Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0002712

Physical Location & Seq

Genomic Coordinates Forward (+)

19976947 .. 19985362

Gene Structure 8416 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023209

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGATGCAAATCACATTATTTAACATCTCTTCCTTCTTCAGCTGTGTTATCTTGTTTCCTGATGGGTTGCAACATCCATGGCTGAAACTATCTTGCCAAAAAGAGGCTAGAGAGCCTGATGTTGAAGGTGATGCATTAATCGATTTGCTTAAGGCACTGAATGATTCCAATGGCCGAATAACTGATTGGGATATTCATCTTGTGAGCCCATGTTTTAGCTGGTCTCATGTCACTTGTAGAAATGGAAATGTCATATCCCTGAGCCTGGCATCAAATGGATTTTCTGGAACACTTTCTCCTTCCATTACCAAACTCAAATTTTTGTCCAGCCTGGACTTCCAGGATAATAGTCTAACGGGTCTGTTACCCGTTTATCTTGCCAACTTGACACATCTCCAGAATCTAAACTTTGCAAATAATAACTTCAGAGGTCCTATACCCAATACTTGGGGTCAACTTTCCAATCTAAAGCACTTGGTTTTGAGGGGTAATGACATCTCTGGTGATATTCCGGATTCACTTTCTAATATCACAGGGTTGGCTGAATTGGATCTTTCATCTAATGATTTAGAGGGAAAAATTCCAATGCAACTCTTCACGGTTCCGACTTTCAATTTTACAGGAACTCATCTTACTTGTGGTTCTAGCTTGAACCAGCCTTGTGCTTCTGGCTCTTCACTCGTTTCAACCAAGAAATCAAAACTTGGAAGAGTTATAACTTTGGCAAGTTGTGGTGTGACAATACTTCTTTTTATCGGGGCTATTTTTGGATATCGATATTATCACATGCACGGTCTGAAGCATGAGGTGTTTGTTGATGTTGCGGGTGAAGATGACTGTAAAATTTCCTTTGGTCAATTGAGAAGATTTTCTTGGCGTGAAATTCAAATTGCCACAGATAATTTCGATGAGAGTAACATAATTGGACAGGGTGGCTTTGGAAGAGTTTACAGAGGTGTTCTCTCAGACAATGTAAAAATTGCAGTGAAACGCCTTACCGACTATAACAGTCCTGGTGGAGAGGCTGCATTCCTGAGAGAAGTACAACTCATTAGTGTTGCAGTTCATAGGAATCTTTTACGGTTGATTGGCTTTTGTACAACTTCATCTGAGAGGGTCCTTGTTTATCCGTTCATGAAAAACCTGAGTGTTGCGTATCGCTTGAGAGATCTGAAACCAGGAGAGAAAGGCTTGGACTGGTCAACAAGGAAGCGCATTGCTTTTGGTGCAGCCCATGGCTTGGAGTATCTACATGAGTATTGCAATCCTAAGATTATACACCGTGACTTGAAGGCTGCAAACATTCTTCTAGATGATTATTTTGAGCCTGTTCTTGGAGATTTTGGGCTAGCACAGCTGGTCGATACAAAAGCAACTCATGTTACTACTCAAGTGCGTGGTACCATGGGTCATATTGCCCCTGAATATTTGTCCACTGGAAAATCTTCAGAAAAGACTGATGTTTTTGGATATGGTATCACACTTCTCGAACTTGTCACTGGTCAGCGTGCCATAGATTTTGCTCGGCTTGAAGAAGAGGAGGATGTTCTTCTGCTTGACCATATCAAGAAGCTGCTGAGAGAAAATAGACTTGATGACATTGTGGATGGAAATATGAAGACCTATGATGAAAAAGAAGTTGGTACTGTCATTCAGGTTGCATTGCTCTGCACCCAGAGCTCACCTGAGGACCGTCCAACAATGGCTGAAGTGGTTAGATTGCTGCAGGGAGTGGATCTAGCAGAGAGATGGGCTGAGTGGGAGCAACTTGAAGATGTAAGGAGTCGTGAATTCTCACTCTTGTCGCACCAGTTTGCATGGGCTGAGGAGACTACACATGATCAAGTAGCTATACAGTTGTCCAAGGCAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

