RLG00000022536

MOS2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013642
Physical Location & Seq
Forward (+)
3000128 .. 3002120
1993 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022536

Sequence Viewer

Length: 612 bp
ATGTCGGACAGTCGGCTAGCTGCAATGATGTCAATGTCCGGAATGCAAAAAATTTTAACAAATGAGTTTCCTAATCCAGAATTGAAGCGCCCTGTTCAATTCGACCCGTTCACTAGCGTATTTGACACTCTGAAGAAGCTTCCAAACCCTATCAAACCCTCCACTTCCTTCGAAGACGACGACATATCGAAACAATACATCCACGAATTCACCGCCGCGGAAGAGCCCTCAGCCAAACCCCACGTGATCCCGCCCATCCCAAACCAATGGCAGCCCGAGAAGAACACTATGAACAACCTCGAATTCGTGGCCGAGTCTCTCTCTATCGACGGTTCAAATTCCGAGATCCCGTACGGCCTCAATCTTCGACAGAGATCGGAATCTGACGAGGCGGTGGTTCAGAAATCGAAAGAGGAATTGAAGGCGTTGTCGGAGTTCAGGGGGATGGAGGAGTTTGATGAGAATCCGGTGGAGGGGTTCGGTGCGGCTCTGCTCACCGGGTACGAGTGGCAACCGGGGCGAGGAATCGGCAAGAATGCAAAGGAAGTTGTAGGTGTTGTGGAGGTCCGGCCCAATGTGGGAAGCCAAGGGTTAGGGTTTCGACCGAAATAG

Protein Analysis

204

Amino Acids

22.63

Weight (kDa)

