RLG00000033830

MOS2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013642
Physical Location & Seq
Forward (+)
32764600 .. 32765685
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033830

Sequence Viewer

Length: 549 bp
ATGAGAATCTCTTTCTCTCTGCCTCCAAACTCCTCCTCAAAGCCAAACCCTATCAAACCCTCAACCTCCTTTGAAGACGACGACGACACCACCAACCCCAACCCCAACCTAAACGACGACGCATCGAAACAATACATCCACGAATTCACCGCCGCGGAAGAGCCCTCAGCCAAACCCCGCGTGATCCCGCCCATCCCAAACGAATGGCAGCCCGGCAAGAACACCATGAACAACCTCGAATTCGTGGCCGAGTCTCTCTCTATCGACGGCTCATATTCCGAGATCCCGTACGGCCTCAATCTCCGGCAGAGACCCGAATCCGAGGAGGTGGTGGTTCAGAAATTGAAAGAGGAATTGAAGGCGTTGCCGGAGATTAGGGGGATGGAGGAGTTTGATGAGAATCCGGTGGAGGGGTTCGGTGCGGCTCTGCTCGCCGGGTACGGGTGGCAACCGGGGCGAGGAATCGGCAAGAACGCAAAGGAAGATGTTGGTGTTGTGGAGGTCCGGCCCAATGTGGGAAGGCAAGGGTTAGGGTTTCGACCGGAATAG

Protein Analysis

183

Amino Acids

20.11

Weight (kDa)

