Rh5BG474500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0013764
Physical Location & Seq
Reverse (-)
75403334 .. 75404246
913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG474500.1

Sequence Viewer

Length: 354 bp
ATGGACAAATCTTGGCCATCAATGAGCTCTGCGTCCGCAATTGATAACAAGGAACAAGGCATTCCTACAGATTTTATGATGAGCATCCAGTTAGAGTCACACAAAATGGAAATGGGGGGGTCAGAGCTGCAAAAAGATGGAAATGGGGGGTCAGAATCACAGAAAGATGGAAATGGGAGATGCAAAATCTCAAATGGGACTTCTAGGAAAGATCCTAAACTAGTGGAAAGATCCGGCAGCACAATTGACGGTAATACTCTTTCCCCTCTCTCTGGAGTATGTGCTTGCCACTGGTTCCATTTTGAGATCCTGCTTCTCCATGATTTACCTTTGAACTTTATTTTGCTTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

117

Amino Acids

12.83

Weight (kDa)

5.74

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 36
AclWI GGATC 3 cut(s) 206, 225, 301
AcoI YGGCCR 1 cut(s) 14
AfiI CCNNNNNNNGG 1 cut(s) 272
AgsI TTSAA 1 cut(s) 334
AhlI ACTAGT 1 cut(s) 220
AluBI AGCT 2 cut(s) 27, 127
AluI AGCT 2 cut(s) 27, 127
Alw21I GWGCWC 1 cut(s) 29
AlwI GGATC 3 cut(s) 206, 225, 301
AoxI GGCC 1 cut(s) 14
ApeKI GCWGC 2 cut(s) 127, 237
BalI TGGCCA 1 cut(s) 16
BanII GRGCYC 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 2 cut(s) 114, 249
BccI CCATC 3 cut(s) 25, 131, 161
BcuI ACTAGT 1 cut(s) 220
BfaI CTAG 2 cut(s) 204, 221
BfmI CTRYAG 1 cut(s) 66
BisI GCNGC 2 cut(s) 128, 238
BlsI GCNGC 2 cut(s) 129, 239
BmiI GGNNCC 1 cut(s) 296
BmsI GCATC 2 cut(s) 93, 170
BpmI CTGGAG 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 347
BsaBI GATNNNNATC 1 cut(s) 83
Bsc4I CCNNNNNNNGG 1 cut(s) 272
Bse1I ACTGG 2 cut(s) 88, 296
Bse8I GATNNNNATC 1 cut(s) 83
BseGI GGATG 1 cut(s) 84
BseJI GATNNNNATC 1 cut(s) 83
BseLI CCNNNNNNNGG 1 cut(s) 272
BseNI ACTGG 2 cut(s) 88, 296
BseXI GCAGC 2 cut(s) 114, 249
BshFI GGCC 1 cut(s) 16
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 211
BsmI GAATGC 1 cut(s) 60
BsnI GGCC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 29
Bsp143I GATC 3 cut(s) 211, 230, 306
BspACI CCGC 1 cut(s) 36
BspANI GGCC 1 cut(s) 16
BspLI GGNNCC 1 cut(s) 296
BspPI GGATC 3 cut(s) 206, 225, 301
BsrI ACTGG 2 cut(s) 88, 296
BssMI GATC 3 cut(s) 211, 230, 306
Bst4CI ACNGT 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 286
BstDEI CTNAG 1 cut(s) 347
BstF5I GGATG 1 cut(s) 84
BstKTI GATC 3 cut(s) 214, 233, 309
BstMBI GATC 3 cut(s) 211, 230, 306
BstSFI CTRYAG 1 cut(s) 66
BstV1I GCAGC 2 cut(s) 114, 249
BstX2I RGATCY 3 cut(s) 211, 230, 306
BstYI RGATCY 3 cut(s) 211, 230, 306
BsuRI GGCC 1 cut(s) 16
BtsCI GGATG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 289
Cac8I GCNNGC 1 cut(s) 286
CseI GACGC 1 cut(s) 21
CviAII CATG 1 cut(s) 320
CviJI RGCY 3 cut(s) 16, 27, 127