625

Amino Acids

69.62

Weight (kDa)

5.73

Isoelectric Point (pI)

29.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	42 - 79	5.3e-08	Leucine rich repeat N-terminal domain
LRR_14	PF23598	96 - 190	5e-07	Leucine-rich repeat region
LRR_8	PF13855	151 - 190	5.1e-06	Leucine rich repeat
Pkinase	PF00069	303 - 572	1.2e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	306 - 574	3.8e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63710 AT5G63710 AT5G63710 AT5G63710
fragaria_vesca	FvH4_1g25740 FvH4_1g25740 FvH4_1g25740 FvH4_1g25740 FvH4_1g25740 FvH4_1g25740 FvH4_1g25740 FvH4_1g25740 FvH4_1g25740 FvH4_1g25740 FvH4_1g25740 FvH4_1g25740
malus_domestica	MD15G1139900.v1.1
prunus_persica	Prupe.4G284100_v2.0.a1 Prupe.4G284400_v2.0.a1 Prupe.4G284400_v2.0.a1 Prupe.4G285600_v2.0.a1 Prupe.4G285600_v2.0.a1 Prupe.4G285600_v2.0.a1
pyrus_communis	pycom15g12640
rosa_chinensis	RchiOBHm_Chr3g0484651
rosa_laevigata	RLG00000023209
rosa_multiflora	Rmu_sc0005467.1_g000001 Rmu_sc0006192.1_g000001
rosa_roxburghii	Rroxscaffold_6G00397540
rosa_rugosa	Rorug03G0212900 Rorug03G0213000 Rorug03G0213100 Rorug03G0213200
rosa_samantha	Rh3AG263000 Rh3AG263200 Rh3BG298600 Rh3CG295700 Rh3CG295900 Rh3DG291900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	716
Acc65I	GGTACC	1 cut(s)	1400
AccB1I	GGYRCC	1 cut(s)	1400
AccB7I	CCANNNNNTGG	2 cut(s)	447, 1240
AccIII	TCCGGA	1 cut(s)	511
AciI	CCGC	1 cut(s)	824
AclWI	GGATC	2 cut(s)	558, 1743
AcoI	YGGCCR	1 cut(s)	172
AcsI	RAATTY	5 cut(s)	317, 579, 843, 882, 1790
AcuI	CTGAAG	5 cut(s)	22, 409, 818, 1431, 1728
AfaI	GTAC	4 cut(s)	1044, 1099, 1402, 1645
AfiI	CCNNNNNNNGG	5 cut(s)	103, 265, 447, 1240, 1334
AflII	CTTAAG	1 cut(s)	149
AgsI	TTSAA	8 cut(s)	125, 613, 652, 687, 887, 1291, 1532, 1772
AjnI	CCWGG	5 cut(s)	264, 330, 339, 1012, 1177
AjuI	GAANNNNNNNTTGG	6 cut(s)	169, 201, 856, 888, 1623, 1655
AloI	GAACNNNNNNTCC	2 cut(s)	280, 312
AluBI	AGCT	7 cut(s)	42, 219, 648, 1357, 1573, 1680, 1850
AluI	AGCT	7 cut(s)	42, 219, 648, 1357, 1573, 1680, 1850
Alw21I	GWGCWC	1 cut(s)	1682
Alw26I	GTCTC	2 cut(s)	228, 1823
AlwI	GGATC	2 cut(s)	558, 1743
Aor13HI	TCCGGA	1 cut(s)	511
