RLG00000013233

DNA topoisomerase type II (ATP-hydrolyzing) activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009658
Physical Location & Seq
Forward (+)
28104770 .. 28105213
444 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013233

Sequence Viewer

Length: 354 bp
ATGGCAGAGAGAATGTTCAAGAAACCCGTGTATGGGCTTTTTGATTTAAATCCATATGGGGCTAGAATTCTATGTATTTATCATTACGGCTCAGAAAACATGTCATATGATCGCAATAGTTTGGCCACCCCGGGTGTAAGGACAGAAGACTTAGAAACTTACGAAATACCTCAAGATGTCAAGTATAGGGTAAAGATGACTCAAACAGATAACAACGAGTGCCAGTGTTTGTTAAAAGCGAAATATTTTGTGAACAATGTAGAATGGCAGAATGAGCTGGAAGCTGGCAGAAAAACTGAAATCGAGGCATCGTATGTCCATGGTTACAAATTTTTTGCTGAGAAGAGACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000271 GO:0000280 GO:0000706 GO:0000729 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003844 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005977 GO:0005978 GO:0006073 GO:0006091 GO:0006112 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0007275 GO:0007568 GO:0008094 GO:0008150 GO:0008152 GO:0008340 GO:0008544 GO:0009058 GO:0009059 GO:0009250 GO:0009888 GO:0009913 GO:0009957 GO:0009987 GO:0010259 GO:0015980 GO:0016043 GO:0016051 GO:0016462 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0030154 GO:0030246 GO:0030855 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0033692 GO:0034637 GO:0034641 GO:0034645 GO:0035825 GO:0042138 GO:0042623 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045165 GO:0046483 GO:0048285 GO:0048856 GO:0048869 GO:0050896 GO:0051321 GO:0051716 GO:0055114 GO:0060429 GO:0061505 GO:0061982 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:1903046
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko04113 map00500 map01100 map01110 map04113
Pfam Domains
Protein Families
Alpha-amylase Alpha-amylase_C CBM_48 TP6A_N

Protein Analysis

118

Amino Acids

13.81

Weight (kDa)

