RLG00000019399

Arogenate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012529
Physical Location & Seq
Reverse (-)
51530131 .. 51531774
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019399

Sequence Viewer

Length: 1179 bp
ATGCTCTCATTCTCTTCCACTCAAACCAATCCTTCACTCCTCCCTCCAAATCTCTACCCATCTTCTCACTCATTCTCTCACTCTCTTCTCCCACCTCTAAAACCCCACAAAAACCAGACCCAACTTCATCTCCAACGCCTAACAATCCAAGCCATCGATGCTGCACAGCCATTTGACTACGAGTTCCAACTCAAAACCCACTACCAAAAATCCAATGCTCTCAAAATTGGCATCATTGGCTTCGGCAACTACGGCCAGTTCTTGGCCAGGTCCTTCGTCCAACAAGGTCACACTGTCCTCGCCCATTCTCGCACTGACTACTCCAAACTCGCCCAAGACGTCGGCGTTTCGTTCTTGACTGACCCCAATGACCTCTGTGAGCAACACCCAGAAGTGGTTCTTTTATGTACTTCGATTATGTCCAGTGAAAAGGTAATCAAAACCTTCCCGTTTCAGAGACTTCTTCGAAATACCTTATTTGTTGATGTTCTTTCAGTAAAGGAGTTCCCAAGAGATTTGTTCCTCAATTACTTGCCTGCTGAGTTTGACATTCTTTGCACACACCCAATGTTTGGTCCACAGAGTGGTAAGGACTCGTGGGTTGGTCTTCCTTTTGTGTATGACAAGGTTAGGATTGGGGATTGTGGTTTTAGGCTCAATAGGTGTGAGAAGTTTTTGGACATTTTCGCAAGGGAGGGGTGTAGAATGGTGGAAATGTCGTGTGCCGAGCACGATAGGGATGCTGCAGGGTCACAATTTGTGACACACACAATGGGGCGAGTGTTGGAGAAATTCAGATTGGAGTCCTCGCCGATTAATACTAAAGGGTATGAGACTTTGTTGAATTTGGTGGAGAATACGGCTGGGGATAGCTTTGAGTTGTACTATGGATTGTTTATGTATAACAAGAATGCAATGGAGCAGCTGGAGAGGCTGGACATGGCATTCGAGGATCTTAAGAAAGAGTTGTTTGGGCATTTGCATGAGGTTTGTAGGAGACAGTTGTTTGGGAGCGCTGAAGGGGTCGAAGCCTTGAGAGAAGAAGAGGATGCAGAACCGCAGAAGTTGCTTTCGACTGGTAGTCAAATCGGAAGCAATGAGGATCCTCGGAATGGTGCTCAAAAAGGTTACGAAAACGGTCATGCTTCTCTGAATTCAGCATCTGTGTATCTAAGATAA

Protein Analysis

393

Amino Acids

44.44

Weight (kDa)

