RLG00000009439

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0013764
Physical Location & Seq
Forward (+)
52870754 .. 52872699
1946 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009439

Sequence Viewer

Length: 657 bp
ATGGGGGGACCTCCCTCTGAAAACCTATGCTCTCCCTCTCTCTGGGTGTCTCCTCGTCGTTGTCATCTCTCTCTCTCTCTCCTCGACGGCACCGGCACTCTCCCTCTCCCCTCGACCGTCGACGGCGCTCGCTCTTCTCAACAATGTTACCAGCCTTCAGATCCTGAATGGGAAGCTTTTCAAAACATAAGCAACCAAGCTCACACAAACCAATTTCTCGATTCCTTCGCCACCCCGAAGCTCTCTCTCTCTCTCATCGTTGGTGTTGTTGTTTCAAAAGCAGTTTGGAAGAGACGGAGAAGGGCCACAAGTAGTGTGAGTGTCTTCTTAGATGTAGTGTATCTGAAGAAAAGGATATTTTCAATTGTGGCATGGTACTTTTTGAGGAAGTTGAAGAAAGTGAAGAAGAGCAATGGACAAATCTTGGCCATCAATGAGGGGGTCAAAGCTGCAGAAAGATGGAAATGGGGGGTCAGAATCACAGATGGAAATGGGAGATGCAAAATCTCAAATGGGACTTCTAGTAAAGATGCTAAACTAGTGGAAAGCTCCGGCAGCACAATTGACAGTAATACTCTTTCCCTCTCTTTGGAGTATGTGCTTGCTACTGGTTCCATTTTGAGATCCTGCTTCTCCATGATTTACCTTTGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18A

Protein Analysis

219

Amino Acids

24.15

Weight (kDa)