6.13

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOP6A-Spo11_Toprim PF21180 5 - 114 3.9e-21 Topoisomerase 6 subunit A/Spo11, Toprim domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 2 cut(s) 66, 329
AfiI CCNNNNNNNGG 2 cut(s) 32, 33
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 1 cut(s) 19
AluBI AGCT 2 cut(s) 277, 284
AluI AGCT 2 cut(s) 277, 284
Alw26I GTCTC 1 cut(s) 340
Ama87I CYCGRG 1 cut(s) 130
AoxI GGCC 1 cut(s) 123
ApoI RAATTY 2 cut(s) 66, 329
AsuC2I CCSGG 2 cut(s) 131, 132
AvaI CYCGRG 1 cut(s) 130
BalI TGGCCA 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 153
BceAI ACGGC 1 cut(s) 103
BcnI CCSGG 2 cut(s) 131, 132
BcoDI GTCTC 1 cut(s) 340
BfaI CTAG 2 cut(s) 63, 352
Bme1390I CCNGG 2 cut(s) 131, 132
BmeT110I CYCGRG 1 cut(s) 130
BmrFI CCNGG 2 cut(s) 131, 132
BmsI GCATC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 156
BpuMI CCSGG 2 cut(s) 131, 132
BsaBI GATNNNNATC 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 340
BsaJI CCNNGG 3 cut(s) 129, 130, 319
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 33
Bse1I ACTGG 1 cut(s) 223
Bse8I GATNNNNATC 1 cut(s) 48
BseDI CCNNGG 3 cut(s) 129, 130, 319
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 2 cut(s) 32, 33
BseMII CTCAG 2 cut(s) 105, 330
BseNI ACTGG 1 cut(s) 223
BshFI GGCC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 130
BsiSI CCGG 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 32, 33
BsmAI GTCTC 1 cut(s) 340
BsnI GGCC 1 cut(s) 125
Bso31I GGTCTC 1 cut(s) 340
BsoBI CYCGRG 1 cut(s) 130
Bsp143I GATC 1 cut(s) 109
Bsp19I CCATGG 1 cut(s) 319
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 2 cut(s) 104, 331
BspTNI GGTCTC 1 cut(s) 340
BsrI ACTGG 1 cut(s) 223
BssECI CCNNGG 3 cut(s) 129, 130, 319
BssMI GATC 1 cut(s) 109
BssT1I CCWWGG 1 cut(s) 319
Bst6I CTCTTC 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 286
BstDEI CTNAG 3 cut(s) 91, 151, 339
BstDSI CCRYGG 1 cut(s) 319
BstKTI GATC 1 cut(s) 112
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 1 cut(s) 109
BstMWI GCNNNNNNNGC 1 cut(s) 274
BstNSI RCATGY 1 cut(s) 103
BstSCI CCNGG 2 cut(s) 129, 130
BstV2I GAAGAC 1 cut(s) 153
BsuRI GGCC 1 cut(s) 125
BtgI CCRYGG 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 230
Cac8I GCNNGC 1 cut(s) 286
Cfr9I CCCGGG 1 cut(s) 130
CviAII CATG 2 cut(s) 100, 320
CviJI RGCY 6 cut(s) 37, 62, 90, 125, 277, 284
CviKI_1 RGCY 6 cut(s) 37, 62, 90, 125, 277, 284
DdeI CTNAG 3 cut(s) 91, 151, 339
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
DraI TTTAAA 1 cut(s) 48
EaeI YGGCCR 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco130I CCWWGG 1 cut(s) 319
Eco31I GGTCTC 1 cut(s) 340
Eco88I CYCGRG 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 2 cut(s) 103, 323
FatI CATG 2 cut(s) 99, 319
FauNDI CATATG 2 cut(s) 55, 106
FspBI CTAG 2 cut(s) 63, 352
HaeIII GGCC 1 cut(s) 125
HapII CCGG 1 cut(s) 131
Hin1II CATG 2 cut(s) 103, 323
HinfI GANTC 1 cut(s) 199
HpaII CCGG 1 cut(s) 131
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 2 cut(s) 19, 173
Hpy8I GTNNAC 1 cut(s) 253
HpyF10VI GCNNNNNNNGC 1 cut(s) 274
HpyF3I CTNAG 3 cut(s) 91, 151, 339
Hsp92II CATG 2 cut(s) 103, 323
Kzo9I GATC 1 cut(s) 109
LpnPI CCDG 4 cut(s) 144, 236, 263, 270
LweI GCATC 1 cut(s) 317
MaeI CTAG 2 cut(s) 63, 352
MaeIII GTNAC 1 cut(s) 323
MalI GATC 1 cut(s) 111
MboI GATC 1 cut(s) 109
MboII GAAGA 1 cut(s) 158
MlsI TGGCCA 1 cut(s) 125
MluCI AATT 2 cut(s) 66, 329
MluNI TGGCCA 1 cut(s) 125
MlyI GAGTC 1 cut(s) 193
MnlI CCTC 2 cut(s) 180, 298
Mox20I TGGCCA 1 cut(s) 125
MscI TGGCCA 1 cut(s) 125
MseI TTAA 2 cut(s) 47, 233
Msp20I TGGCCA 1 cut(s) 125
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 2 cut(s) 131, 132
MwoI GCNNNNNNNGC 1 cut(s) 274
NciI CCSGG 2 cut(s) 131, 132
NcoI CCATGG 1 cut(s) 319
NdeI CATATG 2 cut(s) 55, 106
NdeII GATC 1 cut(s) 109
NlaIII CATG 2 cut(s) 103, 323
NspI RCATGY 1 cut(s) 103
PciI ACATGT 1 cut(s) 99
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
PscI ACATGT 1 cut(s) 99
SaqAI TTAA 2 cut(s) 47, 233
Sau3AI GATC 1 cut(s) 109
SchI GAGTC 1 cut(s) 193
ScrFI CCNGG 2 cut(s) 131, 132
SetI ASST 3 cut(s) 172, 279, 286
SfaNI GCATC 1 cut(s) 317
SmaI CCCGGG 1 cut(s) 132
SmiI ATTTAAAT 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 171
SmoI CTYRAG 1 cut(s) 171
Sse9I AATT 2 cut(s) 66, 329
SspI AATATT 1 cut(s) 245
SspMI CTAG 2 cut(s) 63, 352
StyD4I CCNGG 2 cut(s) 129, 130
StyI CCWWGG 1 cut(s) 319
SwaI ATTTAAAT 1 cut(s) 48
TaqI TCGA 1 cut(s) 303
TasI AATT 2 cut(s) 66, 329
Tru1I TTAA 2 cut(s) 47, 233
Tru9I TTAA 2 cut(s) 47, 233
TscAI CASTG 1 cut(s) 230
TspMI CCCGGG 1 cut(s) 130
TspRI CASTG 1 cut(s) 230
XapI RAATTY 2 cut(s) 66, 329
XceI RCATGY 1 cut(s) 103
XmaI CCCGGG 1 cut(s) 130
XspI CTAG 2 cut(s) 63, 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.