Rh5DG270400

MOS2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013642
Physical Location & Seq
Forward (+)
32915981 .. 32916526
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG270400.1

Sequence Viewer

Length: 546 bp
ATGAGAATCTCTTTCTCTCTGCCTTCAAACTCCTCCTCGAAGCCAAACCCAGTCAAACCCTCCACTTCCTTCGAAGACGACGACGACGACACCACTACCCCCAACCTAAACGACGACGCATCGAAACAATACATCCACGAATTCACCGCCGCGGAAGAGCCCTCAGCCAAACCCCGCGTGATCCCGCCCATCCCAAACGAATGGCAGCCGGGGAAGAACACCGTGAACAGCCTCGAATTCGTGGCCGAGTCTCTCTCAATCGACGGCTCATATTCCGAGATCCCGTACGGCCTCAATCTCCGGCAGAGACCCGAATCCGAGGAGGCGGTGGTTCAGAAATTGAGAGAGGAATTGAAGGCGTTGCCGGAGATTAGGGGGATGGAGGAGTTTGATGAGAATCCGGTGGAGGGGTTCGGTGCGGCTCTGCTCGCCGGGTACGGGTGGCAACCGGGGCGAGGAATCGGCAAGAACGCAAAGGAAGATGTTGGTGTTGTGGAGGTCCGGCCCAATGTGGGAAGGCAAGGGTTAGGGTTTCGGCCGGAATAG

Protein Analysis

181

Amino Acids

19.91

Weight (kDa)

