RLG00000014352

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005291
Physical Location & Seq
Forward (+)
51907162 .. 51910630
3469 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014352

Sequence Viewer

Length: 1752 bp
ATGAGTGACCACAATTCAACCAGCCCAGATTTTAGGGTTTCTCCTTCCACAATGGCGACAACGAAGCCTCAGATGATGGCGGCCCAGGACTTGACAATAGAGAAGCCGGCGATGATGGCCGCGAAGCCGCCGAGGAGGCTGTTGCAGTGGCCGGAGCAATCTCTGAAAGAGGCTCTCATCTCTCACGTTCAATCCATAAAATCTGGTTTAACACTGGGCATTTTCACCTCCAACCAACTCATTCACCATTACTCCAGACATGGCCTCATTCAAGACGCCCAGAAGCTGTTCGACGAAATGCCCCACCGAAACGTCTACTCTTGGAACGCCATAATCTCCACCCACATCAAATCACGCAACTTGGAACGTGCCCGGCACCTATTCGACGCTGCTTCTTTCAAAGATTTGGTCACTTACAACTCCATGCTCTCTGGCTACGCAAGCACAGAAGGTTACGAGGCTTGTGCGCTTGGGTTGTGTGCCGAAATGCAGTCGCGGGATGATCGGATTAGAGTTGATGAGGTTACTCTAACTACCATGCTTAACTTGTGTGCCAAGTTAGAGGTGGTTTGTTATGGAAAGCAGTTGCATTCCTGTATGGTGAGAACTGCCAATGATCTGAGTGGGTTTGCGGTGAGCTCTTTAATTGATATGTACTCTAAGTGCGGGTGTTTTCGTGATGCTTGGCGCGTGTTTAGTGGATATAGAGGGATGGTTGACTTGGTTTCGAAGAATGCAATGGTGGCAGCTTGTTGTAGAGAGCAAAATTGGATATGGCGGTTGATCTTTTTTTGGAGAGAACCGGAGATTAACGATACTGTGTCTTGGAACACAATGATTTCAGGGTATGTGCAGAATGGTTTTGTAGAGGAGGCGCTGAATTTGTTTGTTCGCATGGCGGAGAATGGATTTCGATGGAATGAGCATACTTTTGCTAGTGTTTTGAGTGCTTGCTCTGGTTTGAGGAACTTCAAACTAGGAAAGGAAGTCCATAGTTGGGTGTTGAAGAATGGGATGAGTTCTAATCCGTTTATAAGTAGTGGCATTGTTGATGTCTATTGTAAGTGTGGAAATATGAGTTATGCAAAGTCAGTTCATGCAGCAATGGGAATTAGAAATTCTTTTTCAATCACTTCATTGATCTTGGGTCATGCCTCTCAAGCTTTGTTTTCTGGATATCTCAAATCACAACAATTTGAAGCTGTATTTAAGCTTTTGAGCGAGTTCAGGGAAAAGGAAGTAATTGTTCCTGATGCTTTGATTCTCATTAGTGTGCTAGGTGCCTGTGCAATTCAAGCTGCCCTGGACCCTGGGACGCAGATTCATGCTTACATCTTGAGGAATAACATTGAAGGAGACAAGAAATTGTTCAGTGCTTTGATTGATATGTACTCAAAATCTGGGAATATTACTTATGCGGAAAAGATTTTTAAAAGCGTTTTTGACAGGGATATAATCGTTTACAATGTGATATTAGCTGGTTATGCTCACCATGGTTGTGAAACTAAAGCCATCCAAATCTTCAAAGAACTGTTGAAGAGAGGTATTAGGCCCGATGCAGTCACATTTTTAGCACTTCTTTCAGCTTGTCGTCACTCTTGTTTGGTGGAATTGGGGGAGCAATTTTTCTGGTCCATGACAAAAGATTATAACCTATTGCCTGAACTGGATCACTATGCATGTGTGATTGACTTGTATGGGAGGGCTAATCAACTTGAGAAGGCAAAAGCATTGATGGAAATATTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PfkB TB2_DP1_HVA22

Protein Analysis

584

Amino Acids

65.7

Weight (kDa)

8.04

