Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0014871

Physical Location & Seq

Genomic Coordinates Reverse (-)

9708641 .. 9776940

Gene Structure 68300 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG054100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1881 bp

ATGAACGAATTGCGACATTCAGTTGGATGCGTGGCCATTTTTTTTTATCATGCTTATGCTATCATAATCTATGAGATGTGCATTGAGGAGCCTACTGCAACTGTCACAAAGGTCAGACAACAGGAGAAGCTGAAGTATCCTCCATATCCTTTGAATACCATTGAGCTTGAAAAGCGTGCGTCAAGATACTTTCGAATGAGTTCTGAACATACTATGAAGGTAGCAGAAGAGCTTTACCAATCTGGTTTCATCAGTTATCCTCGCACAGAGACCGATGGCTTCTCTCCAAATACTGATTTGCATGTTAGATCTCTCATCAAGTTGGTGTATTTTTCTAGACACGAAGACCAATCAATTGATGACAAAAGGAAGAAAACTTTGAAGGAGAGACTGGGAATTTCCAATGGTAGTCGAAGAAGCTACCCTGTTCTTGGTGGAAGGCTTCATTTTGTGAAGTTTGAGACGACCAAGATTAATGAGTGCTTAGACTTCATTTATTCTAAACAACTTCACCGTGGAGGGATGGATGTTCGTATTTGGAATCCTGATGCCCCAACCAATGAAAATGCTGTAATTAAGGCAACAGGTGGTGGAGCTTACAAGTTTGCAGACCTTTTTAAGGAAAGGCTTGGGGGTGATGATATTACTGTGGCAGCTGTAAGGGAAGTTAAAGAAGAAATAGGAGTTGTAATATCTTTCATACTATTTTGCAAATATTGTAGTCATGTAGTCTTAGCCTCTTTAAATTATTGTTGCAAGAATATTGTGCAGCAATGTGTTTCTGCTGGAGGCACAACATTATGCATAGTTGGCTTTTGTAAATTCGCTGCCCCGGTGATAGTTGTGACTATCACTTCCGACGCGGTGGTCGTAGCCTCCCATACCTCCTCCGTTACTAACCAAGATGAAGGCCTTGCAATCTTGTTGTGTCCTTCAGAAGGAGTTGTAAGCTTCTCAGCTTTCTCAGTGCTTTCGGTTTGTCGCAATCATCATCTACTTCAGCTATTTGGCGGCCCACAATGGCTTACCATCAAATGGGGTTCACATTGTTATGTGAAGAAGGTGAAAGAAATGTTGGAGAGTAAAGGGTGTCAGATAAGAACCGGCTGTGAGGTGCATAAAGTTTCCACAACTGATGAGGGTTGCACTGTTTTTACTGGAGATGGTTTTGAGGAAATATACGATGGATGCATTATAGCTATCCAAGCCCCCGATGCCATGAGAATGTTAGGAGAACAGGCAACACCTGATGAACTGAGAATACTTGGCGCCTTCCAATATGTGTACTGTGATATTTTCCTTCATCGTGACAAAACTTTAATGCCCCGAAATCTAGCAGCATGGAGTACATTAAACTATCTTGGAAGTACTGACAACAAAGTATGTTTGACATACTGGCTCAATCAGCTTCAGAACCTCGGTGAAACAAATCAACCATATCTTGTGACACTCAATCCAGATCATACACCGAACCACACCTTGCTTAAGTGGTCAACAAGCCATCCAGTCCTATCTGTTGCTGCATCGAAAGCTTCGCTGGAGCTTCCTCATATTCAAGGAAAGAGAGGAATTTGGTATTGTGGAGCATACCAGGGTTATGGCTTCCATGAGGATGGGCTAAAGTTTTGGGCTGGTATCTCTTTTGGTTTGGGTGGTCGGATCCCAGAGGATTTCGGGTGGTCAGCAACAGGTTCTACACTAGTCTTCTGTATTGACCTGCCGGTTCTATCTTTGATCGAAGGCGGAGGGGTGTGGGGACCAGAGTCGACCTGCGACGTGATACAGATTGAGGTTGTGCGAGCCCGGAGGAGCTTCTACCGAATTCCAGGAAGTGTATTTGAGTGCAGGGGATCGAGTTCTCTTCCTGATTTAGTCAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006629 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0008825 GO:0009987 GO:0016740 GO:0016741 GO:0030258 GO:0032259 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Amino_oxidase CMAS NAD_binding_8