5.88

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 59 - 162 2.3e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
F420_oxidored PF03807 75 - 164 7.7e-11 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 95 - 198 1.1e-06 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 261 - 334 4e-35 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 342
AccB7I CCANNNNNTGG 3 cut(s) 262, 572, 584
AciI CCGC 1 cut(s) 1058
AclWI GGATC 3 cut(s) 960, 1097, 1110
AcoI YGGCCR 2 cut(s) 253, 264
AcsI RAATTY 3 cut(s) 791, 844, 1153
AcuI CTGAAG 1 cut(s) 1038
AcyI GRCGYC 1 cut(s) 339
AdeI CACNNNGTG 1 cut(s) 584
AfaI GTAC 2 cut(s) 409, 884
AfeI AGCGCT 1 cut(s) 1015
AfiI CCNNNNNNNGG 5 cut(s) 262, 394, 572, 584, 1112
AflII CTTAAG 1 cut(s) 956
AgsI TTSAA 1 cut(s) 844
AhdI GACNNNNNGTC 1 cut(s) 1080
AjnI CCWGG 1 cut(s) 266
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
AluBI AGCT 2 cut(s) 873, 925
AluI AGCT 2 cut(s) 873, 925
Alw21I GWGCWC 2 cut(s) 732, 1120
Alw26I GTCTC 3 cut(s) 451, 827, 991
AlwI GGATC 3 cut(s) 960, 1097, 1110
AlwNI CAGNNNCTG 1 cut(s) 1163
Aor51HI AGCGCT 1 cut(s) 1015
AoxI GGCC 2 cut(s) 253, 264
ApeKI GCWGC 3 cut(s) 161, 743, 922
ApoI RAATTY 3 cut(s) 791, 844, 1153
ArsI GACNNNNNNTTYG 2 cut(s) 929, 961
AseI ATTAAT 1 cut(s) 816
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 1 cut(s) 1016
AspS9I GGNCC 2 cut(s) 270, 575
AsuII TTCGAA 1 cut(s) 466
AvaII GGWCC 2 cut(s) 270, 575
BalI TGGCCA 1 cut(s) 266
BamHI GGATCC 1 cut(s) 1102
BauI CACGAG 1 cut(s) 595
BbsI GAAGAC 1 cut(s) 599
Bbv12I GWGCWC 2 cut(s) 732, 1120
BbvI GCAGC 3 cut(s) 148, 730, 934
BccI CCATC 2 cut(s) 67, 161
BceAI ACGGC 2 cut(s) 268, 876
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 1 cut(s) 268
BcoDI GTCTC 3 cut(s) 451, 827, 991
BfmI CTRYAG 1 cut(s) 744
BfoI RGCGCY 1 cut(s) 1017
BfrI CTTAAG 1 cut(s) 956
BisI GCNGC 3 cut(s) 162, 744, 923
BlsI GCNGC 3 cut(s) 163, 745, 924
Bme1390I CCNGG 1 cut(s) 268
Bme18I GGWCC 2 cut(s) 270, 575
BmeRI GACNNNNNGTC 1 cut(s) 1080
BmgT120I GGNCC 2 cut(s) 270, 575
BmiI GGNNCC 1 cut(s) 1104
BmrFI CCNGG 1 cut(s) 268
BmsI GCATC 5 cut(s) 148, 240, 730, 1039, 1169
BpiI GAAGAC 1 cut(s) 599
BpmI CTGGAG 1 cut(s) 947
Bpu14I TTCGAA 1 cut(s) 466
BpuEI CTTGAG 1 cut(s) 1054
Bsa29I ATCGAT 1 cut(s) 156
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 1 cut(s) 1106
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 5 cut(s) 262, 394, 572, 584, 1112
Bse1I ACTGG 3 cut(s) 256, 423, 1081
Bse3DI GCAATG 2 cut(s) 921, 1102
BseBI CCWGG 1 cut(s) 268
BseCI ATCGAT 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 1106
BseGI GGATG 2 cut(s) 745, 1054
BseLI CCNNNNNNNGG 5 cut(s) 262, 394, 572, 584, 1112
BseMI GCAATG 2 cut(s) 921, 1102
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 3 cut(s) 256, 423, 1081
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 3 cut(s) 148, 730, 934
BseYI CCCAGC 1 cut(s) 863
BsgI GTGCAG 1 cut(s) 147
BshFI GGCC 2 cut(s) 255, 266
BshVI ATCGAT 1 cut(s) 156
BsiHKAI GWGCWC 2 cut(s) 732, 1120
BslI CCNNNNNNNGG 5 cut(s) 262, 394, 572, 584, 1112
BsmAI GTCTC 3 cut(s) 451, 827, 991
BsmI GAATGC 2 cut(s) 916, 944
BsnI GGCC 2 cut(s) 255, 266
Bsp119I TTCGAA 1 cut(s) 466
Bsp1286I GDGCHC 2 cut(s) 732, 1120
