Rw5G035370

Arogenate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012529
Physical Location & Seq
Reverse (-)
61162179 .. 61164694
2516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035370.1

Sequence Viewer

Length: 1095 bp
ATGCTCTCATTCTCTTCTCCTCAAGCCCAACCTTCACTCCTCCCTCCAAATCTCTACCCATCTTCTCACTCATTCTCTCTCTCTCTTCTTCCACCTCTAAAACCCAACAAAAACCAGAACCATCTTCATCTTCAACGCCTAACAATCCAAGCTATCGATGCTGCACTGTCCTTTGACTACGAGTCCCAACTCAAAACCCATAACCTAGAATCCAACACTCTCAAAATTGGCATCATTGGCTTCGGCAACTACGGCCAGTTTTTGGCCAGGTGCTTCGTCCAACAAGGTCACACTGTCCTCGCCCAATCTCGCTCCGACTACTCTAAAGTCGCGCAAGACTCTGGCGTTTCGTTCTTCACTGACCCCAATGACCTCTGTGAGCAACACCCAGAAGTGGTTCTGTTATGCACTTCGATTATGTCCACCGAAAAGGTCATCAAAACCTTCCCATTTCAAAGACTTCGTCGAAATACCTTATTTGTTGATGTTCTTTCAGTTAAGGAATTCCCAAGAGACTTGTTACTGACTTACTTGCCTGCTGAGTTTGACCTTCTTTGCACACACCCAATGTTTGGTCCACAGAGTGGTAAGAACTCGTGGGCTGGTTTTCCTTTTGTGTATGACAAGGTTAGAATTGGGGATAGTGGTTTTAGGCTCAATAGGTGTGAGAAGTTTTTGGACATTTTCGCTAGGGAAGGGTGTCGAATGGTGGAAATGTCGTGTGCTGAGCACGATAGGCATGCCGCAGGGTCACAATTTGTGACACATACAATTGGGAGAATGTTGGAGAAATTCGGATTGGAGTCTTCGCCGATTAATACTAAGGGGTATGAGACTTTATTGAACTTGGTGGAGAATACGGCTCAGGATAGCTTTGAGTTGTACTATGGTTTGTTTATATATAACAAGAATGCAATGGAGCAGTTGGAGAGGCTGGAAATCGCATTCGAGGATCTTAAGAAAGAGTTACGAAATCGGTCAGAACCGCAGAAGTTGCTTTCGATTGGTAGCCAAATCGGAAGCAATGAGGATCCTCAGAATGATACTCAAAAGGGTTACGAGAACACCCAAATGCTTCATTTCCTGAAACATTAG

Protein Analysis

364

Amino Acids

41.46

Weight (kDa)

