Rh4BG053200

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014871
Physical Location & Seq
Reverse (-)
9506982 .. 9514096
7115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG053200.1

Sequence Viewer

Length: 978 bp
ATGGACTGTGGTGGCTCCAAAGTAGACGACATCAAAACGGCGTCAGATACCCGCTACGCCTTGGACGCAACCCATCTAACTTATTTCCAGTCGCTCAAGCAAATCGGCGACGCGCTTTGCAAGTTCGTTGACGAGGACCTAGTCATCGACGGCGTCGGCGAGTCATATTCGCTACCGGATTCTCCAGTTCTGACATTGCCCTCCGTCGAAGGAAGCCCAGGAAAGGAAAGCCCCAAGGGAAAAGCTCCTCGTCGTCTTCGATTTCACTCTCGCATAGTCACCAGAATGGTGAAAGAAGTGTTGGAGAGTAAGGGTTGTCAGATAAGAACCGGCTGTGAGGTACATAAAGTTTCCACAACTGATGAGGGTTGCACTGTTTTTACTGGGGATGGTTTTGAGGAAATTTACAATGGATGCATTATTGCTATTCAAGCCCCCAAAGCCGTGAGAATATTAGCAGATCAGGCAACACCTGATGAATTGAGAATACTTGGCGCCTTCCAATATGTGTACCGTGATATTTTCCTTCATCGTGACAAAACTTTAATGCCCCAAAACCTAGCAGCATGGAGTGCATTGAACTATCTTGGAAGTACTGACAACAAAGTATGTTTGACATACTGGCTCAATCAGCTTCAGGACCTCGGTGAAACAAGTCAACCATATCTTGTGACACTCAATCCAGATCATACACCAAACCACACCTTGCTTAAGTGGTCAACAAGCCATCCAGTCCTATCTGTTGCAGCATCAAAAGCTTCGCTGGATCTTCCTCATATTCAAGGAAAGAGAGGAATTTGGTTTTGTGGAGCATACCAGGGTTATGGCTTCCATGAGGATGGGCTAAAGTTTTATCCCCATTGTAATAAAGGGGGAAAAAAAAAACACTTCACCTATCACCAGTTCAACATCAACGCAAAGTCGCAAACCCTCACCACCACCATAATTGAAACTGTATGTATCAGAGAAAAACATTGA

Protein Analysis

325

Amino Acids

36.24

Weight (kDa)

7.18

Isoelectric Point (pI)