4.63

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch_2 PF12656 122 - 180 2.6e-16 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 152, 177
AciI CCGC 7 cut(s) 147, 150, 152, 175, 185, 326, 419
AclWI GGATC 2 cut(s) 175, 274
AcoI YGGCCR 2 cut(s) 243, 536
AcsI RAATTY 2 cut(s) 140, 236
AfaI GTAC 2 cut(s) 287, 437
AfiI CCNNNNNNNGG 4 cut(s) 407, 438, 455, 512
AgsI TTSAA 2 cut(s) 27, 355
Alw26I GTCTC 2 cut(s) 255, 301
AlwI GGATC 2 cut(s) 175, 274
AoxI GGCC 4 cut(s) 243, 289, 503, 536
ApeKI GCWGC 1 cut(s) 205
ApoI RAATTY 2 cut(s) 140, 236
AspS9I GGNCC 2 cut(s) 499, 504
AsuC2I CCSGG 3 cut(s) 210, 433, 450
AsuHPI GGTGA 1 cut(s) 136
AsuII TTCGAA 1 cut(s) 72
AvaII GGWCC 1 cut(s) 499
BanII GRGCYC 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 81
BbvCI CCTCAGC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 217
BccI CCATC 2 cut(s) 197, 373
BceAI ACGGC 2 cut(s) 280, 304
BcnI CCSGG 3 cut(s) 210, 433, 450
BcoDI GTCTC 2 cut(s) 255, 301
BisI GCNGC 3 cut(s) 150, 206, 420
BlsI GCNGC 3 cut(s) 151, 207, 421
Bme1390I CCNGG 3 cut(s) 210, 433, 450
Bme18I GGWCC 1 cut(s) 499
BmgT120I GGNCC 2 cut(s) 499, 504
BmrFI CCNGG 3 cut(s) 210, 433, 450
BmrI ACTGGG 1 cut(s) 44
BmsI GCATC 1 cut(s) 128
BmuI ACTGGG 1 cut(s) 44
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 239, 271
Bpu10I CCTNAGC 1 cut(s) 163
Bpu14I TTCGAA 1 cut(s) 72
BpuMI CCSGG 3 cut(s) 210, 433, 450
BsaBI GATNNNNATC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 301
BsaJI CCNNGG 4 cut(s) 150, 209, 318, 449
BsaWI WCCGGW 1 cut(s) 400
Bsc4I CCNNNNNNNGG 4 cut(s) 407, 438, 455, 512
Bse1I ACTGG 1 cut(s) 50
Bse8I GATNNNNATC 1 cut(s) 396
BseDI CCNNGG 4 cut(s) 150, 209, 318, 449
BseGI GGATG 3 cut(s) 132, 189, 384
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 4 cut(s) 407, 438, 455, 512
BseMII CTCAG 1 cut(s) 177
BseNI ACTGG 1 cut(s) 50
BseRI GAGGAG 4 cut(s) 22, 25, 335, 398
BseX3I CGGCCG 1 cut(s) 536
BseXI GCAGC 1 cut(s) 217
Bsh1236I CGCG 2 cut(s) 152, 177
Bsh1285I CGRYCG 1 cut(s) 539
BshFI GGCC 4 cut(s) 245, 291, 505, 538
BsiEI CGRYCG 1 cut(s) 539
BsiSI CCGG 8 cut(s) 209, 301, 365, 401, 432, 449, 502, 539
BsiWI CGTACG 1 cut(s) 285
BslI CCNNNNNNNGG 4 cut(s) 407, 438, 455, 512
BsmAI GTCTC 2 cut(s) 255, 301
BsnI GGCC 4 cut(s) 245, 291, 505, 538
Bso31I GGTCTC 1 cut(s) 301
Bsp119I TTCGAA 1 cut(s) 72
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 2 cut(s) 180, 279
BspACI CCGC 7 cut(s) 147, 150, 152, 175, 185, 326, 419
BspANI GGCC 4 cut(s) 245, 291, 505, 538
BspCNI CTCAG 1 cut(s) 176
BspFNI CGCG 2 cut(s) 152, 177
BspPI GGATC 2 cut(s) 175, 274
BspQI GCTCTTC 1 cut(s) 150
BspT104I TTCGAA 1 cut(s) 72
BspTNI GGTCTC 1 cut(s) 301
BsrI ACTGG 1 cut(s) 50
BssECI CCNNGG 4 cut(s) 150, 209, 318, 449
BssMI GATC 2 cut(s) 180, 279
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 1 cut(s) 163
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 3 cut(s) 132, 189, 384
BstFNI CGCG 2 cut(s) 152, 177
BstKTI GATC 2 cut(s) 183, 282
BstMAI GTCTC 2 cut(s) 255, 301
BstMBI GATC 2 cut(s) 180, 279
BstMCI CGRYCG 1 cut(s) 539
BstMWI GCNNNNNNNGC 2 cut(s) 428, 451
BstSCI CCNGG 3 cut(s) 208, 431, 448
BstUI CGCG 2 cut(s) 152, 177
BstV1I GCAGC 1 cut(s) 217
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 279
BstXI CCANNNNNNTGG 1 cut(s) 201
BstYI RGATCY 1 cut(s) 279
BstZI CGGCCG 1 cut(s) 536
BsuRI GGCC 4 cut(s) 245, 291, 505, 538
