RLG00000016735

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014871
Physical Location & Seq
Forward (+)
9558977 .. 9559375
399 bp
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UTR
Exon/CDS
Intron
RLM00000016735

Sequence Viewer

Length: 399 bp
ATGGACTGTGGTGGCTTCAAAGTAGACGACATCAAAACGGCGTCGGATACCCGCTACGCCTTGGCCGCAGCCCATCTAACTTATTTCCAGTCGCTCAAGCAAATCGGCGATGCGTTTCGCAAGTCCGTTGACGAGGACCTAGTCATCGGAGGCGTCGACGAGTCGTATTCACTATCGGATTCTCTAGTTTTGACATTGCCCTCCGACGAAGGCAAGCCCGGGAAAGGAAAGCCCCAAGGGAAAAGCTTGTCGTCGTCTTCGATTTCACTCTCGCATAGTTTCGTGGACATTGCAGACTCTCACCTGCATTTATTCGATTCGGATTCTGATTTGGATTCGAGTCATATTCACATTGAGAGTAGCGTCATCGGCGTCACTCTGCCTCAGCGTCGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

133

Amino Acids

14.25

Weight (kDa)

4.99

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 11 - 45 3.6e-10 Protein of unknown function (DUF630)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 312
Acc36I ACCTGC 1 cut(s) 312
AccI GTMKAC 2 cut(s) 24, 156
AciI CCGC 2 cut(s) 52, 66
AcoI YGGCCR 1 cut(s) 63
AcyI GRCGYC 3 cut(s) 41, 153, 372
AfiI CCNNNNNNNGG 1 cut(s) 224
AgsI TTSAA 1 cut(s) 19
AluBI AGCT 1 cut(s) 246
AluI AGCT 1 cut(s) 246
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 1 cut(s) 68
AspS9I GGNCC 1 cut(s) 136
AsuC2I CCSGG 2 cut(s) 219, 220
AsuHPI GGTGA 1 cut(s) 293
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 249
BbvCI CCTCAGC 1 cut(s) 384
BbvI GCAGC 1 cut(s) 80
BccI CCATC 1 cut(s) 81
BceAI ACGGC 1 cut(s) 54
BciVI GTATCC 1 cut(s) 40
BcnI CCSGG 2 cut(s) 219, 220
BfaI CTAG 2 cut(s) 140, 185
BfuAI ACCTGC 1 cut(s) 312
BfuI GTATCC 1 cut(s) 40
BisI GCNGC 2 cut(s) 66, 69
BlsI GCNGC 2 cut(s) 67, 70
Bme1390I CCNGG 2 cut(s) 219, 220
Bme18I GGWCC 1 cut(s) 136
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 136
BmrFI CCNGG 2 cut(s) 219, 220
BmsI GCATC 1 cut(s) 100
BpiI GAAGAC 1 cut(s) 249
Bpu10I CCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 2 cut(s) 219, 220
BsaHI GRCGYC 3 cut(s) 41, 153, 372
BsaJI CCNNGG 3 cut(s) 60, 218, 235
Bsc4I CCNNNNNNNGG 1 cut(s) 224
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 2 cut(s) 194, 288
BseDI CCNNGG 3 cut(s) 60, 218, 235
BseLI CCNNNNNNNGG 1 cut(s) 224
BseMI GCAATG 2 cut(s) 194, 288
BseMII CTCAG 1 cut(s) 398
BseNI ACTGG 1 cut(s) 88
BseXI GCAGC 1 cut(s) 80
BshFI GGCC 1 cut(s) 65
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 224
BsnI GGCC 1 cut(s) 65
BsoBI CYCGRG 1 cut(s) 218
BspACI CCGC 2 cut(s) 52, 66
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 1 cut(s) 397
BspMI ACCTGC 1 cut(s) 312
BsrDI GCAATG 2 cut(s) 194, 288
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 3 cut(s) 60, 218, 235
BssNI GRCGYC 3 cut(s) 41, 153, 372
BssT1I CCWWGG 2 cut(s) 60, 235
Bst4CI ACNGT 1 cut(s) 8
BstACI GRCGYC 3 cut(s) 41, 153, 372
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 1 cut(s) 384
BstMWI GCNNNNNNNGC 2 cut(s) 65, 369
BstSCI CCNGG 2 cut(s) 217, 218
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 249
BsuI GTATCC 1 cut(s) 40
BsuRI GGCC 1 cut(s) 65
BtgZI GCGATG 1 cut(s) 123
BveI ACCTGC 1 cut(s) 312
