Rh2BG146200

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014871
Physical Location & Seq
Reverse (-)
12597648 .. 12616795
19148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG146200.1

Sequence Viewer

Length: 591 bp
ATGGACTATGGTGGCTCCAAAGTAGACGACATCAAAACGGCGTCGGATACCCGCTACGCCTTGGACGCAGCCCATCTAACTTATTTCCAGTCGCTCAAGCAAATCGGCGACGCGCTTTGCAAGTTCGTTGACGAGGACCTAGTCATCGGCGGCGTCGGCGAGTCATATTCGCTACTGGATTCTCCAGTTCTGACATTGCCCTCCGTCGAAGGAAGCCCGGGAAAGGAAAGCCCCAAGGGAAAAGCTCCTCGTCGTCTTCGATTTCACTCTCGCATAGTCACCGGGAATGGTGAGTTAGTCCCGAGCCTGAGTTGGAGAAACAGTCAACTAGCTAGTGGATTCAATCAGCAGCTTCATCTGATTTCTTGCGGGACAAATCAAATTCCAATCAAGGCCTTCTCAATCTATGAACATGCTTATTCTTCATGTAAAACAGATTGTCCAATCTGGAAGATTGAGCAAATCATGCTATTTGGTTTTGATGTCTTTGGATTCCTATTTTTATGGAGTTTGGGATTTGTCGCTGGAACAACATCACTGCAAGAAGGTGATTCTTACTTTGATAATTTCGGTTATTGGTTTAGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

196

Amino Acids

21.71

Weight (kDa)

