s) 705
BsiSI CCGG 7 cut(s) 24, 201, 249, 564, 806, 1070, 1091
BslFI GGGAC 3 cut(s) 6, 137, 835
BslI CCNNNNNNNGG 5 cut(s) 365, 614, 711, 929, 1097
BsmAI GTCTC 3 cut(s) 53, 487, 625
BsmFI GGGAC 3 cut(s) 6, 137, 835
BsnI GGCC 4 cut(s) 218, 231, 353, 1073
Bso31I GGTCTC 1 cut(s) 487
BsoBI CYCGRG 1 cut(s) 705
Bsp1286I GDGCHC 1 cut(s) 263
Bsp13I TCCGGA 1 cut(s) 563
Bsp1720I GCTNAGC 1 cut(s) 792
Bsp19I CCATGG 3 cut(s) 477, 659, 967
BspACI CCGC 5 cut(s) 67, 422, 674, 734, 837
BspANI GGCC 4 cut(s) 218, 231, 353, 1073
BspCNI CTCAG 2 cut(s) 461, 497
BspEI TCCGGA 1 cut(s) 563
BspLI GGNNCC 4 cut(s) 22, 247, 426, 1122
BspPI GGATC 8 cut(s) 262, 313, 336, 376, 581, 683, 1115, 1128
BspT107I GGYRCC 2 cut(s) 245, 424
BspTI CTTAAG 1 cut(s) 1075
BspTNI GGTCTC 1 cut(s) 487
BsrFI RCCGGY 2 cut(s) 1069, 1090
BsrI ACTGG 4 cut(s) 131, 906, 934, 1113
BssAI RCCGGY 2 cut(s) 1069, 1090
BssECI CCNNGG 4 cut(s) 13, 477, 659, 967
BssNAI GTATAC 1 cut(s) 907
BssNI GRCGYC 2 cut(s) 246, 425
BssSI CACGAG 1 cut(s) 360
BssT1I CCWWGG 3 cut(s) 477, 659, 967
Bst1107I GTATAC 1 cut(s) 907
Bst2BI CACGAG 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 3 cut(s) 515, 561, 859
Bst6I CTCTTC 2 cut(s) 341, 381
BstACI GRCGYC 2 cut(s) 246, 425
BstAFI CTTAAG 1 cut(s) 1075
BstC8I GCNNGC 2 cut(s) 1071, 1092
BstDEI CTNAG 3 cut(s) 448, 484, 792
BstDSI CCRYGG 3 cut(s) 477, 659, 967
BstEII GGTNACC 1 cut(s) 839
BstF5I GGATG 2 cut(s) 443, 590
BstH2I RGCGCY 4 cut(s) 147, 249, 428, 608
BstHHI GCGC 4 cut(s) 146, 248, 427, 607
BstMAI GTCTC 3 cut(s) 53, 487, 625
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 3 cut(s) 245, 740, 749
BstNI CCWGG 1 cut(s) 14
BstPAI GACNNNNGTC 1 cut(s) 1164
BstPI GGTNACC 1 cut(s) 839
BstSCI CCNGG 3 cut(s) 12, 22, 804
BstSFI CTRYAG 1 cut(s) 637
BstV1I GCAGC 7 cut(s) 133, 166, 581, 713, 727, 730, 776
BstV2I GAAGAC 1 cut(s) 1002
BstX2I RGATCY 2 cut(s) 163, 1120
BstYI RGATCY 2 cut(s) 163, 1120
BstZ17I GTATAC 1 cut(s) 907
BsuRI GGCC 4 cut(s) 218, 231, 353, 1073
BtgI CCRYGG 3 cut(s) 477, 659, 967
BtsCI GGATG 2 cut(s) 443, 590
Cac8I GCNNGC 2 cut(s) 1071, 1092
CaiI CAGNNNCTG 1 cut(s) 901
CfoI GCGC 4 cut(s) 146, 248, 427, 607
Cfr10I RCCGGY 2 cut(s) 1069, 1090
Cfr13I GGNCC 2 cut(s) 20, 351
Csp6I GTAC 1 cut(s) 519
CviAII CATG 5 cut(s) 341, 478, 584, 660, 968
CviQI GTAC 1 cut(s) 519
DdeI CTNAG 3 cut(s) 448, 484, 792
DinI GGCGCC 2 cut(s) 247, 426
EaeI YGGCCR 1 cut(s) 216
Eam1104I CTCTTC 2 cut(s) 341, 381
EarI CTCTTC 2 cut(s) 341, 381