AoxI	GGCC	1 cut(s)	172
ApeKI	GCWGC	5 cut(s)	1025, 1229, 1295, 1573, 1723
ApoI	RAATTY	5 cut(s)	317, 579, 843, 882, 1790
AseI	ATTAAT	1 cut(s)	137
Asp700I	GAANNNNTTC	3 cut(s)	292, 868, 1444
Asp718I	GGTACC	1 cut(s)	1400
AspLEI	GCGC	1 cut(s)	1215
AspS9I	GGNCC	3 cut(s)	431, 1117, 1690
AsuHPI	GGTGA	4 cut(s)	140, 515, 839, 1674
AsuNHI	GCTAGC	1 cut(s)	1348
AvaII	GGWCC	3 cut(s)	431, 1117, 1690
AxyI	CCTNAGG	1 cut(s)	1686
BanI	GGYRCC	1 cut(s)	1400
BanII	GRGCYC	2 cut(s)	209, 1682
BbsI	GAAGAC	1 cut(s)	1625
Bbv12I	GWGCWC	1 cut(s)	1682
BbvCI	CCTCAGC	1 cut(s)	1824
BbvI	GCAGC	5 cut(s)	1012, 1241, 1282, 1560, 1710
BccI	CCATC	3 cut(s)	56, 1601, 1743
BciT130I	CCWGG	5 cut(s)	266, 332, 341, 1014, 1179
BclI	TGATCA	1 cut(s)	1840
BcoDI	GTCTC	2 cut(s)	228, 1823
BfaI	CTAG	5 cut(s)	108, 645, 1310, 1349, 1739
BfmI	CTRYAG	1 cut(s)	1724
BfrI	CTTAAG	1 cut(s)	149
BglII	AGATCT	1 cut(s)	1168
BisI	GCNGC	5 cut(s)	1026, 1230, 1296, 1574, 1724
BlsI	GCNGC	5 cut(s)	1027, 1231, 1297, 1575, 1725
Bme1390I	CCNGG	5 cut(s)	266, 332, 341, 1014, 1179
Bme18I	GGWCC	3 cut(s)	431, 1117, 1690
BmgT120I	GGNCC	3 cut(s)	431, 1117, 1690
BmiI	GGNNCC	3 cut(s)	603, 1118, 1402
BmrFI	CCNGG	5 cut(s)	266, 332, 341, 1014, 1179
BmsI	GCATC	2 cut(s)	121, 278
BmtI	GCTAGC	1 cut(s)	1352
BpiI	GAAGAC	1 cut(s)	1625
BplI	GAGNNNNNCTC	2 cut(s)	945, 977
BpmI	CTGGAG	1 cut(s)	380
Bpu10I	CCTNAGC	2 cut(s)	260, 1824
BpuEI	CTTGAG	2 cut(s)	1183, 1374
Bsa29I	ATCGAT	2 cut(s)	141, 775
BsaBI	GATNNNNATC	2 cut(s)	8, 195
BsaI	GGTCTC	1 cut(s)	228
BsaJI	CCNNGG	4 cut(s)	77, 1234, 1404, 1863
BsaWI	WCCGGW	1 cut(s)	511
BsaXI	ACNNNNNCTCC	2 cut(s)	280, 310
Bsc4I	CCNNNNNNNGG	5 cut(s)	103, 265, 447, 1240, 1334
Bse1I	ACTGG	4 cut(s)	1202, 1441, 1504, 1810
Bse21I	CCTNAGG	1 cut(s)	1686
Bse3DI	GCAATG	2 cut(s)	1215, 1661
Bse8I	GATNNNNATC	2 cut(s)	8, 195
BseAI	TCCGGA	1 cut(s)	511
BseBI	CCWGG	5 cut(s)	266, 332, 341, 1014, 1179
BseCI	ATCGAT	2 cut(s)	141, 775
BseDI	CCNNGG	4 cut(s)	77, 1234, 1404, 1863
BseGI	GGATG	3 cut(s)	73, 1549, 1612
BseJI	GATNNNNATC	2 cut(s)	8, 195
BseLI	CCNNNNNNNGG	5 cut(s)	103, 265, 447, 1240, 1334
BseMI	GCAATG	2 cut(s)	1215, 1661
