Rh2AG375300

Arogenate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0012529
Physical Location & Seq
Forward (+)
56118849 .. 56120287
1439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG375300.1

Sequence Viewer

Length: 1173 bp
ATGCTCTCATTCTCTTCCACTCAAACCAAACCTTCACTCCTCCCTCCAAATCTCTACCCATCTTCTCACTCATTCTCTCACTCTCTTCTCCCACCTCTAAAACCCGACAAAAACGGGACCCATCTTCATCTCCAACGCCTAACAATCCAAGCCATCGACGCTGCACAGCCCTTTGACTACGAGTCTCAACTCAAAACCCACTACCAAAAATCCAATGCTCTCAAAATTGGCATTATTGGCTTCGGCAACTACGGCCAGTTCTTGGCCAGGTCCTTCGTCCAACAAGGTCACACTGTCCTCGCCCATTCTCGTTCTGACTACTCCAAACTCGCCCAAGAAGTCGGCGTTTCGTTCTTTACCGACCCCAATGACCTCTGTGAGCAACACCCAGAAGTGGTTCTCTTATGTACTTCGATTATGTCCAGTGAAAAGGTAATCAAAACCTTCCCATTTCAGAGACTTCTTCGAAATACCTTATTTGTTGATGTTCTTTCAGTAAAGGAGTTCCCAAGAGATTTGTTCCTCAATTACTTGCCTGCTGAGTTTGACATTCTTTGCACACACCCAATGTTTGGTCCACAGAGTGGTAAGGACTCGTGGGTTGGTCTTCCTTTTGTGTATGACAAGGTTAGGATTGGGGATTGTGGTTTTAGGCTCAATAGGTGTGAGAAGTTTTTGGACATTTTCGCAAGGGAAGGGTGTAGAATGGTGGAAATGTCGTGTGCTGAGCACGATAGGGATGCTGCAGGGTCACAATTTGTGACACACACAATGGGGAGAGTGTTGGAGAAATTCAGATTGGAGTCCTCGCCGATTAATACTAAAGGGTATGAGACTTTGTTGAACTTGGTGGAGAATACGGCTGGGGATAGCTTTGAGTTGTACTATGGGTTGTTTATGTATAACAAGAATGCAATGGAGCAGCTGGAGAGGCTGGACATGGCGTTCGAGGATCTTAAGAAAGAGTTGTTTGGGAATTTGCATGAGGTTTGTAGGAGACAGTTGTTTGGGAGTGATGAACGGGTCGAAGCCGCAGAAGAGGATGCAGAACCGCAGAAGTTGCTTTCGACTGGTAGTCAAATCGGAAGCAATGAGGATCCTCGGAATGGTGCTCAAAAAGGTTACGAAAACGGTCATGCTTCTCTGAATTCAGCTCCTGTGTCTCGAAGATAA

Protein Analysis

390

Amino Acids

44.11

Weight (kDa)

