Rh7DG099500

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0011721
Physical Location & Seq
Reverse (-)
8261921 .. 8272315
10395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG099500.1

Sequence Viewer

Length: 1137 bp
ATGGGAAACTGCTGCTCCGACGACGCCGGCGGCCAGACGGCCGTCGGCGGCACCGCCGCTTCTCACGGCAATCCCAACGACGCTCACAACGACGCCGTCGATCACCTCCTCAAGACCCGCGGCTACCACGGCCTCTTCTCTCAGATCGAGTTATCCTTGGCTGCTAAAGACTTGCGTGATCGGGACGTGCTCTCCAAGCTCAAAAATCACTGGCTGACCTGGAAAAGCTTCATACTAGTGGACAAGGAGAAAATTTATTTATACCTGCTGGGCATGATCACCACAACAAGAAAGCATGGGAAGAGGGCCTTCCCTCGTGATGGATCGGAGGAGAGAGAGGAAGAGGGTGGTCAGGATCAAGTAGGGTTGCTCCCAGCGGCGCCGTTTTGCTCATCATCATCCTCAGCTCGATCTGAACAAGAGATGTCTGCCATGGTCTCAGCTCTATCACAAGTTATTAGTGGAAACAGTAGTACTGATACTAACCAAAACCCTGCACTTCAACTTCCCCAACAGTCCGGCGCCGCCGTATTGGAACATGATCAATCCCATCAACAAGCGCCAGATCAAGGGAGTGTGAGGAGGAGACACTATAGAGGAGTGAGGCAGAGACCATGGGGAAAGTTTGCTGCCGAGATTCGTGATCCAAAGAAGGCAGCCCGAGTGTGGCTTGGAACTTTTGAAACGGCGGAGGCTGCTGCACTTGCTTACGACGAAGCAGCTCTCAAATTCAAAGGAAGCAAGGCTAAGCTTAACTTCCCGGAAAGAGTGCAAGGGACGAGTGAATTAGGCGGTTACCTCACAACTAACACAGTTGCTCATCAGCCTCTCATTTCTGATTATCATCATCAGCAACTGGTATACCCGAATAATATCCCTAATACTGGTCAATATTATCCACAATTTTATGGGAACTTAAACTATGGTCAGACAGATCAAAGAATTTATTACCAGCCTACTACGTCTTCATCTCCACCTTTTATACCAATATCTCAAGCACAAGAAGAAGATCAACAACAACAGCAGGCCTTAAGCTTTCCTATGCCGGCGTTTGGTTCATCTTCTTCCAATTTGGATCCTGAATATCCACAGAACAATACTGCAAGAAATTTTGACAATAGTCATTCGAGAGGATAG

Protein Analysis

378

Amino Acids

41.76

Weight (kDa)