Protein Analysis

626

Amino Acids

69.83

Weight (kDa)

6.68

Isoelectric Point (pI)

38.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Topoisom_bac	PF01131	23 - 112	1.2e-21	DNA topoisomerase
Fumble	PF03630	104 - 222	3.2e-16	Fumble

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014871)

Species	Orthologous Gene IDs
rosa_laevigata	RLG00000016735
rosa_multiflora	Rmu_sc0000545.1_g000012 Rmu_sc0000842.1_g000040 Rmu_sc0012270.1_g000001 Rmu_sc0015438.1_g000001 Rmu_sc0029718.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068330
rosa_samantha	Rh2BG146200 Rh3CG268200 Rh4AG055800 Rh4BG053200 Rh4BG054100 Rh4BG054500 Rh4CG060400 Rh6BG426700 Rh7AG370000 Rh7CG388900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	866
Acc36I	ACCTGC	2 cut(s)	1725, 1778
AccB1I	GGYRCC	1 cut(s)	1268
AccB7I	CCANNNNNTGG	1 cut(s)	1035
AccI	GTMKAC	1 cut(s)	1766
AccII	CGCG	1 cut(s)	863
AciI	CCGC	3 cut(s)	863, 1011, 1743
AclWI	GGATC	3 cut(s)	1654, 1667, 1858
AcoI	YGGCCR	1 cut(s)	33
AcsI	RAATTY	4 cut(s)	396, 821, 1569, 1821
AcuI	CTGAAG	4 cut(s)	152, 918, 983, 1394
AcyI	GRCGYC	1 cut(s)	1269
AfaI	GTAC	3 cut(s)	1286, 1348, 1369
AfiI	CCNNNNNNNGG	4 cut(s)	431, 619, 938, 1035
AflII	CTTAAG	1 cut(s)	1484
AgsI	TTSAA	4 cut(s)	154, 170, 382, 1556
AhlI	ACTAGT	1 cut(s)	1699
AjiI	CACGTC	1 cut(s)	1777
AjnI	CCWGG	2 cut(s)	1590, 1825
AjuI	GAANNNNNNNTTGG	2 cut(s)	1058, 1090
Alw26I	GTCTC	3 cut(s)	263, 382, 455
AlwI	GGATC	3 cut(s)	1654, 1667, 1858
AoxI	GGCC	3 cut(s)	33, 910, 1012
ApeKI	GCWGC	5 cut(s)	653, 769, 827, 1337, 1520
ApoI	RAATTY	4 cut(s)	396, 821, 1569, 1821
AseI	ATTAAT	1 cut(s)	474
Asp700I	GAANNNNTTC	1 cut(s)	199
AspLEI	GCGC	1 cut(s)	1271
AspS9I	GGNCC	2 cut(s)	1013, 1757
AsuC2I	CCSGG	2 cut(s)	833, 1804
AsuHPI	GGTGA	5 cut(s)	503, 647, 847, 1075, 1433
AsuII	TTCGAA	1 cut(s)	193
AvaII	GGWCC	1 cut(s)	1757
BalI	TGGCCA	1 cut(s)	35
BamHI	GGATCC	1 cut(s)	1659
BanI	GGYRCC	1 cut(s)	1268
BanII	GRGCYC	1 cut(s)	1804
BbsI	GAAGAC	2 cut(s)	351, 1696
BbvI	GCAGC	5 cut(s)	665, 781, 814, 1349, 1507
BccI	CCATC	7 cut(s)	269, 517, 1037, 1157, 1178, 1509, 1607
BciT130I	CCWGG	2 cut(s)	1592, 1827
BciVI	GTATCC	1 cut(s)	147
BcnI	CCSGG	2 cut(s)	833, 1804
BcoDI	GTCTC	3 cut(s)	263, 382, 455