4.88

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch_2 PF12656 148 - 202 7e-14 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 218
AccIII TCCGGA 1 cut(s) 38
AciI CCGC 6 cut(s) 213, 216, 218, 251, 392, 485
AclWI GGATC 2 cut(s) 241, 340
AcoI YGGCCR 1 cut(s) 309
AcsI RAATTY 4 cut(s) 51, 206, 302, 337
AcuI CTGAAG 1 cut(s) 152
AcvI CACGTG 1 cut(s) 244
AfaI GTAC 2 cut(s) 353, 503
AfiI CCNNNNNNNGG 3 cut(s) 473, 521, 578
AgsI TTSAA 4 cut(s) 85, 98, 336, 421
AluBI AGCT 2 cut(s) 20, 139
AluI AGCT 2 cut(s) 20, 139
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 2 cut(s) 241, 340
Ama87I CYCGRG 1 cut(s) 275
Aor13HI TCCGGA 1 cut(s) 38
AoxI GGCC 3 cut(s) 309, 355, 569
ApeKI GCWGC 2 cut(s) 20, 271
ApoI RAATTY 4 cut(s) 51, 206, 302, 337
AspLEI GCGC 1 cut(s) 90
AspS9I GGNCC 2 cut(s) 565, 570
AsuC2I CCSGG 2 cut(s) 499, 516
AsuHPI GGTGA 2 cut(s) 202, 487
AsuII TTCGAA 1 cut(s) 171
AsuNHI GCTAGC 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 228
BbrPI CACGTG 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 180
BbvCI CCTCAGC 1 cut(s) 229
BbvI GCAGC 2 cut(s) 7, 283
BccI CCATC 2 cut(s) 263, 439
BceAI ACGGC 1 cut(s) 370
BcnI CCSGG 2 cut(s) 499, 516
BcoDI GTCTC 1 cut(s) 321
BfaI CTAG 2 cut(s) 17, 114
BfoI RGCGCY 1 cut(s) 91
BisI GCNGC 4 cut(s) 21, 216, 272, 486
BlsI GCNGC 4 cut(s) 22, 217, 273, 487
Bme1390I CCNGG 2 cut(s) 499, 516
Bme18I GGWCC 1 cut(s) 565
BmeT110I CYCGRG 1 cut(s) 275
BmgT120I GGNCC 2 cut(s) 565, 570
BmrFI CCNGG 2 cut(s) 499, 516
BmtI GCTAGC 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 180
BplI GAGNNNNNCTC 2 cut(s) 305, 337
Bpu10I CCTNAGC 1 cut(s) 229
Bpu14I TTCGAA 1 cut(s) 171
BpuMI CCSGG 2 cut(s) 499, 516
BsaAI YACGTR 1 cut(s) 244
BsaBI GATNNNNATC 2 cut(s) 379, 462
BsaJI CCNNGG 3 cut(s) 216, 515, 586
BsaWI WCCGGW 2 cut(s) 38, 466
Bsc4I CCNNNNNNNGG 3 cut(s) 473, 521, 578
Bse3DI GCAATG 1 cut(s) 30
Bse8I GATNNNNATC 2 cut(s) 379, 462
BseAI TCCGGA 1 cut(s) 38
BseDI CCNNGG 3 cut(s) 216, 515, 586
BseGI GGATG 3 cut(s) 198, 255, 450
BseJI GATNNNNATC 2 cut(s) 379, 462
BseLI CCNNNNNNNGG 3 cut(s) 473, 521, 578
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 1 cut(s) 243
BseRI GAGGAG 1 cut(s) 464
BseXI GCAGC 2 cut(s) 7, 283
Bsh1236I CGCG 1 cut(s) 218
Bsh1285I CGRYCG 1 cut(s) 605
BshFI GGCC 3 cut(s) 311, 357, 571
BsiEI CGRYCG 1 cut(s) 605
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 5 cut(s) 39, 467, 498, 515, 568
BsiWI CGTACG 1 cut(s) 351
BslI CCNNNNNNNGG 3 cut(s) 473, 521, 578
BsmAI GTCTC 1 cut(s) 321
BsmI GAATGC 2 cut(s) 48, 541
BsnI GGCC 3 cut(s) 311, 357, 571
BsoBI CYCGRG 1 cut(s) 275
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 228
Bsp13I TCCGGA 1 cut(s) 38
Bsp143I GATC 3 cut(s) 246, 345, 374
BspACI CCGC 6 cut(s) 213, 216, 218, 251, 392, 485
BspANI GGCC 3 cut(s) 311, 357, 571
BspCNI CTCAG 1 cut(s) 242
BspEI TCCGGA 1 cut(s) 38
BspFNI CGCG 1 cut(s) 218
BspOI GCTAGC 1 cut(s) 20
BspPI GGATC 2 cut(s) 241, 340
BspQI GCTCTTC 1 cut(s) 216
BspT104I TTCGAA 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 30
BssECI CCNNGG 3 cut(s) 216, 515, 586
BssMI GATC 3 cut(s) 246, 345, 374
BssT1I CCWWGG 1 cut(s) 586
Bst4CI ACNGT 2 cut(s) 11, 332
Bst6I CTCTTC 1 cut(s) 216
BstBAI YACGTR 1 cut(s) 244
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 1 cut(s) 229
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 3 cut(s) 198, 255, 450
BstFNI CGCG 1 cut(s) 218
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 3 cut(s) 249, 348, 377
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 3 cut(s) 246, 345, 374
BstMCI CGRYCG 1 cut(s) 605
BstMWI GCNNNNNNNGC 1 cut(s) 517
BstSCI CCNGG 2 cut(s) 497, 514
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 2 cut(s) 7, 283
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 345
BstXI CCANNNNNNTGG 1 cut(s) 267
BstYI RGATCY 1 cut(s) 345
BsuRI GGCC 3 cut(s) 311, 357, 571
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 3 cut(s) 198, 255, 450
Cac8I GCNNGC 1 cut(s) 18
CfoI GCGC 1 cut(s) 90
Cfr13I GGNCC 2 cut(s) 565, 570
Cfr42I CCGCGG 1 cut(s) 219