6.19

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 53 - 162 4.7e-08 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
F420_oxidored PF03807 75 - 164 7.2e-10 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 95 - 197 3.7e-08 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 261 - 328 1.1e-31 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 262, 572, 584
AccII CGCG 1 cut(s) 332
AciI CCGC 2 cut(s) 744, 986
AclWI GGATC 3 cut(s) 960, 1025, 1038
AcoI YGGCCR 2 cut(s) 253, 264
AcsI RAATTY 2 cut(s) 503, 791
AdeI CACNNNGTG 1 cut(s) 584
AfaI GTAC 1 cut(s) 884
AfiI CCNNNNNNNGG 4 cut(s) 262, 394, 572, 584
AflII CTTAAG 1 cut(s) 956
AgsI TTSAA 3 cut(s) 134, 455, 844
AhdI GACNNNNNGTC 1 cut(s) 181
AjnI CCWGG 1 cut(s) 266
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
AluBI AGCT 2 cut(s) 152, 873
AluI AGCT 2 cut(s) 152, 873
Alw21I GWGCWC 1 cut(s) 732
Alw26I GTCTC 2 cut(s) 507, 827
AlwI GGATC 3 cut(s) 960, 1025, 1038
AoxI GGCC 2 cut(s) 253, 264
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 2 cut(s) 503, 791
ArsI GACNNNNNNTTYG 2 cut(s) 448, 480
AseI ATTAAT 1 cut(s) 816
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 1 cut(s) 334
AspS9I GGNCC 1 cut(s) 575
AvaII GGWCC 1 cut(s) 575
BalI TGGCCA 1 cut(s) 266
BamHI GGATCC 1 cut(s) 1030
BauI CACGAG 1 cut(s) 595
BbsI GAAGAC 1 cut(s) 798
Bbv12I GWGCWC 1 cut(s) 732
BbvI GCAGC 1 cut(s) 148
BccI CCATC 2 cut(s) 67, 129
BceAI ACGGC 2 cut(s) 268, 876
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 1 cut(s) 268
BcoDI GTCTC 2 cut(s) 507, 827
BfaI CTAG 2 cut(s) 206, 690
BfrI CTTAAG 1 cut(s) 956
BisI GCNGC 2 cut(s) 162, 744
BlpI GCTNAGC 1 cut(s) 726
BlsI GCNGC 2 cut(s) 163, 745
Bme1390I CCNGG 1 cut(s) 268
Bme18I GGWCC 1 cut(s) 575
BmeRI GACNNNNNGTC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 1 cut(s) 1032
BmrFI CCNGG 1 cut(s) 268
BmsI GCATC 2 cut(s) 148, 240
BpiI GAAGAC 1 cut(s) 798
Bpu10I CCTNAGC 1 cut(s) 864
Bpu1102I GCTNAGC 1 cut(s) 726
BpuEI CTTGAG 1 cut(s) 6
Bsa29I ATCGAT 1 cut(s) 156
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 394, 572, 584
Bse1I ACTGG 1 cut(s) 256
Bse3DI GCAATG 2 cut(s) 921, 1030
BseBI CCWGG 1 cut(s) 268
BseCI ATCGAT 1 cut(s) 156
BseLI CCNNNNNNNGG 4 cut(s) 262, 394, 572, 584
BseMI GCAATG 2 cut(s) 921, 1030
BseMII CTCAG 4 cut(s) 531, 717, 878, 1049
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 2 cut(s) 9, 29
BseXI GCAGC 1 cut(s) 148
BsgI GTGCAG 1 cut(s) 147
Bsh1236I CGCG 1 cut(s) 332
BshFI GGCC 2 cut(s) 255, 266
BshVI ATCGAT 1 cut(s) 156
BsiHKAI GWGCWC 1 cut(s) 732
BslFI GGGAC 1 cut(s) 169
BslI CCNNNNNNNGG 4 cut(s) 262, 394, 572, 584
BsmAI GTCTC 2 cut(s) 507, 827
BsmFI GGGAC 1 cut(s) 169
BsmI GAATGC 2 cut(s) 916, 944
BsnI GGCC 2 cut(s) 255, 266
Bsp1286I GDGCHC 1 cut(s) 732
Bsp143I GATC 2 cut(s) 952, 1030
Bsp1720I GCTNAGC 1 cut(s) 726
BspACI CCGC 2 cut(s) 744, 986