BcuI	ACTAGT	1 cut(s)	1699
BfaI	CTAG	3 cut(s)	336, 1334, 1700
BfoI	RGCGCY	1 cut(s)	1272
BfrI	CTTAAG	1 cut(s)	1484
BfuAI	ACCTGC	2 cut(s)	1725, 1778
BfuI	GTATCC	1 cut(s)	147
BglII	AGATCT	1 cut(s)	308
BisI	GCNGC	6 cut(s)	654, 770, 828, 1012, 1338, 1521
BlsI	GCNGC	6 cut(s)	655, 771, 829, 1013, 1339, 1522
BmcAI	AGTACT	1 cut(s)	1369
Bme1390I	CCNGG	4 cut(s)	833, 1592, 1804, 1827
Bme18I	GGWCC	1 cut(s)	1757
BmgBI	CACGTC	1 cut(s)	1777
BmgT120I	GGNCC	2 cut(s)	1013, 1757
BmiI	GGNNCC	4 cut(s)	90, 1270, 1661, 1758
BmrFI	CCNGG	4 cut(s)	833, 1592, 1804, 1827
BmrI	ACTGGG	1 cut(s)	401
BmsI	GCATC	5 cut(s)	17, 538, 1178, 1204, 1532
BmuI	ACTGGG	1 cut(s)	401
BoxI	GACNNNNGTC	1 cut(s)	1762
BpiI	GAAGAC	2 cut(s)	351, 1696
BpmI	CTGGAG	3 cut(s)	807, 1179, 1559
Bpu14I	TTCGAA	1 cut(s)	193
BpuMI	CCSGG	2 cut(s)	833, 1804
BsaHI	GRCGYC	1 cut(s)	1269
BsaI	GGTCTC	1 cut(s)	263
BsaJI	CCNNGG	4 cut(s)	514, 831, 1417, 1591
Bsc4I	CCNNNNNNNGG	4 cut(s)	431, 619, 938, 1035
Bse118I	RCCGGY	2 cut(s)	1103, 1720
Bse1I	ACTGG	4 cut(s)	396, 1162, 1400, 1505
Bse3DI	GCAATG	1 cut(s)	779
BseBI	CCWGG	2 cut(s)	1592, 1827
BseDI	CCNNGG	4 cut(s)	514, 831, 1417, 1591
BseGI	GGATG	6 cut(s)	32, 528, 532, 1193, 1501, 1618
BseLI	CCNNNNNNNGG	4 cut(s)	431, 619, 938, 1035
BseMI	GCAATG	1 cut(s)	779
BseMII	CTCAG	3 cut(s)	969, 978, 1247
BseNI	ACTGG	4 cut(s)	396, 1162, 1400, 1505
BseRI	GAGGAG	3 cut(s)	101, 877, 1822
BseXI	GCAGC	5 cut(s)	665, 781, 814, 1349, 1507
BsgI	GTGCAG	2 cut(s)	788, 1864
Bsh1236I	CGCG	1 cut(s)	863
BshFI	GGCC	3 cut(s)	35, 912, 1014
BshNI	GGYRCC	1 cut(s)	1268
BsiSI	CCGG	4 cut(s)	833, 1104, 1721, 1804
BslFI	GGGAC	1 cut(s)	1770
BslI	CCNNNNNNNGG	4 cut(s)	431, 619, 938, 1035
BsmAI	GTCTC	3 cut(s)	263, 382, 455
BsmBI	CGTCTC	1 cut(s)	455
BsmFI	GGGAC	1 cut(s)	1770
BsnI	GGCC	3 cut(s)	35, 912, 1014
Bso31I	GGTCTC	1 cut(s)	263
Bsp119I	TTCGAA	1 cut(s)	193
Bsp1286I	GDGCHC	1 cut(s)	1804
Bsp143I	GATC	5 cut(s)	308, 1459, 1659, 1734, 1850
BspACI	CCGC	3 cut(s)	863, 1011, 1743
BspANI	GGCC	3 cut(s)	35, 912, 1014
BspCNI	CTCAG	3 cut(s)	968, 977, 1248
BspFNI	CGCG	1 cut(s)	863
BspLI	GGNNCC	4 cut(s)	90, 1270, 1661, 1758
BspMI	ACCTGC	2 cut(s)	1725, 1778
BspPI	GGATC	3 cut(s)	1654, 1667, 1858
