RLG00000014143

Belongs to the plant dehydrin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014045
Physical Location & Seq
Reverse (-)
48013901 .. 48014747
847 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014143

Sequence Viewer

Length: 750 bp
ATGGCTGAAATTCGCGACGAGTTCGGCAACCCCATCCCGCTCACCGACCAGTATGGCAACCCAGTTCAGCTGACAGACGAGCAAGGCCGCCCCATGCACCTCACTGGTATCGCCACCACCGAGGGCGGCCAAAGCCAAGCCATCCGTACTGGAGAGCAGCAGACCGGTATCGGAACCAGAGGCACCGGTACACACGTCCCTGGCACCGGTCTCCACGTTCCGGGCACGGGCACACACGTTCCGGGCACCGGTGCCCACATCCCCGGGACTGGCATACACATCCCCGGCACAGGTTCTCATGTTCCGGGGACAGGTGTACATGTTCCGGGCACCGGTGAGCACATGCCAGGAACCGGAGCCGGTAGCCACCCGTCAGGTACGGAGACGACCGGTGGTGTTGCTGGAGTGAAGGCTGACGAAGTTATCACCGAGCATGCCGTGCATGATACTGGAAAACCCAAAACTGTAGGTGAACATCTACAACAGGAGCGCCACACCGGTGAGGCCGGTGAACACCGTCGCAGCTCGAGCTCTAGTTCTTCGGAGGAGGATGATGGGCAAGGAGGGAGGATCAGGAAGAAGAAGGGACTGAAGGAGAAGATAAAGGAAACCTTTAGCGGTAAACATAAGGATGAGCATCCGGAGGCGGGGCCGGTCTTCAAGACCCCCACGACGACTCCTCAGGAGCATGAGAAGAAAGGCATGATGGAGAAGATCAAGGAGAAGTTACCTGGTCACCATCCCCACTGA

Protein Analysis

250

Amino Acids

26.05

Weight (kDa)