6.35

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 197 - 246 1.6e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011721)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43160
fragaria_vesca FvH4_5g09511 FvH4_5g09511
malus_domestica MD06G1130400.v1.1 MD14G1147200.v1.1
prunus_persica Prupe.5G141300_v2.0.a1
pyrus_communis pycom06g12220 pycom14g12180 pycom14g12190
rosa_chinensis RchiOBHm_Chr7g0188691
rosa_laevigata RLG00000004669
rosa_multiflora Rmu_sc0001576.1_g000029
rosa_roxburghii Rroxscaffold_3G00266130
rosa_rugosa Rorug06G0492300
rosa_samantha Rh7AG097200 Rh7BG099000 Rh7CG100800 Rh7DG099500
rosa_wichuraiana Rw7G008410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 273
AccB1I GGYRCC 3 cut(s) 50, 379, 521
AccI GTMKAC 1 cut(s) 861
AccII CGCG 1 cut(s) 120
AclWI GGATC 5 cut(s) 331, 363, 638, 1070, 1083
AcoI YGGCCR 2 cut(s) 31, 39
AcsI RAATTY 4 cut(s) 252, 728, 942, 1108
AcyI GRCGYC 4 cut(s) 24, 93, 380, 522
AfaI GTAC 1 cut(s) 475
AfiI CCNNNNNNNGG 5 cut(s) 320, 569, 666, 884, 1052
AflII CTTAAG 1 cut(s) 1030
AgsI TTSAA 3 cut(s) 503, 683, 733
AhlI ACTAGT 1 cut(s) 235
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 1 cut(s) 218
AluBI AGCT 7 cut(s) 199, 228, 407, 443, 722, 751, 1035
AluI AGCT 7 cut(s) 199, 228, 407, 443, 722, 751, 1035
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 3 cut(s) 442, 580, 604
AlwI GGATC 5 cut(s) 331, 363, 638, 1070, 1083
AlwNI CAGNNNCTG 1 cut(s) 856
Ama87I CYCGRG 1 cut(s) 660
AoxI GGCC 5 cut(s) 31, 39, 130, 306, 1026
ApeKI GCWGC 7 cut(s) 12, 161, 629, 656, 695, 698, 719
ApoI RAATTY 4 cut(s) 252, 728, 942, 1108
Asp700I GAANNNNTTC 1 cut(s) 227
AspLEI GCGC 3 cut(s) 382, 524, 562
AspS9I GGNCC 1 cut(s) 306
AsuC2I CCSGG 1 cut(s) 761
AsuHPI GGTGA 2 cut(s) 95, 271
AvaI CYCGRG 1 cut(s) 660
BamHI GGATCC 1 cut(s) 1075
BanI GGYRCC 3 cut(s) 50, 379, 521
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 957
Bbv12I GWGCWC 1 cut(s) 192
BbvCI CCTCAGC 1 cut(s) 403
BbvI GCAGC 6 cut(s) 148, 616, 668, 682, 685, 731
BccI CCATC 2 cut(s) 314, 558
BceAI ACGGC 8 cut(s) 26, 54, 80, 82, 145, 367, 512, 702
BciT130I CCWGG 1 cut(s) 220
BclI TGATCA 2 cut(s) 276, 541
BcnI CCSGG 1 cut(s) 761
BcoDI GTCTC 3 cut(s) 442, 580, 604
BcuI ACTAGT 1 cut(s) 235
BfaI CTAG 1 cut(s) 236
BfmI CTRYAG 1 cut(s) 592
BfoI RGCGCY 3 cut(s) 383, 525, 563
BfrI CTTAAG 1 cut(s) 1030
BfuAI ACCTGC 1 cut(s) 273
BlpI GCTNAGC 1 cut(s) 747
BmcAI AGTACT 1 cut(s) 475
Bme1390I CCNGG 2 cut(s) 220, 761
BmeT110I CYCGRG 1 cut(s) 660
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 306
BmiI GGNNCC 4 cut(s) 52, 381, 523, 1077
BmrFI CCNGG 2 cut(s) 220, 761
BoxI GACNNNNGTC 2 cut(s) 41, 1119
BpiI GAAGAC 1 cut(s) 957
Bpu10I CCTNAGC 1 cut(s) 403
Bpu1102I GCTNAGC 1 cut(s) 747
BpuEI CTTGAG 2 cut(s) 95, 978
BpuMI CCSGG 1 cut(s) 761
BsaBI GATNNNNATC 1 cut(s) 843
BsaHI GRCGYC 4 cut(s) 24, 93, 380, 522
BsaI GGTCTC 2 cut(s) 442, 604
BsaJI CCNNGG 5 cut(s) 118, 127, 156, 432, 614