BspQI	GCTCTTC	1 cut(s)	222
BspT104I	TTCGAA	1 cut(s)	193
BspT107I	GGYRCC	1 cut(s)	1268
BspTI	CTTAAG	1 cut(s)	1484
BspTNI	GGTCTC	1 cut(s)	263
BsrDI	GCAATG	1 cut(s)	779
BsrFI	RCCGGY	2 cut(s)	1103, 1720
BsrI	ACTGG	4 cut(s)	396, 1162, 1400, 1505
BssAI	RCCGGY	2 cut(s)	1103, 1720
BssECI	CCNNGG	4 cut(s)	514, 831, 1417, 1591
BssMI	GATC	5 cut(s)	308, 1459, 1659, 1734, 1850
BssNI	GRCGYC	1 cut(s)	1269
Bst2UI	CCWGG	2 cut(s)	1592, 1827
Bst4CI	ACNGT	5 cut(s)	103, 515, 649, 1150, 1289
Bst6I	CTCTTC	2 cut(s)	222, 1866
BstACI	GRCGYC	1 cut(s)	1269
BstAFI	CTTAAG	1 cut(s)	1484
BstBI	TTCGAA	1 cut(s)	193
BstC8I	GCNNGC	2 cut(s)	177, 1800
BstDEI	CTNAG	5 cut(s)	484, 733, 955, 964, 1256
BstDSI	CCRYGG	1 cut(s)	514
BstENI	CCTNNNNNAGG	2 cut(s)	617, 936
BstF5I	GGATG	6 cut(s)	32, 528, 532, 1193, 1501, 1618
BstFNI	CGCG	1 cut(s)	863
BstH2I	RGCGCY	1 cut(s)	1272
BstHHI	GCGC	1 cut(s)	1271
BstKTI	GATC	5 cut(s)	311, 1462, 1662, 1737, 1853
BstMAI	GTCTC	3 cut(s)	263, 382, 455
BstMBI	GATC	5 cut(s)	308, 1459, 1659, 1734, 1850
BstMWI	GCNNNNNNNGC	6 cut(s)	172, 810, 1205, 1214, 1405, 1529
BstNI	CCWGG	2 cut(s)	1592, 1827
BstNSI	RCATGY	1 cut(s)	305
BstPAI	GACNNNNGTC	1 cut(s)	1762
BstSCI	CCNGG	4 cut(s)	831, 1590, 1802, 1825
BstUI	CGCG	1 cut(s)	863
BstV1I	GCAGC	5 cut(s)	665, 781, 814, 1349, 1507
BstV2I	GAAGAC	2 cut(s)	351, 1696
BstX2I	RGATCY	2 cut(s)	308, 1659
BstXI	CCANNNNNNTGG	2 cut(s)	1598, 1613
BstYI	RGATCY	2 cut(s)	308, 1659
BsuI	GTATCC	1 cut(s)	147
BsuRI	GGCC	3 cut(s)	35, 912, 1014
BtgI	CCRYGG	1 cut(s)	514
BtrI	CACGTC	1 cut(s)	1777
BtsCI	GGATG	6 cut(s)	32, 528, 532, 1193, 1501, 1618
BtsIMutI	CAGTG	2 cut(s)	972, 1146
BveI	ACCTGC	2 cut(s)	1725, 1778
Cac8I	GCNNGC	2 cut(s)	177, 1800
CfoI	GCGC	1 cut(s)	1271
Cfr10I	RCCGGY	2 cut(s)	1103, 1720
Cfr13I	GGNCC	2 cut(s)	1013, 1757
CseI	GACGC	2 cut(s)	168, 869
Csp6I	GTAC	3 cut(s)	1285, 1347, 1368
CviAII	CATG	6 cut(s)	50, 302, 725, 1219, 1341, 1607
CviQI	GTAC	3 cut(s)	1285, 1347, 1368
DdeI	CTNAG	5 cut(s)	484, 733, 955, 964, 1256
DinI	GGCGCC	1 cut(s)	1270
DpnI	GATC	5 cut(s)	310, 1461, 1661, 1736, 1852
DpnII	GATC	5 cut(s)	308, 1459, 1659, 1734, 1850
DraI	TTTAAA	1 cut(s)	744
DrdI	GACNNNNNNGTC	1 cut(s)	866