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 272 - 318 6.2e-12 PPR repeat family
PPR_1 PF12854 272 - 299 1e-05 PPR repeat
PPR PF01535 274 - 304 3.3e-10 PPR repeat
PPR_2 PF13041 484 - 530 1.9e-10 PPR repeat family
PPR_3 PF13812 484 - 530 2.3e-06 Pentatricopeptide repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1034, 1650
AccB1I GGYRCC 2 cut(s) 375, 1280
AccI GTMKAC 1 cut(s) 315
AccII CGCG 3 cut(s) 122, 496, 690
AciI CCGC 9 cut(s) 80, 120, 128, 496, 632, 666, 778, 899, 1418
AclWI GGATC 1 cut(s) 1677
AcoI YGGCCR 2 cut(s) 117, 149
AcsI RAATTY 2 cut(s) 880, 1117
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 2 cut(s) 656, 1391
AfiI CCNNNNNNNGG 1 cut(s) 997
AjnI CCWGG 3 cut(s) 84, 1302, 1309
AluBI AGCT 9 cut(s) 286, 639, 749, 1163, 1202, 1213, 1298, 1478, 1586
AluI AGCT 9 cut(s) 286, 639, 749, 1163, 1202, 1213, 1298, 1478, 1586
Alw21I GWGCWC 1 cut(s) 641
Alw26I GTCTC 1 cut(s) 1350
AlwI GGATC 1 cut(s) 1677
AlwNI CAGNNNCTG 1 cut(s) 286
AoxI GGCC 5 cut(s) 81, 117, 149, 262, 1550
ApeKI GCWGC 4 cut(s) 389, 746, 1100, 1298
ApoI RAATTY 2 cut(s) 880, 1117
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
Asp700I GAANNNNTTC 2 cut(s) 287, 1367
AspLEI GCGC 3 cut(s) 469, 690, 877
AspS9I GGNCC 4 cut(s) 82, 1306, 1551, 1632
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 5 cut(s) 217, 236, 613, 646, 1481
AsuII TTCGAA 1 cut(s) 728
AvaII GGWCC 2 cut(s) 1306, 1632
BaeGI GKGCMC 1 cut(s) 373
BanI GGYRCC 2 cut(s) 375, 1280
BanII GRGCYC 1 cut(s) 641
Bbv12I GWGCWC 1 cut(s) 641
BbvI GCAGC 4 cut(s) 376, 758, 1112, 1285
BccI CCATC 6 cut(s) 70, 109, 706, 909, 1520, 1729
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BciT130I CCWGG 3 cut(s) 86, 1304, 1311
BcnI CCSGG 1 cut(s) 373
BcoDI GTCTC 1 cut(s) 1350
BfaI CTAG 3 cut(s) 936, 977, 1277
BfoI RGCGCY 1 cut(s) 878
BglI GCCNNNNNGGC 1 cut(s) 136
BisI GCNGC 7 cut(s) 81, 120, 128, 390, 747, 1101, 1299
BlsI GCNGC 7 cut(s) 82, 121, 129, 391, 748, 1102, 1300
Bme1390I CCNGG 4 cut(s) 86, 373, 1304, 1311
Bme18I GGWCC 2 cut(s) 1306, 1632
BmgT120I GGNCC 4 cut(s) 82, 1306, 1551, 1632
BmiI GGNNCC 3 cut(s) 377, 1282, 1308
BmrFI CCNGG 4 cut(s) 86, 373, 1304, 1311
BmrI ACTGGG 1 cut(s) 224
BmsI GCATC 3 cut(s) 670, 1243, 1546