BsaXI ACNNNNNCTCC 3 cut(s) 29, 176, 206
Bsc4I CCNNNNNNNGG 5 cut(s) 320, 569, 666, 884, 1052
Bse118I RCCGGY 2 cut(s) 26, 1045
Bse1I ACTGG 3 cut(s) 215, 861, 889
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 1 cut(s) 220
BseDI CCNNGG 5 cut(s) 118, 127, 156, 432, 614
BseGI GGATG 1 cut(s) 398
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 5 cut(s) 320, 569, 666, 884, 1052
BseMII CTCAG 3 cut(s) 155, 417, 453
BseNI ACTGG 3 cut(s) 215, 861, 889
BseRI GAGGAG 5 cut(s) 98, 344, 595, 598, 612
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 6 cut(s) 148, 616, 668, 682, 685, 731
BseYI CCCAGC 2 cut(s) 268, 373
BsgI GTGCAG 2 cut(s) 480, 684
Bsh1236I CGCG 1 cut(s) 120
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 5 cut(s) 33, 41, 132, 308, 1028
BshNI GGYRCC 3 cut(s) 50, 379, 521
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 660
BsiSI CCGG 4 cut(s) 27, 519, 761, 1046
BslFI GGGAC 2 cut(s) 197, 790
BslI CCNNNNNNNGG 5 cut(s) 320, 569, 666, 884, 1052
BsmAI GTCTC 3 cut(s) 442, 580, 604
BsmFI GGGAC 2 cut(s) 197, 790
BsnI GGCC 5 cut(s) 33, 41, 132, 308, 1028
Bso31I GGTCTC 2 cut(s) 442, 604
BsoBI CYCGRG 1 cut(s) 660
Bsp1286I GDGCHC 1 cut(s) 192
Bsp1720I GCTNAGC 1 cut(s) 747
Bsp19I CCATGG 2 cut(s) 432, 614
BspANI GGCC 5 cut(s) 33, 41, 132, 308, 1028
BspCNI CTCAG 3 cut(s) 154, 416, 452
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 4 cut(s) 52, 381, 523, 1077
BspMI ACCTGC 1 cut(s) 273
BspPI GGATC 5 cut(s) 331, 363, 638, 1070, 1083
BspT107I GGYRCC 3 cut(s) 50, 379, 521
BspTI CTTAAG 1 cut(s) 1030
BspTNI GGTCTC 2 cut(s) 442, 604
BsrFI RCCGGY 2 cut(s) 26, 1045
BsrI ACTGG 3 cut(s) 215, 861, 889
BssAI RCCGGY 2 cut(s) 26, 1045
BssECI CCNNGG 5 cut(s) 118, 127, 156, 432, 614
BssNAI GTATAC 1 cut(s) 862
BssNI GRCGYC 4 cut(s) 24, 93, 380, 522
BssSI CACGAG 1 cut(s) 315
BssT1I CCWWGG 3 cut(s) 156, 432, 614
Bst1107I GTATAC 1 cut(s) 862
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 220
Bst4CI ACNGT 3 cut(s) 470, 516, 814
Bst6I CTCTTC 3 cut(s) 140, 296, 336
BstACI GRCGYC 4 cut(s) 24, 93, 380, 522
BstAFI CTTAAG 1 cut(s) 1030
BstC8I GCNNGC 3 cut(s) 28, 1026, 1047
BstDEI CTNAG 4 cut(s) 141, 403, 439, 747
BstDSI CCRYGG 4 cut(s) 118, 127, 432, 614
BstEII GGTNACC 1 cut(s) 794
BstF5I GGATG 1 cut(s) 398
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 3 cut(s) 383, 525, 563
BstHHI GCGC 3 cut(s) 382, 524, 562
BstMAI GTCTC 3 cut(s) 442, 580, 604
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 4 cut(s) 129, 196, 695, 704
BstNI CCWGG 1 cut(s) 220
BstPAI GACNNNNGTC 2 cut(s) 41, 1119
BstPI GGTNACC 1 cut(s) 794
BstSCI CCNGG 2 cut(s) 218, 759
BstSFI CTRYAG 1 cut(s) 592
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 6 cut(s) 148, 616, 668, 682, 685, 731
BstV2I GAAGAC 1 cut(s) 957
BstX2I RGATCY 1 cut(s) 1075
BstYI RGATCY 1 cut(s) 1075
BstZ17I GTATAC 1 cut(s) 862
BstZI CGGCCG 1 cut(s) 39
