Rh2BG376200

Arogenate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012529
Physical Location & Seq
Reverse (-)
53134447 .. 53135887
1441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG376200.1

Sequence Viewer

Length: 1155 bp
ATGCTCTCATTCTCTTTCACTCAAACCAAACCTTCACTCCTCCCTCCAAATCTCTACCCATCTTCTCACTCATTCTCTCACTCTCTTCTCCCACCTCTAAAACCCGACAAAAACCGGACCCATCTTCATCTCCAAAGCCTAACAATCCAAGCCATCGATGCTGCACAGCCCTTTGACTACGAGTCTCAACTCAAAACCCACTACCAAAAATCCAATGCTCTCAAAATTGGCATTATTGGCTTCGGCAACTACGGCCAGTTCTTGGCCAGGTCCTTCGTCCAACAAGGTCACACTGTCCTCGCCCATTCTCGCTCTGACTACTCCAAACTCGCCCAAGAAGTCGGCGTTTCGTTCTTTACCGACCCCAATGACCTCTGTGAGCAACACCCAGAAGTGGTTCTCTTATGTACTTCGATTATGTCCAGTGAAAAGGTAATCAAAACCTTCCCATTTCAGAGACTTCTTCGAAATACCTTATTTGTTGATGTTCTTTCAGTCAAGGAGTTCCCAAGAGACTTGTTCCTCAATTACTTGCCTGCTGAGTTTGACATTCTTTGCACACACCCAATGTTTGGTCCACAGAGTGGTAAGGACTCGTGGGTTGGTCTTCCTTTTGTGTATGACAAGGTTAGGATTGGGGATTGTGGTTTTAGGCTCAATAGGTGTGAGAAGTTTTTGGACATTTTCGCAAGGGAAGGGTGTAGAATGGTGGAAATGTCGTGTGCCGAGCACGATAGGGATGCTGCAGGGTCACAATTTGTCACACACACAATGGGGAGAGTGTTGGAGAAATTCAGATTGGAGTCCTCGCCGATTAATACTAAAGGGTATGAGACTTTGTTGAACTTGGTGGAGAATACGGCTGGGGATAGCTTTGAGTTGTACTATGGGTTGTTTATGTATAACAAGAATGCAATGGAGCAGCTGGAGAGGCTGGACATGGCATTCGAGGATCTTAAGAAAGAGTTGTTTGGGAATTTGCATGAGGTTTGTAGGAGACAGTTGTTTGGGAGTGCTGAACGGGTCGAAGCCTTAAGAGAAGAAGAGGATGCAGAACCGCAGGAGTTGCTTTCGACTGGTAGTCAAATCGGAAGCAATGAGGATCGTCGGAATGGTGCGCTAAAAAAGGTTACGAAAATGGTCATGCTTCTCTGA

Protein Analysis

384

Amino Acids

43.78

Weight (kDa)

