Rh4CG060400

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014871
Physical Location & Seq
Reverse (-)
11585771 .. 11590460
4690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG060400.1

Sequence Viewer

Length: 1413 bp
ATGAGCTTCTCAGCTTTCTCTGTGCTTTCAGTTTGTCGCAATCATCATCTGCTTCAGCTATTTGGAGGCCCACAGTGGCTTACCGTCAAATGGGGTTCACATTGTTATGTCAAGAAGGTGAAAGAAGTGTTGGAGAGTAAACGGTGTCAGATAAGAACCGGCTCTGAGGTGCATAAAGTTTCCACAACTGATGAGGGTTGCACTGTTTTTACTGGAGATGGTATTGGGGAAATTTACAATAAATGCATAATAGCTATCCATTCCCCTGATGCCGTGAGAATATTAGGAGAACAGGCAACATCTGATGAACTGAGAATACTTGGCGCCTTCCAAAATGTGTACTGTGATATTTTCCTTCATCGTGACAAAACTTTAATGCCCCAAAATCTAGCAGCATGGAGTGCATTGAACTATCTTGGAAGTACTGACAACAAAGTATGTTTGACATACTGGCTCAATCAGCATCAGAGCCTCAGTGAAACAAGTCAACCATATCTTGTGACACTCAATCCAGATCATACACCAAACCACACCTTGCTTAAGTGGTCAACAAGCCATCCAGTCCTATCTGTTGCGGCATCAAAAGCTTCGCTGGAGTTTCCTCATATTCAAGGAAAGAGAGGAATTTGGTTTTGTGGAGCATACCAGGGTTACGGCTTCCATGAGGATGGGCTAAAGGCTGGAATGGCTGCAGCACATGGTATGCTTGGAAAAGGTTGTGCCGTGTTGAGAAACCCAAAACATATGGCTCTTTCAATGACGGAAACAGCGGCACGCCTGTTTGTTATTAGATTTCTTAGACATTATATATCTACAGGATGTTTAATTTTATTAGAAGAAGGAGGCTCGATGTTCACCTTTGAAGGAAGCAGAAAGGACTGTTCTCTAAAATCCGTTCTTAAAGTTCACAGTCCTCAGTTTTACTGGAAAGCTAGAATTGATGAGAACCATGAGGTTCTAGATATTGGTTTTGGTTGGGGAACCTTTGCTATTGAAGCAGTCAAACAAACGGGCTGCAGATACACTGGCATCACTCTATCTGAGGAACAACTTAAATTTGCTGAAAAGAAAGTGAAAGATGCTGGACTTCAGCACCATATCAAGTTCCTTCTGTGTGACTACCGCCAGTTGCCTTCTGACCACAAATATGACAGAATCATTTGTTGCGAGATGATAGAACATGTCGGCCATGAATTTGTGGATGAGTTCTTTTCTTGTTGTGAGTCTGTACTGGCAAAAAATGGGCTTTTTATTCTCCAGTTCATATCCACACCGGATGAACGCTACGATAACTACAGGCGAAGTTCAGACTTCATAAAGGAGTATATGTTTCCTGGTGCATGTGTACTTTCATTAAGTAGGGTAATATCAGCTATGGCCGCTTCTTCCAGACTCTGGTATGACCTGTCATGA

Protein Analysis

470

Amino Acids

53.06

Weight (kDa)