BmuI ACTGGG 1 cut(s) 224
BpmI CTGGAG 1 cut(s) 238
Bpu14I TTCGAA 1 cut(s) 728
BpuEI CTTGAG 3 cut(s) 1143, 1357, 1736
BpuMI CCSGG 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 6 cut(s) 84, 131, 1302, 1309, 1310, 1492
BsaWI WCCGGW 1 cut(s) 802
BsaXI ACNNNNNCTCC 2 cut(s) 236, 266
Bsc4I CCNNNNNNNGG 1 cut(s) 997
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 2 cut(s) 219, 1671
Bse3DI GCAATG 2 cut(s) 744, 1110
BseBI CCWGG 3 cut(s) 86, 1304, 1311
BseDI CCNNGG 6 cut(s) 84, 131, 1302, 1309, 1310, 1492
BseGI GGATG 4 cut(s) 505, 717, 1020, 1512
BseLI CCNNNNNNNGG 1 cut(s) 997
BseMI GCAATG 2 cut(s) 744, 1110
BseMII CTCAG 2 cut(s) 83, 611
BseNI ACTGG 2 cut(s) 219, 1671
BseRI GAGGAG 2 cut(s) 148, 884
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 4 cut(s) 376, 758, 1112, 1285
BsgI GTGCAG 1 cut(s) 872
Bsh1236I CGCG 3 cut(s) 122, 496, 690
BshFI GGCC 5 cut(s) 83, 119, 151, 264, 1552
BshNI GGYRCC 2 cut(s) 375, 1280
BsiHKAI GWGCWC 1 cut(s) 641
BsiSI CCGG 4 cut(s) 107, 152, 373, 803
BslFI GGGAC 1 cut(s) 1327
BslI CCNNNNNNNGG 1 cut(s) 997
BsmAI GTCTC 1 cut(s) 1350
BsmFI GGGAC 1 cut(s) 1327
BsmI GAATGC 2 cut(s) 589, 739
BsnI GGCC 5 cut(s) 83, 119, 151, 264, 1552
Bsp119I TTCGAA 1 cut(s) 728
Bsp1286I GDGCHC 2 cut(s) 373, 641
Bsp143I GATC 5 cut(s) 502, 616, 783, 1140, 1669
Bsp19I CCATGG 1 cut(s) 1492
BspACI CCGC 9 cut(s) 80, 120, 128, 496, 632, 666, 778, 899, 1418
BspANI GGCC 5 cut(s) 83, 119, 151, 264, 1552
BspCNI CTCAG 2 cut(s) 82, 612
BspFNI CGCG 3 cut(s) 122, 496, 690
BspLI GGNNCC 3 cut(s) 377, 1282, 1308
BspPI GGATC 1 cut(s) 1677
BspT104I TTCGAA 1 cut(s) 728
BspT107I GGYRCC 2 cut(s) 375, 1280
BsrDI GCAATG 2 cut(s) 744, 1110
BsrFI RCCGGY 1 cut(s) 106
BsrI ACTGG 2 cut(s) 219, 1671
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 6 cut(s) 84, 131, 1302, 1309, 1310, 1492
BssMI GATC 5 cut(s) 502, 616, 783, 1140, 1669
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 1492
Bst2UI CCWGG 3 cut(s) 86, 1304, 1311
Bst4CI ACNGT 2 cut(s) 820, 1533
Bst6I CTCTTC 1 cut(s) 1532
BstACI GRCGYC 1 cut(s) 276
BstBI TTCGAA 1 cut(s) 728
BstC8I GCNNGC 3 cut(s) 108, 442, 952
BstDEI CTNAG 3 cut(s) 69, 620, 660
BstDSI CCRYGG 1 cut(s) 1492