4.68

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch_2 PF12656 123 - 181 2.7e-16 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 155, 180
AciI CCGC 6 cut(s) 150, 153, 155, 178, 188, 422
AclWI GGATC 2 cut(s) 178, 277
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 2 cut(s) 143, 239
AfaI GTAC 2 cut(s) 290, 440
AfiI CCNNNNNNNGG 4 cut(s) 410, 441, 458, 515
AgsI TTSAA 3 cut(s) 74, 346, 358
Alw26I GTCTC 2 cut(s) 258, 304
AlwI GGATC 2 cut(s) 178, 277
AoxI GGCC 3 cut(s) 246, 292, 506
ApeKI GCWGC 1 cut(s) 208
ApoI RAATTY 2 cut(s) 143, 239
AspS9I GGNCC 2 cut(s) 502, 507
AsuC2I CCSGG 3 cut(s) 213, 436, 453
AsuHPI GGTGA 1 cut(s) 139
AvaII GGWCC 1 cut(s) 502
BanII GRGCYC 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 81
BbvCI CCTCAGC 1 cut(s) 166
BbvI GCAGC 1 cut(s) 220
BccI CCATC 2 cut(s) 200, 376
BceAI ACGGC 2 cut(s) 283, 307
BcnI CCSGG 3 cut(s) 213, 436, 453
BcoDI GTCTC 2 cut(s) 258, 304
BisI GCNGC 3 cut(s) 153, 209, 423
BlsI GCNGC 3 cut(s) 154, 210, 424
Bme1390I CCNGG 3 cut(s) 213, 436, 453
Bme18I GGWCC 1 cut(s) 502
BmgT120I GGNCC 2 cut(s) 502, 507
BmrFI CCNGG 3 cut(s) 213, 436, 453
BmsI GCATC 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 242, 274
Bpu10I CCTNAGC 1 cut(s) 166
BpuMI CCSGG 3 cut(s) 213, 436, 453
BsaBI GATNNNNATC 1 cut(s) 399
BsaI GGTCTC 1 cut(s) 304
BsaJI CCNNGG 3 cut(s) 153, 321, 452
BsaWI WCCGGW 2 cut(s) 403, 541
Bsc4I CCNNNNNNNGG 4 cut(s) 410, 441, 458, 515
Bse8I GATNNNNATC 1 cut(s) 399
BseDI CCNNGG 3 cut(s) 153, 321, 452
BseGI GGATG 3 cut(s) 135, 192, 387
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 4 cut(s) 410, 441, 458, 515
BseMII CTCAG 1 cut(s) 180
BseRI GAGGAG 4 cut(s) 22, 25, 338, 401
BseXI GCAGC 1 cut(s) 220
Bsh1236I CGCG 2 cut(s) 155, 180
Bsh1285I CGRYCG 1 cut(s) 542
BshFI GGCC 3 cut(s) 248, 294, 508
BsiEI CGRYCG 1 cut(s) 542
BsiSI CCGG 8 cut(s) 213, 304, 368, 404, 435, 452, 505, 542
BsiWI CGTACG 1 cut(s) 288
BslI CCNNNNNNNGG 4 cut(s) 410, 441, 458, 515
BsmAI GTCTC 2 cut(s) 258, 304
BsnI GGCC 3 cut(s) 248, 294, 508
Bso31I GGTCTC 1 cut(s) 304
Bsp1286I GDGCHC 1 cut(s) 165
Bsp143I GATC 2 cut(s) 183, 282
BspACI CCGC 6 cut(s) 150, 153, 155, 178, 188, 422
BspANI GGCC 3 cut(s) 248, 294, 508
BspCNI CTCAG 1 cut(s) 179
BspFNI CGCG 2 cut(s) 155, 180
BspPI GGATC 2 cut(s) 178, 277
BspQI GCTCTTC 1 cut(s) 153
BspTNI GGTCTC 1 cut(s) 304
BssECI CCNNGG 3 cut(s) 153, 321, 452
BssMI GATC 2 cut(s) 183, 282
Bst6I CTCTTC 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 1 cut(s) 166
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 135, 192, 387
BstFNI CGCG 2 cut(s) 155, 180
BstKTI GATC 2 cut(s) 186, 285
BstMAI GTCTC 2 cut(s) 258, 304
BstMBI GATC 2 cut(s) 183, 282
BstMCI CGRYCG 1 cut(s) 542
BstMWI GCNNNNNNNGC 2 cut(s) 431, 454
BstSCI CCNGG 3 cut(s) 211, 434, 451
BstUI CGCG 2 cut(s) 155, 180
BstV1I GCAGC 1 cut(s) 220
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 282
BstXI CCANNNNNNTGG 1 cut(s) 204
BstYI RGATCY 1 cut(s) 282
BsuRI GGCC 3 cut(s) 248, 294, 508
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 135, 192, 387
Cac8I GCNNGC 1 cut(s) 432
Cfr13I GGNCC 2 cut(s) 502, 507
Cfr42I CCGCGG 1 cut(s) 156
CseI GACGC 1 cut(s) 128
Csp6I GTAC 2 cut(s) 289, 439
CviAII CATG 1 cut(s) 226
CviJI RGCY 9 cut(s) 43, 163, 170, 211, 248, 270, 294, 425, 508
CviKI_1 RGCY 9 cut(s) 43, 163, 170, 211, 248, 270, 294, 425, 508
CviQI GTAC 2 cut(s) 289, 439
DdeI CTNAG 1 cut(s) 166
DpnI GATC 2 cut(s) 185, 284
DpnII GATC 2 cut(s) 183, 282
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 165
Eco31I GGTCTC 1 cut(s) 304
Eco47I GGWCC 1 cut(s) 502
EcoRI GAATTC 2 cut(s) 143, 239
EcoT38I GRGCYC 1 cut(s) 165
FaeI CATG 1 cut(s) 229
FaiI YATR 2 cut(s) 227, 274
FatI CATG 1 cut(s) 225
FauI CCCGC 2 cut(s) 185, 195
Fnu4HI GCNGC 3 cut(s) 153, 209, 423
FokI GGATG 3 cut(s) 122, 179, 394
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 3 cut(s) 153, 209, 423
GluI GCNGC 3 cut(s) 153, 209, 423
HaeIII GGCC 3 cut(s) 248, 294, 508
HapII CCGG 8 cut(s) 213, 304, 368, 404, 435, 452, 505, 542
HgaI GACGC 1 cut(s) 128
Hin1II CATG 1 cut(s) 229
HinfI GANTC 5 cut(s) 6, 251, 317, 400, 462
HpaII CCGG 8 cut(s) 213, 304, 368, 404, 435, 452, 505, 542
HphI GGTGA 1 cut(s) 139
Hpy188I TCNGA 3 cut(s) 280, 322, 339
Hpy99I CGWCG 5 cut(s) 83, 86, 119, 122, 269
HpyAV CCTTC 2 cut(s) 352, 513
HpyF10VI GCNNNNNNNGC 2 cut(s) 431, 454
HpyF3I CTNAG 1 cut(s) 166
Hsp92II CATG 1 cut(s) 229
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 2 cut(s) 183, 282
LguI GCTCTTC 1 cut(s) 153
LpnPI CCDG 7 cut(s) 226, 317, 381, 417, 448, 465, 518
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 1 cut(s) 131
MalI GATC 2 cut(s) 185, 284
MboI GATC 2 cut(s) 183, 282
MboII GAAGA 3 cut(s) 86, 170, 494
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 4 cut(s) 143, 239, 341, 353
MlyI GAGTC 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 8 cut(s) 213, 304, 368, 404, 435, 452, 505, 542
MspR9I CCNGG 3 cut(s) 213, 436, 453
MvnI CGCG 2 cut(s) 155, 180
MwoI GCNNNNNNNGC 2 cut(s) 431, 454
NciI CCSGG 3 cut(s) 213, 436, 453
NdeII GATC 2 cut(s) 183, 282
NlaIII CATG 1 cut(s) 229
NmeAIII GCCGAG 1 cut(s) 274
PciSI GCTCTTC 1 cut(s) 153
PfeI GAWTC 4 cut(s) 6, 317, 400, 462
Pfl23II CGTACG 1 cut(s) 288
PkrI GCNGC 3 cut(s) 154, 210, 424
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PspLI CGTACG 1 cut(s) 288
PspPI GGNCC 2 cut(s) 502, 507
PsuI RGATCY 1 cut(s) 282
RsaI GTAC 2 cut(s) 290, 440
RsaNI GTAC 2 cut(s) 289, 439
SacII CCGCGG 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 153
SatI GCNGC 3 cut(s) 153, 209, 423
Sau3AI GATC 2 cut(s) 183, 282
Sau96I GGNCC 2 cut(s) 502, 507
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 3 cut(s) 213, 436, 453
SduI GDGCHC 1 cut(s) 165
SetI ASST 5 cut(s) 68, 111, 237, 330, 504
SfaNI GCATC 1 cut(s) 131
Sfr303I CCGCGG 1 cut(s) 156
SgrBI CCGCGG 1 cut(s) 156
SinI GGWCC 1 cut(s) 502
Sse9I AATT 4 cut(s) 143, 239, 341, 353
SsiI CCGC 6 cut(s) 150, 153, 155, 178, 188, 422
StyD4I CCNGG 3 cut(s) 211, 434, 451
TaqI TCGA 4 cut(s) 125, 237, 264, 538
TasI AATT 4 cut(s) 143, 239, 341, 353
TauI GCSGC 2 cut(s) 155, 425
TfiI GAWTC 4 cut(s) 6, 317, 400, 462
TseI GCWGC 1 cut(s) 208
TspDTI ATGAA 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 502
XapI RAATTY 2 cut(s) 143, 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.