7.56

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 307 - 460 2.9e-35 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 312 - 426 4.2e-09 Methyltransferase domain
Methyltransf_31 PF13847 314 - 429 7.7e-12 Methyltransferase domain
Methyltransf_25 PF13649 319 - 415 8.3e-17 Methyltransferase domain
Methyltransf_11 PF08241 320 - 419 2.2e-14 Methyltransferase domain
Methyltransf_12 PF08242 320 - 417 1.6e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 1395
AciI CCGC 4 cut(s) 575, 770, 1123, 1380
AcoI YGGCCR 2 cut(s) 1186, 1377
AcsI RAATTY 4 cut(s) 231, 624, 1055, 1193
AcuI CTGAAG 2 cut(s) 38, 1073
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 4 cut(s) 341, 424, 1230, 1347
AfiI CCNNNNNNNGG 2 cut(s) 90, 1395
AflII CTTAAG 1 cut(s) 539
AflIII ACRYGT 1 cut(s) 1180
AgsI TTSAA 5 cut(s) 409, 611, 756, 863, 995
AjnI CCWGG 2 cut(s) 645, 1333
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AluBI AGCT 7 cut(s) 6, 14, 58, 254, 587, 932, 1373
AluI AGCT 7 cut(s) 6, 14, 58, 254, 587, 932, 1373
AlwNI CAGNNNCTG 1 cut(s) 1395
AoxI GGCC 3 cut(s) 67, 1186, 1377
ApeKI GCWGC 4 cut(s) 392, 689, 692, 1014
ApoI RAATTY 4 cut(s) 231, 624, 1055, 1193
AspLEI GCGC 1 cut(s) 326
AspS9I GGNCC 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 130, 847
BanI GGYRCC 1 cut(s) 323
BbvI GCAGC 4 cut(s) 404, 676, 704, 1001
BccI CCATC 3 cut(s) 212, 564, 662
BceAI ACGGC 3 cut(s) 257, 670, 707
BciT130I CCWGG 2 cut(s) 647, 1335
BfaI CTAG 3 cut(s) 389, 933, 959
BfmI CTRYAG 4 cut(s) 690, 813, 1015, 1294
BfoI RGCGCY 1 cut(s) 327
BfrI CTTAAG 1 cut(s) 539
BisI GCNGC 7 cut(s) 393, 576, 690, 693, 771, 1015, 1380
BlsI GCNGC 7 cut(s) 394, 577, 691, 694, 772, 1016, 1381
BmcAI AGTACT 1 cut(s) 424
Bme1390I CCNGG 2 cut(s) 647, 1335
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 2 cut(s) 325, 982
BmrFI CCNGG 2 cut(s) 647, 1335
BmsI GCATC 5 cut(s) 259, 472, 587, 1038, 1069
BpmI CTGGAG 3 cut(s) 234, 614, 1241
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 646
BsaWI WCCGGW 1 cut(s) 1273
BsaXI ACNNNNNCTCC 2 cut(s) 1313, 1343
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 1395
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 8 cut(s) 217, 455, 560, 929, 1030, 1126, 1236, 1258
BseBI CCWGG 2 cut(s) 647, 1335
BseDI CCNNGG 1 cut(s) 646
BseGI GGATG 5 cut(s) 556, 673, 824, 1207, 1282
BseLI CCNNNNNNNGG 2 cut(s) 90, 1395
BseMII CTCAG 6 cut(s) 24, 156, 302, 487, 929, 1032
BseNI ACTGG 8 cut(s) 217, 455, 560, 929, 1030, 1126, 1236, 1258
BseXI GCAGC 4 cut(s) 404, 676, 704, 1001
BshFI GGCC 3 cut(s) 69, 1188, 1379
BshNI GGYRCC 1 cut(s) 323
BsiSI CCGG 2 cut(s) 159, 1274
BslI CCNNNNNNNGG 2 cut(s) 90, 1395
BsnI GGCC 3 cut(s) 69, 1188, 1379
Bsp143I GATC 1 cut(s) 514
BspACI CCGC 4 cut(s) 575, 770, 1123, 1380
BspANI GGCC 3 cut(s) 69, 1188, 1379
BspCNI CTCAG 6 cut(s) 23, 157, 303, 486, 928, 1033
BspHI TCATGA 1 cut(s) 1409
BspLI GGNNCC 2 cut(s) 325, 982
BspMAI CTGCAG 2 cut(s) 694, 1019
BspT107I GGYRCC 1 cut(s) 323
BspTI CTTAAG 1 cut(s) 539
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 8 cut(s) 217, 455, 560, 929, 1030, 1126, 1236, 1258
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 646