DseDI	GACNNNNNNGTC	1 cut(s)	866
EaeI	YGGCCR	1 cut(s)	33
Eam1104I	CTCTTC	2 cut(s)	222, 1866
EarI	CTCTTC	2 cut(s)	222, 1866
EciI	GGCGGA	1 cut(s)	1758
Eco147I	AGGCCT	1 cut(s)	912
Eco24I	GRGCYC	1 cut(s)	1804
Eco31I	GGTCTC	1 cut(s)	263
Eco47I	GGWCC	1 cut(s)	1757
Eco57I	CTGAAG	4 cut(s)	152, 918, 983, 1394
EcoNI	CCTNNNNNAGG	2 cut(s)	617, 936
EcoRI	GAATTC	1 cut(s)	1821
EcoRII	CCWGG	2 cut(s)	1590, 1825
EcoT22I	ATGCAT	2 cut(s)	806, 1193
EcoT38I	GRGCYC	1 cut(s)	1804
EgeI	GGCGCC	1 cut(s)	1270
EheI	GGCGCC	1 cut(s)	1270
Esp3I	CGTCTC	1 cut(s)	455
FaeI	CATG	6 cut(s)	53, 305, 728, 1222, 1344, 1610
FaqI	GGGAC	1 cut(s)	1770
FatI	CATG	6 cut(s)	49, 301, 724, 1218, 1340, 1606
FblI	GTMKAC	1 cut(s)	1766
Fnu4HI	GCNGC	6 cut(s)	654, 770, 828, 1012, 1338, 1521
FokI	GGATG	6 cut(s)	39, 535, 539, 1200, 1488, 1625
FriOI	GRGCYC	1 cut(s)	1804
Fsp4HI	GCNGC	6 cut(s)	654, 770, 828, 1012, 1338, 1521
FspBI	CTAG	3 cut(s)	336, 1334, 1700
GlaI	GCGC	1 cut(s)	1270
GluI	GCNGC	6 cut(s)	654, 770, 828, 1012, 1338, 1521
GsuI	CTGGAG	3 cut(s)	807, 1179, 1559
HaeII	RGCGCY	1 cut(s)	1272
HaeIII	GGCC	3 cut(s)	35, 912, 1014
HapII	CCGG	4 cut(s)	833, 1104, 1721, 1804
HgaI	GACGC	2 cut(s)	168, 869
HhaI	GCGC	1 cut(s)	1271
Hin1I	GRCGYC	1 cut(s)	1269
Hin1II	CATG	6 cut(s)	53, 305, 728, 1222, 1344, 1610
Hin6I	GCGC	1 cut(s)	1269
HinP1I	GCGC	1 cut(s)	1269
HincII	GTYRAC	2 cut(s)	1494, 1767
HindII	GTYRAC	2 cut(s)	1494, 1767
HindIII	AAGCTT	2 cut(s)	949, 1530
HinfI	GANTC	2 cut(s)	541, 1763
HpaII	CCGG	4 cut(s)	833, 1104, 1721, 1804
HphI	GGTGA	5 cut(s)	503, 647, 847, 1075, 1433
Hpy166II	GTNNAC	4 cut(s)	1043, 1285, 1494, 1767
Hpy188I	TCNGA	7 cut(s)	116, 205, 859, 937, 1095, 1413, 1659
Hpy188III	TCNNGA	6 cut(s)	183, 336, 545, 1307, 1457, 1865
Hpy8I	GTNNAC	4 cut(s)	1043, 1285, 1494, 1767
Hpy99I	CGWCG	2 cut(s)	863, 1778
HpyCH4III	ACNGT	5 cut(s)	103, 515, 649, 1150, 1289
HpyCH4IV	ACGT	1 cut(s)	1776
HpyF10VI	GCNNNNNNNGC	6 cut(s)	172, 810, 1205, 1214, 1405, 1529
HpyF3I	CTNAG	5 cut(s)	484, 733, 955, 964, 1256
HpySE526I	ACGT	1 cut(s)	1776
Hsp92I	GRCGYC	1 cut(s)	1269
Hsp92II	CATG	6 cut(s)	53, 305, 728, 1222, 1344, 1610
HspAI	GCGC	1 cut(s)	1269
KasI	GGCGCC	1 cut(s)	1268
Kzo9I	GATC	5 cut(s)	308, 1459, 1659, 1734, 1850