Cac8I GCNNGC 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 136
Cfr9I CCCGGG 1 cut(s) 218
CseI GACGC 5 cut(s) 30, 142, 352, 361, 377
CviJI RGCY 6 cut(s) 15, 65, 71, 217, 232, 246
CviKI_1 RGCY 6 cut(s) 15, 65, 71, 217, 232, 246
DdeI CTNAG 1 cut(s) 384
EaeI YGGCCR 1 cut(s) 63
Eco130I CCWWGG 2 cut(s) 60, 235
Eco47I GGWCC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 60, 235
ErhI CCWWGG 2 cut(s) 60, 235
FaiI YATR 2 cut(s) 276, 345
FauI CCCGC 1 cut(s) 59
FblI GTMKAC 2 cut(s) 24, 156
Fnu4HI GCNGC 2 cut(s) 66, 69
Fsp4HI GCNGC 2 cut(s) 66, 69
FspBI CTAG 2 cut(s) 140, 185
GluI GCNGC 2 cut(s) 66, 69
HaeIII GGCC 1 cut(s) 65
HapII CCGG 1 cut(s) 219
HgaI GACGC 5 cut(s) 30, 142, 352, 361, 377
Hin1I GRCGYC 3 cut(s) 41, 153, 372
HincII GTYRAC 2 cut(s) 130, 157
HindII GTYRAC 2 cut(s) 130, 157
HindIII AAGCTT 1 cut(s) 244
HinfI GANTC 7 cut(s) 161, 179, 296, 317, 323, 335, 340
HpaII CCGG 1 cut(s) 219
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 4 cut(s) 25, 130, 157, 286
Hpy188I TCNGA 6 cut(s) 46, 149, 178, 205, 322, 328
Hpy8I GTNNAC 4 cut(s) 25, 130, 157, 286
Hpy99I CGWCG 6 cut(s) 46, 158, 161, 209, 256, 393
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 1 cut(s) 8
HpyCH4V TGCA 2 cut(s) 293, 307
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 369
HpyF3I CTNAG 1 cut(s) 384
Hsp92I GRCGYC 3 cut(s) 41, 153, 372
LpnPI CCDG 3 cut(s) 101, 232, 317
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 1 cut(s) 100
MaeI CTAG 2 cut(s) 140, 185
MaeIII GTNAC 1 cut(s) 373
MboII GAAGA 1 cut(s) 249
MlyI GAGTC 3 cut(s) 170, 290, 349
MmeI TCCRAC 2 cut(s) 24, 228
MnlI CCTC 4 cut(s) 127, 143, 211, 393
MspI CCGG 1 cut(s) 219
MspR9I CCNGG 2 cut(s) 219, 220
MwoI GCNNNNNNNGC 2 cut(s) 65, 369
NciI CCSGG 2 cut(s) 219, 220
NmuCI GTSAC 1 cut(s) 373
PaqCI CACCTGC 1 cut(s) 312
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 257
PfeI GAWTC 4 cut(s) 179, 317, 323, 335
PflFI GACNNNGTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 67, 70
PleI GAGTC 3 cut(s) 169, 290, 348
PpsI GAGTC 3 cut(s) 169, 290, 348
PpuMI RGGWCCY 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 136
PspPI GGNCC 1 cut(s) 136
PspPPI RGGWCCY 1 cut(s) 136
PsyI GACNNNGTC 1 cut(s) 140
SalI GTCGAC 1 cut(s) 155
SatI GCNGC 2 cut(s) 66, 69
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 3 cut(s) 170, 290, 349
ScrFI CCNGG 2 cut(s) 219, 220
SetI ASST 3 cut(s) 141, 248, 306
SfaNI GCATC 1 cut(s) 100
SgrDI CGTCGACG 1 cut(s) 155
SinI GGWCC 1 cut(s) 136
SmaI CCCGGG 1 cut(s) 220
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SsiI CCGC 2 cut(s) 52, 66
SspMI CTAG 2 cut(s) 140, 185
StyD4I CCNGG 2 cut(s) 217, 218
StyI CCWWGG 2 cut(s) 60, 235
TaaI ACNGT 1 cut(s) 8
TaqI TCGA 4 cut(s) 156, 260, 315, 338
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 4 cut(s) 179, 317, 323, 335
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 373
TspGWI ACGGA 1 cut(s) 115
TspMI CCCGGG 1 cut(s) 218
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 136
XmaI CCCGGG 1 cut(s) 218
XmiI GTMKAC 2 cut(s) 24, 156
XspI CTAG 2 cut(s) 140, 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.