Rorug03G0213000
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002712
Physical Location & Seq
Reverse (-)
19209238 .. 19211134
1897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0213000.1

Sequence Viewer

Length: 633 bp
ATGTCTTCAAAAGACTGCGGCAACCACGGCCACAAGATGAGGAGAAAGAGAATCCGCCGCTGCTGCGCTGGGCTCCTCATCTTCAACTTCATCCTCCTCGTCACCATCCTCATCATCTGGGCCATCCTCCAGCCGTCCAAGCCCCGCTTCATCCTCCAGGACGTCACCGTCTACAACTTCAACAACACCGTCCCCAACGTCTTCTCCTCCAGCTTCCAGGTCACCATCTCCTCTCGCAACCCCAACGACAAGCTCGGAATCTACTACGACAAGCTCGACGTCTACGCCACCTACCGCGCCCAGCAGATCACCTTCTCAACCCTAATCCCGCCCACTTACCAAGGCCACAAGGACGTCAACATCTGGTCGCCCTTCATCTACGGCACCGACGTCCCCATCGCTCCCTACAACTCCATTGCTCTCGGACAGGACCAGGCCGCCGGCACCGTACTTTTGTTGATCAAGATTGACGGCCGGGTGAGATGGAAGATTGGAACATTCATCACCGGGAAGTACCATCTCTATGTGAGATGTCCGGCTTTCATAACTTTGGGAGCCAAGACTACCGGAATTGTGGTCGGAGAAAACGCTGTCAAGTACCAGCTGGTCCAGCGATGCAAGGTTAGCGTATGA

Protein Analysis

210

Amino Acids

23.72

Weight (kDa)