BseMII	CTCAG	9 cut(s)	251, 978, 1025, 1101, 1136, 1568, 1677, 1746, 1815
BseNI	ACTGG	4 cut(s)	1202, 1441, 1504, 1810
BseRI	GAGGAG	2 cut(s)	1553, 1841
BseXI	GCAGC	5 cut(s)	1012, 1241, 1282, 1560, 1710
BsgI	GTGCAG	2 cut(s)	1248, 1654
BshFI	GGCC	1 cut(s)	174
BshNI	GGYRCC	1 cut(s)	1400
BshVI	ATCGAT	2 cut(s)	141, 775
BsiHKAI	GWGCWC	1 cut(s)	1682
BsiSI	CCGG	1 cut(s)	512
BslI	CCNNNNNNNGG	5 cut(s)	103, 265, 447, 1240, 1334
BsmAI	GTCTC	2 cut(s)	228, 1823
BsmI	GAATGC	1 cut(s)	1028
BsnI	GGCC	1 cut(s)	174
Bso31I	GGTCTC	1 cut(s)	228
Bsp1286I	GDGCHC	2 cut(s)	209, 1682
Bsp13I	TCCGGA	1 cut(s)	511
Bsp1407I	TGTACA	1 cut(s)	1097
Bsp143I	GATC	4 cut(s)	550, 1168, 1735, 1840
Bsp19I	CCATGG	3 cut(s)	77, 1234, 1404
BspACI	CCGC	1 cut(s)	824
BspANI	GGCC	1 cut(s)	174
BspCNI	CTCAG	9 cut(s)	252, 977, 1026, 1102, 1137, 1569, 1678, 1747, 1816
BspDI	ATCGAT	2 cut(s)	141, 775
BspEI	TCCGGA	1 cut(s)	511
BspHI	TCATGA	1 cut(s)	1134
BspLI	GGNNCC	3 cut(s)	603, 1118, 1402
BspMAI	CTGCAG	1 cut(s)	1728
BspOI	GCTAGC	1 cut(s)	1352
BspPI	GGATC	2 cut(s)	558, 1743
BspQI	GCTCTTC	1 cut(s)	679
BspT107I	GGYRCC	1 cut(s)	1400
BspTI	CTTAAG	1 cut(s)	149
BspTNI	GGTCTC	1 cut(s)	228
BsrDI	GCAATG	2 cut(s)	1215, 1661
BsrGI	TGTACA	1 cut(s)	1097
BsrI	ACTGG	4 cut(s)	1202, 1441, 1504, 1810
BssECI	CCNNGG	4 cut(s)	77, 1234, 1404, 1863
BssMI	GATC	4 cut(s)	550, 1168, 1735, 1840
BssT1I	CCWWGG	4 cut(s)	77, 1234, 1404, 1863
Bst2UI	CCWGG	5 cut(s)	266, 332, 341, 1014, 1179
Bst4CI	ACNGT	9 cut(s)	601, 794, 839, 1010, 1083, 1283, 1648, 1694, 1857
Bst6I	CTCTTC	6 cut(s)	34, 599, 679, 703, 937, 1530
BstAFI	CTTAAG	1 cut(s)	149
BstAUI	TGTACA	1 cut(s)	1097
BstC8I	GCNNGC	2 cut(s)	1350, 1509
BstDSI	CCRYGG	3 cut(s)	77, 1234, 1404
BstF5I	GGATG	3 cut(s)	73, 1549, 1612
BstHHI	GCGC	1 cut(s)	1215
BstKTI	GATC	4 cut(s)	553, 1171, 1738, 1843
BstMAI	GTCTC	2 cut(s)	228, 1823
BstMBI	GATC	4 cut(s)	550, 1168, 1735, 1840
BstMWI	GCNNNNNNNGC	3 cut(s)	1226, 1354, 1677
BstNI	CCWGG	5 cut(s)	266, 332, 341, 1014, 1179
BstNSI	RCATGY	1 cut(s)	790
BstSCI	CCNGG	5 cut(s)	264, 330, 339, 1012, 1177
BstSFI	CTRYAG	1 cut(s)	1724
BstV1I	GCAGC	5 cut(s)	1012, 1241, 1282, 1560, 1710
BstV2I	GAAGAC	1 cut(s)	1625
