RLG00000019693

DNA polymerase epsilon subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009448
Physical Location & Seq
Reverse (-)
55529252 .. 55529892
641 bp
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UTR
Exon/CDS
Intron
RLM00000019693

Sequence Viewer

Length: 369 bp
ATGTCCTTCTCCGAGAGCGCCCGCATCTTCATCCACTACCTCTCCGCCACAGCCAACGACATCTGCAAGGGATCGAAGAGGCAGACGATCAACGACGACGATGTTTTGAAGGCGTTGGAGGACATTGAGTTTCCCGAGTTCATCCAGCCTCTGAAGGCCTCTCTTGAAGGGCTGAGGGAGATGAATGCTGGGAAGAAGGCAAGAACATCAAAGGCAAATGAAGCGAAAACAAAAGAGGCAAAAACAAAGGAAGTGAAAAAGAAGAGGAAATTGGAAGAGCTGCCGCCCAACGAAGAGGAAGAGGAAGAGGAAGAAATCCAAGATGAGGATGGGGGCGATGGCGCTAGCGAAAGCTCGAGCAAAGAATGA

Protein Analysis

123

Amino Acids

13.69

Weight (kDa)

4.83

Isoelectric Point (pI)

71.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 5 - 39 8.9e-06 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 22, 45, 284
AclWI GGATC 1 cut(s) 79
AcuI CTGAAG 1 cut(s) 173
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 2 cut(s) 109, 167
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
AluBI AGCT 2 cut(s) 280, 354
AluI AGCT 2 cut(s) 280, 354
AlwI GGATC 1 cut(s) 79
AlwNI CAGNNNCTG 1 cut(s) 151
Ama87I CYCGRG 2 cut(s) 134, 355
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 280
AspLEI GCGC 2 cut(s) 20, 344
AsuNHI GCTAGC 1 cut(s) 344
AvaI CYCGRG 2 cut(s) 134, 355
BbvCI CCTCAGC 1 cut(s) 173
BbvI GCAGC 1 cut(s) 267
BccI CCATC 2 cut(s) 323, 332
BfaI CTAG 1 cut(s) 345
BfoI RGCGCY 2 cut(s) 21, 345
BisI GCNGC 2 cut(s) 281, 284
BlsI GCNGC 2 cut(s) 282, 285
BmeT110I CYCGRG 2 cut(s) 134, 355
BmsI GCATC 1 cut(s) 33
BmtI GCTAGC 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 173
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 1 cut(s) 325
BseGI GGATG 3 cut(s) 30, 141, 334
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMII CTCAG 1 cut(s) 164
BseXI GCAGC 1 cut(s) 267
BseYI CCCAGC 1 cut(s) 188
BshFI GGCC 1 cut(s) 158
BsiHKCI CYCGRG 2 cut(s) 134, 355
BslI CCNNNNNNNGG 1 cut(s) 325
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 1 cut(s) 158
BsoBI CYCGRG 2 cut(s) 134, 355
Bsp143I GATC 2 cut(s) 71, 87
BspACI CCGC 3 cut(s) 22, 45, 284
BspANI GGCC 1 cut(s) 158
BspCNI CTCAG 1 cut(s) 165
BspOI GCTAGC 1 cut(s) 348
BspPI GGATC 1 cut(s) 79
BspQI GCTCTTC 1 cut(s) 270
BssMI GATC 2 cut(s) 71, 87
Bst6I CTCTTC 6 cut(s) 71, 257, 270, 288, 294, 300
BstC8I GCNNGC 2 cut(s) 22, 346
BstDEI CTNAG 1 cut(s) 173
BstF5I GGATG 3 cut(s) 30, 141, 334
BstH2I RGCGCY 2 cut(s) 21, 345
BstHHI GCGC 2 cut(s) 20, 344
BstKTI GATC 2 cut(s) 74, 90
BstMBI GATC 2 cut(s) 71, 87