9.82

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 75 - 177 8.2e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AatII GACGTC 4 cut(s) 165, 282, 357, 393
AccB1I GGYRCC 2 cut(s) 383, 443
AccI GTMKAC 2 cut(s) 171, 282
AccII CGCG 1 cut(s) 297
AciI CCGC 7 cut(s) 18, 55, 58, 145, 295, 329, 438
AcoI YGGCCR 2 cut(s) 28, 472
AcyI GRCGYC 4 cut(s) 162, 279, 354, 390
AfaI GTAC 3 cut(s) 450, 515, 599
AgsI TTSAA 3 cut(s) 9, 85, 181
AjnI CCWGG 3 cut(s) 156, 216, 432
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AluBI AGCT 4 cut(s) 213, 253, 274, 604
AluI AGCT 4 cut(s) 213, 253, 274, 604
AoxI GGCC 5 cut(s) 28, 120, 343, 435, 472
ApeKI GCWGC 2 cut(s) 60, 63
AspLEI GCGC 2 cut(s) 68, 299
AspS9I GGNCC 3 cut(s) 120, 430, 607
AsuC2I CCSGG 2 cut(s) 476, 508
AsuHPI GGTGA 6 cut(s) 94, 157, 214, 301, 490, 496
AvaII GGWCC 2 cut(s) 430, 607
BanI GGYRCC 2 cut(s) 383, 443
BanII GRGCYC 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 193
BbvI GCAGC 2 cut(s) 47, 50
BccI CCATC 6 cut(s) 113, 131, 233, 404, 477, 525
BceAI ACGGC 4 cut(s) 43, 118, 397, 487
BciT130I CCWGG 3 cut(s) 158, 218, 434
BclI TGATCA 1 cut(s) 459
BcnI CCSGG 2 cut(s) 476, 508
BisI GCNGC 5 cut(s) 19, 58, 61, 64, 438
BlsI GCNGC 5 cut(s) 20, 59, 62, 65, 439
Bme1390I CCNGG 5 cut(s) 158, 218, 434, 476, 508
Bme18I GGWCC 2 cut(s) 430, 607
BmgT120I GGNCC 3 cut(s) 120, 430, 607
BmiI GGNNCC 4 cut(s) 74, 385, 445, 556
BmrFI CCNGG 5 cut(s) 158, 218, 434, 476, 508
BmsI GCATC 1 cut(s) 605
BpiI GAAGAC 1 cut(s) 193
BpmI CTGGAG 3 cut(s) 113, 140, 193
BpuMI CCSGG 2 cut(s) 476, 508
BsaHI GRCGYC 4 cut(s) 162, 279, 354, 390
BsaJI CCNNGG 2 cut(s) 25, 340
BsaWI WCCGGW 1 cut(s) 566
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bse118I RCCGGY 1 cut(s) 440
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 3 cut(s) 158, 218, 434
BseDI CCNNGG 2 cut(s) 25, 340
BseGI GGATG 4 cut(s) 90, 105, 123, 150
BseMI GCAATG 1 cut(s) 414
BseRI GAGGAG 5 cut(s) 55, 65, 86, 196, 220
BseX3I CGGCCG 1 cut(s) 472
BseXI GCAGC 2 cut(s) 47, 50
BseYI CCCAGC 2 cut(s) 68, 300
Bsh1236I CGCG 1 cut(s) 297
Bsh1285I CGRYCG 1 cut(s) 475
BshFI GGCC 5 cut(s) 30, 122, 345, 437, 474
BshNI GGYRCC 2 cut(s) 383, 443
BsiEI CGRYCG 1 cut(s) 475
BsiSI CCGG 5 cut(s) 441, 475, 507, 536, 567
BslFI GGGAC 2 cut(s) 176, 377
BsmFI GGGAC 2 cut(s) 176, 377
BsnI GGCC 5 cut(s) 30, 122, 345, 437, 474
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 2 cut(s) 306, 459
BspACI CCGC 7 cut(s) 18, 55, 58, 145, 295, 329, 438
BspANI GGCC 5 cut(s) 30, 122, 345, 437, 474
BspFNI CGCG 1 cut(s) 297
BspLI GGNNCC 4 cut(s) 74, 385, 445, 556
BspT107I GGYRCC 2 cut(s) 383, 443
BsrDI GCAATG 1 cut(s) 414
BsrFI RCCGGY 1 cut(s) 440
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 2 cut(s) 25, 340
BssMI GATC 2 cut(s) 306, 459
BssNI GRCGYC 4 cut(s) 162, 279, 354, 390
BssT1I CCWWGG 1 cut(s) 340
Bst2UI CCWGG 3 cut(s) 158, 218, 434
Bst4CI ACNGT 3 cut(s) 169, 190, 448
BstACI GRCGYC 4 cut(s) 162, 279, 354, 390
BstC8I GCNNGC 1 cut(s) 442
BstDSI CCRYGG 1 cut(s) 25
BstEII GGTNACC 1 cut(s) 220
BstF5I GGATG 4 cut(s) 90, 105, 123, 150
BstFNI CGCG 1 cut(s) 297
BstHHI GCGC 2 cut(s) 68, 299
BstKTI GATC 2 cut(s) 309, 462
BstMBI GATC 2 cut(s) 306, 459
BstMCI CGRYCG 1 cut(s) 475
BstMWI GCNNNNNNNGC 5 cut(s) 27, 63, 139, 610, 624
BstNI CCWGG 3 cut(s) 158, 218, 434
BstPI GGTNACC 1 cut(s) 220
BstSCI CCNGG 5 cut(s) 156, 216, 432, 474, 506
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 2 cut(s) 47, 50
BstV2I GAAGAC 1 cut(s) 193
BstZI CGGCCG 1 cut(s) 472
BsuRI GGCC 5 cut(s) 30, 122, 345, 437, 474
BtgI CCRYGG 1 cut(s) 25
BtgZI GCGATG 2 cut(s) 382, 628
BtsCI GGATG 4 cut(s) 90, 105, 123, 150
Cac8I GCNNGC 1 cut(s) 442
CfoI GCGC 2 cut(s) 68, 299
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 3 cut(s) 120, 430, 607
Csp6I GTAC 3 cut(s) 449, 514, 598
CviQI GTAC 3 cut(s) 449, 514, 598
DpnI GATC 2 cut(s) 308, 461
DpnII GATC 2 cut(s) 306, 459
DrdI GACNNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 2 cut(s) 28, 472
EagI CGGCCG 1 cut(s) 472
EciI GGCGGA 1 cut(s) 44
EclXI CGGCCG 1 cut(s) 472