5.98

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 60 - 162 1.1e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
F420_oxidored PF03807 75 - 164 1.7e-11 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 90 - 198 5e-07 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 261 - 334 6.1e-35 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 262, 572, 584
AciI CCGC 1 cut(s) 1058
AclWI GGATC 2 cut(s) 960, 1110
AcoI YGGCCR 2 cut(s) 253, 264
AcsI RAATTY 2 cut(s) 791, 976
AdeI CACNNNGTG 1 cut(s) 584
AfaI GTAC 2 cut(s) 409, 884
AfiI CCNNNNNNNGG 4 cut(s) 262, 394, 572, 584
AflII CTTAAG 2 cut(s) 956, 1033
AgsI TTSAA 1 cut(s) 844
AhdI GACNNNNNGTC 2 cut(s) 181, 1080
AjnI CCWGG 1 cut(s) 266
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
AluBI AGCT 2 cut(s) 873, 925
AluI AGCT 2 cut(s) 873, 925
Alw21I GWGCWC 1 cut(s) 732
Alw26I GTCTC 5 cut(s) 189, 451, 507, 827, 991
AlwI GGATC 2 cut(s) 960, 1110
AoxI GGCC 2 cut(s) 253, 264
ApeKI GCWGC 3 cut(s) 161, 743, 922
ApoI RAATTY 2 cut(s) 791, 976
ArsI GACNNNNNNTTYG 2 cut(s) 929, 961
AseI ATTAAT 1 cut(s) 816
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 1 cut(s) 1120
AspS9I GGNCC 3 cut(s) 117, 270, 575
AsuII TTCGAA 1 cut(s) 466
AvaII GGWCC 3 cut(s) 117, 270, 575
BalI TGGCCA 1 cut(s) 266
BauI CACGAG 1 cut(s) 595
BbsI GAAGAC 1 cut(s) 599
Bbv12I GWGCWC 1 cut(s) 732
BbvI GCAGC 3 cut(s) 148, 730, 934
BccI CCATC 3 cut(s) 67, 129, 161
BceAI ACGGC 2 cut(s) 268, 876
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 1 cut(s) 268
BcoDI GTCTC 5 cut(s) 189, 451, 507, 827, 991
BfmI CTRYAG 1 cut(s) 744
BfrI CTTAAG 2 cut(s) 956, 1033
BisI GCNGC 3 cut(s) 162, 744, 923
BlsI GCNGC 3 cut(s) 163, 745, 924
Bme1390I CCNGG 1 cut(s) 268
Bme18I GGWCC 3 cut(s) 117, 270, 575
BmeRI GACNNNNNGTC 2 cut(s) 181, 1080
BmgT120I GGNCC 3 cut(s) 117, 270, 575
BmiI GGNNCC 1 cut(s) 119
BmrFI CCNGG 1 cut(s) 268
BmsI GCATC 3 cut(s) 148, 730, 1039
BpiI GAAGAC 1 cut(s) 599
BpmI CTGGAG 1 cut(s) 947
Bpu14I TTCGAA 1 cut(s) 466
Bsa29I ATCGAT 1 cut(s) 156
BsaWI WCCGGW 1 cut(s) 114
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 394, 572, 584
Bse1I ACTGG 3 cut(s) 256, 423, 1081
Bse3DI GCAATG 2 cut(s) 921, 1102
BseBI CCWGG 1 cut(s) 268
BseCI ATCGAT 1 cut(s) 156
BseGI GGATG 2 cut(s) 745, 1054
BseLI CCNNNNNNNGG 4 cut(s) 262, 394, 572, 584
BseMI GCAATG 2 cut(s) 921, 1102
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 3 cut(s) 256, 423, 1081
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 3 cut(s) 148, 730, 934
BseYI CCCAGC 1 cut(s) 863
BsgI GTGCAG 1 cut(s) 147
BshFI GGCC 2 cut(s) 255, 266
BshVI ATCGAT 1 cut(s) 156
BsiHKAI GWGCWC 1 cut(s) 732
BsiSI CCGG 1 cut(s) 115
BslI CCNNNNNNNGG 4 cut(s) 262, 394, 572, 584
BsmAI GTCTC 5 cut(s) 189, 451, 507, 827, 991
BsmI GAATGC 2 cut(s) 916, 944
BsnI GGCC 2 cut(s) 255, 266
Bsp119I TTCGAA 1 cut(s) 466
Bsp1286I GDGCHC 1 cut(s) 732