BsuRI GGCC 5 cut(s) 33, 41, 132, 308, 1028
BtgI CCRYGG 4 cut(s) 118, 127, 432, 614
BtrI CACGTC 1 cut(s) 187
BtsCI GGATG 1 cut(s) 398
BtsIMutI CAGTG 1 cut(s) 208
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 28, 1026, 1047
CaiI CAGNNNCTG 1 cut(s) 856
CfoI GCGC 3 cut(s) 382, 524, 562
Cfr10I RCCGGY 2 cut(s) 26, 1045
Cfr13I GGNCC 1 cut(s) 306
Cfr42I CCGCGG 1 cut(s) 121
CseI GACGC 3 cut(s) 32, 89, 101
Csp6I GTAC 1 cut(s) 474
CviAII CATG 5 cut(s) 274, 296, 433, 539, 615
CviQI GTAC 1 cut(s) 474
DdeI CTNAG 4 cut(s) 141, 403, 439, 747
DinI GGCGCC 2 cut(s) 381, 523
EaeI YGGCCR 2 cut(s) 31, 39
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 3 cut(s) 140, 296, 336
EarI CTCTTC 3 cut(s) 140, 296, 336
EciI GGCGGA 1 cut(s) 704
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 3 cut(s) 156, 432, 614
Eco147I AGGCCT 1 cut(s) 1028
Eco31I GGTCTC 2 cut(s) 442, 604
Eco52I CGGCCG 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 660
Eco91I GGTNACC 1 cut(s) 794
EcoO109I RGGNCCY 1 cut(s) 306
EcoO65I GGTNACC 1 cut(s) 794
EcoRII CCWGG 1 cut(s) 218
EcoT14I CCWWGG 3 cut(s) 156, 432, 614
EgeI GGCGCC 2 cut(s) 381, 523
EheI GGCGCC 2 cut(s) 381, 523
ErhI CCWWGG 3 cut(s) 156, 432, 614
FaeI CATG 5 cut(s) 277, 299, 436, 542, 618
FalI AAGNNNNNCTT 4 cut(s) 293, 325, 740, 772
FaqI GGGAC 2 cut(s) 197, 790
FatI CATG 5 cut(s) 273, 295, 432, 538, 614
FauI CCCGC 1 cut(s) 125
FbaI TGATCA 2 cut(s) 276, 541
FblI GTMKAC 1 cut(s) 861
FokI GGATG 1 cut(s) 385
FspBI CTAG 1 cut(s) 236
GlaI GCGC 3 cut(s) 381, 523, 561
GsaI CCCAGC 2 cut(s) 272, 377
HaeII RGCGCY 3 cut(s) 383, 525, 563
HaeIII GGCC 5 cut(s) 33, 41, 132, 308, 1028
HapII CCGG 4 cut(s) 27, 519, 761, 1046
HgaI GACGC 3 cut(s) 32, 89, 101
HhaI GCGC 3 cut(s) 382, 524, 562
Hin1I GRCGYC 4 cut(s) 24, 93, 380, 522
Hin1II CATG 5 cut(s) 277, 299, 436, 542, 618
Hin6I GCGC 3 cut(s) 380, 522, 560
HinP1I GCGC 3 cut(s) 380, 522, 560
HindIII AAGCTT 3 cut(s) 226, 749, 1033
HinfI GANTC 1 cut(s) 637
HpaII CCGG 4 cut(s) 27, 519, 761, 1046
HphI GGTGA 2 cut(s) 95, 271
Hpy166II GTNNAC 2 cut(s) 241, 862
Hpy188I TCNGA 6 cut(s) 19, 144, 328, 415, 838, 930
Hpy188III TCNNGA 7 cut(s) 112, 182, 317, 353, 641, 1079, 1128
Hpy8I GTNNAC 2 cut(s) 241, 862
Hpy99I CGWCG 7 cut(s) 23, 26, 47, 83, 95, 101, 716
HpyAV CCTTC 2 cut(s) 319, 646
HpyCH4III ACNGT 3 cut(s) 470, 516, 814
HpyCH4IV ACGT 2 cut(s) 186, 962
HpyCH4V TGCA 4 cut(s) 497, 701, 772, 1103
HpyF10VI GCNNNNNNNGC 4 cut(s) 129, 196, 695, 704
HpyF3I CTNAG 4 cut(s) 141, 403, 439, 747
HpySE526I ACGT 2 cut(s) 186, 962
Hsp92I GRCGYC 4 cut(s) 24, 93, 380, 522
Hsp92II CATG 5 cut(s) 277, 299, 436, 542, 618
HspAI GCGC 3 cut(s) 380, 522, 560
KasI GGCGCC 2 cut(s) 379, 521
KroI GCCGGC 2 cut(s) 26, 1045
KroNI GCCGGC 2 cut(s) 28, 1047
Ksp22I TGATCA 2 cut(s) 276, 541
KspI CCGCGG 1 cut(s) 121
LmnI GCTCC 2 cut(s) 20, 375
Lsp1109I GCAGC 6 cut(s) 148, 616, 668, 682, 685, 731
MaeI CTAG 1 cut(s) 236
MaeII ACGT 2 cut(s) 186, 962