6.49

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dehydrin PF00257 22 - 247 2.4e-30 Dehydrin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21490 AT4G39130
fragaria_vesca FvH4_2g09610
malus_domestica MD10G1069000.v1.1
prunus_persica Prupe.8G091000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0265971
rosa_laevigata RLG00000014143
rosa_multiflora Rmu_co8443205.1_g000001 Rmu_sc0013338.1_g000002
rosa_roxburghii Rroxscaffold_7G00202180
rosa_rugosa Rorug06G0021900
rosa_samantha Rh6AG143200 Rh6BG143500 Rh6CG140100 Rh6DG127800
rosa_wichuraiana Rw0G015570 Rw6G012380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 182, 203, 245, 251, 329
AccBSI CCGCTC 1 cut(s) 40
AccII CGCG 1 cut(s) 15
AccIII TCCGGA 1 cut(s) 640
AciI CCGC 5 cut(s) 38, 88, 126, 618, 647
AclWI GGATC 1 cut(s) 578
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 9
AcuI CTGAAG 1 cut(s) 611
AfaI GTAC 4 cut(s) 148, 190, 318, 379
AflIII ACRYGT 3 cut(s) 193, 235, 319
AgeI ACCGGT 7 cut(s) 164, 185, 206, 248, 332, 389, 497
AgsI TTSAA 1 cut(s) 661
AjiI CACGTC 1 cut(s) 196
AjnI CCWGG 3 cut(s) 199, 346, 730
AleI CACNNNNGTG 1 cut(s) 498
AluBI AGCT 3 cut(s) 70, 525, 531
AluI AGCT 3 cut(s) 70, 525, 531
Alw21I GWGCWC 2 cut(s) 342, 533
Alw26I GTCTC 2 cut(s) 215, 377
AlwI GGATC 1 cut(s) 578
Ama87I CYCGRG 2 cut(s) 263, 526
Aor13HI TCCGGA 1 cut(s) 640
AoxI GGCC 4 cut(s) 85, 127, 504, 650
ApeKI GCWGC 2 cut(s) 157, 522
ApoI RAATTY 1 cut(s) 9
AsiGI ACCGGT 7 cut(s) 164, 185, 206, 248, 332, 389, 497
AspLEI GCGC 1 cut(s) 492
AspS9I GGNCC 1 cut(s) 650
AsuC2I CCSGG 7 cut(s) 222, 243, 264, 265, 285, 306, 327
AsuHPI GGTGA 7 cut(s) 34, 347, 418, 482, 512, 521, 728
AvaI CYCGRG 2 cut(s) 263, 526
AxyI CCTNAGG 1 cut(s) 681
BaeGI GKGCMC 5 cut(s) 227, 233, 248, 256, 332
BaeI ACNNNNGTAYC 2 cut(s) 180, 213
BanI GGYRCC 5 cut(s) 182, 203, 245, 251, 329
BanII GRGCYC 1 cut(s) 533
BbsI GAAGAC 1 cut(s) 649
Bbv12I GWGCWC 2 cut(s) 342, 533
BbvI GCAGC 2 cut(s) 169, 534
BccI CCATC 5 cut(s) 41, 149, 548, 700, 747
BceAI ACGGC 1 cut(s) 422
BciT130I CCWGG 3 cut(s) 201, 348, 732
BcnI CCSGG 7 cut(s) 222, 243, 264, 265, 285, 306, 327
BcoDI GTCTC 2 cut(s) 215, 377
BfaI CTAG 1 cut(s) 534
BfmI CTRYAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 493
BisI GCNGC 4 cut(s) 88, 127, 158, 523
BlsI GCNGC 4 cut(s) 89, 128, 159, 524
BmeT110I CYCGRG 2 cut(s) 263, 526
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 1 cut(s) 650
BmiI GGNNCC 9 cut(s) 175, 184, 205, 247, 253, 331, 352, 358, 651
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 1 cut(s) 646
BmuI ACTGGG 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 649
BpmI CTGGAG 2 cut(s) 171, 423