5.93

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 43 - 162 4.6e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
F420_oxidored PF03807 75 - 164 1.7e-11 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 90 - 198 5.2e-07 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 261 - 334 6.2e-35 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 262, 572, 584
AciI CCGC 2 cut(s) 1032, 1052
AclWI GGATC 3 cut(s) 960, 1091, 1104
AcoI YGGCCR 2 cut(s) 253, 264
AcsI RAATTY 3 cut(s) 791, 976, 1147
AdeI CACNNNGTG 1 cut(s) 584
AfaI GTAC 2 cut(s) 409, 884
AfiI CCNNNNNNNGG 5 cut(s) 262, 394, 572, 584, 1106
AflII CTTAAG 1 cut(s) 956
AgsI TTSAA 1 cut(s) 844
AhdI GACNNNNNGTC 2 cut(s) 181, 1074
AjnI CCWGG 1 cut(s) 266
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
AloI GAACNNNNNNTCC 2 cut(s) 929, 961
AluBI AGCT 3 cut(s) 873, 925, 1154
AluI AGCT 3 cut(s) 873, 925, 1154
Alw21I GWGCWC 2 cut(s) 732, 1114
Alw26I GTCTC 5 cut(s) 189, 451, 827, 991, 1167
AlwI GGATC 3 cut(s) 960, 1091, 1104
AlwNI CAGNNNCTG 1 cut(s) 1157
AoxI GGCC 2 cut(s) 253, 264
ApeKI GCWGC 3 cut(s) 161, 743, 922
ApoI RAATTY 3 cut(s) 791, 976, 1147
ArsI GACNNNNNNTTYG 2 cut(s) 929, 961
AseI ATTAAT 1 cut(s) 816
Asp700I GAANNNNTTC 1 cut(s) 396
AspS9I GGNCC 3 cut(s) 117, 270, 575
AsuII TTCGAA 1 cut(s) 466
AvaII GGWCC 3 cut(s) 117, 270, 575
BalI TGGCCA 1 cut(s) 266
BamHI GGATCC 1 cut(s) 1096
BauI CACGAG 1 cut(s) 595
BbsI GAAGAC 1 cut(s) 599
Bbv12I GWGCWC 2 cut(s) 732, 1114
BbvI GCAGC 3 cut(s) 148, 730, 934
BccI CCATC 3 cut(s) 67, 129, 161
BceAI ACGGC 2 cut(s) 268, 876
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 1 cut(s) 268
BcoDI GTCTC 5 cut(s) 189, 451, 827, 991, 1167
BfmI CTRYAG 1 cut(s) 744
BfrI CTTAAG 1 cut(s) 956
BisI GCNGC 4 cut(s) 162, 744, 923, 1032
BlpI GCTNAGC 1 cut(s) 726
BlsI GCNGC 4 cut(s) 163, 745, 924, 1033
Bme1390I CCNGG 1 cut(s) 268
Bme18I GGWCC 3 cut(s) 117, 270, 575
BmeRI GACNNNNNGTC 2 cut(s) 181, 1074
BmgT120I GGNCC 3 cut(s) 117, 270, 575
BmiI GGNNCC 3 cut(s) 118, 119, 1098
BmrFI CCNGG 1 cut(s) 268
BmsI GCATC 2 cut(s) 730, 1033
BpiI GAAGAC 1 cut(s) 599
BpmI CTGGAG 1 cut(s) 947
Bpu1102I GCTNAGC 1 cut(s) 726
Bpu14I TTCGAA 1 cut(s) 466
BsaJI CCNNGG 1 cut(s) 1100
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 5 cut(s) 262, 394, 572, 584, 1106
Bse1I ACTGG 3 cut(s) 256, 423, 1075
Bse3DI GCAATG 2 cut(s) 921, 1096
BseBI CCWGG 1 cut(s) 268
BseDI CCNNGG 1 cut(s) 1100
BseGI GGATG 2 cut(s) 745, 1048
BseLI CCNNNNNNNGG 5 cut(s) 262, 394, 572, 584, 1106
BseMI GCAATG 2 cut(s) 921, 1096
BseMII CTCAG 2 cut(s) 531, 717
BseNI ACTGG 3 cut(s) 256, 423, 1075
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 3 cut(s) 148, 730, 934
BseYI CCCAGC 1 cut(s) 863
BsgI GTGCAG 1 cut(s) 147
BshFI GGCC 2 cut(s) 255, 266
BsiHKAI GWGCWC 2 cut(s) 732, 1114
BslFI GGGAC 1 cut(s) 130
BslI CCNNNNNNNGG 5 cut(s) 262, 394, 572, 584, 1106
BsmAI GTCTC 5 cut(s) 189, 451, 827, 991, 1167
BsmFI GGGAC 1 cut(s) 130
