RLG00000020127

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008142
Physical Location & Seq
Reverse (-)
61376486 .. 61376902
417 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020127

Sequence Viewer

Length: 309 bp
ATGGCCACCGAAATGCTGCTATCATCTAGCAACCAAAACCAGATGATGAAATTCCCCAAATTTGGGTGCCGACAAGTTAAGGTTCACTCTTCTTCTTACAATCACTCGCTTAGTTCTGGGAATTCTTCTTCTTTTGTCCTCCTGGAATACCTCAATGCTCCTCCATTGTCACCAACAGCACTCCATCATGCAATGATCAGGATCCGCTTTGCCGAAACTATCATGAAATCTCATAAGATAAGGCTTGAGCAGATTATAAGCGATCCGGTAAAGGTGCATAAAGGATTGGCAGAACTAAAGAGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

11.54

Weight (kDa)

10.08

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
AccB1I GGYRCC 1 cut(s) 66
AciI CCGC 1 cut(s) 205
AclWI GGATC 3 cut(s) 196, 209, 257
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 50, 59, 121
AfiI CCNNNNNNNGG 2 cut(s) 62, 63
AjnI CCWGG 1 cut(s) 141
AlwI GGATC 3 cut(s) 196, 209, 257
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 16
ApoI RAATTY 3 cut(s) 50, 59, 121
AsuHPI GGTGA 1 cut(s) 162
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 201
BanI GGYRCC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 3
BccI CCATC 1 cut(s) 192
BciT130I CCWGG 1 cut(s) 143
BclI TGATCA 1 cut(s) 195
BfaI CTAG 1 cut(s) 27
BisI GCNGC 1 cut(s) 17
BlsI GCNGC 1 cut(s) 18
Bme1390I CCNGG 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 68, 203
BmrFI CCNGG 1 cut(s) 143
BpuEI CTTGAG 1 cut(s) 266
BsaBI GATNNNNATC 1 cut(s) 200
BsaWI WCCGGW 1 cut(s) 265
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 63
Bse3DI GCAATG 1 cut(s) 198
Bse8I GATNNNNATC 1 cut(s) 200
BseBI CCWGG 1 cut(s) 143
BseJI GATNNNNATC 1 cut(s) 200
BseLI CCNNNNNNNGG 2 cut(s) 62, 63
BseMI GCAATG 1 cut(s) 198
BseRI GAGGAG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 3
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 66
BsiSI CCGG 1 cut(s) 266
BslI CCNNNNNNNGG 2 cut(s) 62, 63
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 3 cut(s) 195, 201, 262
BspACI CCGC 1 cut(s) 205
BspANI GGCC 1 cut(s) 5
BspHI TCATGA 1 cut(s) 222
BspLI GGNNCC 2 cut(s) 68, 203
BspPI GGATC 3 cut(s) 196, 209, 257
BspT107I GGYRCC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 198
BssMI GATC 3 cut(s) 195, 201, 262
Bst2UI CCWGG 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 94
BstDEI CTNAG 1 cut(s) 110
BstKTI GATC 3 cut(s) 198, 204, 265
BstMBI GATC 3 cut(s) 195, 201, 262
BstNI CCWGG 1 cut(s) 143
BstSCI CCNGG 1 cut(s) 141
BstV1I GCAGC 1 cut(s) 3
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
BsuRI GGCC 1 cut(s) 5
CciI TCATGA 1 cut(s) 222
CviAII CATG 2 cut(s) 188, 223
CviJI RGCY 2 cut(s) 5, 244
CviKI_1 RGCY 2 cut(s) 5, 244
DdeI CTNAG 1 cut(s) 110
DpnI GATC 3 cut(s) 197, 203, 264
DpnII GATC 3 cut(s) 195, 201, 262
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
EcoRI GAATTC 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 141
FaeI CATG 2 cut(s) 191, 226
FaiI YATR 5 cut(s) 189, 224, 234, 257, 279
FatI CATG 2 cut(s) 187, 222
FbaI TGATCA 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 17
FspBI CTAG 1 cut(s) 27
GluI GCNGC 1 cut(s) 17
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 266
Hin1II CATG 2 cut(s) 191, 226
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 162
Hpy166II GTNNAC 1 cut(s) 85
Hpy188III TCNNGA 2 cut(s) 199, 223
Hpy8I GTNNAC 1 cut(s) 85
HpyCH4V TGCA 2 cut(s) 191, 277
HpyF3I CTNAG 1 cut(s) 110
Hsp92II CATG 2 cut(s) 191, 226
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 3 cut(s) 195, 201, 262
LmnI GCTCC 1 cut(s) 163
LpnPI CCDG 6 cut(s) 53, 102, 128, 155, 184, 279
Lsp1109I GCAGC 1 cut(s) 3
MaeI CTAG 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 168
MalI GATC 3 cut(s) 197, 203, 264
MboI GATC 3 cut(s) 195, 201, 262
MboII GAAGA 4 cut(s) 81, 84, 117, 120
MflI RGATCY 1 cut(s) 201
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 50, 59, 121
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 3 cut(s) 149, 161, 171
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 1 cut(s) 143
MvaI CCWGG 1 cut(s) 143
NdeII GATC 3 cut(s) 195, 201, 262
NlaIII CATG 2 cut(s) 191, 226
NlaIV GGNNCC 2 cut(s) 68, 203
NmuCI GTSAC 1 cut(s) 168
PagI TCATGA 1 cut(s) 222
PfoI TCCNGGA 1 cut(s) 141
PkrI GCNGC 1 cut(s) 18
PsiI TTATAA 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 2 cut(s) 68, 203
PsuI RGATCY 1 cut(s) 201
RseI CAYNNNNRTG 1 cut(s) 11
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 1 cut(s) 17
Sau3AI GATC 3 cut(s) 195, 201, 262
ScrFI CCNGG 1 cut(s) 143
SetI ASST 3 cut(s) 84, 153, 276
SmiMI CAYNNNNRTG 1 cut(s) 11
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 3 cut(s) 50, 59, 121
SsiI CCGC 1 cut(s) 205
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 1 cut(s) 141
TasI AATT 3 cut(s) 50, 59, 121
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseFI GTSAC 1 cut(s) 168
TseI GCWGC 1 cut(s) 16
Tsp45I GTSAC 1 cut(s) 168
TspDTI ATGAA 2 cut(s) 62, 239
XapI RAATTY 3 cut(s) 50, 59, 121
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.