Eco130I CCWWGG 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 430, 607
Eco52I CGGCCG 1 cut(s) 472
Eco91I GGTNACC 1 cut(s) 220
EcoO65I GGTNACC 1 cut(s) 220
EcoRII CCWGG 3 cut(s) 156, 216, 432
EcoT14I CCWWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 340
FaiI YATR 3 cut(s) 525, 545, 631
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FaqI GGGAC 2 cut(s) 176, 377
FauI CCCGC 2 cut(s) 152, 336
FbaI TGATCA 1 cut(s) 459
FblI GTMKAC 2 cut(s) 171, 282
Fnu4HI GCNGC 5 cut(s) 19, 58, 61, 64, 438
FokI GGATG 4 cut(s) 77, 92, 110, 137
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 5 cut(s) 19, 58, 61, 64, 438
GlaI GCGC 2 cut(s) 67, 298
GluI GCNGC 5 cut(s) 19, 58, 61, 64, 438
GsaI CCCAGC 2 cut(s) 72, 304
GsuI CTGGAG 3 cut(s) 113, 140, 193
HaeIII GGCC 5 cut(s) 30, 122, 345, 437, 474
HapII CCGG 5 cut(s) 441, 475, 507, 536, 567
HhaI GCGC 2 cut(s) 68, 299
Hin1I GRCGYC 4 cut(s) 162, 279, 354, 390
Hin6I GCGC 2 cut(s) 66, 297
HinP1I GCGC 2 cut(s) 66, 297
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 2 cut(s) 51, 258
HpaII CCGG 5 cut(s) 441, 475, 507, 536, 567
HphI GGTGA 6 cut(s) 94, 157, 214, 301, 490, 496
Hpy166II GTNNAC 3 cut(s) 172, 283, 358
Hpy188I TCNGA 3 cut(s) 257, 425, 581
Hpy188III TCNNGA 1 cut(s) 463
Hpy8I GTNNAC 3 cut(s) 172, 283, 358
Hpy99I CGWCG 2 cut(s) 281, 392
HpyAV CCTTC 2 cut(s) 322, 382
HpyCH4III ACNGT 3 cut(s) 169, 190, 448
HpyCH4IV ACGT 5 cut(s) 162, 198, 279, 354, 390
HpyCH4V TGCA 1 cut(s) 618
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 63, 139, 610, 624
HpySE526I ACGT 5 cut(s) 162, 198, 279, 354, 390
Hsp92I GRCGYC 4 cut(s) 162, 279, 354, 390
HspAI GCGC 2 cut(s) 66, 297
KroI GCCGGC 1 cut(s) 440
KroNI GCCGGC 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 2 cut(s) 306, 459
LmnI GCTCC 3 cut(s) 78, 406, 554
Lsp1109I GCAGC 2 cut(s) 47, 50
LweI GCATC 1 cut(s) 605
MaeII ACGT 5 cut(s) 162, 198, 279, 354, 390
MaeIII GTNAC 3 cut(s) 100, 163, 220
MalI GATC 2 cut(s) 308, 461
MboI GATC 2 cut(s) 306, 459
MboII GAAGA 3 cut(s) 73, 193, 499
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 1 cut(s) 570
MmeI TCCRAC 1 cut(s) 559
MnlI CCTC 9 cut(s) 33, 86, 104, 107, 119, 137, 164, 217, 241
MroNI GCCGGC 1 cut(s) 440
MslI CAYNNNNRTG 1 cut(s) 522
MspA1I CMGCKG 2 cut(s) 60, 604
MspI CCGG 5 cut(s) 441, 475, 507, 536, 567
MspR9I CCNGG 5 cut(s) 158, 218, 434, 476, 508
MvaI CCWGG 3 cut(s) 158, 218, 434
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 5 cut(s) 27, 63, 139, 610, 624
NaeI GCCGGC 1 cut(s) 442
NciI CCSGG 2 cut(s) 476, 508
NdeII GATC 2 cut(s) 306, 459
NgoMIV GCCGGC 1 cut(s) 440
NlaIV GGNNCC 4 cut(s) 74, 385, 445, 556
NmuCI GTSAC 3 cut(s) 100, 163, 220
PcsI WCGNNNNNNNCGW 2 cut(s) 273, 387
PdiI GCCGGC 1 cut(s) 442
PfeI GAWTC 2 cut(s) 51, 258
PfoI TCCNGGA 1 cut(s) 156
PkrI GCNGC 5 cut(s) 20, 59, 62, 65, 439
Psp6I CCWGG 3 cut(s) 156, 216, 432
PspEI GGTNACC 1 cut(s) 220
PspFI CCCAGC 2 cut(s) 68, 300
PspGI CCWGG 3 cut(s) 156, 216, 432
PspN4I GGNNCC 4 cut(s) 74, 385, 445, 556
PspPI GGNCC 3 cut(s) 120, 430, 607
PvuII CAGCTG 1 cut(s) 604
RsaI GTAC 3 cut(s) 450, 515, 599
RsaNI GTAC 3 cut(s) 449, 514, 598
RseI CAYNNNNRTG 1 cut(s) 522
SatI GCNGC 5 cut(s) 19, 58, 61, 64, 438
Sau3AI GATC 2 cut(s) 306, 459
Sau96I GGNCC 3 cut(s) 120, 430, 607
ScrFI CCNGG 5 cut(s) 158, 218, 434, 476, 508
SduI GDGCHC 1 cut(s) 75
SfaNI GCATC 1 cut(s) 605
SinI GGWCC 2 cut(s) 430, 607
SmiMI CAYNNNNRTG 1 cut(s) 522
Sse9I AATT 1 cut(s) 570
SsiI CCGC 7 cut(s) 18, 55, 58, 145, 295, 329, 438
StyD4I CCNGG 5 cut(s) 156, 216, 432, 474, 506
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 3 cut(s) 169, 190, 448
TaiI ACGT 5 cut(s) 165, 201, 282, 357, 393
TaqI TCGA 1 cut(s) 276
TasI AATT 1 cut(s) 570
TauI GCSGC 3 cut(s) 21, 60, 440
TfiI GAWTC 2 cut(s) 51, 258
TseFI GTSAC 3 cut(s) 100, 163, 220
TseI GCWGC 2 cut(s) 60, 63
Tsp45I GTSAC 3 cut(s) 100, 163, 220
TspDTI ATGAA 5 cut(s) 79, 139, 364, 490, 532
VpaK11BI GGWCC 2 cut(s) 430, 607
XmiI GTMKAC 2 cut(s) 171, 282
ZraI GACGTC 4 cut(s) 163, 280, 355, 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.