MaeIII GTNAC 1 cut(s) 794
MboII GAAGA 8 cut(s) 127, 313, 353, 957, 1016, 1019, 1053, 1056
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 7 cut(s) 252, 728, 785, 902, 942, 1069, 1108
Mly113I GGCGCC 2 cut(s) 380, 522
MmeI TCCRAC 1 cut(s) 42
MreI CGCCGGCG 1 cut(s) 26
MroNI GCCGGC 2 cut(s) 26, 1045
MroXI GAANNNNTTC 1 cut(s) 227
MseI TTAA 3 cut(s) 753, 917, 1031
MslI CAYNNNNRTG 1 cut(s) 236
MspA1I CMGCKG 2 cut(s) 120, 377
MspCI CTTAAG 1 cut(s) 1030
MspI CCGG 4 cut(s) 27, 519, 761, 1046
MspR9I CCNGG 2 cut(s) 220, 761
MvaI CCWGG 1 cut(s) 220
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 4 cut(s) 129, 196, 695, 704
NaeI GCCGGC 2 cut(s) 28, 1047
NarI GGCGCC 2 cut(s) 380, 522
NciI CCSGG 1 cut(s) 761
NcoI CCATGG 2 cut(s) 432, 614
NgoMIV GCCGGC 2 cut(s) 26, 1045
NlaIII CATG 5 cut(s) 277, 299, 436, 542, 618
NlaIV GGNNCC 4 cut(s) 52, 381, 523, 1077
NmeAIII GCCGAG 1 cut(s) 658
PceI AGGCCT 1 cut(s) 1028
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 96
PdiI GCCGGC 2 cut(s) 28, 1047
PdmI GAANNNNTTC 1 cut(s) 227
PfeI GAWTC 1 cut(s) 637
PflFI GACNNNGTC 1 cut(s) 95
PfoI TCCNGGA 1 cut(s) 759
PluTI GGCGCC 2 cut(s) 383, 525
PshAI GACNNNNGTC 2 cut(s) 41, 1119
Psp6I CCWGG 1 cut(s) 218
PspEI GGTNACC 1 cut(s) 794
PspFI CCCAGC 2 cut(s) 268, 373
PspGI CCWGG 1 cut(s) 218
PspN4I GGNNCC 4 cut(s) 52, 381, 523, 1077
PspPI GGNCC 1 cut(s) 306
PstNI CAGNNNCTG 1 cut(s) 856
PsuI RGATCY 1 cut(s) 1075
PsyI GACNNNGTC 1 cut(s) 95
RsaI GTAC 1 cut(s) 475
RsaNI GTAC 1 cut(s) 474
RseI CAYNNNNRTG 1 cut(s) 236
SacII CCGCGG 1 cut(s) 121
SaqAI TTAA 3 cut(s) 753, 917, 1031
Sau96I GGNCC 1 cut(s) 306
ScaI AGTACT 1 cut(s) 475
ScrFI CCNGG 2 cut(s) 220, 761
SduI GDGCHC 1 cut(s) 192
SfcI CTRYAG 1 cut(s) 592
SfoI GGCGCC 2 cut(s) 381, 523
Sfr303I CCGCGG 1 cut(s) 121
SgrAI CRCCGGYG 1 cut(s) 26
SgrBI CCGCGG 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 3 cut(s) 110, 993, 1030
SmoI CTYRAG 3 cut(s) 110, 993, 1030
SpeI ACTAGT 1 cut(s) 235
Sse9I AATT 7 cut(s) 252, 728, 785, 902, 942, 1069, 1108
SseBI AGGCCT 1 cut(s) 1028
SspDI GGCGCC 2 cut(s) 379, 521
SspI AATATT 1 cut(s) 893
SspMI CTAG 1 cut(s) 236
StuI AGGCCT 1 cut(s) 1028
StyD4I CCNGG 2 cut(s) 218, 759
StyI CCWWGG 3 cut(s) 156, 432, 614
TaaI ACNGT 3 cut(s) 470, 516, 814
TaiI ACGT 2 cut(s) 189, 965
TaqI TCGA 4 cut(s) 99, 147, 409, 1127
TasI AATT 7 cut(s) 252, 728, 785, 902, 942, 1069, 1108
TatI WGTACW 1 cut(s) 473
TauI GCSGC 6 cut(s) 33, 51, 59, 123, 380, 527
TfiI GAWTC 1 cut(s) 637
Tru1I TTAA 3 cut(s) 753, 917, 1031
Tru9I TTAA 3 cut(s) 753, 917, 1031
TscAI CASTG 1 cut(s) 215
TseI GCWGC 7 cut(s) 12, 161, 629, 656, 695, 698, 719
TspDTI ATGAA 3 cut(s) 220, 957, 1047
TspRI CASTG 1 cut(s) 215
Tth111I GACNNNGTC 1 cut(s) 95
Vha464I CTTAAG 1 cut(s) 1030
XapI RAATTY 4 cut(s) 252, 728, 942, 1108
XmiI GTMKAC 1 cut(s) 861
XmnI GAANNNNTTC 1 cut(s) 227
XspI CTAG 1 cut(s) 236
ZrmI AGTACT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.