LguI	GCTCTTC	1 cut(s)	222
LmnI	GCTCC	5 cut(s)	88, 593, 1540, 1583, 1809
Lsp1109I	GCAGC	5 cut(s)	665, 781, 814, 1349, 1507
LweI	GCATC	5 cut(s)	17, 538, 1178, 1204, 1532
MaeI	CTAG	3 cut(s)	336, 1334, 1700
MaeII	ACGT	1 cut(s)	1776
MaeIII	GTNAC	5 cut(s)	103, 844, 892, 1307, 1444
MalI	GATC	5 cut(s)	310, 1461, 1661, 1736, 1852
MboI	GATC	5 cut(s)	308, 1459, 1659, 1734, 1850
MboII	GAAGA	8 cut(s)	239, 356, 382, 426, 686, 1069, 1696, 1853
MfeI	CAATTG	1 cut(s)	354
MflI	RGATCY	2 cut(s)	308, 1659
MhlI	GDGCHC	1 cut(s)	1804
MlsI	TGGCCA	1 cut(s)	35
MluCI	AATT	9 cut(s)	8, 354, 396, 573, 745, 821, 1569, 1821, 1876
MluNI	TGGCCA	1 cut(s)	35
Mly113I	GGCGCC	1 cut(s)	1269
MlyI	GAGTC	1 cut(s)	1772
MmeI	TCCRAC	4 cut(s)	4, 882, 1056, 1637
Mox20I	TGGCCA	1 cut(s)	35
Mph1103I	ATGCAT	2 cut(s)	806, 1193
MroXI	GAANNNNTTC	1 cut(s)	199
MscI	TGGCCA	1 cut(s)	35
MseI	TTAA	8 cut(s)	474, 576, 618, 669, 743, 1319, 1352, 1485
MslI	CAYNNNNRTG	4 cut(s)	54, 1050, 1223, 1611
Msp20I	TGGCCA	1 cut(s)	35
MspA1I	CMGCKG	1 cut(s)	656
MspCI	CTTAAG	1 cut(s)	1484
MspI	CCGG	4 cut(s)	833, 1104, 1721, 1804
MspR9I	CCNGG	4 cut(s)	833, 1592, 1804, 1827
MunI	CAATTG	1 cut(s)	354
MvaI	CCWGG	2 cut(s)	1592, 1827
MvnI	CGCG	1 cut(s)	863
MwoI	GCNNNNNNNGC	6 cut(s)	172, 810, 1205, 1214, 1405, 1529
NarI	GGCGCC	1 cut(s)	1269
NciI	CCSGG	2 cut(s)	833, 1804
NdeII	GATC	5 cut(s)	308, 1459, 1659, 1734, 1850
NlaIII	CATG	6 cut(s)	53, 305, 728, 1222, 1344, 1610
NlaIV	GGNNCC	4 cut(s)	90, 1270, 1661, 1758
NmuCI	GTSAC	4 cut(s)	103, 844, 1307, 1444
NsiI	ATGCAT	2 cut(s)	806, 1193
NspI	RCATGY	1 cut(s)	305
NspV	TTCGAA	1 cut(s)	193
PceI	AGGCCT	1 cut(s)	912
PciSI	GCTCTTC	1 cut(s)	222
PcsI	WCGNNNNNNNCGW	1 cut(s)	867
PdmI	GAANNNNTTC	1 cut(s)	199
PfeI	GAWTC	1 cut(s)	541
PflMI	CCANNNNNTGG	1 cut(s)	1035
PfoI	TCCNGGA	1 cut(s)	1825
PkrI	GCNGC	6 cut(s)	655, 771, 829, 1013, 1339, 1522
PleI	GAGTC	1 cut(s)	1771
PluTI	GGCGCC	1 cut(s)	1272
PpsI	GAGTC	1 cut(s)	1771
PshAI	GACNNNNGTC	1 cut(s)	1762
PshBI	ATTAAT	1 cut(s)	474
Psp6I	CCWGG	2 cut(s)	1590, 1825
PspGI	CCWGG	2 cut(s)	1590, 1825
PspN4I	GGNNCC	4 cut(s)	90, 1270, 1661, 1758
PspPI	GGNCC	2 cut(s)	1013, 1757
PsrI	GAACNNNNNNTAC	2 cut(s)	1245, 1277