BssMI GATC 1 cut(s) 514
BssNI GRCGYC 1 cut(s) 324
Bst2UI CCWGG 2 cut(s) 647, 1335
Bst4CI ACNGT 7 cut(s) 75, 85, 144, 205, 344, 881, 911
BstACI GRCGYC 1 cut(s) 324
BstAFI CTTAAG 1 cut(s) 539
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 775
BstDEI CTNAG 7 cut(s) 10, 165, 311, 473, 797, 915, 1041
BstF5I GGATG 5 cut(s) 556, 673, 824, 1207, 1282
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 1 cut(s) 326
BstKTI GATC 1 cut(s) 517
BstMBI GATC 1 cut(s) 514
BstMWI GCNNNNNNNGC 6 cut(s) 401, 460, 584, 686, 995, 1379
BstNI CCWGG 2 cut(s) 647, 1335
BstNSI RCATGY 2 cut(s) 1184, 1344
BstSCI CCNGG 2 cut(s) 645, 1333
BstSFI CTRYAG 4 cut(s) 690, 813, 1015, 1294
BstV1I GCAGC 4 cut(s) 404, 676, 704, 1001
BstXI CCANNNNNNTGG 1 cut(s) 668
BsuRI GGCC 3 cut(s) 69, 1188, 1379
BtsCI GGATG 5 cut(s) 556, 673, 824, 1207, 1282
BtsIMutI CAGTG 4 cut(s) 80, 201, 481, 1023
Cac8I GCNNGC 1 cut(s) 775
CaiI CAGNNNCTG 1 cut(s) 1395
CciI TCATGA 1 cut(s) 1409
CfoI GCGC 1 cut(s) 326
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 68
Csp6I GTAC 4 cut(s) 340, 423, 1229, 1346
CviAII CATG 8 cut(s) 396, 662, 698, 950, 1181, 1190, 1341, 1410
CviQI GTAC 4 cut(s) 340, 423, 1229, 1346
DdeI CTNAG 7 cut(s) 10, 165, 311, 473, 797, 915, 1041
DinI GGCGCC 1 cut(s) 325
DpnI GATC 1 cut(s) 516
DpnII GATC 1 cut(s) 514
EaeI YGGCCR 2 cut(s) 1186, 1377
Eco57I CTGAAG 2 cut(s) 38, 1073
EcoRII CCWGG 2 cut(s) 645, 1333
EcoT22I ATGCAT 1 cut(s) 248
EgeI GGCGCC 1 cut(s) 325
EheI GGCGCC 1 cut(s) 325
FaeI CATG 8 cut(s) 399, 665, 701, 953, 1184, 1193, 1344, 1413
FatI CATG 8 cut(s) 395, 661, 697, 949, 1180, 1189, 1340, 1409
FauNDI CATATG 1 cut(s) 744
Fnu4HI GCNGC 7 cut(s) 393, 576, 690, 693, 771, 1015, 1380
FokI GGATG 5 cut(s) 543, 680, 831, 1214, 1289
Fsp4HI GCNGC 7 cut(s) 393, 576, 690, 693, 771, 1015, 1380
FspBI CTAG 3 cut(s) 389, 933, 959
GlaI GCGC 1 cut(s) 325
GluI GCNGC 7 cut(s) 393, 576, 690, 693, 771, 1015, 1380
GsuI CTGGAG 3 cut(s) 234, 614, 1241
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 3 cut(s) 69, 1188, 1379
HapII CCGG 2 cut(s) 159, 1274
HhaI GCGC 1 cut(s) 326
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 8 cut(s) 399, 665, 701, 953, 1184, 1193, 1344, 1413
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HincII GTYRAC 2 cut(s) 488, 549
HindII GTYRAC 2 cut(s) 488, 549
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 3 cut(s) 1155, 1223, 1392
HpaII CCGG 2 cut(s) 159, 1274
HphI GGTGA 2 cut(s) 130, 847
Hpy166II GTNNAC 8 cut(s) 98, 140, 340, 488, 549, 855, 907, 1346
Hpy188I TCNGA 7 cut(s) 150, 166, 304, 468, 1042, 1138, 1309
Hpy188III TCNNGA 6 cut(s) 112, 362, 512, 959, 1389, 1410
Hpy8I GTNNAC 8 cut(s) 98, 140, 340, 488, 549, 855, 907, 1346
HpyAV CCTTC 7 cut(s) 109, 337, 365, 833, 857, 1118, 1143
HpyCH4III ACNGT 7 cut(s) 75, 85, 144, 205, 344, 881, 911
HpyCH4V TGCA 7 cut(s) 172, 201, 246, 404, 692, 1017, 1340
HpyF10VI GCNNNNNNNGC 6 cut(s) 401, 460, 584, 686, 995, 1379
HpyF3I CTNAG 7 cut(s) 10, 165, 311, 473, 797, 915, 1041
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 8 cut(s) 399, 665, 701, 953, 1184, 1193, 1344, 1413
HspAI GCGC 1 cut(s) 324
KasI GGCGCC 1 cut(s) 323
Kzo9I GATC 1 cut(s) 514