Csp6I GTAC 2 cut(s) 352, 502
CviQI GTAC 2 cut(s) 352, 502
DdeI CTNAG 1 cut(s) 229
DpnI GATC 3 cut(s) 248, 347, 376
DpnII GATC 3 cut(s) 246, 345, 374
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 586
Eco24I GRGCYC 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 565
Eco57I CTGAAG 1 cut(s) 152
Eco72I CACGTG 1 cut(s) 244
Eco88I CYCGRG 1 cut(s) 275
EcoRI GAATTC 2 cut(s) 206, 302
EcoT14I CCWWGG 1 cut(s) 586
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 586
FaiI YATR 2 cut(s) 185, 290
FauI CCCGC 1 cut(s) 258
Fnu4HI GCNGC 4 cut(s) 21, 216, 272, 486
FokI GGATG 3 cut(s) 185, 242, 457
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 4 cut(s) 21, 216, 272, 486
FspBI CTAG 2 cut(s) 17, 114
GlaI GCGC 1 cut(s) 89
GluI GCNGC 4 cut(s) 21, 216, 272, 486
HaeII RGCGCY 1 cut(s) 91
HaeIII GGCC 3 cut(s) 311, 357, 571
HapII CCGG 5 cut(s) 39, 467, 498, 515, 568
HhaI GCGC 1 cut(s) 90
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 4 cut(s) 314, 380, 463, 525
HpaII CCGG 5 cut(s) 39, 467, 498, 515, 568
HphI GGTGA 2 cut(s) 202, 487
Hpy166II GTNNAC 1 cut(s) 111
Hpy188I TCNGA 7 cut(s) 7, 132, 343, 379, 385, 402, 433
Hpy188III TCNNGA 2 cut(s) 39, 77
Hpy8I GTNNAC 1 cut(s) 111
Hpy99I CGWCG 2 cut(s) 182, 332
HpyAV CCTTC 2 cut(s) 178, 415
HpyCH4III ACNGT 2 cut(s) 11, 332
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 3 cut(s) 23, 46, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 517
HpyF3I CTNAG 1 cut(s) 229
HpySE526I ACGT 1 cut(s) 243
HspAI GCGC 1 cut(s) 88
Kpn2I TCCGGA 1 cut(s) 38
KspI CCGCGG 1 cut(s) 219
Kzo9I GATC 3 cut(s) 246, 345, 374
LguI GCTCTTC 1 cut(s) 216
LpnPI CCDG 8 cut(s) 52, 90, 105, 424, 480, 511, 528, 581
Lsp1109I GCAGC 2 cut(s) 7, 283
MaeI CTAG 2 cut(s) 17, 114
MaeII ACGT 1 cut(s) 243
MalI GATC 3 cut(s) 248, 347, 376
MboI GATC 3 cut(s) 246, 345, 374
MboII GAAGA 5 cut(s) 145, 185, 233, 292, 356
MflI RGATCY 1 cut(s) 345
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 7 cut(s) 51, 80, 98, 206, 302, 337, 416
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 1 cut(s) 411
MroI TCCGGA 1 cut(s) 38
MseI TTAA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 5 cut(s) 39, 467, 498, 515, 568
MspR9I CCNGG 2 cut(s) 499, 516
Mva1269I GAATGC 2 cut(s) 48, 541
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 517
NciI CCSGG 2 cut(s) 499, 516
NdeII GATC 3 cut(s) 246, 345, 374
NheI GCTAGC 1 cut(s) 16
NmeAIII GCCGAG 1 cut(s) 337
NspV TTCGAA 1 cut(s) 171
PciSI GCTCTTC 1 cut(s) 216
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 2 cut(s) 48, 541
PfeI GAWTC 3 cut(s) 380, 463, 525
Pfl23II CGTACG 1 cut(s) 351
PkrI GCNGC 4 cut(s) 22, 217, 273, 487
PleI GAGTC 1 cut(s) 322
PmaCI CACGTG 1 cut(s) 244
PmlI CACGTG 1 cut(s) 244
PpsI GAGTC 1 cut(s) 322
Ppu21I YACGTR 1 cut(s) 244
PspCI CACGTG 1 cut(s) 244
PspLI CGTACG 1 cut(s) 351
PspPI GGNCC 2 cut(s) 565, 570
PsuI RGATCY 1 cut(s) 345
RsaI GTAC 2 cut(s) 353, 503
RsaNI GTAC 2 cut(s) 352, 502
SacII CCGCGG 1 cut(s) 219
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 4 cut(s) 21, 216, 272, 486
Sau3AI GATC 3 cut(s) 246, 345, 374
Sau96I GGNCC 2 cut(s) 565, 570
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 2 cut(s) 499, 516
SduI GDGCHC 1 cut(s) 228
SetI ASST 6 cut(s) 22, 141, 246, 300, 556, 567
Sfr303I CCGCGG 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 171
SgrBI CCGCGG 1 cut(s) 219
SinI GGWCC 1 cut(s) 565
Sse9I AATT 7 cut(s) 51, 80, 98, 206, 302, 337, 416
SsiI CCGC 6 cut(s) 213, 216, 218, 251, 392, 485
SspMI CTAG 2 cut(s) 17, 114
StyD4I CCNGG 2 cut(s) 497, 514
StyI CCWWGG 1 cut(s) 586
TaaI ACNGT 2 cut(s) 11, 332
TaiI ACGT 1 cut(s) 246
TaqI TCGA 8 cut(s) 102, 171, 188, 300, 327, 367, 407, 601
TasI AATT 7 cut(s) 51, 80, 98, 206, 302, 337, 416
TauI GCSGC 2 cut(s) 218, 488
TfiI GAWTC 3 cut(s) 380, 463, 525
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TseI GCWGC 2 cut(s) 20, 271
TspDTI ATGAA 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 565
XapI RAATTY 4 cut(s) 51, 206, 302, 337
XspI CTAG 2 cut(s) 17, 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.