PsuI	RGATCY	2 cut(s)	308, 1659
PvuII	CAGCTG	1 cut(s)	656
RsaI	GTAC	3 cut(s)	1286, 1348, 1369
RsaNI	GTAC	3 cut(s)	1285, 1347, 1368
RseI	CAYNNNNRTG	4 cut(s)	54, 1050, 1223, 1611
SalI	GTCGAC	1 cut(s)	1765
SapI	GCTCTTC	1 cut(s)	222
SaqAI	TTAA	8 cut(s)	474, 576, 618, 669, 743, 1319, 1352, 1485
SatI	GCNGC	6 cut(s)	654, 770, 828, 1012, 1338, 1521
Sau3AI	GATC	5 cut(s)	308, 1459, 1659, 1734, 1850
Sau96I	GGNCC	2 cut(s)	1013, 1757
ScaI	AGTACT	1 cut(s)	1369
SchI	GAGTC	1 cut(s)	1772
ScrFI	CCNGG	4 cut(s)	833, 1592, 1804, 1827
SduI	GDGCHC	1 cut(s)	1804
SfaNI	GCATC	5 cut(s)	17, 538, 1178, 1204, 1532
SfoI	GGCGCC	1 cut(s)	1270
SfuI	TTCGAA	1 cut(s)	193
SinI	GGWCC	1 cut(s)	1757
SmiMI	CAYNNNNRTG	4 cut(s)	54, 1050, 1223, 1611
SmlI	CTYRAG	1 cut(s)	1484
SmoI	CTYRAG	1 cut(s)	1484
SpeI	ACTAGT	1 cut(s)	1699
Sse9I	AATT	9 cut(s)	8, 354, 396, 573, 745, 821, 1569, 1821, 1876
SseBI	AGGCCT	1 cut(s)	912
SsiI	CCGC	3 cut(s)	863, 1011, 1743
SspDI	GGCGCC	1 cut(s)	1268
SspI	AATATT	2 cut(s)	716, 763
SspMI	CTAG	3 cut(s)	336, 1334, 1700
StuI	AGGCCT	1 cut(s)	912
StyD4I	CCNGG	4 cut(s)	831, 1590, 1802, 1825
TaaI	ACNGT	5 cut(s)	103, 515, 649, 1150, 1289
TaiI	ACGT	1 cut(s)	1779
TaqI	TCGA	6 cut(s)	193, 412, 1526, 1737, 1766, 1853
TaqII	GACCGA	1 cut(s)	287
TasI	AATT	9 cut(s)	8, 354, 396, 573, 745, 821, 1569, 1821, 1876
TatI	WGTACW	3 cut(s)	1284, 1346, 1367
TauI	GCSGC	1 cut(s)	1014
TfiI	GAWTC	1 cut(s)	541
Tru1I	TTAA	8 cut(s)	474, 576, 618, 669, 743, 1319, 1352, 1485
Tru9I	TTAA	8 cut(s)	474, 576, 618, 669, 743, 1319, 1352, 1485
TscAI	CASTG	2 cut(s)	972, 1153
TseFI	GTSAC	4 cut(s)	103, 844, 1307, 1444
TseI	GCWGC	5 cut(s)	653, 769, 827, 1337, 1520
Tsp45I	GTSAC	4 cut(s)	103, 844, 1307, 1444
TspGWI	ACGGA	1 cut(s)	880
TspRI	CASTG	2 cut(s)	972, 1153
Van91I	CCANNNNNTGG	1 cut(s)	1035
Vha464I	CTTAAG	1 cut(s)	1484
VpaK11BI	GGWCC	1 cut(s)	1757
VspI	ATTAAT	1 cut(s)	474
XagI	CCTNNNNNAGG	2 cut(s)	617, 936
XapI	RAATTY	4 cut(s)	396, 821, 1569, 1821
XbaI	TCTAGA	1 cut(s)	335
XceI	RCATGY	1 cut(s)	305
XmiI	GTMKAC	1 cut(s)	1766
XmnI	GAANNNNTTC	1 cut(s)	199
XspI	CTAG	3 cut(s)	336, 1334, 1700
ZrmI	AGTACT	1 cut(s)	1369
Zsp2I	ATGCAT	2 cut(s)	806, 1193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.