BsmI GAATGC 1 cut(s) 916
BsnI GGCC 2 cut(s) 255, 266
Bsp119I TTCGAA 1 cut(s) 466
Bsp1286I GDGCHC 2 cut(s) 732, 1114
Bsp143I GATC 2 cut(s) 952, 1096
Bsp1720I GCTNAGC 1 cut(s) 726
BspACI CCGC 2 cut(s) 1032, 1052
BspANI GGCC 2 cut(s) 255, 266
BspCNI CTCAG 2 cut(s) 532, 718
BspLI GGNNCC 3 cut(s) 118, 119, 1098
BspMAI CTGCAG 1 cut(s) 748
BspPI GGATC 3 cut(s) 960, 1091, 1104
BspT104I TTCGAA 1 cut(s) 466
BspTI CTTAAG 1 cut(s) 956
BsrDI GCAATG 2 cut(s) 921, 1096
BsrI ACTGG 3 cut(s) 256, 423, 1075
BssECI CCNNGG 1 cut(s) 1100
BssMI GATC 2 cut(s) 952, 1096
BssSI CACGAG 1 cut(s) 595
Bst2BI CACGAG 1 cut(s) 595
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 3 cut(s) 295, 1002, 1133
Bst6I CTCTTC 3 cut(s) 19, 90, 1032
BstAFI CTTAAG 1 cut(s) 956
BstAPI GCANNNNNTGC 1 cut(s) 1060
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 2 cut(s) 540, 726
BstF5I GGATG 2 cut(s) 745, 1048
BstKTI GATC 2 cut(s) 955, 1099
BstMAI GTCTC 5 cut(s) 189, 451, 827, 991, 1167
BstMBI GATC 2 cut(s) 952, 1096
BstMWI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 931, 1060
BstNI CCWGG 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 266
BstSFI CTRYAG 1 cut(s) 744
BstV1I GCAGC 3 cut(s) 148, 730, 934
BstV2I GAAGAC 1 cut(s) 599
BstX2I RGATCY 2 cut(s) 952, 1096
BstYI RGATCY 2 cut(s) 952, 1096
BsuRI GGCC 2 cut(s) 255, 266
BtsCI GGATG 2 cut(s) 745, 1048
BtsIMutI CAGTG 2 cut(s) 291, 430
Cac8I GCNNGC 1 cut(s) 537
CaiI CAGNNNCTG 1 cut(s) 1157
Cfr13I GGNCC 3 cut(s) 117, 270, 575
CseI GACGC 1 cut(s) 167
Csp6I GTAC 2 cut(s) 408, 883
CviAII CATG 3 cut(s) 940, 983, 1136
CviQI GTAC 2 cut(s) 408, 883
DdeI CTNAG 2 cut(s) 540, 726
DpnI GATC 2 cut(s) 954, 1098
DpnII GATC 2 cut(s) 952, 1096
DraIII CACNNNGTG 1 cut(s) 584
DriI GACNNNNNGTC 2 cut(s) 181, 1074
EaeI YGGCCR 2 cut(s) 253, 264
Eam1104I CTCTTC 3 cut(s) 19, 90, 1032
Eam1105I GACNNNNNGTC 2 cut(s) 181, 1074
EarI CTCTTC 3 cut(s) 19, 90, 1032
Eco47I GGWCC 3 cut(s) 117, 270, 575
EcoO109I RGGNCCY 2 cut(s) 117, 270
EcoRI GAATTC 1 cut(s) 1147
EcoRII CCWGG 1 cut(s) 266
FaeI CATG 3 cut(s) 943, 986, 1139
FaqI GGGAC 1 cut(s) 130
FatI CATG 3 cut(s) 939, 982, 1135
Fnu4HI GCNGC 4 cut(s) 162, 744, 923, 1032
FokI GGATG 2 cut(s) 752, 1055
Fsp4HI GCNGC 4 cut(s) 162, 744, 923, 1032
GluI GCNGC 4 cut(s) 162, 744, 923, 1032
GsaI CCCAGC 1 cut(s) 867
GsuI CTGGAG 1 cut(s) 947
HaeIII GGCC 2 cut(s) 255, 266
HgaI GACGC 1 cut(s) 167
Hin1II CATG 3 cut(s) 943, 986, 1139
HinfI GANTC 3 cut(s) 182, 593, 803
Hpy166II GTNNAC 1 cut(s) 578
Hpy188I TCNGA 6 cut(s) 316, 456, 797, 1085, 1104, 1146
Hpy188III TCNNGA 1 cut(s) 1164
Hpy8I GTNNAC 1 cut(s) 578
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 4 cut(s) 42, 283, 454, 689
HpyCH4III ACNGT 3 cut(s) 295, 1002, 1133
HpyCH4V TGCA 6 cut(s) 164, 558, 746, 914, 982, 1046
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 931, 1060
HpyF3I CTNAG 2 cut(s) 540, 726
Hsp92II CATG 3 cut(s) 943, 986, 1139
KflI GGGWCCC 1 cut(s) 117