BstMWI GCNNNNNNNGC 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 267
BsuRI GGCC 1 cut(s) 158
BtgZI GCGATG 1 cut(s) 351
BtsCI GGATG 3 cut(s) 30, 141, 334
Cac8I GCNNGC 2 cut(s) 22, 346
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 2 cut(s) 20, 344
CviJI RGCY 6 cut(s) 53, 148, 158, 172, 280, 354
CviKI_1 RGCY 6 cut(s) 53, 148, 158, 172, 280, 354
DdeI CTNAG 1 cut(s) 173
DpnI GATC 2 cut(s) 73, 89
DpnII GATC 2 cut(s) 71, 87
Eam1104I CTCTTC 6 cut(s) 71, 257, 270, 288, 294, 300
EarI CTCTTC 6 cut(s) 71, 257, 270, 288, 294, 300
EciI GGCGGA 1 cut(s) 34
Eco147I AGGCCT 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 173
Eco88I CYCGRG 2 cut(s) 134, 355
FauI CCCGC 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 281, 284
FokI GGATG 3 cut(s) 17, 128, 341
Fsp4HI GCNGC 2 cut(s) 281, 284
FspBI CTAG 1 cut(s) 345
GlaI GCGC 2 cut(s) 19, 343
GluI GCNGC 2 cut(s) 281, 284
GsaI CCCAGC 1 cut(s) 192
HaeII RGCGCY 2 cut(s) 21, 345
HaeIII GGCC 1 cut(s) 158
HhaI GCGC 2 cut(s) 20, 344
Hin6I GCGC 2 cut(s) 18, 342
HinP1I GCGC 2 cut(s) 18, 342
Hpy188I TCNGA 2 cut(s) 13, 153
Hpy188III TCNNGA 2 cut(s) 134, 164
Hpy99I CGWCG 2 cut(s) 98, 101
HpyAV CCTTC 5 cut(s) 16, 103, 148, 161, 190
HpyCH4V TGCA 1 cut(s) 66
HpyF10VI GCNNNNNNNGC 1 cut(s) 221
HpyF3I CTNAG 1 cut(s) 173
HspAI GCGC 2 cut(s) 18, 342
Kzo9I GATC 2 cut(s) 71, 87
LguI GCTCTTC 1 cut(s) 270
LpnPI CCDG 2 cut(s) 158, 174
Lsp1109I GCAGC 1 cut(s) 267
LweI GCATC 1 cut(s) 33
MaeI CTAG 1 cut(s) 345
MalI GATC 2 cut(s) 73, 89
MboI GATC 2 cut(s) 71, 87
MboII GAAGA 9 cut(s) 19, 88, 205, 274, 287, 305, 311, 317, 323
MluCI AATT 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 96
Mva1269I GAATGC 1 cut(s) 190
MwoI GCNNNNNNNGC 1 cut(s) 221
NdeII GATC 2 cut(s) 71, 87
NheI GCTAGC 1 cut(s) 344
PaeR7I CTCGAG 1 cut(s) 355
PceI AGGCCT 1 cut(s) 158
PciSI GCTCTTC 1 cut(s) 270
PctI GAATGC 1 cut(s) 190
PkrI GCNGC 2 cut(s) 282, 285
PspFI CCCAGC 1 cut(s) 188
PspXI VCTCGAGB 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 151
SapI GCTCTTC 1 cut(s) 270
SatI GCNGC 2 cut(s) 281, 284
Sau3AI GATC 2 cut(s) 71, 87
SetI ASST 3 cut(s) 42, 282, 356
SfaNI GCATC 1 cut(s) 33
Sfr274I CTCGAG 1 cut(s) 355
SlaI CTCGAG 1 cut(s) 355
SmlI CTYRAG 1 cut(s) 355
SmoI CTYRAG 1 cut(s) 355
Sse9I AATT 1 cut(s) 269
SseBI AGGCCT 1 cut(s) 158
SsiI CCGC 3 cut(s) 22, 45, 284
SspMI CTAG 1 cut(s) 345
StuI AGGCCT 1 cut(s) 158
TaqI TCGA 2 cut(s) 74, 356
TasI AATT 1 cut(s) 269
TauI GCSGC 1 cut(s) 286
TseI GCWGC 1 cut(s) 280
TspDTI ATGAA 4 cut(s) 19, 130, 197, 234
XcmI CCANNNNNNNNNTGG 1 cut(s) 326
XhoI CTCGAG 1 cut(s) 355
XspI CTAG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.