34.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 11 - 46 1.5e-08 Protein of unknown function (DUF630)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 494
AccI GTMKAC 1 cut(s) 24
AccII CGCG 1 cut(s) 113
AciI CCGC 1 cut(s) 52
AclWI GGATC 1 cut(s) 774
AcsI RAATTY 2 cut(s) 402, 795
AcuI CTGAAG 1 cut(s) 620
AcyI GRCGYC 3 cut(s) 41, 153, 495
AfaI GTAC 3 cut(s) 342, 512, 595
AfiI CCNNNNNNNGG 1 cut(s) 223
AflII CTTAAG 1 cut(s) 710
AgsI TTSAA 5 cut(s) 431, 580, 782, 907, 950
AjnI CCWGG 2 cut(s) 217, 816
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 3 cut(s) 245, 634, 758
AluI AGCT 3 cut(s) 245, 634, 758
AlwI GGATC 1 cut(s) 774
ApeKI GCWGC 2 cut(s) 563, 746
ApoI RAATTY 2 cut(s) 402, 795
AspLEI GCGC 2 cut(s) 115, 497
AspS9I GGNCC 2 cut(s) 136, 640
AsuHPI GGTGA 6 cut(s) 271, 301, 659, 883, 890, 925
AvaII GGWCC 2 cut(s) 136, 640
BanI GGYRCC 1 cut(s) 494
BbsI GAAGAC 1 cut(s) 248
BbvI GCAGC 2 cut(s) 575, 758
BccI CCATC 4 cut(s) 81, 383, 735, 833
BceAI ACGGC 3 cut(s) 54, 166, 428
BciT130I CCWGG 2 cut(s) 219, 818
BfaI CTAG 2 cut(s) 140, 560
BfoI RGCGCY 1 cut(s) 498
BfrI CTTAAG 1 cut(s) 710
BisI GCNGC 2 cut(s) 564, 747
BlsI GCNGC 2 cut(s) 565, 748
BmcAI AGTACT 1 cut(s) 595
Bme1390I CCNGG 2 cut(s) 219, 818
Bme18I GGWCC 2 cut(s) 136, 640
BmgT120I GGNCC 2 cut(s) 136, 640
BmiI GGNNCC 2 cut(s) 16, 496
BmrFI CCNGG 2 cut(s) 219, 818
BmrI ACTGGG 1 cut(s) 393
BmsI GCATC 2 cut(s) 404, 758
BmuI ACTGGG 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 80
BsaHI GRCGYC 3 cut(s) 41, 153, 495
BsaJI CCNNGG 5 cut(s) 60, 217, 234, 643, 817
BsaWI WCCGGW 1 cut(s) 175
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 6 cut(s) 88, 185, 388, 626, 731, 901
Bse3DI GCAATG 1 cut(s) 194
BseBI CCWGG 2 cut(s) 219, 818
BseDI CCNNGG 5 cut(s) 60, 217, 234, 643, 817
BseGI GGATG 4 cut(s) 394, 419, 727, 844
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMI GCAATG 1 cut(s) 194
BseNI ACTGG 6 cut(s) 88, 185, 388, 626, 731, 901
BseRI GAGGAG 1 cut(s) 237
BseXI GCAGC 2 cut(s) 575, 758
Bsh1236I CGCG 1 cut(s) 113
BshNI GGYRCC 1 cut(s) 494
BsiSI CCGG 2 cut(s) 176, 330
BslI CCNNNNNNNGG 1 cut(s) 223
Bsp143I GATC 3 cut(s) 460, 685, 766
BspACI CCGC 1 cut(s) 52
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 2 cut(s) 16, 496
BspPI GGATC 1 cut(s) 774
BspT107I GGYRCC 1 cut(s) 494
BspTI CTTAAG 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 329
BsrI ACTGG 6 cut(s) 88, 185, 388, 626, 731, 901
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 5 cut(s) 60, 217, 234, 643, 817
BssMI GATC 3 cut(s) 460, 685, 766
BssNI GRCGYC 3 cut(s) 41, 153, 495
BssT1I CCWWGG 2 cut(s) 60, 234
Bst2UI CCWGG 2 cut(s) 219, 818
Bst4CI ACNGT 4 cut(s) 8, 376, 515, 955
BstACI GRCGYC 3 cut(s) 41, 153, 495
BstAFI CTTAAG 1 cut(s) 710