9.87

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 120
AclWI GGATC 2 cut(s) 155, 618
AcoI YGGCCR 1 cut(s) 426
AcuI CTGAAG 2 cut(s) 141, 365
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 2 cut(s) 42, 589
AgsI TTSAA 4 cut(s) 182, 276, 363, 394
AhlI ACTAGT 1 cut(s) 538
AluBI AGCT 5 cut(s) 176, 200, 241, 449, 549
AluI AGCT 5 cut(s) 176, 200, 241, 449, 549
Alw26I GTCTC 2 cut(s) 54, 286
AlwI GGATC 2 cut(s) 155, 618
AlwNI CAGNNNCTG 1 cut(s) 164
AoxI GGCC 2 cut(s) 303, 426
ApeKI GCWGC 2 cut(s) 449, 555
Asp700I GAANNNNTTC 1 cut(s) 177
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 2 cut(s) 8, 303
AvaII GGWCC 1 cut(s) 8
BalI TGGCCA 1 cut(s) 428
BanI GGYRCC 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 316
BbvI GCAGC 2 cut(s) 436, 567
BccI CCATC 3 cut(s) 437, 453, 479
BceAI ACGGC 2 cut(s) 103, 139
BcoDI GTCTC 2 cut(s) 54, 286
BcuI ACTAGT 1 cut(s) 538
BfaI CTAG 2 cut(s) 522, 539
BfmI CTRYAG 1 cut(s) 450
BfoI RGCGCY 1 cut(s) 129
BisI GCNGC 2 cut(s) 450, 556
BlsI GCNGC 2 cut(s) 451, 557
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 8, 303
BmiI GGNNCC 3 cut(s) 9, 91, 613
BmsI GCATC 2 cut(s) 488, 520
BpiI GAAGAC 1 cut(s) 316
Bsc4I CCNNNNNNNGG 2 cut(s) 42, 589
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 613
Bse3DI GCAATG 1 cut(s) 418
BseLI CCNNNNNNNGG 2 cut(s) 42, 589
BseMI GCAATG 1 cut(s) 418
BseNI ACTGG 1 cut(s) 613
BseRI GAGGAG 2 cut(s) 42, 71
BseXI GCAGC 2 cut(s) 436, 567
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 2 cut(s) 305, 428
BshNI GGYRCC 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 117
BsiSI CCGG 2 cut(s) 93, 552
BslFI GGGAC 2 cut(s) 21, 529
BslI CCNNNNNNNGG 2 cut(s) 42, 589
BsmAI GTCTC 2 cut(s) 54, 286
BsmBI CGTCTC 1 cut(s) 286
BsmFI GGGAC 2 cut(s) 21, 529
BsnI GGCC 2 cut(s) 305, 428
Bsp143I GATC 2 cut(s) 160, 623
BspANI GGCC 2 cut(s) 305, 428
BspLI GGNNCC 3 cut(s) 9, 91, 613
BspMAI CTGCAG 1 cut(s) 454
BspPI GGATC 2 cut(s) 155, 618
BspQI GCTCTTC 2 cut(s) 139, 401
BspT107I GGYRCC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 1 cut(s) 613
BssAI RCCGGY 1 cut(s) 92
BssMI GATC 2 cut(s) 160, 623
Bst4CI ACNGT 2 cut(s) 118, 569
Bst6I CTCTTC 3 cut(s) 139, 284, 401
BstC8I GCNNGC 2 cut(s) 130, 603
BstDEI CTNAG 1 cut(s) 328
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 2 cut(s) 163, 626
BstMAI GTCTC 2 cut(s) 54, 286
BstMBI GATC 2 cut(s) 160, 623
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 1 cut(s) 555
BstSFI CTRYAG 1 cut(s) 450
BstV1I GCAGC 2 cut(s) 436, 567
BstV2I GAAGAC 1 cut(s) 316
BstX2I RGATCY 2 cut(s) 160, 623
BstYI RGATCY 2 cut(s) 160, 623
BsuRI GGCC 2 cut(s) 305, 428
Cac8I GCNNGC 2 cut(s) 130, 603
CaiI CAGNNNCTG 1 cut(s) 164
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 8, 303
Csp6I GTAC 1 cut(s) 376
CviAII CATG 2 cut(s) 372, 637
CviJI RGCY 8 cut(s) 154, 176, 200, 241, 305, 428, 449, 549
CviKI_1 RGCY 8 cut(s) 154, 176, 200, 241, 305, 428, 449, 549
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 1 cut(s) 328
DpnI GATC 2 cut(s) 162, 625
DpnII GATC 2 cut(s) 160, 623
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 3 cut(s) 139, 284, 401
EarI CTCTTC 3 cut(s) 139, 284, 401
Eco47I GGWCC 1 cut(s) 8
Eco57I CTGAAG 2 cut(s) 141, 365
EcoO109I RGGNCCY 1 cut(s) 8
Esp3I CGTCTC 1 cut(s) 286
FaeI CATG 2 cut(s) 375, 640
FaiI YATR 5 cut(s) 28, 188, 373, 597, 638
FaqI GGGAC 2 cut(s) 21, 529
FatI CATG 2 cut(s) 371, 636
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 2 cut(s) 450, 556
Fsp4HI GCNGC 2 cut(s) 450, 556
FspBI CTAG 2 cut(s) 522, 539
GlaI GCGC 1 cut(s) 127
GluI GCNGC 2 cut(s) 450, 556
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 2 cut(s) 305, 428
HapII CCGG 2 cut(s) 93, 552
HhaI GCGC 1 cut(s) 128
Hin1II CATG 2 cut(s) 375, 640
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 174
HinfI GANTC 2 cut(s) 221, 477
HpaII CCGG 2 cut(s) 93, 552
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 4 cut(s) 19, 160, 345, 476
Hpy188III TCNNGA 2 cut(s) 164, 218
Hpy8I GTNNAC 1 cut(s) 121
Hpy99I CGWCG 4 cut(s) 60, 89, 122, 125
HpyAV CCTTC 3 cut(s) 165, 235, 294
HpyCH4III ACNGT 2 cut(s) 118, 569
HpyCH4V TGCA 2 cut(s) 452, 501
HpyF10VI GCNNNNNNNGC 1 cut(s) 555
HpyF3I CTNAG 1 cut(s) 328
Hsp92II CATG 2 cut(s) 375, 640
HspAI GCGC 1 cut(s) 126
Kzo9I GATC 2 cut(s) 160, 623
LguI GCTCTTC 2 cut(s) 139, 401
LmnI GCTCC 1 cut(s) 554
LpnPI CCDG 7 cut(s) 28, 106, 164, 177, 565, 594, 640
Lsp1109I GCAGC 2 cut(s) 436, 567
LweI GCATC 2 cut(s) 488, 520
MaeI CTAG 2 cut(s) 522, 539
MaeIII GTNAC 1 cut(s) 146
MalI GATC 2 cut(s) 162, 625
MboI GATC 2 cut(s) 160, 623
MboII GAAGA 7 cut(s) 126, 301, 316, 358, 406, 415, 418
MfeI CAATTG 2 cut(s) 363, 561
MflI RGATCY 2 cut(s) 160, 623
MlsI TGGCCA 1 cut(s) 428
MluCI AATT 3 cut(s) 212, 363, 561
MluNI TGGCCA 1 cut(s) 428
Mox20I TGGCCA 1 cut(s) 428
MroXI GAANNNNTTC 1 cut(s) 177
MscI TGGCCA 1 cut(s) 428
Msp20I TGGCCA 1 cut(s) 428
MspI CCGG 2 cut(s) 93, 552
MunI CAATTG 2 cut(s) 363, 561
MwoI GCNNNNNNNGC 1 cut(s) 555
NdeII GATC 2 cut(s) 160, 623
NlaIII CATG 2 cut(s) 375, 640
NlaIV GGNNCC 3 cut(s) 9, 91, 613
PciSI GCTCTTC 2 cut(s) 139, 401
PdmI GAANNNNTTC 1 cut(s) 177
PfeI GAWTC 2 cut(s) 221, 477
PkrI GCNGC 2 cut(s) 451, 557
PpuMI RGGWCCY 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 8
PspN4I GGNNCC 3 cut(s) 9, 91, 613
PspPI GGNCC 2 cut(s) 8, 303
PspPPI RGGWCCY 1 cut(s) 8
PstI CTGCAG 1 cut(s) 454
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 2 cut(s) 160, 623
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
SalI GTCGAC 1 cut(s) 119
SapI GCTCTTC 2 cut(s) 139, 401
SatI GCNGC 2 cut(s) 450, 556
Sau3AI GATC 2 cut(s) 160, 623
Sau96I GGNCC 2 cut(s) 8, 303
SetI ASST 8 cut(s) 13, 27, 178, 202, 243, 451, 551, 648
SfaNI GCATC 2 cut(s) 488, 520
SfcI CTRYAG 1 cut(s) 450
SgrDI CGTCGACG 1 cut(s) 119
SinI GGWCC 1 cut(s) 8
SpeI ACTAGT 1 cut(s) 538
Sse9I AATT 3 cut(s) 212, 363, 561
SspMI CTAG 2 cut(s) 522, 539
TaaI ACNGT 2 cut(s) 118, 569
TaqI TCGA 4 cut(s) 84, 113, 120, 219
TasI AATT 3 cut(s) 212, 363, 561
TfiI GAWTC 2 cut(s) 221, 477
TseI GCWGC 2 cut(s) 449, 555
TspGWI ACGGA 1 cut(s) 310
VpaK11BI GGWCC 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 2 cut(s) 522, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.