Kzo9I GATC 2 cut(s) 952, 1096
LmnI GCTCC 2 cut(s) 919, 1159
Lsp1109I GCAGC 3 cut(s) 148, 730, 934
LweI GCATC 2 cut(s) 730, 1033
MaeIII GTNAC 4 cut(s) 287, 750, 760, 1121
MalI GATC 2 cut(s) 954, 1098
MboI GATC 2 cut(s) 952, 1096
MboII GAAGA 7 cut(s) 6, 54, 77, 116, 455, 599, 1049
MflI RGATCY 2 cut(s) 952, 1096
MhlI GDGCHC 2 cut(s) 732, 1114
MlsI TGGCCA 1 cut(s) 266
MluCI AATT 6 cut(s) 225, 526, 755, 791, 976, 1147
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 3 cut(s) 191, 587, 812
MmeI TCCRAC 3 cut(s) 157, 304, 765
Mox20I TGGCCA 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 396
MscI TGGCCA 1 cut(s) 266
MseI TTAA 2 cut(s) 816, 957
Msp20I TGGCCA 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 925
MspCI CTTAAG 1 cut(s) 956
MspR9I CCNGG 1 cut(s) 268
Mva1269I GAATGC 1 cut(s) 916
MvaI CCWGG 1 cut(s) 268
MwoI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 931, 1060
NdeII GATC 2 cut(s) 952, 1096
NlaIII CATG 3 cut(s) 943, 986, 1139
NlaIV GGNNCC 3 cut(s) 118, 119, 1098
NmuCI GTSAC 3 cut(s) 287, 750, 760
NspV TTCGAA 1 cut(s) 466
PctI GAATGC 1 cut(s) 916
PdmI GAANNNNTTC 1 cut(s) 396
PflMI CCANNNNNTGG 3 cut(s) 262, 572, 584
PkrI GCNGC 4 cut(s) 163, 745, 924, 1033
PleI GAGTC 3 cut(s) 190, 587, 811
PpsI GAGTC 3 cut(s) 190, 587, 811
PpuMI RGGWCCY 2 cut(s) 117, 270
PshBI ATTAAT 1 cut(s) 816
Psp5II RGGWCCY 2 cut(s) 117, 270
Psp6I CCWGG 1 cut(s) 266
PspFI CCCAGC 1 cut(s) 863
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 3 cut(s) 118, 119, 1098
PspPI GGNCC 3 cut(s) 117, 270, 575
PspPPI RGGWCCY 2 cut(s) 117, 270
PstI CTGCAG 1 cut(s) 748
PstNI CAGNNNCTG 1 cut(s) 1157
PsuI RGATCY 2 cut(s) 952, 1096
PvuII CAGCTG 1 cut(s) 925
RsaI GTAC 2 cut(s) 409, 884
RsaNI GTAC 2 cut(s) 408, 883
SaqAI TTAA 2 cut(s) 816, 957
SatI GCNGC 4 cut(s) 162, 744, 923, 1032
Sau3AI GATC 2 cut(s) 952, 1096
Sau96I GGNCC 3 cut(s) 117, 270, 575
SchI GAGTC 3 cut(s) 191, 587, 812
ScrFI CCNGG 1 cut(s) 268
SduI GDGCHC 2 cut(s) 732, 1114
SfaNI GCATC 2 cut(s) 730, 1033
SfcI CTRYAG 1 cut(s) 744
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 3 cut(s) 117, 270, 575
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
Sse9I AATT 6 cut(s) 225, 526, 755, 791, 976, 1147
SsiI CCGC 2 cut(s) 1032, 1052
StyD4I CCNGG 1 cut(s) 266
TaaI ACNGT 3 cut(s) 295, 1002, 1133
TaqI TCGA 7 cut(s) 156, 413, 466, 948, 1026, 1067, 1165
TasI AATT 6 cut(s) 225, 526, 755, 791, 976, 1147
TatI WGTACW 2 cut(s) 407, 882
TauI GCSGC 1 cut(s) 1034
Tru1I TTAA 2 cut(s) 816, 957
Tru9I TTAA 2 cut(s) 816, 957
TscAI CASTG 2 cut(s) 298, 430
TseFI GTSAC 3 cut(s) 287, 750, 760
TseI GCWGC 3 cut(s) 161, 743, 922
Tsp45I GTSAC 3 cut(s) 287, 750, 760
TspDTI ATGAA 2 cut(s) 116, 1032
TspRI CASTG 2 cut(s) 298, 430
Van91I CCANNNNNTGG 3 cut(s) 262, 572, 584
Vha464I CTTAAG 1 cut(s) 956
VpaK11BI GGWCC 3 cut(s) 117, 270, 575
VspI ATTAAT 1 cut(s) 816
XapI RAATTY 3 cut(s) 791, 976, 1147
XmnI GAANNNNTTC 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.