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 3 cut(s) 132, 189, 384
Cac8I GCNNGC 1 cut(s) 429
Cfr13I GGNCC 2 cut(s) 499, 504
Cfr42I CCGCGG 1 cut(s) 153
CseI GACGC 1 cut(s) 125
Csp6I GTAC 2 cut(s) 286, 436
CviQI GTAC 2 cut(s) 286, 436
DdeI CTNAG 1 cut(s) 163
DpnI GATC 2 cut(s) 182, 281
DpnII GATC 2 cut(s) 180, 279
EaeI YGGCCR 2 cut(s) 243, 536
EagI CGGCCG 1 cut(s) 536
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EclXI CGGCCG 1 cut(s) 536
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 301
Eco47I GGWCC 1 cut(s) 499
Eco52I CGGCCG 1 cut(s) 536
EcoRI GAATTC 2 cut(s) 140, 236
EcoT38I GRGCYC 1 cut(s) 162
FaiI YATR 1 cut(s) 271
FauI CCCGC 2 cut(s) 182, 192
Fnu4HI GCNGC 3 cut(s) 150, 206, 420
FokI GGATG 3 cut(s) 119, 176, 391
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 3 cut(s) 150, 206, 420
GluI GCNGC 3 cut(s) 150, 206, 420
HaeIII GGCC 4 cut(s) 245, 291, 505, 538
HapII CCGG 8 cut(s) 209, 301, 365, 401, 432, 449, 502, 539
HgaI GACGC 1 cut(s) 125
HinfI GANTC 5 cut(s) 6, 248, 314, 397, 459
HpaII CCGG 8 cut(s) 209, 301, 365, 401, 432, 449, 502, 539
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 3 cut(s) 277, 319, 336
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 6 cut(s) 83, 86, 89, 116, 119, 266
HpyAV CCTTC 4 cut(s) 33, 79, 349, 510
HpyCH4III ACNGT 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 2 cut(s) 428, 451
HpyF3I CTNAG 1 cut(s) 163
KspI CCGCGG 1 cut(s) 153
Kzo9I GATC 2 cut(s) 180, 279
LguI GCTCTTC 1 cut(s) 150
LpnPI CCDG 8 cut(s) 63, 222, 314, 378, 414, 445, 462, 515
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 1 cut(s) 128
MalI GATC 2 cut(s) 182, 281
MboI GATC 2 cut(s) 180, 279
MboII GAAGA 4 cut(s) 86, 167, 226, 491
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 162
MluCI AATT 4 cut(s) 140, 236, 338, 350
MlyI GAGTC 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 8 cut(s) 209, 301, 365, 401, 432, 449, 502, 539
MspR9I CCNGG 3 cut(s) 210, 433, 450
MvnI CGCG 2 cut(s) 152, 177
MwoI GCNNNNNNNGC 2 cut(s) 428, 451
NciI CCSGG 3 cut(s) 210, 433, 450
NdeII GATC 2 cut(s) 180, 279
NmeAIII GCCGAG 1 cut(s) 271
NspV TTCGAA 1 cut(s) 72
PciSI GCTCTTC 1 cut(s) 150
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 84
PfeI GAWTC 4 cut(s) 6, 314, 397, 459
Pfl23II CGTACG 1 cut(s) 285
PkrI GCNGC 3 cut(s) 151, 207, 421
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
PspLI CGTACG 1 cut(s) 285
PspPI GGNCC 2 cut(s) 499, 504
PsuI RGATCY 1 cut(s) 279
RsaI GTAC 2 cut(s) 287, 437
RsaNI GTAC 2 cut(s) 286, 436
SacII CCGCGG 1 cut(s) 153
SapI GCTCTTC 1 cut(s) 150
SatI GCNGC 3 cut(s) 150, 206, 420
Sau3AI GATC 2 cut(s) 180, 279
Sau96I GGNCC 2 cut(s) 499, 504
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 3 cut(s) 210, 433, 450
SduI GDGCHC 1 cut(s) 162
SetI ASST 2 cut(s) 108, 501
SfaNI GCATC 1 cut(s) 128
Sfr303I CCGCGG 1 cut(s) 153
SfuI TTCGAA 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 153
SinI GGWCC 1 cut(s) 499
Sse9I AATT 4 cut(s) 140, 236, 338, 350
SsiI CCGC 7 cut(s) 147, 150, 152, 175, 185, 326, 419
StyD4I CCNGG 3 cut(s) 208, 431, 448
TaaI ACNGT 1 cut(s) 223
TaqI TCGA 5 cut(s) 38, 72, 122, 234, 261
TasI AATT 4 cut(s) 140, 236, 338, 350
TauI GCSGC 2 cut(s) 152, 422
TfiI GAWTC 4 cut(s) 6, 314, 397, 459
TseI GCWGC 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 499
XapI RAATTY 2 cut(s) 140, 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.