LmnI GCTCC 1 cut(s) 638
Lsp1109I GCAGC 4 cut(s) 404, 676, 704, 1001
LweI GCATC 5 cut(s) 259, 472, 587, 1038, 1069
MaeI CTAG 3 cut(s) 389, 933, 959
MaeIII GTNAC 4 cut(s) 362, 499, 650, 1115
MalI GATC 1 cut(s) 516
MboI GATC 1 cut(s) 514
MboII GAAGA 2 cut(s) 848, 1377
MluCI AATT 6 cut(s) 231, 624, 825, 936, 1055, 1193
Mly113I GGCGCC 1 cut(s) 324
MlyI GAGTC 2 cut(s) 1232, 1386
MmeI TCCRAC 1 cut(s) 111
Mph1103I ATGCAT 1 cut(s) 248
MseI TTAA 6 cut(s) 374, 540, 824, 900, 1053, 1355
MslI CAYNNNNRTG 3 cut(s) 105, 666, 1146
MspA1I CMGCKG 1 cut(s) 770
MspCI CTTAAG 1 cut(s) 539
MspI CCGG 2 cut(s) 159, 1274
MspR9I CCNGG 2 cut(s) 647, 1335
MvaI CCWGG 2 cut(s) 647, 1335
MwoI GCNNNNNNNGC 6 cut(s) 401, 460, 584, 686, 995, 1379
NarI GGCGCC 1 cut(s) 324
NdeI CATATG 1 cut(s) 744
NdeII GATC 1 cut(s) 514
NlaIII CATG 8 cut(s) 399, 665, 701, 953, 1184, 1193, 1344, 1413
NlaIV GGNNCC 2 cut(s) 325, 982
NmuCI GTSAC 3 cut(s) 362, 499, 1115
NsiI ATGCAT 1 cut(s) 248
NspI RCATGY 2 cut(s) 1184, 1344
PagI TCATGA 1 cut(s) 1409
PciI ACATGT 1 cut(s) 1180
PfeI GAWTC 1 cut(s) 1155
PflMI CCANNNNNTGG 1 cut(s) 1395
PkrI GCNGC 7 cut(s) 394, 577, 691, 694, 772, 1016, 1381
PleI GAGTC 2 cut(s) 1231, 1386
PluTI GGCGCC 1 cut(s) 327
PpsI GAGTC 2 cut(s) 1231, 1386
PscI ACATGT 1 cut(s) 1180
Psp6I CCWGG 2 cut(s) 645, 1333
PspGI CCWGG 2 cut(s) 645, 1333
PspN4I GGNNCC 2 cut(s) 325, 982
PspPI GGNCC 1 cut(s) 68
PsrI GAACNNNNNNTAC 2 cut(s) 300, 332
PstI CTGCAG 2 cut(s) 694, 1019
PstNI CAGNNNCTG 1 cut(s) 1395
RsaI GTAC 4 cut(s) 341, 424, 1230, 1347
RsaNI GTAC 4 cut(s) 340, 423, 1229, 1346
RseI CAYNNNNRTG 3 cut(s) 105, 666, 1146
SaqAI TTAA 6 cut(s) 374, 540, 824, 900, 1053, 1355
SatI GCNGC 7 cut(s) 393, 576, 690, 693, 771, 1015, 1380
Sau3AI GATC 1 cut(s) 514
Sau96I GGNCC 1 cut(s) 68
ScaI AGTACT 1 cut(s) 424
SchI GAGTC 2 cut(s) 1232, 1386
ScrFI CCNGG 2 cut(s) 647, 1335
SfaNI GCATC 5 cut(s) 259, 472, 587, 1038, 1069
SfcI CTRYAG 4 cut(s) 690, 813, 1015, 1294
SfoI GGCGCC 1 cut(s) 325
SmiMI CAYNNNNRTG 3 cut(s) 105, 666, 1146
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 6 cut(s) 231, 624, 825, 936, 1055, 1193
SsiI CCGC 4 cut(s) 575, 770, 1123, 1380
SspDI GGCGCC 1 cut(s) 323
SspI AATATT 1 cut(s) 282
SspMI CTAG 3 cut(s) 389, 933, 959
StyD4I CCNGG 2 cut(s) 645, 1333
TaaI ACNGT 7 cut(s) 75, 85, 144, 205, 344, 881, 911
TaqI TCGA 1 cut(s) 848
TasI AATT 6 cut(s) 231, 624, 825, 936, 1055, 1193
TatI WGTACW 4 cut(s) 339, 422, 1228, 1345
TauI GCSGC 3 cut(s) 578, 773, 1382
TfiI GAWTC 1 cut(s) 1155
Tru1I TTAA 6 cut(s) 374, 540, 824, 900, 1053, 1355
Tru9I TTAA 6 cut(s) 374, 540, 824, 900, 1053, 1355
TscAI CASTG 4 cut(s) 80, 208, 481, 1030
TseFI GTSAC 3 cut(s) 362, 499, 1115
TseI GCWGC 4 cut(s) 392, 689, 692, 1014
Tsp45I GTSAC 3 cut(s) 362, 499, 1115
TspDTI ATGAA 7 cut(s) 321, 347, 1206, 1252, 1293, 1303, 1341
TspGWI ACGGA 2 cut(s) 776, 883
TspRI CASTG 4 cut(s) 80, 208, 481, 1030
Van91I CCANNNNNTGG 1 cut(s) 1395
Vha464I CTTAAG 1 cut(s) 539
XapI RAATTY 4 cut(s) 231, 624, 1055, 1193
XbaI TCTAGA 1 cut(s) 958
XceI RCATGY 2 cut(s) 1184, 1344
XspI CTAG 3 cut(s) 389, 933, 959
ZrmI AGTACT 1 cut(s) 424
Zsp2I ATGCAT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.