BstX2I	RGATCY	3 cut(s)	550, 1168, 1735
BstYI	RGATCY	3 cut(s)	550, 1168, 1735
Bsu15I	ATCGAT	2 cut(s)	141, 775
Bsu36I	CCTNAGG	1 cut(s)	1686
BsuRI	GGCC	1 cut(s)	174
BsuTUI	ATCGAT	2 cut(s)	141, 775
BtgI	CCRYGG	3 cut(s)	77, 1234, 1404
BtsCI	GGATG	3 cut(s)	73, 1549, 1612
BtsI	GCAGTG	1 cut(s)	990
BtsIMutI	CAGTG	4 cut(s)	155, 990, 1434, 1497
Cac8I	GCNNGC	2 cut(s)	1350, 1509
CciI	TCATGA	1 cut(s)	1134
CfoI	GCGC	1 cut(s)	1215
Cfr13I	GGNCC	3 cut(s)	431, 1117, 1690
ClaI	ATCGAT	2 cut(s)	141, 775
Csp6I	GTAC	4 cut(s)	1043, 1098, 1401, 1644
CviQI	GTAC	4 cut(s)	1043, 1098, 1401, 1644
DpnI	GATC	4 cut(s)	552, 1170, 1737, 1842
DpnII	GATC	4 cut(s)	550, 1168, 1735, 1840
EaeI	YGGCCR	1 cut(s)	172
Eam1104I	CTCTTC	6 cut(s)	34, 599, 679, 703, 937, 1530
EarI	CTCTTC	6 cut(s)	34, 599, 679, 703, 937, 1530
Ecl136II	GAGCTC	1 cut(s)	1680
Eco130I	CCWWGG	4 cut(s)	77, 1234, 1404, 1863
Eco24I	GRGCYC	2 cut(s)	209, 1682
Eco31I	GGTCTC	1 cut(s)	228
Eco32I	GATATC	1 cut(s)	773
Eco47I	GGWCC	3 cut(s)	431, 1117, 1690
Eco53kI	GAGCTC	1 cut(s)	1680
Eco57I	CTGAAG	5 cut(s)	22, 409, 818, 1431, 1728
Eco81I	CCTNAGG	1 cut(s)	1686
EcoICRI	GAGCTC	1 cut(s)	1680
EcoO109I	RGGNCCY	2 cut(s)	431, 1117
EcoRI	GAATTC	1 cut(s)	1790
EcoRII	CCWGG	5 cut(s)	264, 330, 339, 1012, 1177
EcoRV	GATATC	1 cut(s)	773
EcoT14I	CCWWGG	4 cut(s)	77, 1234, 1404, 1863
EcoT22I	ATGCAT	1 cut(s)	136
EcoT38I	GRGCYC	2 cut(s)	209, 1682
ErhI	CCWWGG	4 cut(s)	77, 1234, 1404, 1863
FalI	AAGNNNNNCTT	2 cut(s)	856, 888
FauI	CCCGC	1 cut(s)	817
FbaI	TGATCA	1 cut(s)	1840
Fnu4HI	GCNGC	5 cut(s)	1026, 1230, 1296, 1574, 1724
FokI	GGATG	3 cut(s)	60, 1556, 1619
FriOI	GRGCYC	2 cut(s)	209, 1682
Fsp4HI	GCNGC	5 cut(s)	1026, 1230, 1296, 1574, 1724
FspBI	CTAG	5 cut(s)	108, 645, 1310, 1349, 1739
GlaI	GCGC	1 cut(s)	1214
GluI	GCNGC	5 cut(s)	1026, 1230, 1296, 1574, 1724
GsuI	CTGGAG	1 cut(s)	380
HaeIII	GGCC	1 cut(s)	174
HapII	CCGG	1 cut(s)	512
HhaI	GCGC	1 cut(s)	1215
Hin6I	GCGC	1 cut(s)	1213
HinP1I	GCGC	1 cut(s)	1213
HincII	GTYRAC	2 cut(s)	455, 1203
HindII	GTYRAC	2 cut(s)	455, 1203
HinfI	GANTC	5 cut(s)	164, 400, 515, 1072, 1783
HpaII	CCGG	1 cut(s)	512
HphI	GGTGA	4 cut(s)	140, 515, 839, 1674