BstF5I GGATG 4 cut(s) 505, 717, 1020, 1512
BstFNI CGCG 3 cut(s) 122, 496, 690
BstH2I RGCGCY 1 cut(s) 878
BstHHI GCGC 3 cut(s) 469, 690, 877
BstKTI GATC 5 cut(s) 505, 619, 786, 1143, 1672
BstMAI GTCTC 1 cut(s) 1350
BstMBI GATC 5 cut(s) 502, 616, 783, 1140, 1669
BstMWI GCNNNNNNNGC 7 cut(s) 116, 136, 441, 743, 1160, 1295, 1484
BstNI CCWGG 3 cut(s) 86, 1304, 1311
BstNSI RCATGY 1 cut(s) 1683
BstSCI CCNGG 4 cut(s) 84, 371, 1302, 1309
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 3 cut(s) 122, 496, 690
BstV1I GCAGC 4 cut(s) 376, 758, 1112, 1285
BsuRI GGCC 5 cut(s) 83, 119, 151, 264, 1552
BtgI CCRYGG 1 cut(s) 1492
BtgZI GCGATG 1 cut(s) 125
BtsCI GGATG 4 cut(s) 505, 717, 1020, 1512
BtsI GCAGTG 1 cut(s) 152
BtsIMutI CAGTG 3 cut(s) 152, 212, 1378
Cac8I GCNNGC 3 cut(s) 108, 442, 952
CaiI CAGNNNCTG 1 cut(s) 286
CfoI GCGC 3 cut(s) 469, 690, 877
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 4 cut(s) 82, 1306, 1551, 1632
CseI GACGC 3 cut(s) 284, 395, 1324
Csp6I GTAC 2 cut(s) 655, 1390
CviQI GTAC 2 cut(s) 655, 1390
DdeI CTNAG 3 cut(s) 69, 620, 660
DpnI GATC 5 cut(s) 504, 618, 785, 1142, 1671
DpnII GATC 5 cut(s) 502, 616, 783, 1140, 1669
DraI TTTAAA 1 cut(s) 1432
EaeI YGGCCR 2 cut(s) 117, 149
Eam1104I CTCTTC 1 cut(s) 1532
EarI CTCTTC 1 cut(s) 1532
EciI GGCGGA 1 cut(s) 914
Ecl136II GAGCTC 1 cut(s) 639
Eco130I CCWWGG 1 cut(s) 1492
Eco24I GRGCYC 1 cut(s) 641
Eco32I GATATC 1 cut(s) 1178
Eco47I GGWCC 2 cut(s) 1306, 1632
Eco53kI GAGCTC 1 cut(s) 639
EcoICRI GAGCTC 1 cut(s) 639
EcoRII CCWGG 3 cut(s) 84, 1302, 1309
EcoRV GATATC 1 cut(s) 1178
EcoT14I CCWWGG 1 cut(s) 1492
EcoT22I ATGCAT 1 cut(s) 1681
EcoT38I GRGCYC 1 cut(s) 641
ErhI CCWWGG 1 cut(s) 1492
FaqI GGGAC 1 cut(s) 1327
FauI CCCGC 2 cut(s) 489, 659
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 7 cut(s) 81, 120, 128, 390, 747, 1101, 1299
FokI GGATG 4 cut(s) 512, 724, 1027, 1499
FriOI GRGCYC 1 cut(s) 641
Fsp4HI GCNGC 7 cut(s) 81, 120, 128, 390, 747, 1101, 1299
FspBI CTAG 3 cut(s) 936, 977, 1277
GlaI GCGC 3 cut(s) 468, 689, 876
GluI GCNGC 7 cut(s) 81, 120, 128, 390, 747, 1101, 1299
GsuI CTGGAG 1 cut(s) 238
HaeII RGCGCY 1 cut(s) 878
HaeIII GGCC 5 cut(s) 83, 119, 151, 264, 1552