BstAPI GCANNNNNTGC 1 cut(s) 572
BstF5I GGATG 4 cut(s) 394, 419, 727, 844
BstFNI CGCG 1 cut(s) 113
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 2 cut(s) 115, 497
BstKTI GATC 3 cut(s) 463, 688, 769
BstMBI GATC 3 cut(s) 460, 685, 766
BstMWI GCNNNNNNNGC 7 cut(s) 65, 431, 440, 464, 572, 631, 755
BstNI CCWGG 2 cut(s) 219, 818
BstSCI CCNGG 2 cut(s) 217, 816
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 2 cut(s) 575, 758
BstV2I GAAGAC 1 cut(s) 248
BstX2I RGATCY 1 cut(s) 766
BstXI CCANNNNNNTGG 2 cut(s) 824, 839
BstYI RGATCY 1 cut(s) 766
BtsCI GGATG 4 cut(s) 394, 419, 727, 844
BtsIMutI CAGTG 1 cut(s) 372
CfoI GCGC 2 cut(s) 115, 497
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 2 cut(s) 136, 640
CseI GACGC 4 cut(s) 30, 74, 119, 142
Csp6I GTAC 3 cut(s) 341, 511, 594
CspCI CAANNNNNGTGG 2 cut(s) 928, 963
CviAII CATG 2 cut(s) 567, 833
CviQI GTAC 3 cut(s) 341, 511, 594
DinI GGCGCC 1 cut(s) 496
DpnI GATC 3 cut(s) 462, 687, 768
DpnII GATC 3 cut(s) 460, 685, 766
Eco130I CCWWGG 2 cut(s) 60, 234
Eco47I GGWCC 2 cut(s) 136, 640
Eco57I CTGAAG 1 cut(s) 620
EcoO109I RGGNCCY 2 cut(s) 136, 640
EcoRII CCWGG 2 cut(s) 217, 816
EcoT14I CCWWGG 2 cut(s) 60, 234
EcoT22I ATGCAT 1 cut(s) 419
EgeI GGCGCC 1 cut(s) 496
EheI GGCGCC 1 cut(s) 496
ErhI CCWWGG 2 cut(s) 60, 234
FaeI CATG 2 cut(s) 570, 836
FatI CATG 2 cut(s) 566, 832
FauI CCCGC 1 cut(s) 59
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 564, 747
FokI GGATG 4 cut(s) 401, 426, 714, 851
Fsp4HI GCNGC 2 cut(s) 564, 747
FspBI CTAG 2 cut(s) 140, 560
GlaI GCGC 2 cut(s) 114, 496
GluI GCNGC 2 cut(s) 564, 747
GsuI CTGGAG 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 498
HapII CCGG 2 cut(s) 176, 330
HgaI GACGC 4 cut(s) 30, 74, 119, 142
HhaI GCGC 2 cut(s) 115, 497
Hin1I GRCGYC 3 cut(s) 41, 153, 495
Hin1II CATG 2 cut(s) 570, 836
Hin6I GCGC 2 cut(s) 113, 495
HinP1I GCGC 2 cut(s) 113, 495
HincII GTYRAC 3 cut(s) 130, 659, 720
HindII GTYRAC 3 cut(s) 130, 659, 720
HindIII AAGCTT 1 cut(s) 756
HinfI GANTC 2 cut(s) 161, 179
HpaII CCGG 2 cut(s) 176, 330
HphI GGTGA 6 cut(s) 271, 301, 659, 883, 890, 925
Hpy166II GTNNAC 5 cut(s) 25, 130, 511, 659, 720
Hpy188I TCNGA 4 cut(s) 46, 192, 321, 965
Hpy188III TCNNGA 3 cut(s) 533, 638, 683
Hpy8I GTNNAC 5 cut(s) 25, 130, 511, 659, 720
Hpy99I CGWCG 5 cut(s) 113, 152, 158, 209, 255
HpyAV CCTTC 3 cut(s) 203, 508, 536
HpyCH4III ACNGT 4 cut(s) 8, 376, 515, 955
HpyCH4V TGCA 5 cut(s) 120, 372, 417, 575, 746
HpyF10VI GCNNNNNNNGC 7 cut(s) 65, 431, 440, 464, 572, 631, 755
Hsp92I GRCGYC 3 cut(s) 41, 153, 495
Hsp92II CATG 2 cut(s) 570, 836
HspAI GCGC 2 cut(s) 113, 495
KasI GGCGCC 1 cut(s) 494
Kzo9I GATC 3 cut(s) 460, 685, 766
LmnI GCTCC 3 cut(s) 20, 250, 809
Lsp1109I GCAGC 2 cut(s) 575, 758
LweI GCATC 2 cut(s) 404, 758
MaeI CTAG 2 cut(s) 140, 560