Hpy166II	GTNNAC	4 cut(s)	455, 949, 1203, 1434
Hpy188I	TCNGA	7 cut(s)	428, 606, 798, 967, 1111, 1173, 1450
Hpy8I	GTNNAC	4 cut(s)	455, 949, 1203, 1434
HpyAV	CCTTC	4 cut(s)	43, 119, 309, 1285
HpyCH4III	ACNGT	9 cut(s)	601, 794, 839, 1010, 1083, 1283, 1648, 1694, 1857
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1226, 1354, 1677
HspAI	GCGC	1 cut(s)	1213
Kpn2I	TCCGGA	1 cut(s)	511
KpnI	GGTACC	1 cut(s)	1404
Ksp22I	TGATCA	1 cut(s)	1840
Kzo9I	GATC	4 cut(s)	550, 1168, 1735, 1840
LguI	GCTCTTC	1 cut(s)	679
LmnI	GCTCC	1 cut(s)	1762
Lsp1109I	GCAGC	5 cut(s)	1012, 1241, 1282, 1560, 1710
LweI	GCATC	2 cut(s)	121, 278
MaeI	CTAG	5 cut(s)	108, 645, 1310, 1349, 1739
MaeIII	GTNAC	7 cut(s)	229, 363, 736, 914, 1283, 1381, 1495
MalI	GATC	4 cut(s)	552, 1170, 1737, 1842
MboI	GATC	4 cut(s)	550, 1168, 1735, 1840
MfeI	CAATTG	1 cut(s)	857
MflI	RGATCY	3 cut(s)	550, 1168, 1735
MhlI	GDGCHC	2 cut(s)	209, 1682
MlyI	GAGTC	1 cut(s)	1792
MmeI	TCCRAC	2 cut(s)	629, 1721
Mph1103I	ATGCAT	1 cut(s)	136
MroI	TCCGGA	1 cut(s)	511
MroXI	GAANNNNTTC	3 cut(s)	292, 868, 1444
MseI	TTAA	3 cut(s)	21, 137, 150
MspA1I	CMGCKG	2 cut(s)	42, 1357
MspCI	CTTAAG	1 cut(s)	149
MspI	CCGG	1 cut(s)	512
MspR9I	CCNGG	5 cut(s)	266, 332, 341, 1014, 1179
MunI	CAATTG	1 cut(s)	857
Mva1269I	GAATGC	1 cut(s)	1028
MvaI	CCWGG	5 cut(s)	266, 332, 341, 1014, 1179
MwoI	GCNNNNNNNGC	3 cut(s)	1226, 1354, 1677
NcoI	CCATGG	3 cut(s)	77, 1234, 1404
NdeII	GATC	4 cut(s)	550, 1168, 1735, 1840
NheI	GCTAGC	1 cut(s)	1348
NlaIV	GGNNCC	3 cut(s)	603, 1118, 1402
NmeAIII	GCCGAG	1 cut(s)	1504
NmuCI	GTSAC	4 cut(s)	229, 736, 1283, 1495
NsiI	ATGCAT	1 cut(s)	136
NspI	RCATGY	1 cut(s)	790
PagI	TCATGA	1 cut(s)	1134
PciSI	GCTCTTC	1 cut(s)	679
PctI	GAATGC	1 cut(s)	1028
PdmI	GAANNNNTTC	3 cut(s)	292, 868, 1444
PfeI	GAWTC	4 cut(s)	164, 400, 515, 1072
PflMI	CCANNNNNTGG	2 cut(s)	447, 1240
PfoI	TCCNGGA	1 cut(s)	339
PkrI	GCNGC	5 cut(s)	1027, 1231, 1297, 1575, 1725
PleI	GAGTC	1 cut(s)	1791
PpsI	GAGTC	1 cut(s)	1791
PpuMI	RGGWCCY	2 cut(s)	431, 1117
PshBI	ATTAAT	1 cut(s)	137
PsiI	TTATAA	1 cut(s)	716
Psp124BI	GAGCTC	1 cut(s)	1682
Psp5II	RGGWCCY	2 cut(s)	431, 1117