BpuMI CCSGG 7 cut(s) 222, 243, 264, 265, 285, 306, 327
BsaBI GATNNNNATC 1 cut(s) 636
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 6 cut(s) 120, 199, 262, 263, 283, 305
BsaWI WCCGGW 9 cut(s) 164, 185, 206, 248, 332, 353, 389, 497, 640
BsaXI ACNNNNNCTCC 2 cut(s) 661, 691
Bse1I ACTGG 6 cut(s) 49, 62, 109, 154, 274, 454
Bse21I CCTNAGG 1 cut(s) 681
Bse8I GATNNNNATC 1 cut(s) 636
BseAI TCCGGA 1 cut(s) 640
BseBI CCWGG 3 cut(s) 201, 348, 732
BseDI CCNNGG 6 cut(s) 120, 199, 262, 263, 283, 305
BseGI GGATG 8 cut(s) 33, 141, 258, 279, 556, 637, 637, 739
BseJI GATNNNNATC 1 cut(s) 636
BseMII CTCAG 1 cut(s) 695
BseNI ACTGG 6 cut(s) 49, 62, 109, 154, 274, 454
BseRI GAGGAG 2 cut(s) 560, 669
BseSI GKGCMC 5 cut(s) 227, 233, 248, 256, 332
BseXI GCAGC 2 cut(s) 169, 534
Bsh1236I CGCG 1 cut(s) 15
Bsh1285I CGRYCG 1 cut(s) 390
BshFI GGCC 4 cut(s) 87, 129, 506, 652
BshNI GGYRCC 5 cut(s) 182, 203, 245, 251, 329
BshTI ACCGGT 7 cut(s) 164, 185, 206, 248, 332, 389, 497
BsiEI CGRYCG 1 cut(s) 390
BsiHKAI GWGCWC 2 cut(s) 342, 533
BsiHKCI CYCGRG 2 cut(s) 263, 526
BslFI GGGAC 4 cut(s) 182, 280, 322, 600
BsmAI GTCTC 2 cut(s) 215, 377
BsmBI CGTCTC 1 cut(s) 377
BsmFI GGGAC 4 cut(s) 182, 280, 322, 600
BsnI GGCC 4 cut(s) 87, 129, 506, 652
Bso31I GGTCTC 1 cut(s) 215
BsoBI CYCGRG 2 cut(s) 263, 526
Bsp1286I GDGCHC 7 cut(s) 227, 233, 248, 256, 332, 342, 533
Bsp13I TCCGGA 1 cut(s) 640
Bsp1407I TGTACA 1 cut(s) 316
Bsp143I GATC 2 cut(s) 570, 714
Bsp68I TCGCGA 1 cut(s) 15
BspACI CCGC 5 cut(s) 38, 88, 126, 618, 647
BspANI GGCC 4 cut(s) 87, 129, 506, 652
BspCNI CTCAG 1 cut(s) 694
BspEI TCCGGA 1 cut(s) 640
BspFNI CGCG 1 cut(s) 15
BspLI GGNNCC 9 cut(s) 175, 184, 205, 247, 253, 331, 352, 358, 651
BspPI GGATC 1 cut(s) 578
BspT107I GGYRCC 5 cut(s) 182, 203, 245, 251, 329
BspTNI GGTCTC 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 40
BsrGI TGTACA 1 cut(s) 316
BsrI ACTGG 6 cut(s) 49, 62, 109, 154, 274, 454
BssECI CCNNGG 6 cut(s) 120, 199, 262, 263, 283, 305
BssMI GATC 2 cut(s) 570, 714
Bst2UI CCWGG 3 cut(s) 201, 348, 732
Bst4CI ACNGT 2 cut(s) 466, 518
BstAPI GCANNNNNTGC 1 cut(s) 439
BstAUI TGTACA 1 cut(s) 316
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 1 cut(s) 681
BstEII GGTNACC 1 cut(s) 734
BstF5I GGATG 8 cut(s) 33, 141, 258, 279, 556, 637, 637, 739
BstFNI CGCG 1 cut(s) 15
BstH2I RGCGCY 1 cut(s) 493
BstHHI GCGC 1 cut(s) 492
BstKTI GATC 2 cut(s) 573, 717
BstMAI GTCTC 2 cut(s) 215, 377
BstMBI GATC 2 cut(s) 570, 714
BstMCI CGRYCG 1 cut(s) 390
BstMWI GCNNNNNNNGC 3 cut(s) 132, 439, 528
BstNI CCWGG 3 cut(s) 201, 348, 732
BstNSI RCATGY 3 cut(s) 323, 346, 437
BstPI GGTNACC 1 cut(s) 734
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 5 cut(s) 227, 233, 248, 256, 332