EciI GGCGGA 1 cut(s) 749
Ecl136II GAGCTC 1 cut(s) 261
Eco130I CCWWGG 3 cut(s) 477, 659, 967
Eco24I GRGCYC 1 cut(s) 263
Eco31I GGTCTC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 20
Eco53kI GAGCTC 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 705
Eco91I GGTNACC 1 cut(s) 839
EcoICRI GAGCTC 1 cut(s) 261
EcoO109I RGGNCCY 2 cut(s) 20, 351
EcoO65I GGTNACC 1 cut(s) 839
EcoRII CCWGG 1 cut(s) 12
EcoT14I CCWWGG 3 cut(s) 477, 659, 967
EcoT38I GRGCYC 1 cut(s) 263
EgeI GGCGCC 2 cut(s) 247, 426
EheI GGCGCC 2 cut(s) 247, 426
ErhI CCWWGG 3 cut(s) 477, 659, 967
FaeI CATG 5 cut(s) 344, 481, 587, 663, 971
FalI AAGNNNNNCTT 6 cut(s) 338, 370, 785, 817, 1058, 1090
FaqI GGGAC 3 cut(s) 6, 137, 835
FatI CATG 5 cut(s) 340, 477, 583, 659, 967
FblI GTMKAC 1 cut(s) 906
FokI GGATG 2 cut(s) 430, 577
FriOI GRGCYC 1 cut(s) 263
FspBI CTAG 1 cut(s) 852
GlaI GCGC 4 cut(s) 145, 247, 426, 606
GsaI CCCAGC 1 cut(s) 422
HaeII RGCGCY 4 cut(s) 147, 249, 428, 608
HaeIII GGCC 4 cut(s) 218, 231, 353, 1073
HapII CCGG 7 cut(s) 24, 201, 249, 564, 806, 1070, 1091
HhaI GCGC 4 cut(s) 146, 248, 427, 607
Hin1I GRCGYC 2 cut(s) 246, 425
Hin1II CATG 5 cut(s) 344, 481, 587, 663, 971
Hin6I GCGC 4 cut(s) 144, 246, 425, 605
HinP1I GCGC 4 cut(s) 144, 246, 425, 605
HindIII AAGCTT 2 cut(s) 794, 1078
HinfI GANTC 1 cut(s) 682
HpaII CCGG 7 cut(s) 24, 201, 249, 564, 806, 1070, 1091
Hpy166II GTNNAC 1 cut(s) 907
Hpy188I TCNGA 4 cut(s) 373, 580, 883, 975
Hpy188III TCNNGA 8 cut(s) 50, 293, 362, 398, 564, 686, 1124, 1173
Hpy8I GTNNAC 1 cut(s) 907
Hpy99I CGWCG 1 cut(s) 761
HpyAV CCTTC 2 cut(s) 92, 691
HpyCH4III ACNGT 3 cut(s) 515, 561, 859
HpyCH4IV ACGT 1 cut(s) 1007
HpyCH4V TGCA 4 cut(s) 149, 239, 542, 746
HpyF10VI GCNNNNNNNGC 3 cut(s) 245, 740, 749
HpyF3I CTNAG 3 cut(s) 448, 484, 792
HpySE526I ACGT 1 cut(s) 1007
Hsp92I GRCGYC 2 cut(s) 246, 425
Hsp92II CATG 5 cut(s) 344, 481, 587, 663, 971
HspAI GCGC 4 cut(s) 144, 246, 425, 605
KasI GGCGCC 2 cut(s) 245, 424
Kpn2I TCCGGA 1 cut(s) 563
KroI GCCGGC 2 cut(s) 1069, 1090
KroNI GCCGGC 2 cut(s) 1071, 1092
LmnI GCTCC 1 cut(s) 566
Lsp1109I GCAGC 7 cut(s) 133, 166, 581, 713, 727, 730, 776
LweI GCATC 1 cut(s) 226
MaeI CTAG 1 cut(s) 852
MaeII ACGT 1 cut(s) 1007
MaeIII GTNAC 1 cut(s) 839
MboII GAAGA 8 cut(s) 281, 358, 398, 1002, 1061, 1064, 1098, 1101
MflI RGATCY 2 cut(s) 163, 1120
MhlI GDGCHC 1 cut(s) 263
MluCI AATT 6 cut(s) 72, 309, 773, 830, 947, 1153
Mly113I GGCGCC 2 cut(s) 246, 425
