RLG00000014246

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004125
Physical Location & Seq
Forward (+)
49887230 .. 49891611
4382 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014246

Sequence Viewer

Length: 636 bp
ATGCTTGCCTGGCTTGCAAGAGAGCTTCAAGCAATAGACCATTCGCCACACCGGTCTCATCAAATTGATGTTGAGAGACATGCCAGAGATTTTTTGAAAGCTGCCAAAAAGCTTCAGCTCTTTTTTGTTGGTCTGCAACGTGAGGATCTGCCCACGGATGCTGAAAAACTCAGAAAGAAGATTGCTGTGATGGAAGAGGAGTTGAAGGTAAAGAATGAGATTATTAAGAAGAATGAAAGGCGGCCTCTGAATCAAAATATAAAGACCCCAACCAATTTCTTCACCAAGTTACAAATTTCACTTACTCTCCTTGTGGATTCTCCGGTGTTGGTGAAGATGGAATCGCAGAAAAGAGAACTTGAAAAACAGCCTGCTGGAACAACTGCTACTCCTGCTGCTGCTGCTGCAACCCCCACATCTTGCCGAAAGAAGAAGAATGAACAAGCCACTTTCTTGGAAGATGTGAAGGATCACATTGATGAGTTTATCCACGCATCTATGGACGAACACGTGTCTTGCTTCAAAAAGACCATTGACAAGATGTTTAAAATGTCAAAGGTTGTTGCTGAGAAGAACGCTGCTGACGACAAGGGAGTTGAGAGTTCTCTTCCCCTTCGAACAACAGTAGCAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

24.01

Weight (kDa)