CviKI_1 RGCY 3 cut(s) 16, 27, 127
DdeI CTNAG 1 cut(s) 347
DpnI GATC 3 cut(s) 213, 232, 308
DpnII GATC 3 cut(s) 211, 230, 306
EaeI YGGCCR 1 cut(s) 14
Ecl136II GAGCTC 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 29
Eco53kI GAGCTC 1 cut(s) 27
EcoICRI GAGCTC 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 29
FaeI CATG 1 cut(s) 323
FaiI YATR 3 cut(s) 77, 280, 321
FaqI GGGAC 1 cut(s) 211
FatI CATG 1 cut(s) 319
Fnu4HI GCNGC 2 cut(s) 128, 238
FokI GGATG 1 cut(s) 71
FriOI GRGCYC 1 cut(s) 29
Fsp4HI GCNGC 2 cut(s) 128, 238
FspBI CTAG 2 cut(s) 204, 221
GluI GCNGC 2 cut(s) 128, 238
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 1 cut(s) 16
HapII CCGG 1 cut(s) 234
HgaI GACGC 1 cut(s) 21
Hin1II CATG 1 cut(s) 323
HinfI GANTC 2 cut(s) 95, 155
HpaII CCGG 1 cut(s) 234
Hpy188I TCNGA 2 cut(s) 124, 154
Hpy188III TCNNGA 1 cut(s) 273
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4V TGCA 2 cut(s) 130, 183
HpyF3I CTNAG 1 cut(s) 347
Hsp92II CATG 1 cut(s) 323
Kzo9I GATC 3 cut(s) 211, 230, 306
LpnPI CCDG 5 cut(s) 101, 247, 258, 277, 323
Lsp1109I GCAGC 2 cut(s) 114, 249
LweI GCATC 2 cut(s) 93, 170
MaeI CTAG 2 cut(s) 204, 221
MaeIII GTNAC 1 cut(s) 96
MalI GATC 3 cut(s) 213, 232, 308
MboI GATC 3 cut(s) 211, 230, 306
MfeI CAATTG 2 cut(s) 39, 243
MflI RGATCY 3 cut(s) 211, 230, 306
MhlI GDGCHC 1 cut(s) 29
MlsI TGGCCA 1 cut(s) 16
MluCI AATT 2 cut(s) 39, 243
MluNI TGGCCA 1 cut(s) 16
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 1 cut(s) 276
Mox20I TGGCCA 1 cut(s) 16
MscI TGGCCA 1 cut(s) 16
Msp20I TGGCCA 1 cut(s) 16
MspI CCGG 1 cut(s) 234
MunI CAATTG 2 cut(s) 39, 243
Mva1269I GAATGC 1 cut(s) 60
NdeII GATC 3 cut(s) 211, 230, 306
NlaIII CATG 1 cut(s) 323
NlaIV GGNNCC 1 cut(s) 296
NmuCI GTSAC 1 cut(s) 96
PctI GAATGC 1 cut(s) 60
PfeI GAWTC 1 cut(s) 155
PkrI GCNGC 2 cut(s) 129, 239
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 29
PspN4I GGNNCC 1 cut(s) 296
PsuI RGATCY 3 cut(s) 211, 230, 306
SacI GAGCTC 1 cut(s) 29
SatI GCNGC 2 cut(s) 128, 238
Sau3AI GATC 3 cut(s) 211, 230, 306
SchI GAGTC 1 cut(s) 104
SduI GDGCHC 1 cut(s) 29
SetI ASST 4 cut(s) 29, 129, 331, 353
SfaNI GCATC 2 cut(s) 93, 170
SfcI CTRYAG 1 cut(s) 66
SpeI ACTAGT 1 cut(s) 220
Sse9I AATT 2 cut(s) 39, 243
SsiI CCGC 1 cut(s) 36
SspMI CTAG 2 cut(s) 204, 221
SstI GAGCTC 1 cut(s) 29
TaaI ACNGT 1 cut(s) 251
TasI AATT 2 cut(s) 39, 243
TfiI GAWTC 1 cut(s) 155
TscAI CASTG 1 cut(s) 296
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 2 cut(s) 127, 237
Tsp45I GTSAC 1 cut(s) 96
TspRI CASTG 1 cut(s) 296
XspI CTAG 2 cut(s) 204, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.