Bsp143I GATC 2 cut(s) 952, 1102
BspACI CCGC 1 cut(s) 1058
BspANI GGCC 2 cut(s) 255, 266
BspCNI CTCAG 1 cut(s) 532
BspDI ATCGAT 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 1104
BspMAI CTGCAG 1 cut(s) 748
BspPI GGATC 3 cut(s) 960, 1097, 1110
BspT104I TTCGAA 1 cut(s) 466
BspTI CTTAAG 1 cut(s) 956
BsrDI GCAATG 2 cut(s) 921, 1102
BsrI ACTGG 3 cut(s) 256, 423, 1081
BssECI CCNNGG 1 cut(s) 1106
BssMI GATC 2 cut(s) 952, 1102
BssNI GRCGYC 1 cut(s) 339
BssSI CACGAG 1 cut(s) 595
Bst2BI CACGAG 1 cut(s) 595
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 3 cut(s) 295, 1002, 1139
Bst6I CTCTTC 3 cut(s) 19, 90, 1038
BstACI GRCGYC 1 cut(s) 339
BstAFI CTTAAG 1 cut(s) 956
BstAPI GCANNNNNTGC 1 cut(s) 1066
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 2 cut(s) 540, 1172
BstF5I GGATG 2 cut(s) 745, 1054
BstH2I RGCGCY 1 cut(s) 1017
BstHHI GCGC 1 cut(s) 1016
BstKTI GATC 2 cut(s) 955, 1105
BstMAI GTCTC 3 cut(s) 451, 827, 991
BstMBI GATC 2 cut(s) 952, 1102
BstMWI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 931, 1066
BstNI CCWGG 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 266
BstSFI CTRYAG 1 cut(s) 744
BstV1I GCAGC 3 cut(s) 148, 730, 934
BstV2I GAAGAC 1 cut(s) 599
BstX2I RGATCY 2 cut(s) 952, 1102
BstYI RGATCY 2 cut(s) 952, 1102
Bsu15I ATCGAT 1 cut(s) 156
BsuRI GGCC 2 cut(s) 255, 266
BsuTUI ATCGAT 1 cut(s) 156
BtsCI GGATG 2 cut(s) 745, 1054
BtsIMutI CAGTG 3 cut(s) 291, 312, 430
Cac8I GCNNGC 1 cut(s) 537
CaiI CAGNNNCTG 1 cut(s) 1163
CfoI GCGC 1 cut(s) 1016
Cfr13I GGNCC 2 cut(s) 270, 575
ClaI ATCGAT 1 cut(s) 156
Csp6I GTAC 2 cut(s) 408, 883
CviAII CATG 3 cut(s) 940, 983, 1142
CviQI GTAC 2 cut(s) 408, 883
DdeI CTNAG 2 cut(s) 540, 1172
DpnI GATC 2 cut(s) 954, 1104
DpnII GATC 2 cut(s) 952, 1102
DraIII CACNNNGTG 1 cut(s) 584
DriI GACNNNNNGTC 1 cut(s) 1080
EaeI YGGCCR 2 cut(s) 253, 264
Eam1104I CTCTTC 3 cut(s) 19, 90, 1038
Eam1105I GACNNNNNGTC 1 cut(s) 1080
EarI CTCTTC 3 cut(s) 19, 90, 1038
Eco47I GGWCC 2 cut(s) 270, 575
Eco47III AGCGCT 1 cut(s) 1015
Eco57I CTGAAG 1 cut(s) 1038
EcoO109I RGGNCCY 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 1153
EcoRII CCWGG 1 cut(s) 266
FaeI CATG 3 cut(s) 943, 986, 1145
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FatI CATG 3 cut(s) 939, 982, 1141
Fnu4HI GCNGC 3 cut(s) 162, 744, 923
FokI GGATG 2 cut(s) 752, 1061
Fsp4HI GCNGC 3 cut(s) 162, 744, 923
GlaI GCGC 1 cut(s) 1015
GluI GCNGC 3 cut(s) 162, 744, 923
GsaI CCCAGC 1 cut(s) 867
GsuI CTGGAG 1 cut(s) 947
HaeII RGCGCY 1 cut(s) 1017
HaeIII GGCC 2 cut(s) 255, 266
HhaI GCGC 1 cut(s) 1016
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 3 cut(s) 943, 986, 1145
Hin6I GCGC 1 cut(s) 1014
HinP1I GCGC 1 cut(s) 1014
HinfI GANTC 2 cut(s) 593, 803
Hpy166II GTNNAC 1 cut(s) 578
Hpy188I TCNGA 5 cut(s) 456, 797, 1091, 1110, 1152
Hpy188III TCNNGA 1 cut(s) 355
Hpy8I GTNNAC 1 cut(s) 578
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 4 cut(s) 42, 283, 454, 1013
HpyCH4III ACNGT 3 cut(s) 295, 1002, 1139
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 6 cut(s) 164, 558, 746, 914, 982, 1052
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 931, 1066
HpyF3I CTNAG 2 cut(s) 540, 1172
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 3 cut(s) 943, 986, 1145