Bsp143I GATC 2 cut(s) 952, 1102
BspACI CCGC 1 cut(s) 1058
BspANI GGCC 2 cut(s) 255, 266
BspCNI CTCAG 1 cut(s) 532
BspDI ATCGAT 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 119
BspMAI CTGCAG 1 cut(s) 748
BspPI GGATC 2 cut(s) 960, 1110
BspT104I TTCGAA 1 cut(s) 466
BspTI CTTAAG 2 cut(s) 956, 1033
BsrDI GCAATG 2 cut(s) 921, 1102
BsrI ACTGG 3 cut(s) 256, 423, 1081
BssMI GATC 2 cut(s) 952, 1102
BssSI CACGAG 1 cut(s) 595
Bst2BI CACGAG 1 cut(s) 595
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 2 cut(s) 295, 1002
Bst6I CTCTTC 2 cut(s) 90, 1038
BstAFI CTTAAG 2 cut(s) 956, 1033
BstAPI GCANNNNNTGC 1 cut(s) 1066
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 1 cut(s) 540
BstF5I GGATG 2 cut(s) 745, 1054
BstHHI GCGC 1 cut(s) 1120
BstKTI GATC 2 cut(s) 955, 1105
BstMAI GTCTC 5 cut(s) 189, 451, 507, 827, 991
BstMBI GATC 2 cut(s) 952, 1102
BstMWI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 931, 1066
BstNI CCWGG 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 266
BstSFI CTRYAG 1 cut(s) 744
BstV1I GCAGC 3 cut(s) 148, 730, 934
BstV2I GAAGAC 1 cut(s) 599
BstX2I RGATCY 1 cut(s) 952
BstYI RGATCY 1 cut(s) 952
Bsu15I ATCGAT 1 cut(s) 156
BsuRI GGCC 2 cut(s) 255, 266
BsuTUI ATCGAT 1 cut(s) 156
BtsCI GGATG 2 cut(s) 745, 1054
BtsIMutI CAGTG 2 cut(s) 291, 430
Cac8I GCNNGC 1 cut(s) 537
CfoI GCGC 1 cut(s) 1120
Cfr13I GGNCC 3 cut(s) 117, 270, 575
ClaI ATCGAT 1 cut(s) 156
Csp6I GTAC 2 cut(s) 408, 883
CviAII CATG 3 cut(s) 940, 983, 1144
CviQI GTAC 2 cut(s) 408, 883
DdeI CTNAG 1 cut(s) 540
DpnI GATC 2 cut(s) 954, 1104
DpnII GATC 2 cut(s) 952, 1102
DraIII CACNNNGTG 1 cut(s) 584
DriI GACNNNNNGTC 2 cut(s) 181, 1080
EaeI YGGCCR 2 cut(s) 253, 264
Eam1104I CTCTTC 2 cut(s) 90, 1038
Eam1105I GACNNNNNGTC 2 cut(s) 181, 1080
EarI CTCTTC 2 cut(s) 90, 1038
Eco47I GGWCC 3 cut(s) 117, 270, 575
EcoO109I RGGNCCY 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 266
FaeI CATG 3 cut(s) 943, 986, 1147
FatI CATG 3 cut(s) 939, 982, 1143
Fnu4HI GCNGC 3 cut(s) 162, 744, 923
FokI GGATG 2 cut(s) 752, 1061
Fsp4HI GCNGC 3 cut(s) 162, 744, 923
GlaI GCGC 1 cut(s) 1119
GluI GCNGC 3 cut(s) 162, 744, 923
GsaI CCCAGC 1 cut(s) 867
GsuI CTGGAG 1 cut(s) 947
HaeIII GGCC 2 cut(s) 255, 266
HapII CCGG 1 cut(s) 115
HhaI GCGC 1 cut(s) 1120
Hin1II CATG 3 cut(s) 943, 986, 1147
Hin6I GCGC 1 cut(s) 1118
HinP1I GCGC 1 cut(s) 1118
HinfI GANTC 3 cut(s) 182, 593, 803
HpaII CCGG 1 cut(s) 115
Hpy166II GTNNAC 1 cut(s) 578
Hpy188I TCNGA 6 cut(s) 316, 456, 797, 1091, 1110, 1154
Hpy8I GTNNAC 1 cut(s) 578
Hpy99I CGWCG 1 cut(s) 1110
HpyAV CCTTC 4 cut(s) 42, 283, 454, 689
HpyCH4III ACNGT 2 cut(s) 295, 1002
HpyCH4V TGCA 6 cut(s) 164, 558, 746, 914, 982, 1052
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 931, 1066
HpyF3I CTNAG 1 cut(s) 540
Hsp92II CATG 3 cut(s) 943, 986, 1147
HspAI GCGC 1 cut(s) 1118
Kzo9I GATC 2 cut(s) 952, 1102