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 2 cut(s) 169, 534
BstV2I GAAGAC 1 cut(s) 649
Bsu36I CCTNAGG 1 cut(s) 681
BsuRI GGCC 4 cut(s) 87, 129, 506, 652
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 8 cut(s) 33, 141, 258, 279, 556, 637, 637, 739
BtsIMutI CAGTG 2 cut(s) 102, 745
BtuMI TCGCGA 1 cut(s) 15
Cac8I GCNNGC 1 cut(s) 435
CfoI GCGC 1 cut(s) 492
Cfr13I GGNCC 1 cut(s) 650
Cfr9I CCCGGG 1 cut(s) 263
CsiI ACCWGGT 1 cut(s) 730
Csp6I GTAC 4 cut(s) 147, 189, 317, 378
CspAI ACCGGT 7 cut(s) 164, 185, 206, 248, 332, 389, 497
CviAII CATG 8 cut(s) 94, 299, 320, 343, 434, 443, 689, 703
CviQI GTAC 4 cut(s) 147, 189, 317, 378
DdeI CTNAG 1 cut(s) 681
DpnI GATC 2 cut(s) 572, 716
DpnII GATC 2 cut(s) 570, 714
EaeI YGGCCR 1 cut(s) 127
Ecl136II GAGCTC 1 cut(s) 531
Eco24I GRGCYC 1 cut(s) 533
Eco31I GGTCTC 1 cut(s) 215
Eco53kI GAGCTC 1 cut(s) 531
Eco57I CTGAAG 1 cut(s) 611
Eco81I CCTNAGG 1 cut(s) 681
Eco88I CYCGRG 2 cut(s) 263, 526
Eco91I GGTNACC 1 cut(s) 734
EcoICRI GAGCTC 1 cut(s) 531
EcoO65I GGTNACC 1 cut(s) 734
EcoRII CCWGG 3 cut(s) 199, 346, 730
EcoT38I GRGCYC 1 cut(s) 533
Esp3I CGTCTC 1 cut(s) 377
FaeI CATG 8 cut(s) 97, 302, 323, 346, 437, 446, 692, 706
FalI AAGNNNNNCTT 2 cut(s) 596, 628
FaqI GGGAC 4 cut(s) 182, 280, 322, 600
FatI CATG 8 cut(s) 93, 298, 319, 342, 433, 442, 688, 702
FauI CCCGC 2 cut(s) 45, 640
Fnu4HI GCNGC 4 cut(s) 88, 127, 158, 523
FokI GGATG 8 cut(s) 20, 128, 245, 266, 563, 624, 644, 726
FriOI GRGCYC 1 cut(s) 533
Fsp4HI GCNGC 4 cut(s) 88, 127, 158, 523
FspBI CTAG 1 cut(s) 534
GlaI GCGC 1 cut(s) 491
GluI GCNGC 4 cut(s) 88, 127, 158, 523
GsuI CTGGAG 2 cut(s) 171, 423
HaeII RGCGCY 1 cut(s) 493
HaeIII GGCC 4 cut(s) 87, 129, 506, 652
HhaI GCGC 1 cut(s) 492
Hin1II CATG 8 cut(s) 97, 302, 323, 346, 437, 446, 692, 706
Hin6I GCGC 1 cut(s) 490
HinP1I GCGC 1 cut(s) 490
HinfI GANTC 1 cut(s) 676
HphI GGTGA 7 cut(s) 34, 347, 418, 482, 512, 521, 728
Hpy166II GTNNAC 5 cut(s) 191, 317, 473, 512, 623
Hpy188I TCNGA 2 cut(s) 173, 544
Hpy188III TCNNGA 5 cut(s) 14, 574, 641, 661, 683
Hpy8I GTNNAC 5 cut(s) 191, 317, 473, 512, 623
Hpy99I CGWCG 3 cut(s) 20, 522, 676
HpyAV CCTTC 3 cut(s) 403, 577, 586
HpyCH4III ACNGT 2 cut(s) 466, 518
HpyCH4IV ACGT 3 cut(s) 195, 216, 237
HpyCH4V TGCA 2 cut(s) 97, 442
HpyF10VI GCNNNNNNNGC 3 cut(s) 132, 439, 528
HpyF3I CTNAG 1 cut(s) 681
HpySE526I ACGT 3 cut(s) 195, 216, 237
Hsp92II CATG 8 cut(s) 97, 302, 323, 346, 437, 446, 692, 706
HspAI GCGC 1 cut(s) 490
Kpn2I TCCGGA 1 cut(s) 640
Kzo9I GATC 2 cut(s) 570, 714
LmnI GCTCC 3 cut(s) 356, 487, 685
Lsp1109I GCAGC 2 cut(s) 169, 534
LweI GCATC 1 cut(s) 646
MabI ACCWGGT 1 cut(s) 730
MaeI CTAG 1 cut(s) 534
MaeII ACGT 3 cut(s) 195, 216, 237