HapII CCGG 4 cut(s) 107, 152, 373, 803
HgaI GACGC 3 cut(s) 284, 395, 1324
HhaI GCGC 3 cut(s) 469, 690, 877
Hin1I GRCGYC 1 cut(s) 276
Hin6I GCGC 3 cut(s) 467, 688, 875
HinP1I GCGC 3 cut(s) 467, 688, 875
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HindIII AAGCTT 2 cut(s) 1161, 1211
HinfI GANTC 2 cut(s) 1261, 1321
HpaII CCGG 4 cut(s) 107, 152, 373, 803
HphI GGTGA 5 cut(s) 217, 236, 613, 646, 1481
Hpy166II GTNNAC 3 cut(s) 316, 718, 1462
Hpy188I TCNGA 4 cut(s) 72, 165, 507, 621
Hpy188III TCNNGA 6 cut(s) 255, 272, 677, 1173, 1250, 1336
Hpy8I GTNNAC 3 cut(s) 316, 718, 1462
Hpy99I CGWCG 2 cut(s) 296, 389
HpyAV CCTTC 4 cut(s) 54, 443, 1346, 1714
HpyCH4III ACNGT 2 cut(s) 820, 1533
HpyCH4IV ACGT 3 cut(s) 186, 312, 367
HpyF10VI GCNNNNNNNGC 7 cut(s) 116, 136, 441, 743, 1160, 1295, 1484
HpyF3I CTNAG 3 cut(s) 69, 620, 660
HpySE526I ACGT 3 cut(s) 186, 312, 367
Hsp92I GRCGYC 1 cut(s) 276
HspAI GCGC 3 cut(s) 467, 688, 875
KroI GCCGGC 1 cut(s) 106
KroNI GCCGGC 1 cut(s) 108
Kzo9I GATC 5 cut(s) 502, 616, 783, 1140, 1669
LmnI GCTCC 2 cut(s) 154, 1618
Lsp1109I GCAGC 4 cut(s) 376, 758, 1112, 1285
LweI GCATC 3 cut(s) 670, 1243, 1546
MaeI CTAG 3 cut(s) 936, 977, 1277
MaeII ACGT 3 cut(s) 186, 312, 367
MaeIII GTNAC 6 cut(s) 5, 409, 452, 523, 1561, 1592
MalI GATC 5 cut(s) 504, 618, 785, 1142, 1671
MboI GATC 5 cut(s) 502, 616, 783, 1140, 1669
MboII GAAGA 4 cut(s) 742, 1018, 1513, 1549
MhlI GDGCHC 2 cut(s) 373, 641
MmeI TCCRAC 1 cut(s) 255
Mph1103I ATGCAT 1 cut(s) 1681
MroNI GCCGGC 1 cut(s) 106
MroXI GAANNNNTTC 2 cut(s) 287, 1367
MseI TTAA 6 cut(s) 209, 543, 644, 810, 1209, 1431
MslI CAYNNNNRTG 2 cut(s) 1271, 1497
MspI CCGG 4 cut(s) 107, 152, 373, 803
MspR9I CCNGG 4 cut(s) 86, 373, 1304, 1311
Mva1269I GAATGC 2 cut(s) 589, 739
MvaI CCWGG 3 cut(s) 86, 1304, 1311
MvnI CGCG 3 cut(s) 122, 496, 690
MwoI GCNNNNNNNGC 7 cut(s) 116, 136, 441, 743, 1160, 1295, 1484
NaeI GCCGGC 1 cut(s) 108
NciI CCSGG 1 cut(s) 373
NcoI CCATGG 1 cut(s) 1492
NdeII GATC 5 cut(s) 502, 616, 783, 1140, 1669
NgoMIV GCCGGC 1 cut(s) 106
NlaIV GGNNCC 3 cut(s) 377, 1282, 1308
NmeAIII GCCGAG 1 cut(s) 156
NmuCI GTSAC 4 cut(s) 5, 409, 1561, 1592
NsiI ATGCAT 1 cut(s) 1681