Psp6I	CCWGG	5 cut(s)	264, 330, 339, 1012, 1177
PspGI	CCWGG	5 cut(s)	264, 330, 339, 1012, 1177
PspN4I	GGNNCC	3 cut(s)	603, 1118, 1402
PspPI	GGNCC	3 cut(s)	431, 1117, 1690
PspPPI	RGGWCCY	2 cut(s)	431, 1117
PsrI	GAACNNNNNNTAC	2 cut(s)	616, 648
PstI	CTGCAG	1 cut(s)	1728
PsuI	RGATCY	3 cut(s)	550, 1168, 1735
PvuII	CAGCTG	2 cut(s)	42, 1357
RsaI	GTAC	4 cut(s)	1044, 1099, 1402, 1645
RsaNI	GTAC	4 cut(s)	1043, 1098, 1401, 1644
SacI	GAGCTC	1 cut(s)	1682
SapI	GCTCTTC	1 cut(s)	679
SaqAI	TTAA	3 cut(s)	21, 137, 150
SatI	GCNGC	5 cut(s)	1026, 1230, 1296, 1574, 1724
Sau3AI	GATC	4 cut(s)	550, 1168, 1735, 1840
Sau96I	GGNCC	3 cut(s)	431, 1117, 1690
SchI	GAGTC	1 cut(s)	1792
ScrFI	CCNGG	5 cut(s)	266, 332, 341, 1014, 1179
SduI	GDGCHC	2 cut(s)	209, 1682
SfaNI	GCATC	2 cut(s)	121, 278
SfcI	CTRYAG	1 cut(s)	1724
SinI	GGWCC	3 cut(s)	431, 1117, 1690
SmlI	CTYRAG	3 cut(s)	149, 1162, 1389
SmoI	CTYRAG	3 cut(s)	149, 1162, 1389
SsiI	CCGC	1 cut(s)	824
SspI	AATATT	1 cut(s)	1427
SspMI	CTAG	5 cut(s)	108, 645, 1310, 1349, 1739
SstI	GAGCTC	1 cut(s)	1682
StyD4I	CCNGG	5 cut(s)	264, 330, 339, 1012, 1177
StyI	CCWWGG	4 cut(s)	77, 1234, 1404, 1863
TaaI	ACNGT	9 cut(s)	601, 794, 839, 1010, 1083, 1283, 1648, 1694, 1857
TaqI	TCGA	5 cut(s)	141, 775, 906, 1362, 1488
TatI	WGTACW	2 cut(s)	1042, 1097
TfiI	GAWTC	4 cut(s)	164, 400, 515, 1072
Tru1I	TTAA	3 cut(s)	21, 137, 150
Tru9I	TTAA	3 cut(s)	21, 137, 150
TscAI	CASTG	4 cut(s)	162, 990, 1441, 1504
TseFI	GTSAC	4 cut(s)	229, 736, 1283, 1495
TseI	GCWGC	5 cut(s)	1025, 1229, 1295, 1573, 1723
Tsp45I	GTSAC	4 cut(s)	229, 736, 1283, 1495
TspDTI	ATGAA	8 cut(s)	185, 546, 1055, 1095, 1123, 1151, 1631, 1644
TspGWI	ACGGA	1 cut(s)	1119
TspRI	CASTG	4 cut(s)	162, 990, 1441, 1504
Van91I	CCANNNNNTGG	2 cut(s)	447, 1240
Vha464I	CTTAAG	1 cut(s)	149
VpaK11BI	GGWCC	3 cut(s)	431, 1117, 1690
VspI	ATTAAT	1 cut(s)	137
XapI	RAATTY	5 cut(s)	317, 579, 843, 882, 1790
XbaI	TCTAGA	1 cut(s)	1309
XceI	RCATGY	1 cut(s)	790
XcmI	CCANNNNNNNNNTGG	1 cut(s)	216
XmnI	GAANNNNTTC	3 cut(s)	292, 868, 1444
XspI	CTAG	5 cut(s)	108, 645, 1310, 1349, 1739
Zsp2I	ATGCAT	1 cut(s)	136

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.