HspAI GCGC 1 cut(s) 1014
Kzo9I GATC 2 cut(s) 952, 1102
LmnI GCTCC 2 cut(s) 919, 1011
Lsp1109I GCAGC 3 cut(s) 148, 730, 934
LweI GCATC 5 cut(s) 148, 240, 730, 1039, 1169
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 4 cut(s) 287, 750, 760, 1127
MalI GATC 2 cut(s) 954, 1104
MboI GATC 2 cut(s) 952, 1102
MboII GAAGA 7 cut(s) 6, 54, 77, 455, 599, 1052, 1055
MflI RGATCY 2 cut(s) 952, 1102
MhlI GDGCHC 2 cut(s) 732, 1120
MlsI TGGCCA 1 cut(s) 266
MluCI AATT 6 cut(s) 225, 526, 755, 791, 844, 1153
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 2 cut(s) 587, 812
MmeI TCCRAC 4 cut(s) 157, 211, 304, 765
Mox20I TGGCCA 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 396
MscI TGGCCA 1 cut(s) 266
MseI TTAA 2 cut(s) 816, 957
MslI CAYNNNNRTG 1 cut(s) 981
Msp20I TGGCCA 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 925
MspCI CTTAAG 1 cut(s) 956
MspR9I CCNGG 1 cut(s) 268
Mva1269I GAATGC 2 cut(s) 916, 944
MvaI CCWGG 1 cut(s) 268
MwoI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 931, 1066
NdeII GATC 2 cut(s) 952, 1102
NlaIII CATG 3 cut(s) 943, 986, 1145
NlaIV GGNNCC 1 cut(s) 1104
NmeAIII GCCGAG 1 cut(s) 751
NmuCI GTSAC 3 cut(s) 287, 750, 760
NspV TTCGAA 1 cut(s) 466
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PctI GAATGC 2 cut(s) 916, 944
PdmI GAANNNNTTC 1 cut(s) 396
PflMI CCANNNNNTGG 3 cut(s) 262, 572, 584
PkrI GCNGC 3 cut(s) 163, 745, 924
PleI GAGTC 2 cut(s) 587, 811
PpsI GAGTC 2 cut(s) 587, 811
PpuMI RGGWCCY 1 cut(s) 270
PshBI ATTAAT 1 cut(s) 816
Psp5II RGGWCCY 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 266
PspFI CCCAGC 1 cut(s) 863
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 1 cut(s) 1104
PspPI GGNCC 2 cut(s) 270, 575
PspPPI RGGWCCY 1 cut(s) 270
PstI CTGCAG 1 cut(s) 748
PstNI CAGNNNCTG 1 cut(s) 1163
PsuI RGATCY 2 cut(s) 952, 1102
PvuII CAGCTG 1 cut(s) 925
RsaI GTAC 2 cut(s) 409, 884
RsaNI GTAC 2 cut(s) 408, 883
RseI CAYNNNNRTG 1 cut(s) 981
SaqAI TTAA 2 cut(s) 816, 957
SatI GCNGC 3 cut(s) 162, 744, 923
Sau3AI GATC 2 cut(s) 952, 1102
Sau96I GGNCC 2 cut(s) 270, 575
SchI GAGTC 2 cut(s) 587, 812
ScrFI CCNGG 1 cut(s) 268
SduI GDGCHC 2 cut(s) 732, 1120
SfaNI GCATC 5 cut(s) 148, 240, 730, 1039, 1169
SfcI CTRYAG 1 cut(s) 744
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 2 cut(s) 270, 575
SmiMI CAYNNNNRTG 1 cut(s) 981
SmlI CTYRAG 2 cut(s) 956, 1033
SmoI CTYRAG 2 cut(s) 956, 1033
Sse9I AATT 6 cut(s) 225, 526, 755, 791, 844, 1153
SsiI CCGC 1 cut(s) 1058
StyD4I CCNGG 1 cut(s) 266
TaaI ACNGT 3 cut(s) 295, 1002, 1139
TaiI ACGT 1 cut(s) 342
TaqI TCGA 6 cut(s) 156, 413, 466, 948, 1026, 1073
TasI AATT 6 cut(s) 225, 526, 755, 791, 844, 1153
TatI WGTACW 2 cut(s) 407, 882
Tru1I TTAA 2 cut(s) 816, 957
Tru9I TTAA 2 cut(s) 816, 957
TscAI CASTG 3 cut(s) 298, 319, 430
TseFI GTSAC 3 cut(s) 287, 750, 760
TseI GCWGC 3 cut(s) 161, 743, 922
Tsp45I GTSAC 3 cut(s) 287, 750, 760
TspDTI ATGAA 1 cut(s) 116
TspRI CASTG 3 cut(s) 298, 319, 430
Van91I CCANNNNNTGG 3 cut(s) 262, 572, 584
Vha464I CTTAAG 1 cut(s) 956
VpaK11BI GGWCC 2 cut(s) 270, 575
VspI ATTAAT 1 cut(s) 816
XapI RAATTY 3 cut(s) 791, 844, 1153
XmnI GAANNNNTTC 1 cut(s) 396
ZraI GACGTC 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.