NspI RCATGY 1 cut(s) 1683
NspV TTCGAA 1 cut(s) 728
PasI CCCWGGG 1 cut(s) 1310
PctI GAATGC 2 cut(s) 589, 739
PdiI GCCGGC 1 cut(s) 108
PdmI GAANNNNTTC 2 cut(s) 287, 1367
PfeI GAWTC 2 cut(s) 1261, 1321
PkrI GCNGC 7 cut(s) 82, 121, 129, 391, 748, 1102, 1300
PsiI TTATAA 2 cut(s) 1034, 1650
Psp124BI GAGCTC 1 cut(s) 641
Psp6I CCWGG 3 cut(s) 84, 1302, 1309
PspGI CCWGG 3 cut(s) 84, 1302, 1309
PspN4I GGNNCC 3 cut(s) 377, 1282, 1308
PspPI GGNCC 4 cut(s) 82, 1306, 1551, 1632
PstNI CAGNNNCTG 1 cut(s) 286
RsaI GTAC 2 cut(s) 656, 1391
RsaNI GTAC 2 cut(s) 655, 1390
RseI CAYNNNNRTG 2 cut(s) 1271, 1497
SacI GAGCTC 1 cut(s) 641
SaqAI TTAA 6 cut(s) 209, 543, 644, 810, 1209, 1431
SatI GCNGC 7 cut(s) 81, 120, 128, 390, 747, 1101, 1299
Sau3AI GATC 5 cut(s) 502, 616, 783, 1140, 1669
Sau96I GGNCC 4 cut(s) 82, 1306, 1551, 1632
ScrFI CCNGG 4 cut(s) 86, 373, 1304, 1311
SduI GDGCHC 2 cut(s) 373, 641
SfaNI GCATC 3 cut(s) 670, 1243, 1546
SfuI TTCGAA 1 cut(s) 728
SinI GGWCC 2 cut(s) 1306, 1632
SmiMI CAYNNNNRTG 2 cut(s) 1271, 1497
SmlI CTYRAG 3 cut(s) 1158, 1336, 1715
SmoI CTYRAG 3 cut(s) 1158, 1336, 1715
SsiI CCGC 9 cut(s) 80, 120, 128, 496, 632, 666, 778, 899, 1418
SspI AATATT 2 cut(s) 1408, 1743
SspMI CTAG 3 cut(s) 936, 977, 1277
SstI GAGCTC 1 cut(s) 641
StyD4I CCNGG 4 cut(s) 84, 371, 1302, 1309
StyI CCWWGG 1 cut(s) 1492
TaaI ACNGT 2 cut(s) 820, 1533
TaiI ACGT 3 cut(s) 189, 315, 370
TaqI TCGA 4 cut(s) 291, 384, 728, 913
TatI WGTACW 2 cut(s) 654, 1389
TauI GCSGC 3 cut(s) 83, 122, 130
TfiI GAWTC 2 cut(s) 1261, 1321
Tru1I TTAA 6 cut(s) 209, 543, 644, 810, 1209, 1431
Tru9I TTAA 6 cut(s) 209, 543, 644, 810, 1209, 1431
TscAI CASTG 3 cut(s) 152, 219, 1378
TseFI GTSAC 4 cut(s) 5, 409, 1561, 1592
TseI GCWGC 4 cut(s) 389, 746, 1100, 1298
Tsp45I GTSAC 4 cut(s) 5, 409, 1561, 1592
TspDTI ATGAA 3 cut(s) 1085, 1125, 1313
TspGWI ACGGA 1 cut(s) 1017
TspRI CASTG 3 cut(s) 152, 219, 1378
VpaK11BI GGWCC 2 cut(s) 1306, 1632
XapI RAATTY 2 cut(s) 880, 1117
XceI RCATGY 1 cut(s) 1683
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 2 cut(s) 287, 1367
XspI CTAG 3 cut(s) 936, 977, 1277
Zsp2I ATGCAT 1 cut(s) 1681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.