LmnI GCTCC 1 cut(s) 919
Lsp1109I GCAGC 3 cut(s) 148, 730, 934
LweI GCATC 3 cut(s) 148, 730, 1039
MaeIII GTNAC 4 cut(s) 287, 750, 760, 1129
MalI GATC 2 cut(s) 954, 1104
MboI GATC 2 cut(s) 952, 1102
MboII GAAGA 7 cut(s) 54, 77, 116, 455, 599, 1052, 1055
MflI RGATCY 1 cut(s) 952
MhlI GDGCHC 1 cut(s) 732
MlsI TGGCCA 1 cut(s) 266
MluCI AATT 5 cut(s) 225, 526, 755, 791, 976
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 3 cut(s) 191, 587, 812
MmeI TCCRAC 3 cut(s) 304, 765, 1088
Mox20I TGGCCA 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 396
MscI TGGCCA 1 cut(s) 266
MseI TTAA 3 cut(s) 816, 957, 1034
Msp20I TGGCCA 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 925
MspCI CTTAAG 2 cut(s) 956, 1033
MspI CCGG 1 cut(s) 115
MspR9I CCNGG 1 cut(s) 268
Mva1269I GAATGC 2 cut(s) 916, 944
MvaI CCWGG 1 cut(s) 268
MwoI GCNNNNNNNGC 5 cut(s) 158, 237, 252, 931, 1066
NdeII GATC 2 cut(s) 952, 1102
NlaIII CATG 3 cut(s) 943, 986, 1147
NlaIV GGNNCC 1 cut(s) 119
NmeAIII GCCGAG 1 cut(s) 751
NmuCI GTSAC 3 cut(s) 287, 750, 760
NspV TTCGAA 1 cut(s) 466
PctI GAATGC 2 cut(s) 916, 944
PdmI GAANNNNTTC 1 cut(s) 396
PflMI CCANNNNNTGG 3 cut(s) 262, 572, 584
PkrI GCNGC 3 cut(s) 163, 745, 924
PleI GAGTC 3 cut(s) 190, 587, 811
PpsI GAGTC 3 cut(s) 190, 587, 811
PpuMI RGGWCCY 1 cut(s) 270
PshBI ATTAAT 1 cut(s) 816
Psp5II RGGWCCY 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 266
PspFI CCCAGC 1 cut(s) 863
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 1 cut(s) 119
PspPI GGNCC 3 cut(s) 117, 270, 575
PspPPI RGGWCCY 1 cut(s) 270
PstI CTGCAG 1 cut(s) 748
PsuI RGATCY 1 cut(s) 952
PvuII CAGCTG 1 cut(s) 925
RsaI GTAC 2 cut(s) 409, 884
RsaNI GTAC 2 cut(s) 408, 883
SaqAI TTAA 3 cut(s) 816, 957, 1034
SatI GCNGC 3 cut(s) 162, 744, 923
Sau3AI GATC 2 cut(s) 952, 1102
Sau96I GGNCC 3 cut(s) 117, 270, 575
SchI GAGTC 3 cut(s) 191, 587, 812
ScrFI CCNGG 1 cut(s) 268
SduI GDGCHC 1 cut(s) 732
SfaNI GCATC 3 cut(s) 148, 730, 1039
SfcI CTRYAG 1 cut(s) 744
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 3 cut(s) 117, 270, 575
SmlI CTYRAG 2 cut(s) 956, 1033
SmoI CTYRAG 2 cut(s) 956, 1033
Sse9I AATT 5 cut(s) 225, 526, 755, 791, 976
SsiI CCGC 1 cut(s) 1058
StyD4I CCNGG 1 cut(s) 266
TaaI ACNGT 2 cut(s) 295, 1002
TaqI TCGA 6 cut(s) 156, 413, 466, 948, 1026, 1073
TasI AATT 5 cut(s) 225, 526, 755, 791, 976
TatI WGTACW 2 cut(s) 407, 882
Tru1I TTAA 3 cut(s) 816, 957, 1034
Tru9I TTAA 3 cut(s) 816, 957, 1034
TscAI CASTG 2 cut(s) 298, 430
TseFI GTSAC 3 cut(s) 287, 750, 760
TseI GCWGC 3 cut(s) 161, 743, 922
Tsp45I GTSAC 3 cut(s) 287, 750, 760
TspDTI ATGAA 1 cut(s) 116
TspRI CASTG 2 cut(s) 298, 430
Van91I CCANNNNNTGG 3 cut(s) 262, 572, 584
Vha464I CTTAAG 2 cut(s) 956, 1033
VpaK11BI GGWCC 3 cut(s) 117, 270, 575
VspI ATTAAT 1 cut(s) 816
XapI RAATTY 2 cut(s) 791, 976
XmnI GAANNNNTTC 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.