MroI TCCGGA 1 cut(s) 563
MroNI GCCGGC 2 cut(s) 1069, 1090
MroXI GAANNNNTTC 1 cut(s) 813
MseI TTAA 3 cut(s) 798, 962, 1076
MspA1I CMGCKG 1 cut(s) 422
MspCI CTTAAG 1 cut(s) 1075
MspI CCGG 7 cut(s) 24, 201, 249, 564, 806, 1070, 1091
MspR9I CCNGG 3 cut(s) 14, 24, 806
MvaI CCWGG 1 cut(s) 14
MwoI GCNNNNNNNGC 3 cut(s) 245, 740, 749
NaeI GCCGGC 2 cut(s) 1071, 1092
NarI GGCGCC 2 cut(s) 246, 425
NciI CCSGG 2 cut(s) 24, 806
NcoI CCATGG 3 cut(s) 477, 659, 967
NgoMIV GCCGGC 2 cut(s) 1069, 1090
NlaIII CATG 5 cut(s) 344, 481, 587, 663, 971
NlaIV GGNNCC 4 cut(s) 22, 247, 426, 1122
PdiI GCCGGC 2 cut(s) 1071, 1092
PdmI GAANNNNTTC 1 cut(s) 813
PfeI GAWTC 1 cut(s) 682
PfoI TCCNGGA 1 cut(s) 804
PluTI GGCGCC 2 cut(s) 249, 428
PpuMI RGGWCCY 1 cut(s) 20
PshAI GACNNNNGTC 1 cut(s) 1164
Psp124BI GAGCTC 1 cut(s) 263
Psp5II RGGWCCY 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 12
PspEI GGTNACC 1 cut(s) 839
PspFI CCCAGC 1 cut(s) 418
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 4 cut(s) 22, 247, 426, 1122
PspPI GGNCC 2 cut(s) 20, 351
PspPPI RGGWCCY 1 cut(s) 20
PstNI CAGNNNCTG 1 cut(s) 901
PsuI RGATCY 2 cut(s) 163, 1120
RsaI GTAC 1 cut(s) 520
RsaNI GTAC 1 cut(s) 519
SacI GAGCTC 1 cut(s) 263
SaqAI TTAA 3 cut(s) 798, 962, 1076
Sau96I GGNCC 2 cut(s) 20, 351
ScaI AGTACT 1 cut(s) 520
ScrFI CCNGG 3 cut(s) 14, 24, 806
SduI GDGCHC 1 cut(s) 263
SfaNI GCATC 1 cut(s) 226
SfcI CTRYAG 1 cut(s) 637
SfoI GGCGCC 2 cut(s) 247, 426
SinI GGWCC 1 cut(s) 20
SmlI CTYRAG 2 cut(s) 1038, 1075
SmoI CTYRAG 2 cut(s) 1038, 1075
Sse9I AATT 6 cut(s) 72, 309, 773, 830, 947, 1153
SsiI CCGC 5 cut(s) 67, 422, 674, 734, 837
SspDI GGCGCC 2 cut(s) 245, 424
SspI AATATT 1 cut(s) 938
SspMI CTAG 1 cut(s) 852
SstI GAGCTC 1 cut(s) 263
StyD4I CCNGG 3 cut(s) 12, 22, 804
StyI CCWWGG 3 cut(s) 477, 659, 967
TaaI ACNGT 3 cut(s) 515, 561, 859
TaiI ACGT 1 cut(s) 1010
TaqI TCGA 3 cut(s) 51, 454, 1172
TasI AATT 6 cut(s) 72, 309, 773, 830, 947, 1153
TatI WGTACW 1 cut(s) 518
TauI GCSGC 3 cut(s) 70, 425, 677
TfiI GAWTC 1 cut(s) 682
Tru1I TTAA 3 cut(s) 798, 962, 1076
Tru9I TTAA 3 cut(s) 798, 962, 1076
TseI GCWGC 8 cut(s) 6, 146, 154, 569, 701, 740, 743, 764
TspDTI ATGAA 3 cut(s) 70, 1002, 1092
Vha464I CTTAAG 1 cut(s) 1075
VpaK11BI GGWCC 1 cut(s) 20
XapI RAATTY 2 cut(s) 773, 1153
XcmI CCANNNNNNNNNTGG 2 cut(s) 168, 666
XmiI GTMKAC 1 cut(s) 906
XmnI GAANNNNTTC 1 cut(s) 813
XspI CTAG 1 cut(s) 852
ZrmI AGTACT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.