BspANI GGCC 2 cut(s) 255, 266
BspCNI CTCAG 4 cut(s) 532, 718, 877, 1048
BspDI ATCGAT 1 cut(s) 156
BspFNI CGCG 1 cut(s) 332
BspLI GGNNCC 1 cut(s) 1032
BspPI GGATC 3 cut(s) 960, 1025, 1038
BspTI CTTAAG 1 cut(s) 956
BsrDI GCAATG 2 cut(s) 921, 1030
BsrI ACTGG 1 cut(s) 256
BssMI GATC 2 cut(s) 952, 1030
BssSI CACGAG 1 cut(s) 595
Bst2BI CACGAG 1 cut(s) 595
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 2 cut(s) 168, 295
Bst6I CTCTTC 2 cut(s) 19, 90
BstAFI CTTAAG 1 cut(s) 956
BstAPI GCANNNNNTGC 1 cut(s) 994
BstC8I GCNNGC 2 cut(s) 537, 741
BstDEI CTNAG 5 cut(s) 540, 726, 822, 864, 1035
BstFNI CGCG 1 cut(s) 332
BstHHI GCGC 1 cut(s) 334
BstKTI GATC 2 cut(s) 955, 1033
BstMAI GTCTC 2 cut(s) 507, 827
BstMBI GATC 2 cut(s) 952, 1030
BstMWI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 736, 994
BstNI CCWGG 1 cut(s) 268
BstNSI RCATGY 1 cut(s) 743
BstSCI CCNGG 1 cut(s) 266
BstUI CGCG 1 cut(s) 332
BstV1I GCAGC 1 cut(s) 148
BstV2I GAAGAC 1 cut(s) 798
BstX2I RGATCY 2 cut(s) 952, 1030
BstYI RGATCY 2 cut(s) 952, 1030
Bsu15I ATCGAT 1 cut(s) 156
BsuRI GGCC 2 cut(s) 255, 266
BsuTUI ATCGAT 1 cut(s) 156
BtsIMutI CAGTG 3 cut(s) 164, 291, 357
Cac8I GCNNGC 2 cut(s) 537, 741
CfoI GCGC 1 cut(s) 334
Cfr13I GGNCC 1 cut(s) 575
ClaI ATCGAT 1 cut(s) 156
Csp6I GTAC 1 cut(s) 883
CviAII CATG 1 cut(s) 740
CviQI GTAC 1 cut(s) 883
DdeI CTNAG 5 cut(s) 540, 726, 822, 864, 1035
DpnI GATC 2 cut(s) 954, 1032
DpnII GATC 2 cut(s) 952, 1030
DraIII CACNNNGTG 1 cut(s) 584
DriI GACNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 2 cut(s) 253, 264
Eam1104I CTCTTC 2 cut(s) 19, 90
Eam1105I GACNNNNNGTC 1 cut(s) 181
EarI CTCTTC 2 cut(s) 19, 90
Eco47I GGWCC 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 266
FaeI CATG 1 cut(s) 743
FaqI GGGAC 1 cut(s) 169
FatI CATG 1 cut(s) 739
Fnu4HI GCNGC 2 cut(s) 162, 744
Fsp4HI GCNGC 2 cut(s) 162, 744
FspBI CTAG 2 cut(s) 206, 690
GlaI GCGC 1 cut(s) 333
GluI GCNGC 2 cut(s) 162, 744
HaeIII GGCC 2 cut(s) 255, 266
HhaI GCGC 1 cut(s) 334
Hin1II CATG 1 cut(s) 743
Hin6I GCGC 1 cut(s) 332
HinP1I GCGC 1 cut(s) 332
HinfI GANTC 4 cut(s) 182, 209, 338, 803
Hpy166II GTNNAC 2 cut(s) 423, 578
Hpy188I TCNGA 5 cut(s) 316, 797, 982, 1019, 1038
Hpy188III TCNNGA 2 cut(s) 866, 1084
Hpy8I GTNNAC 2 cut(s) 423, 578
Hpy99I CGWCG 1 cut(s) 468
HpyAV CCTTC 4 cut(s) 42, 454, 560, 689
HpyCH4III ACNGT 2 cut(s) 168, 295
HpyCH4V TGCA 4 cut(s) 164, 408, 558, 914
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 736, 994
HpyF3I CTNAG 5 cut(s) 540, 726, 822, 864, 1035
Hsp92II CATG 1 cut(s) 743
HspAI GCGC 1 cut(s) 332
Kzo9I GATC 2 cut(s) 952, 1030
LmnI GCTCC 2 cut(s) 317, 919
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 2 cut(s) 148, 240
MaeI CTAG 2 cut(s) 206, 690
MaeIII GTNAC 6 cut(s) 287, 519, 750, 760, 966, 1055
MalI GATC 2 cut(s) 954, 1032
MboI GATC 2 cut(s) 952, 1030