9.1

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med28 PF11594 11 - 80 2.6e-11 Mediator complex subunit 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 241
AclWI GGATC 2 cut(s) 153, 477
AcsI RAATTY 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 98
AcvI CACGTG 1 cut(s) 511
AflIII ACRYGT 2 cut(s) 508, 510
AgeI ACCGGT 1 cut(s) 51
AgsI TTSAA 5 cut(s) 29, 97, 205, 362, 523
AjnI CCWGG 1 cut(s) 8
AluBI AGCT 4 cut(s) 25, 101, 112, 118
AluI AGCT 4 cut(s) 25, 101, 112, 118
Alw26I GTCTC 2 cut(s) 60, 70
AlwI GGATC 2 cut(s) 153, 477
AoxI GGCC 1 cut(s) 242
ApeKI GCWGC 6 cut(s) 101, 395, 398, 401, 404, 578
ApoI RAATTY 1 cut(s) 294
AsiGI ACCGGT 1 cut(s) 51
AsuHPI GGTGA 2 cut(s) 274, 343
AsuII TTCGAA 1 cut(s) 616
BbrPI CACGTG 1 cut(s) 511
BbvI GCAGC 6 cut(s) 88, 382, 385, 388, 391, 565
BccI CCATC 2 cut(s) 184, 331
BciT130I CCWGG 1 cut(s) 10
BcoDI GTCTC 2 cut(s) 60, 70
BisI GCNGC 7 cut(s) 102, 242, 396, 399, 402, 405, 579
BlsI GCNGC 7 cut(s) 103, 243, 397, 400, 403, 406, 580
Bme1390I CCNGG 1 cut(s) 10
BmrFI CCNGG 1 cut(s) 10
BmsI GCATC 2 cut(s) 148, 503
Bpu14I TTCGAA 1 cut(s) 616
BsaAI YACGTR 1 cut(s) 511
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 153
BsaWI WCCGGW 2 cut(s) 51, 322
BsaXI ACNNNNNCTCC 4 cut(s) 291, 321, 373, 403
Bse118I RCCGGY 1 cut(s) 51
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 163
BseMII CTCAG 2 cut(s) 184, 558
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 6 cut(s) 88, 382, 385, 388, 391, 565
BshFI GGCC 1 cut(s) 244
BshTI ACCGGT 1 cut(s) 51
BsiSI CCGG 2 cut(s) 52, 323
BsmAI GTCTC 2 cut(s) 60, 70
BsnI GGCC 1 cut(s) 244
Bso31I GGTCTC 1 cut(s) 60
Bsp119I TTCGAA 1 cut(s) 616
Bsp143I GATC 2 cut(s) 145, 469
BspACI CCGC 1 cut(s) 241
BspANI GGCC 1 cut(s) 244
BspCNI CTCAG 2 cut(s) 183, 559
BspPI GGATC 2 cut(s) 153, 477
BspT104I TTCGAA 1 cut(s) 616
BspTNI GGTCTC 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 51
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 2 cut(s) 145, 469
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 1 cut(s) 625
Bst6I CTCTTC 2 cut(s) 189, 612
BstBAI YACGTR 1 cut(s) 511
BstBI TTCGAA 1 cut(s) 616
BstC8I GCNNGC 3 cut(s) 6, 15, 372
BstDEI CTNAG 2 cut(s) 170, 567
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 163
BstKTI GATC 2 cut(s) 148, 472
BstMAI GTCTC 2 cut(s) 60, 70
BstMBI GATC 2 cut(s) 145, 469
BstMWI GCNNNNNNNGC 5 cut(s) 10, 14, 392, 401, 404
BstNI CCWGG 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 8
BstV1I GCAGC 6 cut(s) 88, 382, 385, 388, 391, 565
BstX2I RGATCY 1 cut(s) 145
BstXI CCANNNNNNTGG 1 cut(s) 454
BstYI RGATCY 1 cut(s) 145
BsuRI GGCC 1 cut(s) 244
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 163
Cac8I GCNNGC 3 cut(s) 6, 15, 372
Cfr10I RCCGGY 1 cut(s) 51
CspAI ACCGGT 1 cut(s) 51
CviAII CATG 1 cut(s) 80
CviJI RGCY 8 cut(s) 13, 25, 101, 112, 118, 244, 370, 446
CviKI_1 RGCY 8 cut(s) 13, 25, 101, 112, 118, 244, 370, 446
DdeI CTNAG 2 cut(s) 170, 567
DpnI GATC 2 cut(s) 147, 471
DpnII GATC 2 cut(s) 145, 469
DraI TTTAAA 1 cut(s) 547
Eam1104I CTCTTC 2 cut(s) 189, 612
EarI CTCTTC 2 cut(s) 189, 612
Eco31I GGTCTC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 98
Eco72I CACGTG 1 cut(s) 511
EcoRII CCWGG 1 cut(s) 8
FaeI CATG 1 cut(s) 83
FaiI YATR 3 cut(s) 81, 260, 500
FatI CATG 1 cut(s) 79
Fnu4HI GCNGC 7 cut(s) 102, 242, 396, 399, 402, 405, 579
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 7 cut(s) 102, 242, 396, 399, 402, 405, 579
GluI GCNGC 7 cut(s) 102, 242, 396, 399, 402, 405, 579
HaeIII GGCC 1 cut(s) 244
HapII CCGG 2 cut(s) 52, 323
Hin1II CATG 1 cut(s) 83
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 3 cut(s) 250, 317, 341
HpaII CCGG 2 cut(s) 52, 323
HphI GGTGA 2 cut(s) 274, 343
Hpy188I TCNGA 2 cut(s) 173, 249
HpyAV CCTTC 3 cut(s) 199, 460, 623
HpyCH4III ACNGT 1 cut(s) 625
HpyCH4IV ACGT 2 cut(s) 139, 510
HpyCH4V TGCA 3 cut(s) 17, 136, 407
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 14, 392, 401, 404
HpyF3I CTNAG 2 cut(s) 170, 567
HpySE526I ACGT 2 cut(s) 139, 510
Hsp92II CATG 1 cut(s) 83
Kzo9I GATC 2 cut(s) 145, 469
LpnPI CCDG 7 cut(s) 22, 65, 97, 336, 360, 384, 405
Lsp1109I GCAGC 6 cut(s) 88, 382, 385, 388, 391, 565
LweI GCATC 2 cut(s) 148, 503
MaeII ACGT 2 cut(s) 139, 510
MaeIII GTNAC 1 cut(s) 288
MalI GATC 2 cut(s) 147, 471
MboI GATC 2 cut(s) 145, 469
MflI RGATCY 1 cut(s) 145
MluCI AATT 3 cut(s) 63, 274, 294
MnlI CCTC 3 cut(s) 136, 190, 255
MseI TTAA 2 cut(s) 225, 546
MslI CAYNNNNRTG 1 cut(s) 477
MspI CCGG 2 cut(s) 52, 323
MspR9I CCNGG 1 cut(s) 10
MvaI CCWGG 1 cut(s) 10
MwoI GCNNNNNNNGC 5 cut(s) 10, 14, 392, 401, 404
NdeII GATC 2 cut(s) 145, 469
NlaIII CATG 1 cut(s) 83
NspI RCATGY 1 cut(s) 83
NspV TTCGAA 1 cut(s) 616
PcsI WCGNNNNNNNCGW 1 cut(s) 582
PfeI GAWTC 3 cut(s) 250, 317, 341
PinAI ACCGGT 1 cut(s) 51
PkrI GCNGC 7 cut(s) 103, 243, 397, 400, 403, 406, 580
PmaCI CACGTG 1 cut(s) 511
PmlI CACGTG 1 cut(s) 511
Ppu21I YACGTR 1 cut(s) 511
Psp6I CCWGG 1 cut(s) 8
PspCI CACGTG 1 cut(s) 511
PspGI CCWGG 1 cut(s) 8
PsrI GAACNNNNNNTAC 2 cut(s) 370, 402
PsuI RGATCY 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 477
SaqAI TTAA 2 cut(s) 225, 546
SatI GCNGC 7 cut(s) 102, 242, 396, 399, 402, 405, 579
Sau3AI GATC 2 cut(s) 145, 469
ScrFI CCNGG 1 cut(s) 10
SetI ASST 8 cut(s) 27, 103, 114, 120, 142, 210, 513, 561
SfaNI GCATC 2 cut(s) 148, 503
SfuI TTCGAA 1 cut(s) 616
SmiMI CAYNNNNRTG 1 cut(s) 477
Sse9I AATT 3 cut(s) 63, 274, 294
SsiI CCGC 1 cut(s) 241
StyD4I CCNGG 1 cut(s) 8
TaaI ACNGT 1 cut(s) 625
TaiI ACGT 2 cut(s) 142, 513
TaqI TCGA 1 cut(s) 616
TasI AATT 3 cut(s) 63, 274, 294
TauI GCSGC 1 cut(s) 244
TfiI GAWTC 3 cut(s) 250, 317, 341
Tru1I TTAA 2 cut(s) 225, 546
Tru9I TTAA 2 cut(s) 225, 546
TseI GCWGC 6 cut(s) 101, 395, 398, 401, 404, 578
TspDTI ATGAA 2 cut(s) 249, 453
TspGWI ACGGA 1 cut(s) 170
XapI RAATTY 1 cut(s) 294
XceI RCATGY 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.