MaeIII GTNAC 2 cut(s) 726, 734
MalI GATC 2 cut(s) 572, 716
MbiI CCGCTC 1 cut(s) 40
MboI GATC 2 cut(s) 570, 714
MboII GAAGA 7 cut(s) 531, 589, 592, 610, 649, 706, 724
MhlI GDGCHC 7 cut(s) 227, 233, 248, 256, 332, 342, 533
MluCI AATT 1 cut(s) 9
MlyI GAGTC 1 cut(s) 670
MroI TCCGGA 1 cut(s) 640
MslI CAYNNNNRTG 2 cut(s) 498, 630
MspA1I CMGCKG 1 cut(s) 70
MvaI CCWGG 3 cut(s) 201, 348, 732
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 3 cut(s) 132, 439, 528
NciI CCSGG 7 cut(s) 222, 243, 264, 265, 285, 306, 327
NdeII GATC 2 cut(s) 570, 714
NlaIII CATG 8 cut(s) 97, 302, 323, 346, 437, 446, 692, 706
NlaIV GGNNCC 9 cut(s) 175, 184, 205, 247, 253, 331, 352, 358, 651
NmuCI GTSAC 1 cut(s) 734
NruI TCGCGA 1 cut(s) 15
NspI RCATGY 3 cut(s) 323, 346, 437
OliI CACNNNNGTG 1 cut(s) 498
PaeI GCATGC 1 cut(s) 437
PaeR7I CTCGAG 1 cut(s) 526
PciI ACATGT 1 cut(s) 319
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PinAI ACCGGT 7 cut(s) 164, 185, 206, 248, 332, 389, 497
PkrI GCNGC 4 cut(s) 89, 128, 159, 524
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
PscI ACATGT 1 cut(s) 319
Psp124BI GAGCTC 1 cut(s) 533
Psp6I CCWGG 3 cut(s) 199, 346, 730
PspEI GGTNACC 1 cut(s) 734
PspGI CCWGG 3 cut(s) 199, 346, 730
PspN4I GGNNCC 9 cut(s) 175, 184, 205, 247, 253, 331, 352, 358, 651
PspPI GGNCC 1 cut(s) 650
PspXI VCTCGAGB 1 cut(s) 526
PvuII CAGCTG 1 cut(s) 70
RruI TCGCGA 1 cut(s) 15
RsaI GTAC 4 cut(s) 148, 190, 318, 379
RsaNI GTAC 4 cut(s) 147, 189, 317, 378
RseI CAYNNNNRTG 2 cut(s) 498, 630
SacI GAGCTC 1 cut(s) 533
SatI GCNGC 4 cut(s) 88, 127, 158, 523
Sau3AI GATC 2 cut(s) 570, 714
Sau96I GGNCC 1 cut(s) 650
SchI GAGTC 1 cut(s) 670
SduI GDGCHC 7 cut(s) 227, 233, 248, 256, 332, 342, 533
SexAI ACCWGGT 1 cut(s) 730
SfaNI GCATC 1 cut(s) 646
SfcI CTRYAG 1 cut(s) 465
Sfr274I CTCGAG 1 cut(s) 526
SgrAI CRCCGGYG 3 cut(s) 248, 332, 497
SlaI CTCGAG 1 cut(s) 526
SmaI CCCGGG 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 498, 630
SmlI CTYRAG 1 cut(s) 526
SmoI CTYRAG 1 cut(s) 526
SphI GCATGC 1 cut(s) 437
Sse9I AATT 1 cut(s) 9
SsiI CCGC 5 cut(s) 38, 88, 126, 618, 647
SspMI CTAG 1 cut(s) 534
SstI GAGCTC 1 cut(s) 533
TaaI ACNGT 2 cut(s) 466, 518
TaiI ACGT 3 cut(s) 198, 219, 240
TaqI TCGA 1 cut(s) 527
TasI AATT 1 cut(s) 9
TatI WGTACW 1 cut(s) 316
TauI GCSGC 2 cut(s) 90, 129
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 1 cut(s) 734
TseI GCWGC 2 cut(s) 157, 522
Tsp45I GTSAC 1 cut(s) 734
TspGWI ACGGA 2 cut(s) 134, 395
TspMI CCCGGG 1 cut(s) 263
TspRI CASTG 1 cut(s) 109
XapI RAATTY 1 cut(s) 9
XceI RCATGY 3 cut(s) 323, 346, 437
XhoI CTCGAG 1 cut(s) 526
XmaI CCCGGG 1 cut(s) 263
XspI CTAG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.