MboII GAAGA 8 cut(s) 6, 54, 77, 80, 116, 122, 346, 798
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 2 cut(s) 952, 1030
MhlI GDGCHC 1 cut(s) 732
MlsI TGGCCA 1 cut(s) 266
MluCI AATT 6 cut(s) 225, 503, 633, 755, 771, 791
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 3 cut(s) 191, 332, 812
MmeI TCCRAC 5 cut(s) 237, 304, 339, 765, 906
Mox20I TGGCCA 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 396
MscI TGGCCA 1 cut(s) 266
MseI TTAA 3 cut(s) 498, 816, 957
MslI CAYNNNNRTG 1 cut(s) 1070
Msp20I TGGCCA 1 cut(s) 266
MspCI CTTAAG 1 cut(s) 956
MspR9I CCNGG 1 cut(s) 268
MunI CAATTG 1 cut(s) 771
Mva1269I GAATGC 2 cut(s) 916, 944
MvaI CCWGG 1 cut(s) 268
MvnI CGCG 1 cut(s) 332
MwoI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 736, 994
NdeII GATC 2 cut(s) 952, 1030
NlaIII CATG 1 cut(s) 743
NlaIV GGNNCC 1 cut(s) 1032
NmuCI GTSAC 3 cut(s) 287, 750, 760
NspI RCATGY 1 cut(s) 743
PaeI GCATGC 1 cut(s) 743
PctI GAATGC 2 cut(s) 916, 944
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 1 cut(s) 209
PflFI GACNNNGTC 1 cut(s) 462
PflMI CCANNNNNTGG 3 cut(s) 262, 572, 584
PkrI GCNGC 2 cut(s) 163, 745
PleI GAGTC 3 cut(s) 190, 332, 811
PpsI GAGTC 3 cut(s) 190, 332, 811
PshBI ATTAAT 1 cut(s) 816
Psp6I CCWGG 1 cut(s) 266
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 1 cut(s) 1032
PspPI GGNCC 1 cut(s) 575
PsuI RGATCY 2 cut(s) 952, 1030
PsyI GACNNNGTC 1 cut(s) 462
RsaI GTAC 1 cut(s) 884
RsaNI GTAC 1 cut(s) 883
RseI CAYNNNNRTG 1 cut(s) 1070
SaqAI TTAA 3 cut(s) 498, 816, 957
SatI GCNGC 2 cut(s) 162, 744
Sau3AI GATC 2 cut(s) 952, 1030
Sau96I GGNCC 1 cut(s) 575
SchI GAGTC 3 cut(s) 191, 332, 812
ScrFI CCNGG 1 cut(s) 268
SduI GDGCHC 1 cut(s) 732
SfaNI GCATC 2 cut(s) 148, 240
SinI GGWCC 1 cut(s) 575
SmiMI CAYNNNNRTG 1 cut(s) 1070
SmlI CTYRAG 2 cut(s) 21, 956
SmoI CTYRAG 2 cut(s) 21, 956
SphI GCATGC 1 cut(s) 743
Sse9I AATT 6 cut(s) 225, 503, 633, 755, 771, 791
SsiI CCGC 2 cut(s) 744, 986
SspMI CTAG 2 cut(s) 206, 690
StyD4I CCNGG 1 cut(s) 266
TaaI ACNGT 2 cut(s) 168, 295
TaqI TCGA 6 cut(s) 156, 413, 466, 703, 948, 1001
TaqII GACCGA 1 cut(s) 966
TasI AATT 6 cut(s) 225, 503, 633, 755, 771, 791
TatI WGTACW 1 cut(s) 882
TauI GCSGC 1 cut(s) 746
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 3 cut(s) 498, 816, 957
Tru9I TTAA 3 cut(s) 498, 816, 957
TscAI CASTG 3 cut(s) 171, 298, 364
TseFI GTSAC 3 cut(s) 287, 750, 760
TseI GCWGC 1 cut(s) 161
Tsp45I GTSAC 3 cut(s) 287, 750, 760
TspDTI ATGAA 2 cut(s) 116, 1067
TspRI CASTG 3 cut(s) 171, 298, 364
Tth111I GACNNNGTC 1 cut(s) 462
Van91I CCANNNNNTGG 3 cut(s) 262, 572, 584
Vha464I CTTAAG 1 cut(s) 956
VpaK11BI GGWCC 1 cut(s) 575
VspI ATTAAT 1 cut(s) 816
XapI RAATTY 2 cut(s) 503, 791
XceI RCATGY 1 cut(s) 743
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 2 cut(s) 206, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.