5.3

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 11 - 46 1.2e-09 Protein of unknown function (DUF630)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 24
AccII CGCG 1 cut(s) 113
AciI CCGC 3 cut(s) 52, 150, 369
AcsI RAATTY 1 cut(s) 381
AcyI GRCGYC 2 cut(s) 41, 153
AfiI CCNNNNNNNGG 1 cut(s) 223
AgsI TTSAA 1 cut(s) 343
AluBI AGCT 3 cut(s) 245, 332, 352
AluI AGCT 3 cut(s) 245, 332, 352
Ama87I CYCGRG 2 cut(s) 217, 301
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 2 cut(s) 68, 349
ApoI RAATTY 1 cut(s) 381
AspLEI GCGC 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 136
AsuC2I CCSGG 3 cut(s) 218, 219, 283
AsuHPI GGTGA 3 cut(s) 271, 302, 560
AvaI CYCGRG 2 cut(s) 217, 301
AvaII GGWCC 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 248
BbvI GCAGC 2 cut(s) 80, 361
BccI CCATC 1 cut(s) 81
BceAI ACGGC 1 cut(s) 54
BciVI GTATCC 1 cut(s) 40
BcnI CCSGG 3 cut(s) 218, 219, 283
BfaI CTAG 3 cut(s) 140, 329, 333
BfuI GTATCC 1 cut(s) 40
BisI GCNGC 3 cut(s) 69, 151, 350
BlsI GCNGC 3 cut(s) 70, 152, 351
Bme1390I CCNGG 3 cut(s) 218, 219, 283
Bme18I GGWCC 1 cut(s) 136
BmeT110I CYCGRG 2 cut(s) 217, 301
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 16
BmrFI CCNGG 3 cut(s) 218, 219, 283
BpiI GAAGAC 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 3 cut(s) 218, 219, 283
BsaHI GRCGYC 2 cut(s) 41, 153
BsaJI CCNNGG 3 cut(s) 60, 217, 234
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse1I ACTGG 3 cut(s) 88, 180, 185
Bse3DI GCAATG 1 cut(s) 194
BseDI CCNNGG 3 cut(s) 60, 217, 234
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMI GCAATG 1 cut(s) 194
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 3 cut(s) 88, 180, 185
BseRI GAGGAG 1 cut(s) 237
BseXI GCAGC 2 cut(s) 80, 361
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 1 cut(s) 395
BsiHKCI CYCGRG 2 cut(s) 217, 301
BsiSI CCGG 2 cut(s) 218, 282
BslFI GGGAC 2 cut(s) 284, 385
BslI CCNNNNNNNGG 1 cut(s) 223
BsmFI GGGAC 2 cut(s) 284, 385
BsnI GGCC 1 cut(s) 395
BsoBI CYCGRG 2 cut(s) 217, 301
BspACI CCGC 3 cut(s) 52, 150, 369
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 1 cut(s) 300
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 194
BsrI ACTGG 3 cut(s) 88, 180, 185
BssECI CCNNGG 3 cut(s) 60, 217, 234
BssNI GRCGYC 2 cut(s) 41, 153
BssT1I CCWWGG 2 cut(s) 60, 234
Bst4CI ACNGT 1 cut(s) 323
BstACI GRCGYC 2 cut(s) 41, 153
BstAPI GCANNNNNTGC 1 cut(s) 466
BstDEI CTNAG 1 cut(s) 308
BstFNI CGCG 1 cut(s) 113
BstHHI GCGC 1 cut(s) 115
BstMWI GCNNNNNNNGC 3 cut(s) 65, 156, 466
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 3 cut(s) 216, 217, 281
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 2 cut(s) 80, 361
BstV2I GAAGAC 1 cut(s) 248
BsuI GTATCC 1 cut(s) 40
BsuRI GGCC 1 cut(s) 395
BtsI GCAGTG 1 cut(s) 536
BtsIMutI CAGTG 1 cut(s) 536
CfoI GCGC 1 cut(s) 115
Cfr13I GGNCC 1 cut(s) 136
Cfr9I CCCGGG 1 cut(s) 217
CseI GACGC 4 cut(s) 30, 74, 119, 142
CviAII CATG 3 cut(s) 413, 426, 466
CviJI RGCY 9 cut(s) 15, 71, 216, 231, 245, 306, 332, 352, 395
CviKI_1 RGCY 9 cut(s) 15, 71, 216, 231, 245, 306, 332, 352, 395
DdeI CTNAG 1 cut(s) 308
Eco130I CCWWGG 2 cut(s) 60, 234
Eco147I AGGCCT 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 136
Eco88I CYCGRG 2 cut(s) 217, 301
EcoO109I RGGNCCY 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 60, 234
ErhI CCWWGG 2 cut(s) 60, 234
FaeI CATG 3 cut(s) 416, 429, 469
FaiI YATR 8 cut(s) 9, 166, 275, 408, 414, 427, 467, 505
FaqI GGGAC 2 cut(s) 284, 385
FatI CATG 3 cut(s) 412, 425, 465
FauI CCCGC 2 cut(s) 59, 362
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 3 cut(s) 69, 151, 350
Fsp4HI GCNGC 3 cut(s) 69, 151, 350
FspBI CTAG 3 cut(s) 140, 329, 333
GlaI GCGC 1 cut(s) 114
GluI GCNGC 3 cut(s) 69, 151, 350
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 1 cut(s) 395
HapII CCGG 2 cut(s) 218, 282
HgaI GACGC 4 cut(s) 30, 74, 119, 142
HhaI GCGC 1 cut(s) 115
Hin1I GRCGYC 2 cut(s) 41, 153
Hin1II CATG 3 cut(s) 416, 429, 469
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HincII GTYRAC 2 cut(s) 130, 326
HindII GTYRAC 2 cut(s) 130, 326
HinfI GANTC 5 cut(s) 161, 179, 339, 492, 551
HpaII CCGG 2 cut(s) 218, 282
HphI GGTGA 3 cut(s) 271, 302, 560
Hpy166II GTNNAC 3 cut(s) 25, 130, 326
Hpy188I TCNGA 3 cut(s) 46, 192, 360
Hpy188III TCNNGA 2 cut(s) 301, 448
Hpy8I GTNNAC 3 cut(s) 25, 130, 326
Hpy99I CGWCG 5 cut(s) 46, 113, 158, 209, 255
HpyAV CCTTC 3 cut(s) 203, 406, 539
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4V TGCA 2 cut(s) 120, 541
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 156, 466
HpyF3I CTNAG 1 cut(s) 308
Hsp92I GRCGYC 2 cut(s) 41, 153
Hsp92II CATG 3 cut(s) 416, 429, 469
HspAI GCGC 1 cut(s) 113
LmnI GCTCC 2 cut(s) 20, 250
LpnPI CCDG 8 cut(s) 101, 161, 198, 231, 295, 320, 433, 510
Lsp1109I GCAGC 2 cut(s) 80, 361
MaeI CTAG 3 cut(s) 140, 329, 333
MaeIII GTNAC 1 cut(s) 277
MboII GAAGA 3 cut(s) 248, 414, 463
MfeI CAATTG 1 cut(s) 586
MluCI AATT 3 cut(s) 381, 565, 586
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 2 cut(s) 24, 293
MnlI CCTC 3 cut(s) 127, 211, 258
MspI CCGG 2 cut(s) 218, 282
MspR9I CCNGG 3 cut(s) 218, 219, 283
MunI CAATTG 1 cut(s) 586
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 3 cut(s) 65, 156, 466
NciI CCSGG 3 cut(s) 218, 219, 283
NlaIII CATG 3 cut(s) 416, 429, 469
NlaIV GGNNCC 1 cut(s) 16
NmuCI GTSAC 1 cut(s) 277
NspI RCATGY 1 cut(s) 416
PceI AGGCCT 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 256
PfeI GAWTC 4 cut(s) 179, 339, 492, 551
PflFI GACNNNGTC 1 cut(s) 140
PkrI GCNGC 3 cut(s) 70, 152, 351
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
PpuMI RGGWCCY 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 16
PspPI GGNCC 1 cut(s) 136
PspPPI RGGWCCY 1 cut(s) 136
PsyI GACNNNGTC 1 cut(s) 140
SatI GCNGC 3 cut(s) 69, 151, 350
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 3 cut(s) 218, 219, 283
SetI ASST 5 cut(s) 141, 247, 334, 354, 550
SinI GGWCC 1 cut(s) 136
SmaI CCCGGG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 3 cut(s) 381, 565, 586
SseBI AGGCCT 1 cut(s) 395
SsiI CCGC 3 cut(s) 52, 150, 369
SspMI CTAG 3 cut(s) 140, 329, 333
StuI AGGCCT 1 cut(s) 395
StyD4I CCNGG 3 cut(s) 216, 217, 281
StyI CCWWGG 2 cut(s) 60, 234
TaaI ACNGT 1 cut(s) 323
TaqI TCGA 2 cut(s) 207, 259
TasI AATT 3 cut(s) 381, 565, 586
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 4 cut(s) 179, 339, 492, 551
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 2 cut(s) 68, 349
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 3 cut(s) 344, 414, 423
TspGWI ACGGA 1 cut(s) 193
TspMI CCCGGG 1 cut(s) 217
TspRI CASTG 1 cut(s) 543
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 136
XapI RAATTY 1 cut(s) 381
XceI RCATGY 1 cut(s) 416
XmaI CCCGGG 1 cut(s) 217
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 3 cut(s) 140, 329, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.