MaeIII GTNAC 3 cut(s) 277, 533, 670
MalI GATC 3 cut(s) 462, 687, 768
MboI GATC 3 cut(s) 460, 685, 766
MboII GAAGA 2 cut(s) 248, 761
MflI RGATCY 1 cut(s) 766
MluCI AATT 4 cut(s) 402, 479, 795, 945
Mly113I GGCGCC 1 cut(s) 495
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 282
Mph1103I ATGCAT 1 cut(s) 419
MseI TTAA 2 cut(s) 545, 711
MslI CAYNNNNRTG 2 cut(s) 284, 837
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 2 cut(s) 176, 330
MspR9I CCNGG 2 cut(s) 219, 818
MvaI CCWGG 2 cut(s) 219, 818
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 7 cut(s) 65, 431, 440, 464, 572, 631, 755
NarI GGCGCC 1 cut(s) 495
NdeII GATC 3 cut(s) 460, 685, 766
NlaIII CATG 2 cut(s) 570, 836
NlaIV GGNNCC 2 cut(s) 16, 496
NmuCI GTSAC 3 cut(s) 277, 533, 670
NsiI ATGCAT 1 cut(s) 419
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 256
PfeI GAWTC 1 cut(s) 179
PflFI GACNNNGTC 2 cut(s) 140, 152
PkrI GCNGC 2 cut(s) 565, 748
PleI GAGTC 1 cut(s) 169
PluTI GGCGCC 1 cut(s) 498
PpsI GAGTC 1 cut(s) 169
PpuMI RGGWCCY 2 cut(s) 136, 640
Psp5II RGGWCCY 2 cut(s) 136, 640
Psp6I CCWGG 2 cut(s) 217, 816
PspGI CCWGG 2 cut(s) 217, 816
PspN4I GGNNCC 2 cut(s) 16, 496
PspPI GGNCC 2 cut(s) 136, 640
PspPPI RGGWCCY 2 cut(s) 136, 640
PsuI RGATCY 1 cut(s) 766
PsyI GACNNNGTC 2 cut(s) 140, 152
RsaI GTAC 3 cut(s) 342, 512, 595
RsaNI GTAC 3 cut(s) 341, 511, 594
RseI CAYNNNNRTG 2 cut(s) 284, 837
SaqAI TTAA 2 cut(s) 545, 711
SatI GCNGC 2 cut(s) 564, 747
Sau3AI GATC 3 cut(s) 460, 685, 766
Sau96I GGNCC 2 cut(s) 136, 640
ScaI AGTACT 1 cut(s) 595
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 2 cut(s) 219, 818
SfaNI GCATC 2 cut(s) 404, 758
SfoI GGCGCC 1 cut(s) 496
SinI GGWCC 2 cut(s) 136, 640
SmiMI CAYNNNNRTG 2 cut(s) 284, 837
SmlI CTYRAG 2 cut(s) 95, 710
SmoI CTYRAG 2 cut(s) 95, 710
Sse9I AATT 4 cut(s) 402, 479, 795, 945
SsiI CCGC 1 cut(s) 52
SspDI GGCGCC 1 cut(s) 494
SspI AATATT 1 cut(s) 453
SspMI CTAG 2 cut(s) 140, 560
StyD4I CCNGG 2 cut(s) 217, 816
StyI CCWWGG 2 cut(s) 60, 234
TaaI ACNGT 4 cut(s) 8, 376, 515, 955
TaqI TCGA 3 cut(s) 147, 207, 259
TasI AATT 4 cut(s) 402, 479, 795, 945
TatI WGTACW 1 cut(s) 593
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 2 cut(s) 545, 711
Tru9I TTAA 2 cut(s) 545, 711
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 3 cut(s) 277, 533, 670
TseI GCWGC 2 cut(s) 563, 746
Tsp45I GTSAC 3 cut(s) 277, 533, 670
TspDTI ATGAA 2 cut(s) 492, 518
TspGWI ACGGA 1 cut(s) 193
TspRI CASTG 1 cut(s) 379
Tth111I GACNNNGTC 2 cut(s) 140, 152
Vha464I CTTAAG 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 136, 640
XapI RAATTY 2 cut(s) 402, 795
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 2 cut(s) 140, 560
ZrmI AGTACT 1 cut(s) 595
Zsp2I ATGCAT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.